BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_N14
(895 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.055
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.13
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.29
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.6
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 6.3
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 23 7.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 8.3
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.055
Identities = 18/55 (32%), Positives = 19/55 (34%), Gaps = 4/55 (7%)
Frame = +3
Query: 606 RXPPPPPPXXXXKXXPP----XXXXGLXXXPXXXXXXKXPXXXLPPXXNXXPPPP 758
R PPPPPP + PP L P LPP PPPP
Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPP 364
Score = 28.3 bits (60), Expect = 1.6
Identities = 29/133 (21%), Positives = 32/133 (24%), Gaps = 2/133 (1%)
Frame = +2
Query: 434 PPVXSGAGXGSXLXPRG*XXLPPXXXXXXXEXTXXPKXXXKGPXPXXXXXXXSXFXXKGP 613
PP+ +G+ S P PP P P P S P
Sbjct: 325 PPIGNGSSNSSLPPP------PPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378
Query: 614 XPPSSXXXXKXXXXPXXXG--AXXXPPXGXXXXXXXXXXPPPXKXPPPPPXXXXXXXPPP 787
S P G A PP G P P PP P PP
Sbjct: 379 LSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPS 438
Query: 788 PXPGXGXXXXXPP 826
G PP
Sbjct: 439 LPMGAPAAPPLPP 451
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.9 bits (69), Expect = 0.13
Identities = 16/48 (33%), Positives = 16/48 (33%)
Frame = +2
Query: 683 PPXGXXXXXXXXXXPPPXKXPPPPPXXXXXXXPPPPXPGXGXXXXXPP 826
PP P P PPP P PPPP PG PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAP-IMGGPPPPPPPPGVAGAGPPPP 778
Score = 31.5 bits (68), Expect = 0.17
Identities = 13/24 (54%), Positives = 13/24 (54%)
Frame = +2
Query: 725 PPPXKXPPPPPXXXXXXXPPPPXP 796
PPP PPPPP PPPP P
Sbjct: 761 PPP---PPPPPGVAGAGPPPPPPP 781
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +2
Query: 728 PPXKXPPPPPXXXXXXXPPPPXP 796
PP PPP PPPP P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 30.7 bits (66), Expect = 0.29
Identities = 12/27 (44%), Positives = 12/27 (44%)
Frame = +2
Query: 725 PPPXKXPPPPPXXXXXXXPPPPXPGXG 805
PP PPPPP PPP P G
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPG 31
Score = 23.8 bits (49), Expect(2) = 1.2
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = +3
Query: 612 PPPPPPXXXXKXXPP 656
PPPPPP PP
Sbjct: 11 PPPPPPGFEPPSQPP 25
Score = 23.0 bits (47), Expect(2) = 1.2
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +3
Query: 600 PXRXPPPPPP 629
P PPPPPP
Sbjct: 6 PGNPPPPPPP 15
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.6
Identities = 12/26 (46%), Positives = 12/26 (46%), Gaps = 2/26 (7%)
Frame = +2
Query: 725 PPPXKXPPPPPXXXXXXXPP--PPXP 796
PPP PPPP PP PP P
Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/36 (36%), Positives = 13/36 (36%), Gaps = 2/36 (5%)
Frame = +2
Query: 725 PPPXKXPPPPPXXXXXXXPP--PPXPGXGXXXXXPP 826
PP P PPP PP PP P PP
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/34 (32%), Positives = 13/34 (38%)
Frame = +1
Query: 724 SPPXXTXXPPPPXXXXXSPPPPXPRXXVXXXXSP 825
S P PP P +PPPP P +P
Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 6.3
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = +3
Query: 612 PPPPPPXXXXKXXPP 656
PPPPPP K PP
Sbjct: 1882 PPPPPPMALPKAGPP 1896
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 22.6 bits (46), Expect(2) = 7.4
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +3
Query: 600 PXRXPPPPPP 629
P PPPPPP
Sbjct: 1091 PSAVPPPPPP 1100
Score = 21.4 bits (43), Expect(2) = 7.4
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = +3
Query: 612 PPPPPPXXXXK 644
PPPPPP K
Sbjct: 1097 PPPPPPAEVEK 1107
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.8 bits (54), Expect = 8.3
Identities = 15/53 (28%), Positives = 15/53 (28%)
Frame = +2
Query: 725 PPPXKXPPPPPXXXXXXXPPPPXPGXGXXXXXPPXXXXXXXXXXXXXXXXPPP 883
P P PP P PP P P G PP PPP
Sbjct: 1162 PKPSVAAPPVPAPSSGI-PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,870,066
Number of Sequences: 5004
Number of extensions: 25892
Number of successful extensions: 238
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 39
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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