Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_P05
(926 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 92 4e-19
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 92 4e-19
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 91 1e-18
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 88 9e-18
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 75 7e-14
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 71 8e-13
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 71 8e-13
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 69 3e-12
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 66 3e-11
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 66 4e-11
At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial, p... 51 9e-07
At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)... 45 6e-05
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 92.3 bits (219), Expect = 4e-19
Identities = 60/155 (38%), Positives = 69/155 (44%), Gaps = 1/155 (0%)
Frame = +2
Query: 317 KEKDKXXXXVXGXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXX 496
KE+ V G DLG CVGV KNG VE I N G V D E LIG+
Sbjct: 28 KEEATKLGSVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFT-DSERLIGEAA 86
Query: 497 KXXXXXXXEXXVFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXGE-KVF 673
K E VFD K IG ++ V D K ++V K K + V GE KVF
Sbjct: 87 KNQAAVNPERTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNKDGKPYIQVKIKDGETKVF 146
Query: 674 GXEEXXXMVXXKMKENXEXXXGKKVXXXVVXVXXY 778
EE M+ KMKE E GKK+ VV V Y
Sbjct: 147 SPEEISAMILTKMKETAEAYLGKKIKDAVVTVPAY 181
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 92.3 bits (219), Expect = 4e-19
Identities = 60/155 (38%), Positives = 69/155 (44%), Gaps = 1/155 (0%)
Frame = +2
Query: 317 KEKDKXXXXVXGXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXX 496
KE+ V G DLG CVGV KNG VE I N G V D E LIG+
Sbjct: 28 KEEATKLGSVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFT-DSERLIGEAA 86
Query: 497 KXXXXXXXEXXVFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXGE-KVF 673
K E VFD K IG ++ V D K ++V K K + V GE KVF
Sbjct: 87 KNQAAVNPERTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNKDGKPYIQVKIKDGETKVF 146
Query: 674 GXEEXXXMVXXKMKENXEXXXGKKVXXXVVXVXXY 778
EE M+ KMKE E GKK+ VV V Y
Sbjct: 147 SPEEISAMILTKMKETAEAYLGKKIKDAVVTVPAY 181
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 90.6 bits (215), Expect = 1e-18
Identities = 58/146 (39%), Positives = 66/146 (45%), Gaps = 1/146 (0%)
Frame = +2
Query: 344 VXGXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXE 523
V G DLG CVGV KNG VE I N G V D E LIG+ K E
Sbjct: 37 VIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFT-DSERLIGEAAKNQAAVNPE 95
Query: 524 XXVFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXGE-KVFGXEEXXXMV 700
VFD K IG ++ V D K ++V K K + V GE KVF EE M+
Sbjct: 96 RTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNKDGKPYIQVKIKDGETKVFSPEEISAMI 155
Query: 701 XXKMKENXEXXXGKKVXXXVVXVXXY 778
KMKE E GKK+ VV V Y
Sbjct: 156 LTKMKETAEAYLGKKIKDAVVTVPAY 181
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 87.8 bits (208), Expect = 9e-18
Identities = 54/145 (37%), Positives = 64/145 (44%)
Frame = +2
Query: 344 VXGXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXE 523
V G DLG CVGV N VE I N G V D E LIG+ K E
Sbjct: 52 VIGIDLGTTYSCVGVYHNKHVEIIANDQGNRITPSWVAFT-DTERLIGEAAKNQAAKNPE 110
Query: 524 XXVFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXGEKVFGXEEXXXMVX 703
+FD K IG ++ V D+K KVV K K + V EK+F EE M+
Sbjct: 111 RTIFDPKRLIGRKFDDPDVQRDIKFLPYKVVNKDGKPYIQVKVKGEEKLFSPEEISAMIL 170
Query: 704 XKMKENXEXXXGKKVXXXVVXVXXY 778
KMKE E GKK+ V+ V Y
Sbjct: 171 TKMKETAEAFLGKKIKDAVITVPAY 195
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 74.9 bits (176), Expect = 7e-14
Identities = 54/144 (37%), Positives = 60/144 (41%), Gaps = 1/144 (0%)
Frame = +2
Query: 350 GXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXEXX 529
G DLG CVGV N VE I N G V D E LIGD K +
Sbjct: 10 GIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFT-DTERLIGDAAKNQVALNPQNT 68
Query: 530 VFDXKXXIGXEWSXXXVXXDVKXXXXKVVE-KXXKXXVXVXXXXGEKVFGXEEXXXMVXX 706
VFD K IG ++S V D+ KVV K + V EK F EE MV
Sbjct: 69 VFDAKRLIGRKFSDPSVQSDILHWPFKVVSGPGEKPMIVVSYKNEEKQFSPEEISSMVLV 128
Query: 707 KMKENXEXXXGKKVXXXVVXVXXY 778
KMKE E G+ V VV V Y
Sbjct: 129 KMKEVAEAFLGRTVKNAVVTVPAY 152
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 71.3 bits (167), Expect = 8e-13
Identities = 58/170 (34%), Positives = 65/170 (38%), Gaps = 1/170 (0%)
Frame = +2
Query: 350 GXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXEXX 529
G DLG CVGV ++ VE I N G V D E LIGD K
Sbjct: 11 GIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMNPTNT 69
Query: 530 VFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXG-EKVFGXEEXXXMVXX 706
VFD K IG +S V D KVV + + V G EK F EE MV
Sbjct: 70 VFDAKRLIGRRYSDPSVQADKSHWPFKVVSGPGEKPMIVVNHKGEEKQFSAEEISSMVLI 129
Query: 707 KMKENXEXXXGKKVXXXVVXVXXYLMMXXVKXXKMXVXFXXXNVMXNIXE 856
KM+E E G V VV V Y + K NVM I E
Sbjct: 130 KMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINE 179
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 71.3 bits (167), Expect = 8e-13
Identities = 51/144 (35%), Positives = 59/144 (40%), Gaps = 1/144 (0%)
Frame = +2
Query: 350 GXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXEXX 529
G DLG CVGV ++ VE I N G V D E LIGD K
Sbjct: 11 GIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMNPVNT 69
Query: 530 VFDXKXXIGXEWSXXXVXXDVKXXXXKVVE-KXXKXXVXVXXXXGEKVFGXEEXXXMVXX 706
VFD K IG +S V D+K KV + K + V EK F EE MV
Sbjct: 70 VFDAKRLIGRRFSDASVQSDMKFWPFKVTPGQADKPMIFVNYKGEEKQFAAEEISSMVLI 129
Query: 707 KMKENXEXXXGKKVXXXVVXVXXY 778
KM+E E G + VV V Y
Sbjct: 130 KMREIAEAYLGSSIKNAVVTVPAY 153
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 69.3 bits (162), Expect = 3e-12
Identities = 56/170 (32%), Positives = 65/170 (38%), Gaps = 1/170 (0%)
Frame = +2
Query: 350 GXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXEXX 529
G DLG CVGV ++ VE I N G V D E LIGD K
Sbjct: 11 GIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMNPVNT 69
Query: 530 VFDXKXXIGXEWSXXXVXXDVKXXXXKV-VEKXXKXXVXVXXXXGEKVFGXEEXXXMVXX 706
VFD K IG +S V D+K K+ K + V EK F EE MV
Sbjct: 70 VFDAKRLIGRRFSDSSVQSDMKLWPFKIQAGPADKPMIYVEYKGEEKEFAAEEISSMVLI 129
Query: 707 KMKENXEXXXGKKVXXXVVXVXXYLMMXXVKXXKMXVXFXXXNVMXNIXE 856
KM+E E G + VV V Y + K NVM I E
Sbjct: 130 KMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINE 179
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 66.1 bits (154), Expect = 3e-11
Identities = 49/144 (34%), Positives = 58/144 (40%), Gaps = 1/144 (0%)
Frame = +2
Query: 350 GXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXEXX 529
G DLG CVGV ++ VE I N G V D E LIGD K
Sbjct: 11 GIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMNPVNT 69
Query: 530 VFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXG-EKVFGXEEXXXMVXX 706
VFD K IG +S V D + ++ + + V G EK F EE MV
Sbjct: 70 VFDAKRLIGRRFSDASVQSDRQLWPFTIISGTAEKPMIVVEYKGEEKQFAAEEISSMVLI 129
Query: 707 KMKENXEXXXGKKVXXXVVXVXXY 778
KM+E E G V VV V Y
Sbjct: 130 KMREIAEAFLGTTVKNAVVTVPAY 153
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 65.7 bits (153), Expect = 4e-11
Identities = 53/170 (31%), Positives = 65/170 (38%), Gaps = 1/170 (0%)
Frame = +2
Query: 350 GXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXEXX 529
G DLG CVGV ++ VE I N G V D E LIGD K
Sbjct: 11 GIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMNPINT 69
Query: 530 VFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXGE-KVFGXEEXXXMVXX 706
VFD K IG ++ V D+K + + + V GE K F EE M+
Sbjct: 70 VFDAKRLIGRRFTDSSVQSDIKLWPFTLKSGPAEKPMIVVNYKGEDKEFSAEEISSMILI 129
Query: 707 KMKENXEXXXGKKVXXXVVXVXXYLMMXXVKXXKMXVXFXXXNVMXNIXE 856
KM+E E G + VV V Y + K NVM I E
Sbjct: 130 KMREIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINE 179
>At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial,
putative / HSP70, mitochondrial, putative strong
similarity to SP|Q01899 Heat shock 70 kDa protein,
mitochondrial precursor {Phaseolus vulgaris}
Length = 682
Score = 51.2 bits (117), Expect = 9e-07
Identities = 41/145 (28%), Positives = 51/145 (35%)
Frame = +2
Query: 344 VXGXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXE 523
V G DLG CV V + I N G V GE L+G K
Sbjct: 54 VIGIDLGTTNSCVSVMEGKTARVIENAEGSRTTPSVVAMNQKGELLVGTPAKRQAVTNPT 113
Query: 524 XXVFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXGEKVFGXEEXXXMVX 703
+F K IG + ++K K+V K V G+K F + V
Sbjct: 114 NTIFGSKRLIGRRFDDPQTQKEMKMVPYKIV-KAPNGDAWV-EANGQK-FSPSQIGANVL 170
Query: 704 XKMKENXEXXXGKKVXXXVVXVXXY 778
KMKE E GK + VV V Y
Sbjct: 171 TKMKETAEAYLGKSINKAVVTVPAY 195
>At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)
identical to heat shock protein 70 [Arabidopsis
thaliana] GI:6746590
Length = 682
Score = 45.2 bits (102), Expect = 6e-05
Identities = 38/145 (26%), Positives = 52/145 (35%)
Frame = +2
Query: 344 VXGXDLGXXXXCVGVXKNGXVEXIXNXXGXXXXXXXVXXXXDGEXLIGDXXKXXXXXXXE 523
V G DLG CV V + + I N G V GE L+G K
Sbjct: 59 VIGIDLGTTNSCVAVMEGKNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTNPT 118
Query: 524 XXVFDXKXXIGXEWSXXXVXXDVKXXXXKVVEKXXKXXVXVXXXXGEKVFGXEEXXXMVX 703
V K IG ++ ++K K+V + V G++ + + +
Sbjct: 119 NTVSGTKRLIGRKFDDPQTQKEMKMVPYKIV-RAPNGDAWV-EANGQQ-YSPSQIGAFIL 175
Query: 704 XKMKENXEXXXGKKVXXXVVXVXXY 778
KMKE E GK V VV V Y
Sbjct: 176 TKMKETAEAYLGKSVTKAVVTVPAY 200
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,086,464
Number of Sequences: 28952
Number of extensions: 46673
Number of successful extensions: 51
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2207676696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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