BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_N19 (1066 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 46 7e-06 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.052 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.64 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.84 SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 24 1.3 SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 29 1.5 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 2.6 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.9 SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.8 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 7.8 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 7.8 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 46.4 bits (105), Expect = 7e-06 Identities = 31/110 (28%), Positives = 32/110 (29%), Gaps = 5/110 (4%) Frame = +1 Query: 709 KPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXX 888 KP PP PPPP R P G PPPPPP Sbjct: 289 KPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAA 348 Query: 889 XG-----XGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPP 1023 G PPPPPP PP + A P PPP Sbjct: 349 GSIPLPPQGRSAPPPPPP-----RSAPSTGRQPPPLSSSRAVSNPPAPPP 393 Score = 31.1 bits (67), Expect = 0.28 Identities = 32/131 (24%), Positives = 34/131 (25%), Gaps = 12/131 (9%) Frame = +1 Query: 694 PPPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXN----PXXXGGGXGXXXPPP 861 PPPP PP + PPPP R + P PP Sbjct: 337 PPPP------PPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPA 390 Query: 862 PPPXXXXXXX--------GXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXP 1017 PPP PP P P APP A P P Sbjct: 391 PPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLP 450 Query: 1018 PPXPXXPXPXP 1050 P P P P P Sbjct: 451 PSAPIAP-PLP 460 Score = 27.5 bits (58), Expect = 3.4 Identities = 16/52 (30%), Positives = 16/52 (30%) Frame = +3 Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXPXPXPPXPPPPXPXS 1064 PP PPP PP R P P PP PP P S Sbjct: 388 PPAPPPAIPGRSAPALPPLG-----NASRTSTPPVPTPPSLPPSAPPSLPPS 434 Score = 27.1 bits (57), Expect = 4.5 Identities = 11/29 (37%), Positives = 11/29 (37%) Frame = +2 Query: 971 PPRPXXPXXXXXXPXXPPXPPXPXPXPXP 1057 P P P P PP P P P P P Sbjct: 454 PIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482 Score = 26.6 bits (56), Expect = 5.9 Identities = 22/88 (25%), Positives = 22/88 (25%), Gaps = 6/88 (6%) Frame = +1 Query: 784 PPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXX 963 PP P P PPP PP P P P Sbjct: 230 PPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSK 289 Query: 964 XXAPPP------XAXXAXXXXPXPPPXP 1029 PPP A A P PPP P Sbjct: 290 PPLPPPSSRVSAAALAANKKRPPPPPPP 317 Score = 26.2 bits (55), Expect = 7.8 Identities = 12/28 (42%), Positives = 12/28 (42%), Gaps = 4/28 (14%) Frame = +1 Query: 346 PPPPPXXFXXS----PPPXXXCXPPPPP 417 PPPPP PP PPPPP Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPP 366 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 33.5 bits (73), Expect = 0.052 Identities = 15/29 (51%), Positives = 15/29 (51%) Frame = +1 Query: 841 GXXXPPPPPPXXXXXXXGXGLXXPPPPPP 927 G PPPPPP G G PPPPPP Sbjct: 759 GGPPPPPPPPGVA----GAGPPPPPPPPP 783 Score = 33.1 bits (72), Expect = 0.068 Identities = 19/59 (32%), Positives = 19/59 (32%) Frame = +1 Query: 853 PPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPPXP 1029 PPPPPP PPP P PPP A P PPP P Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAP--------IMGGPPPPPPPPGVAGAGPPPPPPPP 782 Score = 32.3 bits (70), Expect = 0.12 Identities = 17/52 (32%), Positives = 17/52 (32%), Gaps = 3/52 (5%) Frame = +3 Query: 912 PPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXP---XPXPPXPPPPXP 1058 PPPPP P PP P P PP PPPP P Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 Score = 31.5 bits (68), Expect = 0.21 Identities = 15/50 (30%), Positives = 15/50 (30%) Frame = +1 Query: 901 LXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPPXPXXPXPXP 1050 L PPPPP PPP P PPP P P Sbjct: 728 LLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777 Score = 30.7 bits (66), Expect = 0.36 Identities = 15/45 (33%), Positives = 15/45 (33%) Frame = +1 Query: 784 PPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXXXGXGLXXPPP 918 PP P P G G PPPPPP G P P Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797 Score = 30.3 bits (65), Expect = 0.48 Identities = 18/55 (32%), Positives = 18/55 (32%), Gaps = 3/55 (5%) Frame = +3 Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXPXPX---PPXPPPPXPXS 1064 PPPPPP P PPA P PP PPPP S Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVS 786 Score = 30.3 bits (65), Expect = 0.48 Identities = 11/24 (45%), Positives = 12/24 (50%) Frame = +1 Query: 346 PPPPPXXFXXSPPPXXXCXPPPPP 417 PPPPP +P P PPP P Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAP 756 Score = 29.5 bits (63), Expect = 0.84 Identities = 13/28 (46%), Positives = 13/28 (46%) Frame = +1 Query: 334 PXXXPPPPPXXFXXSPPPXXXCXPPPPP 417 P P PPP PPP PPPPP Sbjct: 746 PAPIPVPPPAPIMGGPPP-----PPPPP 768 Score = 29.1 bits (62), Expect = 1.1 Identities = 14/46 (30%), Positives = 14/46 (30%) Frame = +1 Query: 784 PPPPXXRXXXNPXXXGGGXGXXXPPPPPPXXXXXXXGXGLXXPPPP 921 PPP P G PPPPPP G P P Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797 Score = 28.7 bits (61), Expect = 1.5 Identities = 12/24 (50%), Positives = 13/24 (54%) Frame = +1 Query: 346 PPPPPXXFXXSPPPXXXCXPPPPP 417 PPPPP + PP PPPPP Sbjct: 763 PPPPPPGVAGAGPPP---PPPPPP 783 Score = 27.5 bits (58), Expect = 3.4 Identities = 13/27 (48%), Positives = 14/27 (51%), Gaps = 3/27 (11%) Frame = +3 Query: 345 PPAPPLXXXXLPPPXXXV---XPPPPP 416 PPAP + PPP V PPPPP Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPP 779 Score = 26.2 bits (55), Expect = 7.8 Identities = 10/24 (41%), Positives = 10/24 (41%) Frame = +3 Query: 345 PPAPPLXXXXLPPPXXXVXPPPPP 416 PP PP P P PPP P Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAP 756 Score = 26.2 bits (55), Expect = 7.8 Identities = 10/24 (41%), Positives = 10/24 (41%) Frame = +1 Query: 346 PPPPPXXFXXSPPPXXXCXPPPPP 417 P P P PP PPPPP Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPP 765 Score = 26.2 bits (55), Expect = 7.8 Identities = 12/42 (28%), Positives = 12/42 (28%) Frame = +3 Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXXPXPXPXP 1034 PPPPPP PP P P P P Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.9 bits (64), Expect = 0.64 Identities = 28/118 (23%), Positives = 28/118 (23%) Frame = +1 Query: 697 PPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPXX 876 PP KP P PP P P G PP P P Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGA----PPVPKPSV 1147 Query: 877 XXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXPXPPPXPXXPXPXP 1050 PP P P PP A P PPP P P P Sbjct: 1148 AAPPVPAPSGAPPVPKPSVAAPPVPAPSSGI--PPVPKPAAGVPPVPPPSEAPPVPKP 1203 Score = 29.1 bits (62), Expect = 1.1 Identities = 20/77 (25%), Positives = 21/77 (27%) Frame = +1 Query: 697 PPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPXX 876 PP KP P V PP P P PP PPP Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAG---VPPVPPPSE 1196 Query: 877 XXXXXGXGLXXPPPPPP 927 + PP PPP Sbjct: 1197 APPVPKPSVGVPPVPPP 1213 Score = 28.7 bits (61), Expect = 1.5 Identities = 26/118 (22%), Positives = 28/118 (23%), Gaps = 1/118 (0%) Frame = +1 Query: 694 PPPPXKPXXXPPXXVXXXXXXXVGXXXXXXPPPPXXRXXXNPXXXGGGXGXXXPPPPPPX 873 PP P PP + + PP P G PPP P Sbjct: 1012 PPVPKLSSKAPPVPLPSADAPPI---PVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPA 1068 Query: 874 XXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPP-PXAXXAXXXXPXPPPXPXXPXP 1044 PP P APP P A P P P P P Sbjct: 1069 PSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKP 1126 Score = 27.1 bits (57), Expect = 4.5 Identities = 18/73 (24%), Positives = 20/73 (27%), Gaps = 7/73 (9%) Frame = +1 Query: 853 PPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAP-------PPXAXXAXXXXP 1011 PP P P + PP P P P PP + P Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPP 1209 Query: 1012 XPPPXPXXPXPXP 1050 PPP P P P Sbjct: 1210 VPPPSTAPPVPTP 1222 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 29.5 bits (63), Expect = 0.84 Identities = 13/28 (46%), Positives = 13/28 (46%) Frame = +1 Query: 334 PXXXPPPPPXXFXXSPPPXXXCXPPPPP 417 P PPPPP PP PPPPP Sbjct: 5 PPGNPPPPPPPPGFEPPSQP--PPPPPP 30 Score = 28.3 bits (60), Expect = 1.9 Identities = 13/29 (44%), Positives = 13/29 (44%) Frame = +1 Query: 841 GXXXPPPPPPXXXXXXXGXGLXXPPPPPP 927 G PPPPPP PPPPPP Sbjct: 7 GNPPPPPPPPGFEPPS-----QPPPPPPP 30 >SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|Schizosaccharomyces pombe|chr 3|||Manual Length = 551 Score = 24.2 bits (50), Expect(2) = 1.3 Identities = 8/10 (80%), Positives = 8/10 (80%) Frame = +1 Query: 898 GLXXPPPPPP 927 GL PPPPPP Sbjct: 304 GLPPPPPPPP 313 Score = 23.0 bits (47), Expect(2) = 1.3 Identities = 8/18 (44%), Positives = 8/18 (44%) Frame = +1 Query: 817 PXXXGGGXGXXXPPPPPP 870 P G PPPPPP Sbjct: 295 PSSGDSANGGLPPPPPPP 312 >SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces pombe|chr 3|||Manual Length = 379 Score = 28.7 bits (61), Expect = 1.5 Identities = 17/51 (33%), Positives = 17/51 (33%) Frame = -1 Query: 1051 GGGGXGGXGXGXGXXXXXXRXXRXAGGXXXGGXXXXXXXXXXGGGGGGXXA 899 GGGG GG G G G GG GGGG G A Sbjct: 135 GGGGMGGGMGGMGGMDDDMDMDGGFGTRTRGGGMPGGFANMFGGGGAGPHA 185 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 27.9 bits (59), Expect = 2.6 Identities = 16/53 (30%), Positives = 17/53 (32%), Gaps = 5/53 (9%) Frame = +3 Query: 909 PPPPPPXXXXXXXXXXPPXXXPPAXRXXRXXXXXX-----PXPXPXPPXPPPP 1052 PPPPPP P + R P P PP PPPP Sbjct: 192 PPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQADPLPAPPPPPPP 244 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 26.6 bits (56), Expect = 5.9 Identities = 19/71 (26%), Positives = 20/71 (28%) Frame = +1 Query: 832 GGXGXXXPPPPPPXXXXXXXGXGLXXPPPPPPXXXXXXXXXXXXXXAPPPXAXXAXXXXP 1011 GG P PP + P PPPP A PP A Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSA-------- 1730 Query: 1012 XPPPXPXXPXP 1044 PPP P P Sbjct: 1731 PPPPLPASSAP 1741 >SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 26.2 bits (55), Expect = 7.8 Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 1/30 (3%) Frame = +2 Query: 971 PPRPXXPXXXXXXPXXPPXPPXPXP-XPXP 1057 PP P P P PP P P P P P Sbjct: 107 PPLPNEPVPEEPLPGEPPLPDEPVPEEPLP 136 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 26.2 bits (55), Expect = 7.8 Identities = 19/65 (29%), Positives = 19/65 (29%), Gaps = 2/65 (3%) Frame = -3 Query: 1043 GXGXXGXGGGXGXXXXAXXAXGGGAXXXXXXXXXXXXXXGGGGG--GXXSPXPXXLXXWX 870 G G G GG G GGG GG GG G P Sbjct: 206 GFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGP 265 Query: 869 GGGGG 855 GG GG Sbjct: 266 GGHGG 270 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.2 bits (55), Expect = 7.8 Identities = 9/22 (40%), Positives = 10/22 (45%) Frame = +1 Query: 856 PPPPPXXXXXXXGXGLXXPPPP 921 PPPPP + PPPP Sbjct: 908 PPPPPTASMTASAPAIASPPPP 929 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,878,554 Number of Sequences: 5004 Number of extensions: 31324 Number of successful extensions: 440 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 38 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 246 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 561209266 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -