BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_N14 (895 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.055 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.13 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.29 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.6 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 6.3 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 23 7.4 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 8.3 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 33.1 bits (72), Expect = 0.055 Identities = 18/55 (32%), Positives = 19/55 (34%), Gaps = 4/55 (7%) Frame = +3 Query: 606 RXPPPPPPXXXXKXXPP----XXXXGLXXXPXXXXXXKXPXXXLPPXXNXXPPPP 758 R PPPPPP + PP L P LPP PPPP Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPP 364 Score = 28.3 bits (60), Expect = 1.6 Identities = 29/133 (21%), Positives = 32/133 (24%), Gaps = 2/133 (1%) Frame = +2 Query: 434 PPVXSGAGXGSXLXPRG*XXLPPXXXXXXXEXTXXPKXXXKGPXPXXXXXXXSXFXXKGP 613 PP+ +G+ S P PP P P P S P Sbjct: 325 PPIGNGSSNSSLPPP------PPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378 Query: 614 XPPSSXXXXKXXXXPXXXG--AXXXPPXGXXXXXXXXXXPPPXKXPPPPPXXXXXXXPPP 787 S P G A PP G P P PP P PP Sbjct: 379 LSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPS 438 Query: 788 PXPGXGXXXXXPP 826 G PP Sbjct: 439 LPMGAPAAPPLPP 451 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 31.9 bits (69), Expect = 0.13 Identities = 16/48 (33%), Positives = 16/48 (33%) Frame = +2 Query: 683 PPXGXXXXXXXXXXPPPXKXPPPPPXXXXXXXPPPPXPGXGXXXXXPP 826 PP P P PPP P PPPP PG PP Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAP-IMGGPPPPPPPPGVAGAGPPPP 778 Score = 31.5 bits (68), Expect = 0.17 Identities = 13/24 (54%), Positives = 13/24 (54%) Frame = +2 Query: 725 PPPXKXPPPPPXXXXXXXPPPPXP 796 PPP PPPPP PPPP P Sbjct: 761 PPP---PPPPPGVAGAGPPPPPPP 781 Score = 26.2 bits (55), Expect = 6.3 Identities = 10/23 (43%), Positives = 10/23 (43%) Frame = +2 Query: 728 PPXKXPPPPPXXXXXXXPPPPXP 796 PP PPP PPPP P Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP 783 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 30.7 bits (66), Expect = 0.29 Identities = 12/27 (44%), Positives = 12/27 (44%) Frame = +2 Query: 725 PPPXKXPPPPPXXXXXXXPPPPXPGXG 805 PP PPPPP PPP P G Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPG 31 Score = 23.8 bits (49), Expect(2) = 1.2 Identities = 8/15 (53%), Positives = 8/15 (53%) Frame = +3 Query: 612 PPPPPPXXXXKXXPP 656 PPPPPP PP Sbjct: 11 PPPPPPGFEPPSQPP 25 Score = 23.0 bits (47), Expect(2) = 1.2 Identities = 7/10 (70%), Positives = 7/10 (70%) Frame = +3 Query: 600 PXRXPPPPPP 629 P PPPPPP Sbjct: 6 PGNPPPPPPP 15 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.3 bits (60), Expect = 1.6 Identities = 12/26 (46%), Positives = 12/26 (46%), Gaps = 2/26 (7%) Frame = +2 Query: 725 PPPXKXPPPPPXXXXXXXPP--PPXP 796 PPP PPPP PP PP P Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPP 1734 Score = 27.5 bits (58), Expect = 2.7 Identities = 13/36 (36%), Positives = 13/36 (36%), Gaps = 2/36 (5%) Frame = +2 Query: 725 PPPXKXPPPPPXXXXXXXPP--PPXPGXGXXXXXPP 826 PP P PPP PP PP P PP Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734 Score = 25.8 bits (54), Expect = 8.3 Identities = 11/34 (32%), Positives = 13/34 (38%) Frame = +1 Query: 724 SPPXXTXXPPPPXXXXXSPPPPXPRXXVXXXXSP 825 S P PP P +PPPP P +P Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 26.2 bits (55), Expect = 6.3 Identities = 9/15 (60%), Positives = 9/15 (60%) Frame = +3 Query: 612 PPPPPPXXXXKXXPP 656 PPPPPP K PP Sbjct: 1882 PPPPPPMALPKAGPP 1896 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 22.6 bits (46), Expect(2) = 7.4 Identities = 7/10 (70%), Positives = 7/10 (70%) Frame = +3 Query: 600 PXRXPPPPPP 629 P PPPPPP Sbjct: 1091 PSAVPPPPPP 1100 Score = 21.4 bits (43), Expect(2) = 7.4 Identities = 7/11 (63%), Positives = 7/11 (63%) Frame = +3 Query: 612 PPPPPPXXXXK 644 PPPPPP K Sbjct: 1097 PPPPPPAEVEK 1107 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 25.8 bits (54), Expect = 8.3 Identities = 15/53 (28%), Positives = 15/53 (28%) Frame = +2 Query: 725 PPPXKXPPPPPXXXXXXXPPPPXPGXGXXXXXPPXXXXXXXXXXXXXXXXPPP 883 P P PP P PP P P G PP PPP Sbjct: 1162 PKPSVAAPPVPAPSSGI-PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,870,066 Number of Sequences: 5004 Number of extensions: 25892 Number of successful extensions: 238 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 39 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 450492750 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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