BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP05_F_E11 (867 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces p... 54 3e-08 SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizos... 27 4.6 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 27 4.6 SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 26 6.0 >SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 54.0 bits (124), Expect = 3e-08 Identities = 28/63 (44%), Positives = 39/63 (61%), Gaps = 2/63 (3%) Frame = +1 Query: 640 QVQYXLVFCLWVLTFNPPIG*KMNK-FNVIPILADILSDSVKEKVPRIVLXVFXNLIEK- 813 Q+QY +FC W LTF I +NK F++I +L I+ K KV R+VL + NLI+K Sbjct: 221 QLQYYSLFCFWQLTFESHIAQDINKRFDLIKLLVQIIRSDTKTKVYRLVLAILVNLIDKA 280 Query: 814 PED 822 P+D Sbjct: 281 PKD 283 Score = 27.9 bits (59), Expect = 2.0 Identities = 20/82 (24%), Positives = 38/82 (46%), Gaps = 4/82 (4%) Frame = +2 Query: 68 SEIRQTQINWQSYLQSQMITQRDHDFIVNLDQR---GQKDLPDKNPDACAEVFLNLLTHI 238 + +R I WQ Y +S + + + I NL + +++ A + +FL LL+ Sbjct: 29 NNVRCVAIPWQGYQRSGSLEENELQEIENLTGKPLSAYVKTAEEDTTAYSNLFLKLLSMK 88 Query: 239 SKDHTIQYILV-LIDDILSEDK 301 + + LV L D +L+ +K Sbjct: 89 DTPDVVNFALVKLADTLLNSNK 110 >SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 891 Score = 26.6 bits (56), Expect = 4.6 Identities = 12/44 (27%), Positives = 22/44 (50%) Frame = -3 Query: 649 IEPGSSLWRPRIDSKVDIPSTERKAKRYSSIRSIICRHRATDWI 518 + P S+L K ST + +RYS+ ++ H A++W+ Sbjct: 561 VNPWSTLELSLSMKKALTLSTNERNQRYSNCLDVVLTHSASNWV 604 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 26.6 bits (56), Expect = 4.6 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = -3 Query: 322 TKYLHPRFIFRKNVINEHKNVLNGVVLADMRQEVEKDFGTGIGIL 188 +KY P+F+F K ++E + + +G+ + M V + G I +L Sbjct: 46 SKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDML 90 >SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr 1|||Manual Length = 683 Score = 26.2 bits (55), Expect = 6.0 Identities = 17/55 (30%), Positives = 28/55 (50%) Frame = +2 Query: 287 LSEDKSRVKIFRETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLACWHPQLMD 451 L DK+++K+F E K ++ P N++ D F +I +L C H LM+ Sbjct: 76 LQNDKTKLKLFGEIKVHQSMSHP--NIVGFIDCFEDSTNIYLILEL-CEHKSLME 127 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,399,363 Number of Sequences: 5004 Number of extensions: 67972 Number of successful extensions: 197 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 197 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 432473040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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