BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP05_F_G03 (953 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 34 0.026 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 30 0.55 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.97 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.3 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 9.0 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 34.3 bits (75), Expect = 0.026 Identities = 26/89 (29%), Positives = 28/89 (31%), Gaps = 2/89 (2%) Frame = +1 Query: 508 PPKGGGVXGXXPPXKXXX--PPXXGPAVXXSPXPXHPXGXXXVPPXPRGXGPXPXXVXGG 681 PP G PP PP P++ S P P G PP P P G Sbjct: 404 PPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGM 463 Query: 682 VXXIXHPPXNPXXPPGKXFXXXXPAPXXP 768 PP P PP PAP P Sbjct: 464 PAAPPLPPAAPAPPPA-----PAPAPAAP 487 Score = 27.9 bits (59), Expect = 2.2 Identities = 27/114 (23%), Positives = 31/114 (27%), Gaps = 8/114 (7%) Frame = +3 Query: 630 PPXPPGX---GAXPXTXXRGGXXHPPPPXKPXXPXGXTFXXXXXG-----PXXPXNNX*X 785 PP PP G P PPPP P + G P P + Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPST 372 Query: 786 XRXXXPXXXXXXPPXPPQXKXXXXPPPXXGGXXPPXXXPXXXPXXSXSPXXPXP 947 R P PP PPP G P P + +P P P Sbjct: 373 GRQPPPLSSSRAVSNPPA------PPPAIPGRSAPALPPLGNASRTSTPPVPTP 420 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 29.9 bits (64), Expect = 0.55 Identities = 20/54 (37%), Positives = 20/54 (37%) Frame = +1 Query: 562 PPXXGPAVXXSPXPXHPXGXXXVPPXPRGXGPXPXXVXGGVXXIXHPPXNPXXP 723 PP PAV P P P PP P GP P GV PP P P Sbjct: 732 PPPPPPAVIV-PTPA-PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.1 bits (62), Expect = 0.97 Identities = 25/101 (24%), Positives = 29/101 (28%), Gaps = 2/101 (1%) Frame = +1 Query: 541 PPXKXXXPPXXGPAVXXSPXPXHPXGXXXVPPXPRGXGPXPXXVXGGVXXIXHPPXNPXX 720 P PP P+ P P G VPP P P P GV + P P Sbjct: 1162 PKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP-PSEAPPVPKPSV-GVPPVPPPSTAPPV 1219 Query: 721 PPGKXFXXXXPAP--XXP*ITIXXPAXGXLXXPXXXPPXPH 837 P P P P + + P P PH Sbjct: 1220 PTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTPRSSVPSPH 1260 Score = 26.6 bits (56), Expect = 5.1 Identities = 27/122 (22%), Positives = 32/122 (26%), Gaps = 4/122 (3%) Frame = +3 Query: 594 PXSSSPPXX--GXXPPXPPGXGAXPXTXXRGGXXHPPPPXKPXXPXGXTFXXXXXGPXXP 767 P + +PP PP P P G PPP + P P Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVP 1086 Query: 768 XNNX*XXRXXXPXXXXXXPPXP-PQXKXXXXPPPXXGGXXP-PXXXPXXXPXXSXSPXXP 941 + P PP P P P P P P P S +P P Sbjct: 1087 APS---GIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVP 1143 Query: 942 XP 947 P Sbjct: 1144 KP 1145 Score = 26.2 bits (55), Expect = 6.8 Identities = 13/40 (32%), Positives = 14/40 (35%) Frame = +1 Query: 544 PXKXXXPPXXGPAVXXSPXPXHPXGXXXVPPXPRGXGPXP 663 P PP P+V P P G VP G P P Sbjct: 1153 PAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVP 1192 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 28.7 bits (61), Expect = 1.3 Identities = 15/52 (28%), Positives = 16/52 (30%) Frame = +1 Query: 508 PPKGGGVXGXXPPXKXXXPPXXGPAVXXSPXPXHPXGXXXVPPXPRGXGPXP 663 P + PP PP PA P P VPP P P P Sbjct: 154 PASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.8 bits (54), Expect = 9.0 Identities = 15/43 (34%), Positives = 16/43 (37%) Frame = +3 Query: 822 PPXPPQXKXXXXPPPXXGGXXPPXXXPXXXPXXSXSPXXPXPG 950 PP P PP G PP P P S +P P PG Sbjct: 1708 PPPPMSVPPPPSAPPMPAG--PPSAPPPPLPASS-APSVPNPG 1747 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,582,121 Number of Sequences: 5004 Number of extensions: 17866 Number of successful extensions: 70 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 25 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 48 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 487313384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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