Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_N05
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 267 2e-72
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 152 7e-38
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 151 1e-37
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 134 2e-32
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 83 5e-17
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 83 7e-17
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 71 2e-13
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 54 4e-08
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.38
SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase Trp4|S... 27 3.5
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.2
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 6.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 267 bits (654), Expect = 2e-72
Identities = 121/131 (92%), Positives = 126/131 (96%)
Frame = +1
Query: 238 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 417
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 418 VLLTEAPLNPKXNREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHT 597
LLTEAPLNPK NREKMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HT
Sbjct: 103 CLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHT 162
Query: 598 VPIYEGYALAH 630
VPIYEGYAL H
Sbjct: 163 VPIYEGYALPH 173
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +3
Query: 114 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 236
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
Score = 64.1 bits (149), Expect = 3e-11
Identities = 31/46 (67%), Positives = 33/46 (71%)
Frame = +2
Query: 740 VRDIKEKLCYVALXFXQEMXXXXXXXXLXKSYEXPDGQVITIGXKR 877
VRDIKEKLCYVAL F QE+ L KSYE PDGQVITIG +R
Sbjct: 209 VRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNER 254
Score = 41.9 bits (94), Expect = 1e-04
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = +3
Query: 648 DLAGRXLTDYLMKILTEXGXSFTTT 722
DLAGR LTDYLMKIL E G +F+TT
Sbjct: 179 DLAGRDLTDYLMKILMERGYTFSTT 203
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 152 bits (368), Expect = 7e-38
Identities = 69/130 (53%), Positives = 96/130 (73%), Gaps = 1/130 (0%)
Frame = +1
Query: 238 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEH 414
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + ++ L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 415 PVLLTEAPLNPKXNREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 594
P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166
Query: 595 TVPIYEGYAL 624
+VPIY+G+++
Sbjct: 167 SVPIYDGFSI 176
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +3
Query: 120 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 233
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
Score = 41.5 bits (93), Expect = 2e-04
Identities = 19/43 (44%), Positives = 24/43 (55%)
Frame = +2
Query: 740 VRDIKEKLCYVALXFXQEMXXXXXXXXLXKSYEXPDGQVITIG 868
VR+IKEK CYVA F E+ +Y+ PD Q IT+G
Sbjct: 214 VREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLG 256
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 151 bits (367), Expect = 1e-37
Identities = 65/125 (52%), Positives = 94/125 (75%)
Frame = +1
Query: 259 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 438
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 439 LNPKXNREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGY 618
+NP NREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169
Query: 619 ALAHV 633
L H+
Sbjct: 170 VLNHL 174
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +3
Query: 126 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 224
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
Score = 40.7 bits (91), Expect = 3e-04
Identities = 18/46 (39%), Positives = 27/46 (58%)
Frame = +2
Query: 740 VRDIKEKLCYVALXFXQEMXXXXXXXXLXKSYEXPDGQVITIGXKR 877
VR++KEKLCYV+ + L ++Y PDG+VI +G +R
Sbjct: 209 VREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSER 254
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 648 DLAGRXLTDYLMKILTEXGXSFTTT 722
D+AGR T YL+ +L G +F T
Sbjct: 179 DVAGRDATRYLISLLLRKGYAFNRT 203
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 134 bits (324), Expect = 2e-32
Identities = 70/161 (43%), Positives = 97/161 (60%), Gaps = 12/161 (7%)
Frame = +1
Query: 178 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 345
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 346 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKXNREKMTQIMFETFNSPAMYVAIQA 525
WD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+FN +Y+A+QA
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQA 161
Query: 526 VLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYAL 624
VL+L AS + TG V+DSGDGV+H +P+ EGY +
Sbjct: 162 VLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVI 202
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +3
Query: 132 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 215
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 83.0 bits (196), Expect = 5e-17
Identities = 39/107 (36%), Positives = 59/107 (55%)
Frame = +1
Query: 304 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKXNREKMTQIMF 483
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 484 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 624
E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +3
Query: 117 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 290
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 82.6 bits (195), Expect = 7e-17
Identities = 40/125 (32%), Positives = 74/125 (59%), Gaps = 3/125 (2%)
Frame = +1
Query: 250 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 426
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 427 TEAPLNPKXNREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTV 600
TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH +
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180
Query: 601 PIYEG 615
P+ G
Sbjct: 181 PVLNG 185
Score = 36.7 bits (81), Expect = 0.004
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +3
Query: 102 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 227
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 71.3 bits (167), Expect = 2e-13
Identities = 35/121 (28%), Positives = 65/121 (53%), Gaps = 2/121 (1%)
Frame = +1
Query: 259 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 432
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 433 APLNPKXNREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYE 612
NP+ R+++ + FE + PA Y+ QAV +A+ ++T +++D G + P+ +
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVD 168
Query: 613 G 615
G
Sbjct: 169 G 169
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +3
Query: 117 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 218
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 53.6 bits (123), Expect = 4e-08
Identities = 31/103 (30%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +1
Query: 319 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKXNREKMTQIMFE 486
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 487 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 615
P +A + ++ LYA G G+V+D G + PI +G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.38
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +1
Query: 379 FYNELRVAPEEHPVLLTE--APLNPKXNREKMTQIMFETFNSPAM---YVAIQ 522
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase
Trp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 354
Score = 27.1 bits (57), Expect = 3.5
Identities = 24/87 (27%), Positives = 30/87 (34%)
Frame = +1
Query: 400 APEEHPVLLTEAPLNPKXNREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 579
AP HP L AP+ + + ++ N Y I V L +L G
Sbjct: 166 APMCHPTLKNVAPIRKQLGLPTIFNLVGPLLNPIPTYARIIGVSKLSLGEVVAKTLLKLG 225
Query: 580 DGVSHTVPIYEGYALAHVHPPXWTWLV 660
G S V EG P TWLV
Sbjct: 226 AGRSLVVCGEEGLDEISPAGPTHTWLV 252
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -1
Query: 342 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 229
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 6.2
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 421 LLTEAPLNPKXNREKMTQIMFETFN 495
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,898,906
Number of Sequences: 5004
Number of extensions: 55498
Number of successful extensions: 182
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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