Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_P15
         (832 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosac...    52   8e-08
SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosacch...    50   4e-07
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote...    28   1.9  
SPBC36.03c |||spermidine family transporter |Schizosaccharomyces...    26   7.5  

>SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein
           L27|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 136

 Score = 52.4 bits (120), Expect = 8e-08
 Identities = 42/130 (32%), Positives = 66/130 (50%), Gaps = 6/130 (4%)
 Frame = +3

Query: 99  G*VVLVLSGRYAGRKAIVVKXL----RRRYFRQAVRACFRRWYRQVPPGKVHKEDGXRIX 266
           G V L+  GR+AG+K ++++ +    +   F  AV A   R+     P KV K  G +  
Sbjct: 8   GKVALITRGRFAGKKVVILQAIDQGSKSHPFGHAVVAGVERY-----PLKVTKSMGAKRI 62

Query: 267 STKRSR*AFRERL*TYNHLMPP--VIQLTSPLKNFSAKXPERPCKR*EAAFXHASRFEER 440
           + +RSR     ++  YNHLMP    ++L +     +A   + P +R  A       FEE+
Sbjct: 63  A-RRSRVKPFIKVVNYNHLMPTRYALELDNLKGLITADTFKEPTQRSAARKTVKKTFEEK 121

Query: 441 YKSGKNKWFF 470
           Y+SGK+ WFF
Sbjct: 122 YQSGKSAWFF 131



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 22/45 (48%), Positives = 30/45 (66%)
 Frame = +1

Query: 166 DEGTSDKPYGHAFVAGIDRYPPGKCTKRMGXE*XPQRGPDKPFVK 300
           D+G+   P+GHA VAG++RYP  K TK MG +   +R   KPF+K
Sbjct: 30  DQGSKSHPFGHAVVAGVERYPL-KVTKSMGAKRIARRSRVKPFIK 73


>SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein
           L27|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 136

 Score = 50.0 bits (114), Expect = 4e-07
 Identities = 44/130 (33%), Positives = 63/130 (48%), Gaps = 6/130 (4%)
 Frame = +3

Query: 99  G*VVLVLSGRYAGRKAIVVKXL----RRRYFRQAVRACFRRWYRQVPPGKVHKEDGXRIX 266
           G V LV  GR+AG+K ++++ +    +   F  AV A   R+     P KV K  G +  
Sbjct: 8   GKVALVTRGRFAGKKVVILQNVDQGSKSHPFGHAVVAGVERY-----PLKVTKSMGAKRI 62

Query: 267 STKRSR*AFRERL*TYNHLMPPVIQLT-SPLKNFSAKXP-ERPCKR*EAAFXHASRFEER 440
           + KRSR     ++  YNHLMP    L    LK          P +R  A     + FEE+
Sbjct: 63  A-KRSRVKPFIKVINYNHLMPTRYALELDNLKGLVTPTTFSEPSQRSAAKKTVKNTFEEK 121

Query: 441 YKSGKNKWFF 470
           Y++GK+ WFF
Sbjct: 122 YQTGKSAWFF 131



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 22/45 (48%), Positives = 30/45 (66%)
 Frame = +1

Query: 166 DEGTSDKPYGHAFVAGIDRYPPGKCTKRMGXE*XPQRGPDKPFVK 300
           D+G+   P+GHA VAG++RYP  K TK MG +   +R   KPF+K
Sbjct: 30  DQGSKSHPFGHAVVAGVERYPL-KVTKSMGAKRIAKRSRVKPFIK 73


>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
           Mug36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1646

 Score = 27.9 bits (59), Expect = 1.9
 Identities = 10/17 (58%), Positives = 13/17 (76%)
 Frame = -3

Query: 248 LFVHFPGGYLSIPATKA 198
           LF+H PGG +S+P  KA
Sbjct: 481 LFIHIPGGEISLPLGKA 497


>SPBC36.03c |||spermidine family transporter |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 538

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 16/55 (29%), Positives = 27/55 (49%)
 Frame = -3

Query: 407 SFLPFAGSFRSFGAEIFQRRSQLYNGWHQVIIGLQPFTKGLSGPLCGXYSXPILF 243
           + L F     S+G+ +F   + ++   +   IGL     G+S  +CG  S PIL+
Sbjct: 101 ALLAFTTLTASWGSSVFSAAATIFAAKYH--IGLTVALLGMSLYVCGFASGPILW 153


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,444,592
Number of Sequences: 5004
Number of extensions: 39672
Number of successful extensions: 69
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 67
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)