Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_A03
(847 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20450.1 68415.m02387 60S ribosomal protein L14 (RPL14A) 96 3e-20
At4g27090.1 68417.m03894 60S ribosomal protein L14 (RPL14B) ribo... 95 7e-20
At1g64530.1 68414.m07315 RWP-RK domain-containing protein simila... 30 1.7
At5g02840.2 68418.m00227 myb family transcription factor contain... 29 2.9
At5g02840.1 68418.m00226 myb family transcription factor contain... 29 2.9
At1g01520.1 68414.m00068 myb family transcription factor similar... 29 2.9
At5g15890.1 68418.m01859 expressed protein 29 5.1
At5g18470.1 68418.m02175 curculin-like (mannose-binding) lectin ... 28 6.8
At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 28 6.8
At1g09770.1 68414.m01096 myb family transcription factor contain... 28 6.8
At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase fa... 28 9.0
>At2g20450.1 68415.m02387 60S ribosomal protein L14 (RPL14A)
Length = 134
Score = 95.9 bits (228), Expect = 3e-20
Identities = 51/119 (42%), Positives = 73/119 (61%)
Frame = +3
Query: 90 MPFARYVEPGRVALVADGPLKGKLVSVVDVIDQTRALVDGPGSGVPRQQIRLNQLHLTKF 269
M F R+VE GRVALV G GKLV +VDV+DQ RALVD P + R Q+ L +L LT
Sbjct: 1 MGFKRFVEIGRVALVNYGEDYGKLVVIVDVVDQNRALVDAP--DMERIQMNLKRLSLTDI 58
Query: 270 RLKYAFTAPTRLVRKAWTDAKLNEKWTESQWAQKLANKEKRAQMTDYDRFKLTAARVKR 446
+ +++ +A A + KW +S W +KL +++RA + D+DRFK+ A++KR
Sbjct: 59 VIDINRVPKKKVLIEAMEKADVKNKWEKSSWGRKLIVQKRRAALNDFDRFKIMLAKIKR 117
>At4g27090.1 68417.m03894 60S ribosomal protein L14 (RPL14B)
ribosomal protein L14 - Human,PIR3:JC5954
Length = 134
Score = 94.7 bits (225), Expect = 7e-20
Identities = 50/119 (42%), Positives = 71/119 (59%)
Frame = +3
Query: 90 MPFARYVEPGRVALVADGPLKGKLVSVVDVIDQTRALVDGPGSGVPRQQIRLNQLHLTKF 269
M F RYVE GRVALV G GKLV +VDV+DQ RALVD P + R Q+ +L LT
Sbjct: 1 MGFKRYVEIGRVALVNYGEDHGKLVVIVDVVDQNRALVDAP--DMERIQMNFKRLSLTDI 58
Query: 270 RLKYAFTAPTRLVRKAWTDAKLNEKWTESQWAQKLANKEKRAQMTDYDRFKLTAARVKR 446
+ + + +A A + KW +S W +KL +++RA + D+DRFK+ A++K+
Sbjct: 59 VIDINRVPKKKALIEAMEKADVKNKWEKSSWGRKLIVQKRRANLNDFDRFKIMLAKIKK 117
>At1g64530.1 68414.m07315 RWP-RK domain-containing protein similar to
nodule inception protein [Lotus japonicus] GI:6448579;
contains Pfam profile: PF02042 RWP-RK domain
Length = 841
Score = 30.3 bits (65), Expect = 1.7
Identities = 38/149 (25%), Positives = 59/149 (39%), Gaps = 15/149 (10%)
Frame = +3
Query: 93 PFARYVEPGRVALVADGP--LKGKLVSVVDVIDQTRALVDGPGSGVPRQQIRLNQLHLTK 266
P+ + P + L + P L ++ DV + R D GS R ++N + T+
Sbjct: 634 PWPHQIPPIDIQLAKNCPPTSTSPLSNLQDVKIENRDAEDSAGSSTSRASCKVNPICETR 693
Query: 267 FRLKYAFTAPTRLVRKAWTDAKLNEKWTESQWA----QKLANKE---------KRAQMTD 407
FRL P+R V A D+ + K + WA Q A+ K D
Sbjct: 694 FRLPTHNQEPSRQV--ALDDSDSSSKNMTNFWAHLTCQDTASPTILQHKLVSIKATYRED 751
Query: 408 YDRFKLTAARVKRNRARTAVFKSLKVKAA 494
RFK++ V + V K LK++ A
Sbjct: 752 IIRFKISPESVSITELKQQVAKRLKLETA 780
>At5g02840.2 68418.m00227 myb family transcription factor contains
PFAM profile: PF00249 myb-like DNA binding domain
Length = 293
Score = 29.5 bits (63), Expect = 2.9
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +3
Query: 291 APTRLVRKAWTDAKLNEKWTESQ 359
AP + VRKA+T K E WTE +
Sbjct: 33 APEKKVRKAYTITKSRESWTEGE 55
>At5g02840.1 68418.m00226 myb family transcription factor contains
PFAM profile: PF00249 myb-like DNA binding domain
Length = 293
Score = 29.5 bits (63), Expect = 2.9
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +3
Query: 291 APTRLVRKAWTDAKLNEKWTESQ 359
AP + VRKA+T K E WTE +
Sbjct: 33 APEKKVRKAYTITKSRESWTEGE 55
>At1g01520.1 68414.m00068 myb family transcription factor similar to
myb-related protein GI:2505876 from [Arabidopsis
thaliana]
Length = 287
Score = 29.5 bits (63), Expect = 2.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +3
Query: 285 FTAPTRLVRKAWTDAKLNEKWTESQ 359
F PT+ VRK +T K E WTE +
Sbjct: 44 FEDPTKKVRKPYTITKSRENWTEQE 68
>At5g15890.1 68418.m01859 expressed protein
Length = 526
Score = 28.7 bits (61), Expect = 5.1
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = -1
Query: 214 PGPSTSARVWSITSTTLTNFPFKGPSATRATRP 116
P P T +VW+ TS T P +AT+P
Sbjct: 286 PSPDTDFKVWNYTSYNFTLHVMWSPFLVKATKP 318
>At5g18470.1 68418.m02175 curculin-like (mannose-binding) lectin
family protein contains Pfam profile: PF01453 lectin
(probable mannose binding)
Length = 413
Score = 28.3 bits (60), Expect = 6.8
Identities = 18/63 (28%), Positives = 26/63 (41%), Gaps = 2/63 (3%)
Frame = +2
Query: 5 IXYDLTIGNS*DLLFESTFVCVKTL*ERHAFCTVRRTRACRPGSR--RSLKGKVSERGRR 178
+ + L+ NS + ES+ C L A C + ACR GS +G + E
Sbjct: 289 VTFSLSSNNSSTWISESSEPCKTDLKNSSAICITEKPTACRKGSEYFEPRRGYMMENNTG 348
Query: 179 Y*P 187
Y P
Sbjct: 349 YYP 351
>At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar
to 60S ribosomal protein L6 GI:7208784 from [Cicer
arietinum]
Length = 233
Score = 28.3 bits (60), Expect = 6.8
Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
Frame = +3
Query: 108 VEPGRVALVADGPLKGKLVSVVDVIDQTRALVDGPG--SGVPRQQIRLNQLHL 260
+ PG V ++ G KGK V + + LV GP +GVP + R+NQ ++
Sbjct: 89 ITPGTVLIILAGRFKGKRVVFLKQLSSGLLLVTGPFKINGVPLR--RVNQAYV 139
>At1g09770.1 68414.m01096 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 844
Score = 28.3 bits (60), Expect = 6.8
Identities = 19/81 (23%), Positives = 37/81 (45%)
Frame = +3
Query: 282 AFTAPTRLVRKAWTDAKLNEKWTESQWAQKLANKEKRAQMTDYDRFKLTAARVKRNRART 461
AF VRK + + + ++++ E+RA+ T + + + T + +
Sbjct: 692 AFQEEMENVRKKMEEDEKKAEHMKAKYKTYTKGHERRAE-TVWTQIEATLKQAEIGGTEV 750
Query: 462 AVFKSLKVKAARAGTFGKKNI 524
FK+LK + A +F KKN+
Sbjct: 751 ECFKALKRQEEMAASFRKKNL 771
>At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 530
Score = 27.9 bits (59), Expect = 9.0
Identities = 17/60 (28%), Positives = 25/60 (41%)
Frame = +3
Query: 240 RLNQLHLTKFRLKYAFTAPTRLVRKAWTDAKLNEKWTESQWAQKLANKEKRAQMTDYDRF 419
R + +H K RL+ A PT + D L + S + +E A + DY RF
Sbjct: 64 RSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSSSVRVGETGFREVAAYLLDYGRF 123
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,834,859
Number of Sequences: 28952
Number of extensions: 276680
Number of successful extensions: 738
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 691
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 736
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1960634400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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