Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_B06
(934 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 161 2e-40
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 146 5e-36
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 104 2e-23
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 74 3e-14
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 61 3e-10
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 47 4e-06
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 35 0.019
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 2.2
SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 2.2
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 27 2.9
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 6.6
SPBC839.03c |||neddylation protein Dcn1|Schizosaccharomyces pomb... 26 6.6
SPAC9E9.17c |||dubiuos|Schizosaccharomyces pombe|chr 1|||Manual 26 8.7
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 161 bits (390), Expect = 2e-40
Identities = 84/199 (42%), Positives = 112/199 (56%)
Frame = +2
Query: 155 IPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTRED 334
IP GLGTW+S+P + AVK A+ GYRHID A +YGNE EVG+ I KE V R+D
Sbjct: 24 IPGLGLGTWRSEPNQTKNAVKTALQYGYRHIDAAAIYGNEDEVGDGI----KESGVPRKD 79
Query: 335 LFITSKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIHWPQAYKEDGSLFPTDQSGKIQ 514
+++TSKLW H P+ V AL++TL +L + YLD YLIHWP ++K FP D+ G +
Sbjct: 80 IWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKFPKDKDGNLI 139
Query: 515 FXXXXXXXXXXXXXXXXXKRVWSRASESPTSNSKQLERLLQHATVKPVVNQVECHPYLNQ 694
+ S N LER+L+ A VKP V+Q+E HP+L Q
Sbjct: 140 YEKNPIEETWKAMEKLLETGKVRHIGLS-NFNDTNLERILKVAKVKPAVHQMELHPFLPQ 198
Query: 695 KRLKEFCEARGVKITAYSP 751
E + G+ +TAYSP
Sbjct: 199 TEFVEKHKKLGIHVTAYSP 217
Score = 28.3 bits (60), Expect = 1.6
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +1
Query: 532 TWTPGKAMEPLVKEGLVKSIGVSNF 606
TW KAME L++ G V+ IG+SNF
Sbjct: 148 TW---KAMEKLLETGKVRHIGLSNF 169
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 146 bits (353), Expect = 5e-36
Identities = 79/199 (39%), Positives = 112/199 (56%)
Frame = +2
Query: 155 IPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTRED 334
IP GLGTW+S E AV A+ GYRHID A++YGNEKE+GE I +E V R D
Sbjct: 23 IPSIGLGTWRSGKDETKNAVCAALKAGYRHIDTAHIYGNEKEIGEGI----RESGVPRTD 78
Query: 335 LFITSKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIHWPQAYKEDGSLFPTDQSGKIQ 514
+++TSKLW HR LV AL++TL +LN++Y+D YLIHWP A P ++ G++
Sbjct: 79 IWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEELPRNEKGELI 138
Query: 515 FXXXXXXXXXXXXXXXXXKRVWSRASESPTSNSKQLERLLQHATVKPVVNQVECHPYLNQ 694
+ + R N++ L+R+L+ A VKP ++Q+E HPYL Q
Sbjct: 139 Y-EDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKPTIHQMELHPYLPQ 197
Query: 695 KRLKEFCEARGVKITAYSP 751
E + + ++AYSP
Sbjct: 198 TEYLEKHKKLQIHVSAYSP 216
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 104 bits (249), Expect = 2e-23
Identities = 76/207 (36%), Positives = 104/207 (50%), Gaps = 8/207 (3%)
Frame = +2
Query: 155 IPVFGLGT--WKSKPGEVTQ----AVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEG 316
+P +G+GT +K + GE+ + +VK+A+ G+ HIDCA VYGNE+EVG A +KE
Sbjct: 12 VPAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAEVYGNEEEVGVA----LKEA 67
Query: 317 VVTREDLFITSKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIHWPQAYKEDGSLFPTD 496
V R LFITSK+ H D + AL E+L L YLDLYL+H P + E P
Sbjct: 68 NVPRSKLFITSKV---MHNVDNIPEALNESLRKLGTDYLDLYLLHSPIPFYE--KKIPIS 122
Query: 497 QSGKIQFXXXXXXXXXXXXXXXXXKRVWSRASESPTSNSKQLERLLQHATVKPVVNQVEC 676
+ K V P LE LL+ +T+ P VNQ+E
Sbjct: 123 EGWKAM---------ETALGTGLVHSVGVSNFRIP-----DLEELLKTSTITPRVNQIEF 168
Query: 677 HP--YLNQKRLKEFCEARGVKITAYSP 751
HP Y K L EFC+++G+ + Y P
Sbjct: 169 HPQVYKAAKPLVEFCQSKGIIVEGYGP 195
Score = 30.3 bits (65), Expect = 0.41
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +1
Query: 547 KAMEPLVKEGLVKSIGVSNFKL 612
KAME + GLV S+GVSNF++
Sbjct: 126 KAMETALGTGLVHSVGVSNFRI 147
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 73.7 bits (173), Expect = 3e-14
Identities = 44/107 (41%), Positives = 59/107 (55%)
Frame = +2
Query: 152 LIPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTRE 331
+IP G G + K E V A+D GYRHID A VYGNE G+AI ++ V R
Sbjct: 18 VIPRIGFGAFMLKYNECYGLVTQALDSGYRHIDTAAVYGNEDICGKAIVDWCEKNNVKRT 77
Query: 332 DLFITSKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIHWPQAYKE 472
D+F+TSKL N R A++ +L +L Y+DL+LI P K+
Sbjct: 78 DIFLTSKLANCSDYYS-TRAAIRSSLHHLGT-YIDLFLIQSPAGGKK 122
Score = 42.3 bits (95), Expect = 9e-05
Identities = 15/33 (45%), Positives = 24/33 (72%)
Frame = +2
Query: 653 PVVNQVECHPYLNQKRLKEFCEARGVKITAYSP 751
P VNQ+E HP+L+Q + ++C++ + I AYSP
Sbjct: 165 PCVNQIELHPFLSQDDIVKYCQSHDIAIEAYSP 197
Score = 29.1 bits (62), Expect = 0.94
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +1
Query: 547 KAMEPLVKEGLVKSIGVSNFKLEAAGEVAAA 639
KAME V G ++S+GVSN+ ++ E+ A+
Sbjct: 129 KAMEEFVDSGDIRSVGVSNYGVKHLQELYAS 159
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 60.9 bits (141), Expect = 3e-10
Identities = 33/100 (33%), Positives = 52/100 (52%)
Frame = +2
Query: 158 PVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTREDL 337
P F G++ + +V A+ GYRHID A +Y NE + G AI ++E RED+
Sbjct: 15 PQFAYGSYMVNRTKCFDSVYAALQCGYRHIDSAQMYHNEADCGRAILKFMEETGTKREDI 74
Query: 338 FITSKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIHWP 457
+ TSKL N ++ ++ + Y+DL+L+H P
Sbjct: 75 WFTSKL-NDLSGYKSTLSSIDASVKACGLGYIDLFLLHSP 113
Score = 50.4 bits (115), Expect = 4e-07
Identities = 17/43 (39%), Positives = 32/43 (74%)
Frame = +2
Query: 623 ERLLQHATVKPVVNQVECHPYLNQKRLKEFCEARGVKITAYSP 751
E L H + P VNQ+E HP+ +Q+++ ++CE++G+++ AY+P
Sbjct: 148 ELLDSHPKIIPCVNQIELHPFCSQQKVVDYCESKGIQLAAYAP 190
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/20 (60%), Positives = 17/20 (85%)
Frame = +1
Query: 547 KAMEPLVKEGLVKSIGVSNF 606
KA+E V+EG +++IGVSNF
Sbjct: 122 KALEKGVEEGKLRAIGVSNF 141
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 46.8 bits (106), Expect = 4e-06
Identities = 28/86 (32%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +2
Query: 206 QAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGV-VTREDLFITSKLWNTFHRPDL 382
Q V +A+ +G R ID A Y NEKE +AI + V + RED+ + +K+ ++ +
Sbjct: 28 QQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITKIPDSLQGFER 87
Query: 383 VRGALQETLDNLNVQYLDLYLIHWPQ 460
A++++L LD+ LIH P+
Sbjct: 88 TWKAVEQSLRRTGRPKLDVVLIHSPK 113
Score = 32.7 bits (71), Expect = 0.076
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +2
Query: 608 NSKQLERLLQHATVKPVVNQVECHPYLNQKRLKEFCEARGVKITAYSP 751
N LE ++ P +NQVE + N+ +C G+ + A+SP
Sbjct: 143 NIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYCFNHGILVQAFSP 190
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 34.7 bits (76), Expect = 0.019
Identities = 34/116 (29%), Positives = 51/116 (43%), Gaps = 15/116 (12%)
Frame = +2
Query: 155 IPVFGLGTWKSKPGE------VTQAVKDAIDIGYRHIDCAYVYGN---EKEVGEAITS-- 301
+ F LG W + E +K A D+G D A +Y N E +G+AI
Sbjct: 25 VSAFSLGGWLTYGNEGYDVEHTKNCLKQAWDLGINTFDTAEIYSNGNSETVMGKAIKELG 84
Query: 302 -KIKEGVVTREDLF-ITSKLWNT--FHRPDLVRGALQETLDNLNVQYLDLYLIHWP 457
E V+T + F +KL NT R ++ G L +L L + Y+D+ + H P
Sbjct: 85 WDRSEYVITTKVFFGAGTKLPNTTGLSRKHIIEG-LNASLKRLGLPYVDVIMAHRP 139
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 27.9 bits (59), Expect = 2.2
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 5/42 (11%)
Frame = +2
Query: 341 ITSKLWNTFHRPDLVRGALQET----LDNLNV-QYLDLYLIH 451
+ S+ W T H+ L RGAL E +DN V +Y+ Y I+
Sbjct: 640 VISRTWKTAHKNKLQRGALPEDEGSGVDNFRVKRYVSKYTIN 681
>SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 390
Score = 27.9 bits (59), Expect = 2.2
Identities = 10/39 (25%), Positives = 20/39 (51%)
Frame = +2
Query: 338 FITSKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIHW 454
+ + + + R +L++ L E L + LD+Y +HW
Sbjct: 123 YFSKEYLTAYRRSELLQNKLHEYQKKLITRVLDMYQLHW 161
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 27.5 bits (58), Expect = 2.9
Identities = 30/95 (31%), Positives = 46/95 (48%), Gaps = 10/95 (10%)
Frame = +2
Query: 197 EVTQAV-KDAIDIGYRHIDCAYVYG---NEKEVGEAI--TSKIKE-GVVTR---EDLFIT 346
E QAV A D+G D + +YG NE+ +G T + KE + T+ E T
Sbjct: 33 EANQAVLTHAADLGCTFWDSSDMYGFGANEECIGRWFKQTGRRKEIFLATKFGYEKNPET 92
Query: 347 SKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIH 451
+L + + PD + AL +L L + +DLY +H
Sbjct: 93 GEL-SLNNEPDYIEKALDLSLKRLGIDCIDLYYVH 126
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 6.6
Identities = 18/44 (40%), Positives = 25/44 (56%)
Frame = +1
Query: 412 QSERSVLGSVPDTLAPGLQGRRLSLPDRPIR*NPVLGTSITWTP 543
QS + V+G V DTLA G+ R+ SL D + N V+ + W P
Sbjct: 521 QSNKPVMGIVQDTLA-GV--RKFSLRDNFLTRNAVMNIML-WVP 560
>SPBC839.03c |||neddylation protein Dcn1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 26.2 bits (55), Expect = 6.6
Identities = 16/49 (32%), Positives = 22/49 (44%)
Frame = +2
Query: 338 FITSKLWNTFHRPDLVRGALQETLDNLNVQYLDLYLIHWPQAYKEDGSL 484
F+ S FHR V GAL L ++ L L + Q ++ D SL
Sbjct: 97 FLKSPRMGEFHRESFVEGAL--NLSTTSLDQLKLAIKEKVQVWRSDASL 143
>SPAC9E9.17c |||dubiuos|Schizosaccharomyces pombe|chr 1|||Manual
Length = 72
Score = 25.8 bits (54), Expect = 8.7
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = -1
Query: 349 RSYEQIFPRYDSFLNFAGYSFSDFLLVSVNIGTVDVSVANVN 224
RS E + D LN +F+DF ++N+G + +S N
Sbjct: 29 RSREFSVIQKDEELNVLEINFNDFYRTTLNVGELSMSTRKTN 70
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,582,995
Number of Sequences: 5004
Number of extensions: 73411
Number of successful extensions: 225
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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