BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP05_F_D15 (932 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 27 3.8 SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 27 5.0 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 26 6.6 SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy... 26 8.7 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 27.1 bits (57), Expect = 3.8 Identities = 9/29 (31%), Positives = 18/29 (62%) Frame = +2 Query: 626 SVGMVSVKKTYHTKDVIVRPKSEIEIHGY 712 ++G++S K +HT D+I+ ++ H Y Sbjct: 402 NIGVISGIKQFHTGDIIINKENSKNFHEY 430 >SPBC25H2.03 |||vacuolar protein involved in phosphoinositide metabolism|Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 26.6 bits (56), Expect = 5.0 Identities = 13/34 (38%), Positives = 17/34 (50%) Frame = +2 Query: 74 KSQNIFKVVLI*WCRNPVEAHKVVFEKCILQK*Y 175 K QNIF + WC N + VF C+L + Y Sbjct: 598 KLQNIFTTLYTAWCHNSI----AVFSLCLLSQNY 627 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.2 bits (55), Expect = 6.6 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = -1 Query: 266 ANSLTHTFKLFKKVPSGSTAHNHLCK*WRQNTI 168 +NSLTH F + +T + +CK W+ + I Sbjct: 202 SNSLTHEKIEFATITKDTTKNKMVCKIWKSDEI 234 >SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1019 Score = 25.8 bits (54), Expect = 8.7 Identities = 13/48 (27%), Positives = 25/48 (52%) Frame = +1 Query: 250 WVREFAIRSSALAQQSTYSKRLKD*STVLNPRKTNKIR*SVVVEQSEI 393 ++R F RSS ++ S K ++D R+T + + V+EQ ++ Sbjct: 605 FIRSFTFRSSNNSRMSQVFKDIQDMKKAATKRETERKEFADVIEQDKL 652 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,351,288 Number of Sequences: 5004 Number of extensions: 64071 Number of successful extensions: 134 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 134 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 473333082 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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