BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_N19
(1066 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 46 7e-06
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.052
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.64
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.84
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 24 1.3
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 29 1.5
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 2.6
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.9
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.8
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 7.8
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 7.8
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 46.4 bits (105), Expect = 7e-06
Identities = 31/110 (28%), Positives = 32/110 (29%), Gaps = 5/110 (4%)
Frame = +1
Query: 709 KPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXX 888
KP PP PPPP R P G PPPPPP
Sbjct: 289 KPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAA 348
Query: 889 XG-----XGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPP 1023
G PPPPPP PP + A P PPP
Sbjct: 349 GSIPLPPQGRSAPPPPPP-----RSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Score = 31.1 bits (67), Expect = 0.28
Identities = 32/131 (24%), Positives = 34/131 (25%), Gaps = 12/131 (9%)
Frame = +1
Query: 694 PPPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXN----PXXXGGGXGXXXPPP 861
PPPP PP + PPPP R + P PP
Sbjct: 337 PPPP------PPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPA 390
Query: 862 PPPXXXXXXX--------GXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXP 1017
PPP PP P P APP A P P
Sbjct: 391 PPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLP 450
Query: 1018 PPXPXXPXPXP 1050
P P P P P
Sbjct: 451 PSAPIAP-PLP 460
Score = 27.5 bits (58), Expect = 3.4
Identities = 16/52 (30%), Positives = 16/52 (30%)
Frame = +3
Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXPXPXPPXPPPPXPXS 1064
PP PPP PP R P P PP PP P S
Sbjct: 388 PPAPPPAIPGRSAPALPPLG-----NASRTSTPPVPTPPSLPPSAPPSLPPS 434
Score = 27.1 bits (57), Expect = 4.5
Identities = 11/29 (37%), Positives = 11/29 (37%)
Frame = +2
Query: 971 PPRPXXPXXXXXXPXXPPXPPXPXPXPXP 1057
P P P P PP P P P P P
Sbjct: 454 PIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 26.6 bits (56), Expect = 5.9
Identities = 22/88 (25%), Positives = 22/88 (25%), Gaps = 6/88 (6%)
Frame = +1
Query: 784 PPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXX 963
PP P P PPP PP P P P
Sbjct: 230 PPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSK 289
Query: 964 XXAPPP------XAXXAXXXXPXPPPXP 1029
PPP A A P PPP P
Sbjct: 290 PPLPPPSSRVSAAALAANKKRPPPPPPP 317
Score = 26.2 bits (55), Expect = 7.8
Identities = 12/28 (42%), Positives = 12/28 (42%), Gaps = 4/28 (14%)
Frame = +1
Query: 346 PPPPPXXFXXS----PPPXXXCXPPPPP 417
PPPPP PP PPPPP
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPP 366
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.5 bits (73), Expect = 0.052
Identities = 15/29 (51%), Positives = 15/29 (51%)
Frame = +1
Query: 841 GXXXPPPPPPXXXXXXXGXGLXXPPPPPP 927
G PPPPPP G G PPPPPP
Sbjct: 759 GGPPPPPPPPGVA----GAGPPPPPPPPP 783
Score = 33.1 bits (72), Expect = 0.068
Identities = 19/59 (32%), Positives = 19/59 (32%)
Frame = +1
Query: 853 PPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPPXP 1029
PPPPPP PPP P PPP A P PPP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAP--------IMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 32.3 bits (70), Expect = 0.12
Identities = 17/52 (32%), Positives = 17/52 (32%), Gaps = 3/52 (5%)
Frame = +3
Query: 912 PPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXP---XPXPPXPPPPXP 1058
PPPPP P PP P P PP PPPP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 31.5 bits (68), Expect = 0.21
Identities = 15/50 (30%), Positives = 15/50 (30%)
Frame = +1
Query: 901 LXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPPXPXXPXPXP 1050
L PPPPP PPP P PPP P P
Sbjct: 728 LLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 30.7 bits (66), Expect = 0.36
Identities = 15/45 (33%), Positives = 15/45 (33%)
Frame = +1
Query: 784 PPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXXXGXGLXXPPP 918
PP P P G G PPPPPP G P P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 30.3 bits (65), Expect = 0.48
Identities = 18/55 (32%), Positives = 18/55 (32%), Gaps = 3/55 (5%)
Frame = +3
Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXPXPX---PPXPPPPXPXS 1064
PPPPPP P PPA P PP PPPP S
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVS 786
Score = 30.3 bits (65), Expect = 0.48
Identities = 11/24 (45%), Positives = 12/24 (50%)
Frame = +1
Query: 346 PPPPPXXFXXSPPPXXXCXPPPPP 417
PPPPP +P P PPP P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAP 756
Score = 29.5 bits (63), Expect = 0.84
Identities = 13/28 (46%), Positives = 13/28 (46%)
Frame = +1
Query: 334 PXXXPPPPPXXFXXSPPPXXXCXPPPPP 417
P P PPP PPP PPPPP
Sbjct: 746 PAPIPVPPPAPIMGGPPP-----PPPPP 768
Score = 29.1 bits (62), Expect = 1.1
Identities = 14/46 (30%), Positives = 14/46 (30%)
Frame = +1
Query: 784 PPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXXXGXGLXXPPPP 921
PPP P G PPPPPP G P P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 28.7 bits (61), Expect = 1.5
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = +1
Query: 346 PPPPPXXFXXSPPPXXXCXPPPPP 417
PPPPP + PP PPPPP
Sbjct: 763 PPPPPPGVAGAGPPP---PPPPPP 783
Score = 27.5 bits (58), Expect = 3.4
Identities = 13/27 (48%), Positives = 14/27 (51%), Gaps = 3/27 (11%)
Frame = +3
Query: 345 PPAPPLXXXXLPPPXXXV---XPPPPP 416
PPAP + PPP V PPPPP
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 26.2 bits (55), Expect = 7.8
Identities = 10/24 (41%), Positives = 10/24 (41%)
Frame = +3
Query: 345 PPAPPLXXXXLPPPXXXVXPPPPP 416
PP PP P P PPP P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAP 756
Score = 26.2 bits (55), Expect = 7.8
Identities = 10/24 (41%), Positives = 10/24 (41%)
Frame = +1
Query: 346 PPPPPXXFXXSPPPXXXCXPPPPP 417
P P P PP PPPPP
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPP 765
Score = 26.2 bits (55), Expect = 7.8
Identities = 12/42 (28%), Positives = 12/42 (28%)
Frame = +3
Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXPXPXP 1034
PPPPPP PP P P P P
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.9 bits (64), Expect = 0.64
Identities = 28/118 (23%), Positives = 28/118 (23%)
Frame = +1
Query: 697 PPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPXX 876
PP KP P PP P P G PP P P
Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGA----PPVPKPSV 1147
Query: 877 XXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPPXPXXPXPXP 1050
PP P P PP A P PPP P P P
Sbjct: 1148 AAPPVPAPSGAPPVPKPSVAAPPVPAPSSGI--PPVPKPAAGVPPVPPPSEAPPVPKP 1203
Score = 29.1 bits (62), Expect = 1.1
Identities = 20/77 (25%), Positives = 21/77 (27%)
Frame = +1
Query: 697 PPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPXX 876
PP KP P V PP P P PP PPP
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAG---VPPVPPPSE 1196
Query: 877 XXXXXGXGLXXPPPPPP 927
+ PP PPP
Sbjct: 1197 APPVPKPSVGVPPVPPP 1213
Score = 28.7 bits (61), Expect = 1.5
Identities = 26/118 (22%), Positives = 28/118 (23%), Gaps = 1/118 (0%)
Frame = +1
Query: 694 PPPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPX 873
PP P PP + + PP P G PPP P
Sbjct: 1012 PPVPKLSSKAPPVPLPSADAPPI---PVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPA 1068
Query: 874 XXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPP-PXAXXAXXXXPXPPPXPXXPXP 1044
PP P APP P A P P P P P
Sbjct: 1069 PSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKP 1126
Score = 27.1 bits (57), Expect = 4.5
Identities = 18/73 (24%), Positives = 20/73 (27%), Gaps = 7/73 (9%)
Frame = +1
Query: 853 PPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAP-------PPXAXXAXXXXP 1011
PP P P + PP P P P PP + P
Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPP 1209
Query: 1012 XPPPXPXXPXPXP 1050
PPP P P P
Sbjct: 1210 VPPPSTAPPVPTP 1222
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.5 bits (63), Expect = 0.84
Identities = 13/28 (46%), Positives = 13/28 (46%)
Frame = +1
Query: 334 PXXXPPPPPXXFXXSPPPXXXCXPPPPP 417
P PPPPP PP PPPPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQP--PPPPPP 30
Score = 28.3 bits (60), Expect = 1.9
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +1
Query: 841 GXXXPPPPPPXXXXXXXGXGLXXPPPPPP 927
G PPPPPP PPPPPP
Sbjct: 7 GNPPPPPPPPGFEPPS-----QPPPPPPP 30
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 24.2 bits (50), Expect(2) = 1.3
Identities = 8/10 (80%), Positives = 8/10 (80%)
Frame = +1
Query: 898 GLXXPPPPPP 927
GL PPPPPP
Sbjct: 304 GLPPPPPPPP 313
Score = 23.0 bits (47), Expect(2) = 1.3
Identities = 8/18 (44%), Positives = 8/18 (44%)
Frame = +1
Query: 817 PXXXGGGXGXXXPPPPPP 870
P G PPPPPP
Sbjct: 295 PSSGDSANGGLPPPPPPP 312
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 28.7 bits (61), Expect = 1.5
Identities = 17/51 (33%), Positives = 17/51 (33%)
Frame = -1
Query: 1051 GGGGXGGXGXGXGXXXXXXRXXRXAGGXXXGGXXXXXXXXXXGGGGGGXXA 899
GGGG GG G G G GG GGGG G A
Sbjct: 135 GGGGMGGGMGGMGGMDDDMDMDGGFGTRTRGGGMPGGFANMFGGGGAGPHA 185
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.9 bits (59), Expect = 2.6
Identities = 16/53 (30%), Positives = 17/53 (32%), Gaps = 5/53 (9%)
Frame = +3
Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXX-----PXPXPXPPXPPPP 1052
PPPPPP P + R P P PP PPPP
Sbjct: 192 PPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQADPLPAPPPPPPP 244
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 5.9
Identities = 19/71 (26%), Positives = 20/71 (28%)
Frame = +1
Query: 832 GGXGXXXPPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXP 1011
GG P PP + P PPPP A PP A
Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSA-------- 1730
Query: 1012 XPPPXPXXPXP 1044
PPP P P
Sbjct: 1731 PPPPLPASSAP 1741
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 331
Score = 26.2 bits (55), Expect = 7.8
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 1/30 (3%)
Frame = +2
Query: 971 PPRPXXPXXXXXXPXXPPXPPXPXP-XPXP 1057
PP P P P PP P P P P P
Sbjct: 107 PPLPNEPVPEEPLPGEPPLPDEPVPEEPLP 136
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 273
Score = 26.2 bits (55), Expect = 7.8
Identities = 19/65 (29%), Positives = 19/65 (29%), Gaps = 2/65 (3%)
Frame = -3
Query: 1043 GXGXXGXGGGXGXXXXAXXAXGGGAXXXXXXXXXXXXXXGGGGG--GXXSPXPXXLXXWX 870
G G G GG G GGG GG GG G P
Sbjct: 206 GFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGP 265
Query: 869 GGGGG 855
GG GG
Sbjct: 266 GGHGG 270
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.2 bits (55), Expect = 7.8
Identities = 9/22 (40%), Positives = 10/22 (45%)
Frame = +1
Query: 856 PPPPPXXXXXXXGXGLXXPPPP 921
PPPPP + PPPP
Sbjct: 908 PPPPPTASMTASAPAIASPPPP 929
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,878,554
Number of Sequences: 5004
Number of extensions: 31324
Number of successful extensions: 440
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 246
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 561209266
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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