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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP05_F_B18
         (996 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom...    38   0.008
At3g08790.1 68416.m01021 VHS domain-containing protein / GAT dom...    37   0.024
At5g01760.1 68418.m00095 VHS domain-containing protein / GAT dom...    36   0.055
At5g16880.3 68418.m01977 VHS domain-containing protein / GAT dom...    34   0.17 
At5g16880.2 68418.m01979 VHS domain-containing protein / GAT dom...    34   0.17 
At5g16880.1 68418.m01978 VHS domain-containing protein / GAT dom...    34   0.17 
At4g32760.1 68417.m04661 VHS domain-containing protein / GAT dom...    33   0.22 
At1g21380.1 68414.m02675 VHS domain-containing protein / GAT dom...    33   0.30 
At1g76970.1 68414.m08962 VHS domain-containing protein / GAT dom...    32   0.68 
At5g22440.1 68418.m02617 60S ribosomal protein L10A (RPL10aC)          29   6.4  
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila...    29   6.4  

>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to hepatocyte
           growth factor-regulated tyrosine kinase substrate HRS
           isoform 2 [Homo sapiens] GI:9022389; contains Pfam
           profiles PF00790: VHS domain, PF03127: GAT domain
          Length = 671

 Score = 38.3 bits (85), Expect = 0.008
 Identities = 16/35 (45%), Positives = 22/35 (62%)
 Frame = +1

Query: 370 MYTLTVLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           +  LT+LET VKNCG   H  V  K  + E+VK++
Sbjct: 62  LLALTLLETLVKNCGDYLHHQVAEKNILGEMVKIV 96


>At3g08790.1 68416.m01021 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to
           HGF-regulated tyrosine kinase substrate [Mus musculus]
           GI:1089781; contains Pfam profiles PF00790: VHS domain,
           PF03127: GAT domain
          Length = 607

 Score = 36.7 bits (81), Expect = 0.024
 Identities = 19/53 (35%), Positives = 30/53 (56%)
 Frame = +1

Query: 313 SNKKKGLTTSAGKETTQW*MYTLTVLETCVKNCGKPFHVLVCNKEFISELVKL 471
           S  KK LT+   K      +  LT+LET + NCG+  H+ V  K+ + ++VK+
Sbjct: 41  SGIKKRLTSRTSKVQ----LLALTLLETIITNCGELIHMQVAEKDILHKMVKM 89


>At5g01760.1 68418.m00095 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to Hrs [Rattus
           norvegicus] GI:8547026; contains Pfam profiles PF00790:
           VHS domain, PF03127: GAT domain
          Length = 542

 Score = 35.5 bits (78), Expect = 0.055
 Identities = 14/35 (40%), Positives = 22/35 (62%)
 Frame = +1

Query: 370 MYTLTVLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           + TLT+LE  +KNCG   H  +  K  + ++VKL+
Sbjct: 76  LLTLTLLEAMLKNCGDFVHSHIAEKHLLEDMVKLV 110


>At5g16880.3 68418.m01977 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to hepatocyte
           growth factor-regulated tyrosine kinase substrate [Homo
           sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
           domain, PF03127: GAT domain
          Length = 297

 Score = 33.9 bits (74), Expect = 0.17
 Identities = 17/32 (53%), Positives = 21/32 (65%)
 Frame = +1

Query: 379 LTVLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           L +LETCVKNC K F   V  +  + E+VKLI
Sbjct: 105 LVLLETCVKNCEKAFSE-VAAERVLDEMVKLI 135


>At5g16880.2 68418.m01979 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to hepatocyte
           growth factor-regulated tyrosine kinase substrate [Homo
           sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
           domain, PF03127: GAT domain
          Length = 407

 Score = 33.9 bits (74), Expect = 0.17
 Identities = 17/32 (53%), Positives = 21/32 (65%)
 Frame = +1

Query: 379 LTVLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           L +LETCVKNC K F   V  +  + E+VKLI
Sbjct: 105 LVLLETCVKNCEKAFSE-VAAERVLDEMVKLI 135


>At5g16880.1 68418.m01978 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to hepatocyte
           growth factor-regulated tyrosine kinase substrate [Homo
           sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
           domain, PF03127: GAT domain
          Length = 407

 Score = 33.9 bits (74), Expect = 0.17
 Identities = 17/32 (53%), Positives = 21/32 (65%)
 Frame = +1

Query: 379 LTVLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           L +LETCVKNC K F   V  +  + E+VKLI
Sbjct: 105 LVLLETCVKNCEKAFSE-VAAERVLDEMVKLI 135


>At4g32760.1 68417.m04661 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to hepatocyte
           growth factor-regulated tyrosine kinase substrate HRS
           isoform 2 [Homo sapiens] GI:9022389; contains Pfam
           profiles PF00790: VHS domain, PF03127: GAT domain
          Length = 838

 Score = 33.5 bits (73), Expect = 0.22
 Identities = 14/30 (46%), Positives = 20/30 (66%)
 Frame = +1

Query: 385 VLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           +LET VKNCG   H+ V  K  I E+V+++
Sbjct: 77  LLETIVKNCGDMVHMHVAEKGVIHEMVRIV 106


>At1g21380.1 68414.m02675 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to Hrs [Rattus
           norvegicus] GI:8547026; contains Pfam profiles PF00790:
           VHS domain, PF03127: GAT domain
          Length = 506

 Score = 33.1 bits (72), Expect = 0.30
 Identities = 14/50 (28%), Positives = 29/50 (58%)
 Frame = +1

Query: 325 KGLTTSAGKETTQW*MYTLTVLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           K L    G + ++  +  L  LET  KNCG+  + L+ +++ + ++VK++
Sbjct: 44  KVLKKRLGSKNSKVQILALYALETLSKNCGESVYQLIVDRDILPDMVKIV 93


>At1g76970.1 68414.m08962 VHS domain-containing protein / GAT
           domain-containing protein weak similarity to
           HGF-regulated tyrosine kinase substrate [Mus musculus]
           GI:1089781; contains Pfam profiles PF00790: VHS domain,
           PF03127: GAT domain
          Length = 446

 Score = 31.9 bits (69), Expect = 0.68
 Identities = 14/50 (28%), Positives = 29/50 (58%)
 Frame = +1

Query: 325 KGLTTSAGKETTQW*MYTLTVLETCVKNCGKPFHVLVCNKEFISELVKLI 474
           K L    G + ++  +  L  LET  KNCG+  + L+ ++  ++++VK++
Sbjct: 44  KVLKKRLGSKNSKVQILALYALETLSKNCGENVYQLIIDRGLLNDMVKIV 93


>At5g22440.1 68418.m02617 60S ribosomal protein L10A (RPL10aC)
          Length = 217

 Score = 28.7 bits (61), Expect = 6.4
 Identities = 10/28 (35%), Positives = 17/28 (60%)
 Frame = +1

Query: 400 VKNCGKPFHVLVCNKEFISELVKLIGPG 483
           VK   K FH  + ++  I ++ +L+GPG
Sbjct: 100 VKKLAKKFHAFLASESVIKQIPRLLGPG 127


>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar to
            CAF protein [Arabidopsis thaliana] GI:6102610; contains
            Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
            Helicase conserved C-terminal domain, PF03368: Domain of
            unknown function, PF00636: RNase3 domain, PF00035:
            Double-stranded RNA binding motif
          Length = 1676

 Score = 28.7 bits (61), Expect = 6.4
 Identities = 11/19 (57%), Positives = 12/19 (63%)
 Frame = +1

Query: 391  ETCVKNCGKPFHVLVCNKE 447
            ETCV NC KP H   C +E
Sbjct: 1602 ETCVANCWKPPHFECCEEE 1620


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,367,222
Number of Sequences: 28952
Number of extensions: 164028
Number of successful extensions: 368
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 368
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2431362000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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