Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_B21
(914 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family... 33 0.26
At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid t... 33 0.26
At3g06750.1 68416.m00800 hydroxyproline-rich glycoprotein family... 33 0.35
At5g49280.1 68418.m06099 hydroxyproline-rich glycoprotein family... 32 0.46
At1g61080.1 68414.m06877 proline-rich family protein 29 4.3
At1g59910.1 68414.m06749 formin homology 2 domain-containing pro... 27 4.7
At3g08530.1 68416.m00990 clathrin heavy chain, putative similar ... 29 5.7
At1g53260.1 68414.m06035 hypothetical protein low similarity to ... 29 5.7
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 25 7.9
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 25 7.9
At1g31810.1 68414.m03904 formin homology 2 domain-containing pro... 28 9.9
>At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family
protein Common family members: At4g18570, At4g04980,
At5g61090 [Arabidopsis thaliana]; identical to cDNA
CHUP1 for actin binding protein GI:28071264
Length = 1004
Score = 33.1 bits (72), Expect = 0.26
Identities = 14/22 (63%), Positives = 14/22 (63%)
Frame = +1
Query: 709 GGGXPPPPPXXGGGXXFPXXPP 774
GGG PPPPP GGG P PP
Sbjct: 676 GGGPPPPPPPPGGG---PPPPP 694
Score = 29.9 bits (64), Expect = 2.5
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +1
Query: 712 GGXPPPPPXXGGGXXFPXXPP 774
GG PPPPP GGG P PP
Sbjct: 687 GGGPPPPP--GGGPPPPPPPP 705
Score = 27.9 bits (59), Expect = 9.9
Identities = 13/35 (37%), Positives = 13/35 (37%)
Frame = -1
Query: 470 GGGGXKXXPPPRXXXKXXXXXPPPRXGXGGAPXPP 366
GGG P R PPP GG P PP
Sbjct: 660 GGGKSTNLPSARPPLPGGGPPPPPPPPGGGPPPPP 694
>At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to auxin
down regulated GB:X69640 GI:296442 from [Glycine max];
contains Pfam profile PF00234: Protease inhibitor/seed
storage/LTP family
Length = 297
Score = 33.1 bits (72), Expect = 0.26
Identities = 17/34 (50%), Positives = 17/34 (50%)
Frame = -1
Query: 476 GXGGGGXKXXPPPRXXXKXXXXXPPPRXGXGGAP 375
G GGGG K PPP K PPP G GG P
Sbjct: 45 GGGGGGSK--PPPHHGGKGGGK-PPPHGGKGGGP 75
Score = 32.3 bits (70), Expect = 0.46
Identities = 14/23 (60%), Positives = 14/23 (60%), Gaps = 1/23 (4%)
Frame = -1
Query: 773 GGXXGKXXPPPXXGG-GGGXPPP 708
GG G PPP GG GGG PPP
Sbjct: 45 GGGGGGSKPPPHHGGKGGGKPPP 67
Score = 31.1 bits (67), Expect = 1.1
Identities = 14/32 (43%), Positives = 14/32 (43%)
Frame = -3
Query: 471 GGGGXKXXTPPPXXXKXXXXFXPPXXGGGGGP 376
GGGG PPP PP G GGGP
Sbjct: 44 GGGGGGGSKPPPHHGGKGGGKPPPHGGKGGGP 75
Score = 27.9 bits (59), Expect = 9.9
Identities = 17/48 (35%), Positives = 18/48 (37%)
Frame = -2
Query: 523 KKXKXGGGGXXFXXXXXXGGGXKXXXPPPAXXKXXXXFXPPXXGGXGG 380
K + GGGG GG PPP K PP GG GG
Sbjct: 39 KPPQHGGGGGGGSKPPPHHGGKGGGKPPPHGGKGG---GPPHHGGGGG 83
>At3g06750.1 68416.m00800 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 147
Score = 32.7 bits (71), Expect = 0.35
Identities = 15/39 (38%), Positives = 18/39 (46%), Gaps = 1/39 (2%)
Frame = -3
Query: 471 GGGGXKXXTPPPXXXKXXXXFXPPXXGGG-GGPXPPXXF 358
GGGG +PPP + PP G G GG PP +
Sbjct: 60 GGGGSYYYSPPPPSSSGGVKYPPPYGGDGYGGYYPPPYY 98
Score = 27.9 bits (59), Expect = 9.9
Identities = 13/47 (27%), Positives = 15/47 (31%)
Frame = -2
Query: 508 GGGGXXFXXXXXXGGGXKXXXPPPAXXKXXXXFXPPXXGGXGGPPXP 368
GGG + G PP + PP G G PP P
Sbjct: 61 GGGSYYYSPPPPSSSGGVKYPPPYGGDGYGGYYPPPYYGNYGTPPPP 107
Score = 27.9 bits (59), Expect = 9.9
Identities = 14/33 (42%), Positives = 14/33 (42%)
Frame = -1
Query: 464 GGXKXXPPPRXXXKXXXXXPPPRXGXGGAPXPP 366
GG K PPP PPP G G P PP
Sbjct: 76 GGVKY-PPPYGGDGYGGYYPPPYYGNYGTPPPP 107
>At5g49280.1 68418.m06099 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 162
Score = 32.3 bits (70), Expect = 0.46
Identities = 17/61 (27%), Positives = 20/61 (32%)
Frame = +3
Query: 390 PPXXGGXXXXXFXXXAGGGXXFXXPPPXXXXXXXXXPPPPXXFFFFFXFXXXRGGGGGGX 569
PP G GGG + PPP PP P +F F + G G
Sbjct: 84 PPSQSGGGSKYPPPYGGGGQGYYYPPPYSGNYPTPPPPNPIVPYFPFYYHTPPPGSGSDR 143
Query: 570 F 572
F
Sbjct: 144 F 144
Score = 29.1 bits (62), Expect = 4.3
Identities = 13/37 (35%), Positives = 15/37 (40%)
Frame = -3
Query: 468 GGGXKXXTPPPXXXKXXXXFXPPXXGGGGGPXPPXXF 358
GGG PPP + PP GGG G P +
Sbjct: 75 GGGSSYYYPPPSQSGGGSKYPPPYGGGGQGYYYPPPY 111
Score = 28.3 bits (60), Expect = 7.5
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +1
Query: 709 GGGXPPPPPXXGGGXXFPXXPP 774
GGG PPP GGG + PP
Sbjct: 89 GGGSKYPPPYGGGGQGYYYPPP 110
Score = 27.9 bits (59), Expect = 9.9
Identities = 13/47 (27%), Positives = 15/47 (31%)
Frame = -2
Query: 508 GGGGXXFXXXXXXGGGXKXXXPPPAXXKXXXXFXPPXXGGXGGPPXP 368
GGG + GG PP + PP G PP P
Sbjct: 75 GGGSSYYYPPPSQSGGGSKYPPPYGGGGQGYYYPPPYSGNYPTPPPP 121
>At1g61080.1 68414.m06877 proline-rich family protein
Length = 907
Score = 29.1 bits (62), Expect = 4.3
Identities = 19/71 (26%), Positives = 20/71 (28%), Gaps = 4/71 (5%)
Frame = -1
Query: 566 PPPXPPPXXXXXXXXXXXXXXXXXXXXXXXGXGGGGXKXXPPPRXXXKXXXXXPPP---- 399
PPP PPP G PPP + PPP
Sbjct: 524 PPPPPPPRAAVAPPPPPPPPGTAAAPPPPPPPPGTQAAPPPPPPPPMQNRAPSPPPMPMG 583
Query: 398 RXGXGGAPXPP 366
G GG P PP
Sbjct: 584 NSGSGGPPPPP 594
Score = 28.7 bits (61), Expect = 5.7
Identities = 16/64 (25%), Positives = 17/64 (26%)
Frame = +1
Query: 721 PPPPPXXGGGXXFPXXPPXXRXXXXXKXXXXXXXXXXXXXXXXXPPXPXXXAGXLXFLSX 900
PPP P G P PP PP P G +
Sbjct: 577 PPPMPMGNSGSGGPPPPPPPMPLANGATPPPPPPPMAMANGAAGPPPPPPRMGMANGAAG 636
Query: 901 PPPP 912
PPPP
Sbjct: 637 PPPP 640
>At1g59910.1 68414.m06749 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02128
Length = 929
Score = 26.6 bits (56), Expect(2) = 4.7
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = -1
Query: 446 PPPRXXXKXXXXXPPPRXGXGGAPXPP 366
PPP K PPP G P PP
Sbjct: 410 PPPPPGKKGAGPPPPPPMSKKGPPKPP 436
Score = 25.8 bits (54), Expect(2) = 7.9
Identities = 10/27 (37%), Positives = 11/27 (40%)
Frame = -1
Query: 446 PPPRXXXKXXXXXPPPRXGXGGAPXPP 366
PPP+ PPP G P PP
Sbjct: 399 PPPKKGPAAPPPPPPPGKKGAGPPPPP 425
Score = 20.6 bits (41), Expect(2) = 7.9
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = -1
Query: 566 PPPXPPP 546
PPP PPP
Sbjct: 395 PPPPPPP 401
Score = 20.6 bits (41), Expect(2) = 4.7
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = -1
Query: 566 PPPXPPP 546
PPP PPP
Sbjct: 408 PPPPPPP 414
>At3g08530.1 68416.m00990 clathrin heavy chain, putative similar to
Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo
sapiens]
Length = 1703
Score = 28.7 bits (61), Expect = 5.7
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +1
Query: 700 GXXGGGXPPPPPXXGGGXXFPXXPP 774
G GGG PPP GG P PP
Sbjct: 1670 GMGGGGGYGPPPQMGGMPGMPPMPP 1694
>At1g53260.1 68414.m06035 hypothetical protein low similarity to
SP|Q38732 DAG protein, chloroplast precursor
{Antirrhinum majus}
Length = 358
Score = 28.7 bits (61), Expect = 5.7
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1
Query: 407 PPPRXGXGGAPXPP 366
PPP G GGAP PP
Sbjct: 193 PPPNQGMGGAPPPP 206
>At5g54650.2 68418.m06805 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 25.4 bits (53), Expect(2) = 7.9
Identities = 14/35 (40%), Positives = 14/35 (40%)
Frame = -1
Query: 470 GGGGXKXXPPPRXXXKXXXXXPPPRXGXGGAPXPP 366
G GG K PPP PPP AP PP
Sbjct: 396 GSGGPKPPPPP---GPKGPRPPPPMSLGPKAPRPP 427
Score = 21.0 bits (42), Expect(2) = 7.9
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = -1
Query: 572 KXPPPXPPP 546
+ PPP PPP
Sbjct: 387 RPPPPAPPP 395
>At5g54650.1 68418.m06804 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 25.4 bits (53), Expect(2) = 7.9
Identities = 14/35 (40%), Positives = 14/35 (40%)
Frame = -1
Query: 470 GGGGXKXXPPPRXXXKXXXXXPPPRXGXGGAPXPP 366
G GG K PPP PPP AP PP
Sbjct: 396 GSGGPKPPPPP---GPKGPRPPPPMSLGPKAPRPP 427
Score = 21.0 bits (42), Expect(2) = 7.9
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = -1
Query: 572 KXPPPXPPP 546
+ PPP PPP
Sbjct: 387 RPPPPAPPP 395
>At1g31810.1 68414.m03904 formin homology 2 domain-containing
protein / FH2 domain-containing protein low similarity
to SP|P48608 Diaphanous protein {Drosophila
melanogaster}; contains Pfam profile PF02181: Formin
Homology 2(FH2) Domain
Length = 1201
Score = 27.9 bits (59), Expect = 9.9
Identities = 18/63 (28%), Positives = 18/63 (28%)
Frame = -1
Query: 566 PPPXPPPXXXXXXXXXXXXXXXXXXXXXXXGXGGGGXKXXPPPRXXXKXXXXXPPPRXGX 387
PPP PPP G G G PPP PPP G
Sbjct: 714 PPPPPPPPLSKTPAPPPPPLSKTPVPPPPPGLGRG-TSSGPPPLGAKGSNAPPPPPPAGR 772
Query: 386 GGA 378
G A
Sbjct: 773 GRA 775
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,692,835
Number of Sequences: 28952
Number of extensions: 283550
Number of successful extensions: 2423
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 494
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1992
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2168774904
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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