Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_I12
(900 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204; Euka... 131 2e-29
UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4; Bilateria|... 119 1e-25
UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3; Eukary... 114 3e-24
UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56; Euk... 114 3e-24
UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2; Ost... 107 4e-22
UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3; Eukary... 71 3e-11
UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1; Enceph... 66 2e-09
UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1; ... 62 1e-08
UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole ge... 57 5e-07
UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiel... 51 5e-05
UniRef50_Q9LPV3 Cluster: Putative 60S ribosomal protein L27a-1; ... 46 0.001
UniRef50_P53143 Cluster: Putative uncharacterized protein YGL102... 44 0.004
UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S riboso... 39 0.20
UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1; Flavobacteri... 34 5.7
UniRef50_Q6LXD1 Cluster: 50S ribosomal protein L15P; n=9; Euryar... 34 5.7
UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse t... 33 9.9
UniRef50_Q2H6Q7 Cluster: Putative uncharacterized protein; n=1; ... 33 9.9
>UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204;
Eukaryota|Rep: 60S ribosomal protein L27a - Homo sapiens
(Human)
Length = 148
Score = 131 bits (317), Expect = 2e-29
Identities = 62/141 (43%), Positives = 84/141 (59%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 259
M + +KTRKLRGHVS NAGG HHHRIN DKYHPGYFGK+GM++
Sbjct: 1 MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKH 60
Query: 260 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPAHQYCQSWILQVARQRQTPQT 439
+H ++N++FCP +NLDKLWTLVSEQTR+ A G P +S +V + + P+
Sbjct: 61 YHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQ 120
Query: 440 TCHSKSKVLLKISREENQGCG 502
K+K + + E+ + G
Sbjct: 121 PVIVKAKFFSRRAEEKIKSVG 141
Score = 34.3 bits (75), Expect = 4.3
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +3
Query: 372 SPLINIVKAXXXXXXXXXXXPKQPVIVXXXXXXXXXXXXXXDVGGACVLSA 524
+P+I++V++ PKQPVIV VGGACVL A
Sbjct: 98 APIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA 148
>UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4;
Bilateria|Rep: Ribosomal protein L27a - Ascaris suum
(Pig roundworm) (Ascaris lumbricoides)
Length = 133
Score = 119 bits (286), Expect = 1e-25
Identities = 54/108 (50%), Positives = 71/108 (65%)
Frame = +2
Query: 179 NAGGEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASA 358
NAGG+HHHRIN DKYHPGYFGK+GMRNFH RKN +CP +NLDKLW+LVSE+TR YA
Sbjct: 20 NAGGQHHHRINFDKYHPGYFGKVGMRNFHVRKNGLYCPTVNLDKLWSLVSEKTRTTYAEK 79
Query: 359 PDGKVPAHQYCQSWILQVARQRQTPQTTCHSKSKVLLKISREENQGCG 502
D K P ++ +V + P+ K+K + + ++ +G G
Sbjct: 80 KD-KAPVIDVVKAGYFKVLGKGLLPKQPVIVKAKFFSRTAEQKIKGVG 126
Score = 36.3 bits (80), Expect = 1.1
Identities = 19/52 (36%), Positives = 25/52 (48%)
Frame = +3
Query: 369 RSPLINIVKAXXXXXXXXXXXPKQPVIVXXXXXXXXXXXXXXDVGGACVLSA 524
++P+I++VKA PKQPVIV VGGAC+L A
Sbjct: 82 KAPVIDVVKAGYFKVLGKGLLPKQPVIVKAKFFSRTAEQKIKGVGGACILVA 133
>UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3;
Eukaryota|Rep: 60S ribosomal protein L27a - Tetrahymena
thermophila
Length = 149
Score = 114 bits (274), Expect = 3e-24
Identities = 63/149 (42%), Positives = 80/149 (53%), Gaps = 3/149 (2%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXN--AGGEHHHRINMDKYHPGYFGKLGM 253
M + KKTRKLRGHVS AGG HHHRI M+K+HPGY+GKLGM
Sbjct: 1 MVSHLKKTRKLRGHVSHGHGRVGKHRKGGCRGGRGKAGGMHHHRILMEKWHPGYYGKLGM 60
Query: 254 RNFHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPAHQYCQSWILQVARQRQTP 433
R FH +KN CPV+N+DKLW+LVS+ TR KYA KVP ++ +V + + P
Sbjct: 61 RTFHLKKNPLHCPVVNIDKLWSLVSDATRQKYAE-DKKKVPVIDVTKAGFFKVLGKGRLP 119
Query: 434 QTTCHSKSKVLLKISREENQGCGW-CLCT 517
K+K K + G C+ T
Sbjct: 120 NQPVVVKAKYFSKTAERRIVAVGGACVLT 148
>UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56;
Eukaryota|Rep: 60S ribosomal protein L27a-2 -
Arabidopsis thaliana (Mouse-ear cress)
Length = 146
Score = 114 bits (274), Expect = 3e-24
Identities = 62/142 (43%), Positives = 80/142 (56%), Gaps = 1/142 (0%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 259
MAT+ KK RK RGHVS NAGG HHHRI DKYHPGYFGK+GMR
Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
Query: 260 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPAHQYCQSWILQVARQRQTPQT 439
FH +NK FCP++NLDKLW+LV E + K S+ D VP Q +V + P+
Sbjct: 61 FHKLRNKFFCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPEN 117
Query: 440 TCH-SKSKVLLKISREENQGCG 502
K+K++ K + ++ + G
Sbjct: 118 KPFVVKAKLISKTAEKKIKEAG 139
>UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2;
Ostreococcus|Rep: 60s ribosomal protein L15/L27 -
Ostreococcus tauri
Length = 198
Score = 107 bits (257), Expect = 4e-22
Identities = 56/145 (38%), Positives = 76/145 (52%), Gaps = 1/145 (0%)
Frame = +2
Query: 86 TSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRNFH 265
T K RK RGHVS AGG+HHHRI MDKYHPGYFGK+GMR+FH
Sbjct: 54 TQLHKNRKKRGHVSAGHGRIGKHRKHPAGRGMAGGQHHHRIMMDKYHPGYFGKVGMRHFH 113
Query: 266 FRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPAHQYCQSWILQVARQRQTPQTTC 445
KN+ CP +N+DKLWTLV + R + A + P I ++ + Q P
Sbjct: 114 KLKNQTVCPTINIDKLWTLVGTEKREECAK-NTSQAPVIDLTDHGIFKLLGKGQLPNQPV 172
Query: 446 HSKSKVLLKISREE-NQGCGWCLCT 517
K + + K++ ++ + G C+ T
Sbjct: 173 VVKCRYISKLAEKKIKEVGGACILT 197
>UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3;
Eukaryota|Rep: 60S ribosomal protein L27A - Guillardia
theta (Cryptomonas phi)
Length = 144
Score = 71.3 bits (167), Expect = 3e-11
Identities = 47/147 (31%), Positives = 69/147 (46%), Gaps = 1/147 (0%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 259
M T +++RK RGHVS N+GG+HHH+I +KYH +FGK GMR
Sbjct: 1 MPTDIRRSRKKRGHVSSGHGRVGKHRKHPGGRGNSGGQHHHKIFFEKYHKKHFGKTGMRY 60
Query: 260 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPAHQYCQSWILQVARQRQTP-Q 436
+ K NF +NLDK+ + + L ++ D S +L R Q P
Sbjct: 61 YRKLKQNNFNKSINLDKISDKLVKNMHLHTKNSSD-SFDLSLIGMSKVLGRGRMVQKPFV 119
Query: 437 TTCHSKSKVLLKISREENQGCGWCLCT 517
+S SKV +K ++ G+C+ T
Sbjct: 120 IKANSFSKVAVKKIKKAG---GYCILT 143
>UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1;
Encephalitozoon cuniculi|Rep: 60S ribosomal protein L27a
- Encephalitozoon cuniculi
Length = 147
Score = 65.7 bits (153), Expect = 2e-09
Identities = 33/99 (33%), Positives = 50/99 (50%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 259
M KKTRKLRGHVS AGG H + ++HP Y GK GMR
Sbjct: 1 MTDRVKKTRKLRGHVSHGYGRVGKHRKHSGGRGLAGGFSHMKTFFTRFHPDYHGKRGMRV 60
Query: 260 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVP 376
+H ++N ++ ++ +LW ++ ++ R + P+ KVP
Sbjct: 61 YHRKENSDYARPISSARLWGMIPKEQRYDFLDNPE-KVP 98
>UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 187
Score = 62.5 bits (145), Expect = 1e-08
Identities = 33/105 (31%), Positives = 56/105 (53%)
Frame = +2
Query: 188 GEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDG 367
G ++ + YFGK+GMR+FH +N +F PV+NLDKLWTLV + R K +A
Sbjct: 77 GSQEMEVDREIGRDSYFGKVGMRHFHLTRNSSFRPVVNLDKLWTLVPAEQR-KGLTADST 135
Query: 368 KVPAHQYCQSWILQVARQRQTPQTTCHSKSKVLLKISREENQGCG 502
+VP + +V + + P C K++ + ++ ++ + G
Sbjct: 136 EVPVIDTLAAGYGKVLGKGRLPALPCIVKARWVSSLAEKKIKEAG 180
Score = 52.4 bits (120), Expect = 2e-05
Identities = 25/50 (50%), Positives = 26/50 (52%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHP 229
M T KTRK RGHVS AGG+HHHR NMDKYHP
Sbjct: 1 MPTHLSKTRKHRGHVSAGNGRVGKHRKHPGGRGLAGGQHHHRTNMDKYHP 50
>UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole
genome shotgun sequence; n=3; Vitis vinifera|Rep:
Chromosome chr12 scaffold_159, whole genome shotgun
sequence - Vitis vinifera (Grape)
Length = 151
Score = 57.2 bits (132), Expect = 5e-07
Identities = 21/37 (56%), Positives = 31/37 (83%)
Frame = +2
Query: 215 DKYHPGYFGKLGMRNFHFRKNKNFCPVLNLDKLWTLV 325
DK+HPG FGK+GMR+FH + K CP++++DKLW++V
Sbjct: 28 DKHHPGNFGKVGMRHFHKLRIKLHCPIVHIDKLWSMV 64
>UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiella
natans|Rep: Ribosomal protein L27a - Bigelowiella natans
(Pedinomonas minutissima) (Chlorarachnion sp.(strain
CCMP 621))
Length = 151
Score = 50.8 bits (116), Expect = 5e-05
Identities = 24/68 (35%), Positives = 33/68 (48%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 259
M T KK+RK+RG S +GG+HHH+I ++Y YF K GMR
Sbjct: 1 MVTKLKKSRKMRGKNSSGHGRINKHRKNPGGKGKSGGQHHHKIIFERYRETYFRKTGMRI 60
Query: 260 FHFRKNKN 283
++ K N
Sbjct: 61 YNLEKTLN 68
>UniRef50_Q9LPV3 Cluster: Putative 60S ribosomal protein L27a-1;
n=1; Arabidopsis thaliana|Rep: Putative 60S ribosomal
protein L27a-1 - Arabidopsis thaliana (Mouse-ear cress)
Length = 104
Score = 46.4 bits (105), Expect = 0.001
Identities = 32/84 (38%), Positives = 41/84 (48%), Gaps = 2/84 (2%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGK--LGM 253
M TS+KKTR LR HVS H R + PG G +GM
Sbjct: 1 MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39
Query: 254 RNFHFRKNKNFCPVLNLDKLWTLV 325
R FH +NK +C ++NLDKLW++V
Sbjct: 40 RYFHKLRNKFYCQIVNLDKLWSMV 63
>UniRef50_P53143 Cluster: Putative uncharacterized protein YGL102C;
n=2; Saccharomycetaceae|Rep: Putative uncharacterized
protein YGL102C - Saccharomyces cerevisiae (Baker's
yeast)
Length = 142
Score = 44.4 bits (100), Expect = 0.004
Identities = 23/73 (31%), Positives = 37/73 (50%)
Frame = -3
Query: 349 ILQPRLFRD*CPKLI*VQNWTEILVLSKVEISHTKFAKVSRMVLVHVDXXXXXXXXXXXX 170
+L +F D CP+ + VQ+W + L VE+SHT +++ MV +HV+
Sbjct: 63 VLVSLVFWDQCPQFVQVQDWLPEMSLLLVEVSHTNLTEITWMVFIHVNSVVMLTTGHTST 122
Query: 169 XXXXXVLSDTTMS 131
VL+DTT +
Sbjct: 123 TGVLSVLTDTTFT 135
>UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S ribosomal
protein L27a; n=1; Rattus norvegicus|Rep: PREDICTED:
similar to 60S ribosomal protein L27a - Rattus
norvegicus
Length = 86
Score = 38.7 bits (86), Expect = 0.20
Identities = 21/51 (41%), Positives = 24/51 (47%)
Frame = +2
Query: 80 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPG 232
M+ +KTRK +GHVS NAGGEH HRIN PG
Sbjct: 1 MSGRLRKTRKFQGHVSDSHSRISKHHKHRGGQRNAGGEHLHRINCYYKVPG 51
>UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1;
Flavobacteriales bacterium HTCC2170|Rep: Putative
lipoprotein - Flavobacteriales bacterium HTCC2170
Length = 535
Score = 33.9 bits (74), Expect = 5.7
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -3
Query: 514 TQAPPTSLIFFSADFEKNFAFTMTGCLGSLPL 419
T A SL FFS D + N A T GCLG+ P+
Sbjct: 306 TDAEGNSLYFFSLDTKDNSACTSAGCLGAWPV 337
>UniRef50_Q6LXD1 Cluster: 50S ribosomal protein L15P; n=9;
Euryarchaeota|Rep: 50S ribosomal protein L15P -
Methanococcus maripaludis
Length = 143
Score = 33.9 bits (74), Expect = 5.7
Identities = 23/68 (33%), Positives = 35/68 (51%), Gaps = 3/68 (4%)
Frame = +2
Query: 179 NAGGEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASA 358
NAG + H +++ K++P YFGK G N+N C + L+ + E+ LKY A
Sbjct: 36 NAGHQKHKWLSVCKFNPDYFGKYGF-------NRNPCLIKQLETINIGELEEYILKYKDA 88
Query: 359 ---PDGKV 373
+GKV
Sbjct: 89 FQVEEGKV 96
>UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse
transcriptase; n=1; Arabidopsis thaliana|Rep: Putative
non-LTR retroelement reverse transcriptase - Arabidopsis
thaliana (Mouse-ear cress)
Length = 1044
Score = 33.1 bits (72), Expect = 9.9
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +1
Query: 214 GQVPSWILWQTWYEKFPL*KEQEFLSSFEL 303
GQ+P WILW W + L EQ+ +SS +L
Sbjct: 842 GQLPIWILWNLWNCRNKLIFEQKHISSMDL 871
>UniRef50_Q2H6Q7 Cluster: Putative uncharacterized protein; n=1;
Chaetomium globosum|Rep: Putative uncharacterized protein
- Chaetomium globosum (Soil fungus)
Length = 1511
Score = 33.1 bits (72), Expect = 9.9
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +2
Query: 272 KNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPAHQY 388
K K PV+ +D+LW V E + S PD +PA +Y
Sbjct: 948 KAKQKSPVIMVDQLWLWVLEDEKTVITSLPDTWIPAEKY 986
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 638,869,257
Number of Sequences: 1657284
Number of extensions: 10328298
Number of successful extensions: 21217
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 20565
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21201
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 81571813589
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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