Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_P01
(863 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q7T2P7 Cluster: Glutamine synthetase; n=4; Coelomata|Re... 83 7e-15
UniRef50_P15104 Cluster: Glutamine synthetase; n=312; cellular o... 79 1e-13
UniRef50_Q43127 Cluster: Glutamine synthetase, chloroplast/mitoc... 67 6e-10
UniRef50_Q42688 Cluster: Glutamine synthetase cytosolic isozyme;... 65 2e-09
UniRef50_A1L199 Cluster: Glutamine synthetase; n=24; Eukaryota|R... 62 1e-08
UniRef50_O96463 Cluster: Glutamine synthetase; n=1; Skeletonema ... 54 6e-06
UniRef50_Q874T6 Cluster: Glutamine synthetase; n=5; Fungi/Metazo... 53 8e-06
UniRef50_Q5UR44 Cluster: Putative glutamine synthetase; n=1; Aca... 51 3e-05
UniRef50_A0DB13 Cluster: Chromosome undetermined scaffold_44, wh... 50 6e-05
UniRef50_Q7M314 Cluster: Glutamate-ammonia ligase; n=1; Bos taur... 49 1e-04
UniRef50_UPI0000F20B38 Cluster: PREDICTED: hypothetical protein;... 44 0.004
UniRef50_A0PCY1 Cluster: Glutamine synthetase precursor; n=1; Gu... 44 0.007
>UniRef50_Q7T2P7 Cluster: Glutamine synthetase; n=4; Coelomata|Rep:
Glutamine synthetase - Danio rerio (Zebrafish)
(Brachydanio rerio)
Length = 371
Score = 83.4 bits (197), Expect = 7e-15
Identities = 48/158 (30%), Positives = 68/158 (43%)
Frame = +1
Query: 220 SPNAFLSKTLLDRYXDLPLPEDXVLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNY 399
S ++ LSK + +Y +LP D V A Y+WIDG+GE +RC TL+ P++ +DLP WN+
Sbjct: 4 SASSQLSKVVKQQYMELP-QGDQVQAMYIWIDGTGEGLRCKTRTLDSEPKSIEDLPEWNF 62
Query: 400 XXXXXXXXXXXXXXXHLKPQAIYRXPFRRGNXVLVXCXXYXYSXXXXKTXXRIXCXXXYX 579
+L P A++R PFR+ LV C Y+ +T R C
Sbjct: 63 DGSSTYQAEGSNSDMYLIPAAMFRDPFRKDPNKLVLCEVVKYNRKTAETNHRHTCKKIME 122
Query: 580 RXXXHXPXFGXXXXXXXLXSXXXXXGXXXXXXPPXXXP 693
P FG L + G P P
Sbjct: 123 MVGHQSPWFGMEQEYTILGTDGHPFGWPSNGFPGPQGP 160
>UniRef50_P15104 Cluster: Glutamine synthetase; n=312; cellular
organisms|Rep: Glutamine synthetase - Homo sapiens
(Human)
Length = 373
Score = 79.0 bits (186), Expect = 1e-13
Identities = 42/130 (32%), Positives = 61/130 (46%)
Frame = +1
Query: 220 SPNAFLSKTLLDRYXDLPLPEDXVLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNY 399
S ++ L+K + Y LP E V A Y+WIDG+GE +RC TL+ P+ ++LP WN+
Sbjct: 4 SASSHLNKGIKQVYMSLPQGEK-VQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNF 62
Query: 400 XXXXXXXXXXXXXXXHLKPQAIYRXPFRRGNXVLVXCXXYXYSXXXXKTXXRIXCXXXYX 579
+L P A++R PFR+ LV C + Y+ +T R C
Sbjct: 63 DGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMD 122
Query: 580 RXXXHXPXFG 609
P FG
Sbjct: 123 MVSNQHPWFG 132
>UniRef50_Q43127 Cluster: Glutamine synthetase,
chloroplast/mitochondrial precursor; n=594;
Viridiplantae|Rep: Glutamine synthetase,
chloroplast/mitochondrial precursor - Arabidopsis
thaliana (Mouse-ear cress)
Length = 430
Score = 66.9 bits (156), Expect = 6e-10
Identities = 31/76 (40%), Positives = 39/76 (51%)
Frame = +1
Query: 283 DXVLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQA 462
D ++A Y+WI GSG +R T+ E P +LP WNY L PQA
Sbjct: 75 DRIIAEYIWIGGSGIDLRSKSRTIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQA 134
Query: 463 IYRXPFRRGNXVLVXC 510
I+R PFR GN +LV C
Sbjct: 135 IFRDPFRGGNNILVIC 150
>UniRef50_Q42688 Cluster: Glutamine synthetase cytosolic isozyme;
n=6; Eukaryota|Rep: Glutamine synthetase cytosolic
isozyme - Chlamydomonas reinhardtii
Length = 382
Score = 64.9 bits (151), Expect = 2e-09
Identities = 30/77 (38%), Positives = 40/77 (51%)
Frame = +1
Query: 289 VLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQAIY 468
+ A YVWI GS VR TL+ P P+DLP WNY +L P++I+
Sbjct: 38 ICAEYVWIGGSMHDVRSKSRTLSTIPTKPEDLPHWNYDGSSTGQAPGHDSEVYLIPRSIF 97
Query: 469 RXPFRRGNXVLVXCXXY 519
+ PFR G+ +LV C Y
Sbjct: 98 KDPFRGGDNILVMCDCY 114
>UniRef50_A1L199 Cluster: Glutamine synthetase; n=24; Eukaryota|Rep:
Glutamine synthetase - Homo sapiens (Human)
Length = 258
Score = 62.5 bits (145), Expect = 1e-08
Identities = 30/102 (29%), Positives = 45/102 (44%)
Frame = +1
Query: 304 VWIDGSGEXVRCXDXTLNYXPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQAIYRXPFR 483
+W G+GE +RC TL+ P+ ++LP WN+ +L P A++R PFR
Sbjct: 1 LWAGGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFR 60
Query: 484 RGNXVLVXCXXYXYSXXXXKTXXRIXCXXXYXRXXXHXPXFG 609
+ LV C + Y+ +T R C P FG
Sbjct: 61 KDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFG 102
>UniRef50_O96463 Cluster: Glutamine synthetase; n=1; Skeletonema
costatum|Rep: Glutamine synthetase - Skeletonema
costatum (Marine centric diatom)
Length = 410
Score = 53.6 bits (123), Expect = 6e-06
Identities = 35/100 (35%), Positives = 47/100 (47%), Gaps = 5/100 (5%)
Frame = +1
Query: 235 LSKTLLDRYXDLPLPEDXVLATYVWIDGSGEXVRCXDXTLNY-XPETPKDLPIWNYXXXX 411
L +++DR+ LP P+D VLA YVW+D GE R TL E LP WN+
Sbjct: 45 LDTSVVDRFSALPYPDDKVLAEYVWVDAKGE-CRSKTRTLPVARTEAVDKLPNWNFDGSS 103
Query: 412 XXXXXXXXXXXHLKPQAIYRXPFR-RGNXV---LVXCXXY 519
L+P I++ PFR R + + LV C Y
Sbjct: 104 TDQAPGDDSEVILRPCRIFKDPFRPRAHGLDNNLVMCDTY 143
>UniRef50_Q874T6 Cluster: Glutamine synthetase; n=5; Fungi/Metazoa
group|Rep: Glutamine synthetase - Kluyveromyces lactis
(Yeast) (Candida sphaerica)
Length = 372
Score = 53.2 bits (122), Expect = 8e-06
Identities = 29/86 (33%), Positives = 41/86 (47%)
Frame = +1
Query: 247 LLDRYXDLPLPEDXVLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNYXXXXXXXXX 426
+L +Y +L ++A YVWID G +R TLN + LP WN+
Sbjct: 13 ILQKYLELD-QRGAIIAEYVWIDSEG-GLRSKGRTLNKKVTSVDSLPEWNFDGSSTGQAP 70
Query: 427 XXXXXXHLKPQAIYRXPFRRGNXVLV 504
+LKP A Y PFRRG+ ++V
Sbjct: 71 GHDSDIYLKPVAFYPDPFRRGDNIVV 96
>UniRef50_Q5UR44 Cluster: Putative glutamine synthetase; n=1;
Acanthamoeba polyphaga mimivirus|Rep: Putative glutamine
synthetase - Mimivirus
Length = 353
Score = 51.2 bits (117), Expect = 3e-05
Identities = 33/107 (30%), Positives = 44/107 (41%), Gaps = 4/107 (3%)
Frame = +1
Query: 301 YVWIDGSGEXVRCXDXTLNYXPETP---KDLPIWNYXXXXXXXXXXXXXXXHLKPQAIYR 471
YVWI G+GE +R L T D+P+WNY L P+ IYR
Sbjct: 23 YVWIGGNGE-LRSKTRVLYSSIMTGYKLSDIPVWNYDGSSTNQANGSSSEVFLYPRNIYR 81
Query: 472 XPFRRG-NXVLVXCXXYXYSXXXXKTXXRIXCXXXYXRXXXHXPXFG 609
PFRR N V+V C Y + +T R + + P +G
Sbjct: 82 CPFRRNVNGVIVICDTYDVNGVPLETNHRHNANIIFEKYQNEKPWYG 128
>UniRef50_A0DB13 Cluster: Chromosome undetermined scaffold_44, whole
genome shotgun sequence; n=11; Oligohymenophorea|Rep:
Chromosome undetermined scaffold_44, whole genome
shotgun sequence - Paramecium tetraurelia
Length = 398
Score = 50.4 bits (115), Expect = 6e-05
Identities = 25/77 (32%), Positives = 34/77 (44%)
Frame = +1
Query: 289 VLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQAIY 468
VLA Y+WIDG+GE +R + +DL W Y +LKP +
Sbjct: 25 VLAEYIWIDGTGEQLRSKTKVYQTQIKRLEDLEWWTYDGSSTDQAVTRFSEIYLKPVRVV 84
Query: 469 RXPFRRGNXVLVXCXXY 519
+ PFR +LV C Y
Sbjct: 85 KDPFRGDPHILVLCETY 101
>UniRef50_Q7M314 Cluster: Glutamate-ammonia ligase; n=1; Bos
taurus|Rep: Glutamate-ammonia ligase - Bos taurus
(Bovine)
Length = 149
Score = 49.2 bits (112), Expect = 1e-04
Identities = 36/110 (32%), Positives = 48/110 (43%)
Frame = +1
Query: 235 LSKTLLDRYXDLPLPEDXVLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNYXXXXX 414
L K + + Y LP D V A Y+WIDG+GE +RC TL P+ P + Y
Sbjct: 1 LBKGIKZVYMALP-QGDKVQAMYIWIDGTGEGLRCKTRTLXSXPKKPASTNLZRY----- 54
Query: 415 XXXXXXXXXXHLKPQAIYRXPFRRGNXVLVXCXXYXYSXXXXKTXXRIXC 564
L P A++R PF LV C + Y+ +T C
Sbjct: 55 -----------LVPAAMFRDPFXXDPNXLVFCEVFXYNKRPAETNLXXTC 93
>UniRef50_UPI0000F20B38 Cluster: PREDICTED: hypothetical protein;
n=1; Danio rerio|Rep: PREDICTED: hypothetical protein -
Danio rerio
Length = 277
Score = 44.4 bits (100), Expect = 0.004
Identities = 33/130 (25%), Positives = 52/130 (40%)
Frame = +1
Query: 220 SPNAFLSKTLLDRYXDLPLPEDXVLATYVWIDGSGEXVRCXDXTLNYXPETPKDLPIWNY 399
S ++ L+K L RY +LP D L TYVWID G + T++ P+ D+P W+
Sbjct: 5 SESSHLNKFLRHRYLNLP-QGDFCLVTYVWIDSCGVDLYSKTRTMDCEPKILADVPEWD- 62
Query: 400 XXXXXXXXXXXXXXXHLKPQAIYRXPFRRGNXVLVXCXXYXYSXXXXKTXXRIXCXXXYX 579
H++ ++R PF L+ C ++ + R C
Sbjct: 63 VGLETEESSSEMLLNHVR---MFRDPFFLDPNKLILCEVLKHTREPAEWNHRNRCNTLME 119
Query: 580 RXXXHXPXFG 609
+ P FG
Sbjct: 120 KVKDLHPWFG 129
>UniRef50_A0PCY1 Cluster: Glutamine synthetase precursor; n=1;
Guillardia theta|Rep: Glutamine synthetase precursor -
Guillardia theta (Cryptomonas phi)
Length = 160
Score = 43.6 bits (98), Expect = 0.007
Identities = 22/75 (29%), Positives = 33/75 (44%), Gaps = 3/75 (4%)
Frame = +1
Query: 295 ATYVWIDGSG---EXVRCXDXTLNYXPETPKDLPIWNYXXXXXXXXXXXXXXXHLKPQAI 465
A Y+WI G G + R L+ P + +LP+WNY +L+P +
Sbjct: 71 AEYIWIGGRGGCGDDYRSKTRVLDKRPTSVSELPLWNYDGSSTGQAPGGDSEIYLQPAFM 130
Query: 466 YRXPFRRGNXVLVXC 510
P R G+ +LV C
Sbjct: 131 CADPMRGGDNILVLC 145
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 360,955,690
Number of Sequences: 1657284
Number of extensions: 4947756
Number of successful extensions: 10263
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 9964
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10255
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 76652910257
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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