Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_N03
(858 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4 f... 138 4e-33
At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4 f... 136 2e-32
At2g24170.1 68415.m02888 endomembrane protein 70, putative simil... 131 5e-31
At3g13772.1 68416.m01738 endomembrane protein 70, putative TM4 f... 129 3e-30
At1g55130.1 68414.m06296 endomembrane protein 70, putative simil... 128 5e-30
At1g14670.1 68414.m01744 endomembrane protein 70, putative simil... 65 7e-11
At2g01970.1 68415.m00132 endomembrane protein 70, putative 63 2e-10
At5g35160.1 68418.m04167 endomembrane protein 70, putative p76, ... 53 1e-09
At5g37310.1 68418.m04481 endomembrane protein 70, putative multi... 56 4e-08
At1g10950.1 68414.m01257 endomembrane protein 70, putative 50 1e-06
At2g34180.1 68415.m04183 CBL-interacting protein kinase 13 (CIPK... 31 0.98
At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor, pu... 28 6.9
>At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4
family;
Length = 644
Score = 138 bits (334), Expect = 4e-33
Identities = 75/189 (39%), Positives = 111/189 (58%), Gaps = 11/189 (5%)
Frame = +1
Query: 229 SYGFYVPGVAPVEFKKGQKIEVKAVKMTSIHTQLPYEYYSLPLCLPKNGTFVYKSENLGE 408
++ FY+PGVAP +F+KG +++VK K+TSI TQLPY YYSLP C PK V +ENLGE
Sbjct: 25 AHSFYLPGVAPQDFEKGDELKVKVNKLTSIKTQLPYSYYSLPFCRPKK--IVDSTENLGE 82
Query: 409 VLRGDRIVNTPYEVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNL 588
VLRGDRI N PY M E C +L + + ++ +I+ EY V++++DNL
Sbjct: 83 VLRGDRIENAPYSFKMREAQMCNVLGR-----VMLDAKSAKAFKEKIDDEYRVNMILDNL 137
Query: 589 PVATKVINVETLERTIEQGYRLGY----------MSEGKAYINNHXXXXXKYHKHSQ-DS 735
P+ + ++ + + Y+LGY E K +++NH +YH+ Q D+
Sbjct: 138 PLVVPIERIDPGQGSPSVVYQLGYHVGLKGQYEGSKEQKYFMHNHLAFTVRYHRDMQTDA 197
Query: 736 YRVVGFEVE 762
R+VGFEV+
Sbjct: 198 ARIVGFEVK 206
>At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4
family;
Length = 648
Score = 136 bits (329), Expect = 2e-32
Identities = 74/187 (39%), Positives = 110/187 (58%), Gaps = 9/187 (4%)
Frame = +1
Query: 229 SYGFYVPGVAPVEFKKGQKIEVKAVKMTSIHTQLPYEYYSLPLCLPKNGTFVYKSENLGE 408
++ FY+PGVAP +F+KG +++VK K+TSI TQLPY YYSLP C P V +ENLGE
Sbjct: 31 AHSFYLPGVAPQDFEKGDELKVKVNKLTSIKTQLPYSYYSLPFCRPSK--IVDSTENLGE 88
Query: 409 VLRGDRIVNTPYEVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNL 588
VLRGDRI N PY M E C +L + + ++ +I+ EY V++++DNL
Sbjct: 89 VLRGDRIENAPYSFKMREAQMCNILGR-----VTLDAKTAKAFKEKIDDEYRVNMILDNL 143
Query: 589 PVATKVINVETLERTI--EQGYRLGY------MSEGKAYINNHXXXXXKYHKHSQ-DSYR 741
P+ + V+ ++ + GY +G E K +++NH +YH+ Q D+ R
Sbjct: 144 PLVVPIERVDQGSPSVVYQLGYHVGLKGQYEGSKEQKFFMHNHLAFTVRYHRDIQTDAAR 203
Query: 742 VVGFEVE 762
+VGFEV+
Sbjct: 204 IVGFEVK 210
>At2g24170.1 68415.m02888 endomembrane protein 70, putative similar
to MURA transposase of maize Mutator transposon
Length = 637
Score = 131 bits (317), Expect = 5e-31
Identities = 74/185 (40%), Positives = 107/185 (57%), Gaps = 8/185 (4%)
Frame = +1
Query: 232 YGFYVPGVAPVEFKKGQKIEVKAVKMTSIHTQLPYEYYSLPLCLPKNGTFVYKSENLGEV 411
+GFY+PGVAP +F+ G + VK K+TS TQLPY YYSLP C P++ V +ENLGEV
Sbjct: 22 HGFYLPGVAPQDFQMGDALMVKVNKLTSTKTQLPYSYYSLPYCRPEH--IVDSAENLGEV 79
Query: 412 LRGDRIVNTPYEVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNLP 591
LRGDRI N+P+ M E+ C +C + + ++ +I EY V++++DNLP
Sbjct: 80 LRGDRIENSPFVFKMRESQMCAAVCR-----VKLDKKTAKAFKEKIADEYRVNMILDNLP 134
Query: 592 VATKVINVETLERTIEQ-GYRLGY------MSEGKAYINNHXXXXXKYHKHSQ-DSYRVV 747
+ V + + Q G+ +G E K +I+NH +YH+ Q DS R+V
Sbjct: 135 LVVPVQRPDQDNVVVYQHGFHVGLKGIFAGKKEEKYFIHNHLTFTVRYHRDIQTDSSRIV 194
Query: 748 GFEVE 762
GFEV+
Sbjct: 195 GFEVK 199
>At3g13772.1 68416.m01738 endomembrane protein 70, putative TM4
family;
Length = 641
Score = 129 bits (311), Expect = 3e-30
Identities = 73/185 (39%), Positives = 106/185 (57%), Gaps = 8/185 (4%)
Frame = +1
Query: 229 SYGFYVPGVAPVEFKKGQKIEVKAVKMTSIHTQLPYEYYSLPLCLPKNGTFVYKSENLGE 408
S FY+PGVAP +F+KG + VK K++S TQLPY+YY L C P + +ENLGE
Sbjct: 25 SRAFYLPGVAPRDFQKGDPLYVKVNKLSSTKTQLPYDYYYLNYCKPPK--ILNNAENLGE 82
Query: 409 VLRGDRIVNTPYEVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNL 588
VLRGDRI N+ Y M E+ CK+ C + + + ++ +I+ EY ++++DNL
Sbjct: 83 VLRGDRIENSVYTFQMLEDQPCKVGCR-----VKLNADSTKNFKEKIDDEYRANMILDNL 137
Query: 589 PVAT-KVINVETLERTIEQGYRLGY------MSEGKAYINNHXXXXXKYHKHSQ-DSYRV 744
PVA + + T E G+R+G+ E K +I+NH YH+ + DS R+
Sbjct: 138 PVAVLRQRRDGSQSTTYEHGFRVGFKGSYEGSKEEKYFIHNHLSFRVMYHRDQESDSARI 197
Query: 745 VGFEV 759
VGFEV
Sbjct: 198 VGFEV 202
>At1g55130.1 68414.m06296 endomembrane protein 70, putative similar
to multispanning membrane protein GI:2276460 from [Homo
sapiens]
Length = 637
Score = 128 bits (309), Expect = 5e-30
Identities = 71/184 (38%), Positives = 106/184 (57%), Gaps = 8/184 (4%)
Frame = +1
Query: 232 YGFYVPGVAPVEFKKGQKIEVKAVKMTSIHTQLPYEYYSLPLCLPKNGTFVYKSENLGEV 411
+ FY+PGVAP +F+KG + VK K++S TQLPY++Y L C P + ENLGEV
Sbjct: 22 HAFYLPGVAPRDFQKGDPLYVKVNKLSSTKTQLPYDFYYLNYCKPPK--ILNTGENLGEV 79
Query: 412 LRGDRIVNTPYEVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNLP 591
LRGDRI N+ Y M E+ C++ C + E ++ +I++EY ++++DNLP
Sbjct: 80 LRGDRIENSVYTFEMLEDQPCRVGCR-----VRVDAESAKNFREKIDYEYRANMILDNLP 134
Query: 592 VATKVINVETLE-RTIEQGYRLGY------MSEGKAYINNHXXXXXKYHKHSQ-DSYRVV 747
VA + ++ T E GYR+G+ E K +I+NH YH+ + +S R+V
Sbjct: 135 VAVLRQRKDGIQSTTYEHGYRVGFKGSYEGSKEKKYFIHNHLSFRVMYHRDQESESSRIV 194
Query: 748 GFEV 759
GFEV
Sbjct: 195 GFEV 198
>At1g14670.1 68414.m01744 endomembrane protein 70, putative similar
to endomembrane protein emp70 precursor isolog
GB:AAF67014 GI:7677068 (Homo sapiens)
Length = 592
Score = 64.9 bits (151), Expect = 7e-11
Identities = 33/110 (30%), Positives = 60/110 (54%), Gaps = 1/110 (0%)
Frame = +1
Query: 268 FKKGQKIEVKAVKMTSIHTQLP-YEYYSLPLCLPKNGTFVYKSENLGEVLRGDRIVNTPY 444
+K+G + + A K+ H Y Y+ LP C+P+ K E LGEVL GDR+V+ PY
Sbjct: 31 YKEGDTVPLYANKVGPFHNPSETYRYFDLPFCIPEG--VKEKKEALGEVLNGDRLVSAPY 88
Query: 445 EVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNLPV 594
+++ + ++ C++K S E+ ++ +E +Y+ + D+LP+
Sbjct: 89 KLNFRDEKESEVYCNKK-----LSKEEVKQFRKAVEKDYYFQMYYDDLPI 133
>At2g01970.1 68415.m00132 endomembrane protein 70, putative
Length = 592
Score = 63.3 bits (147), Expect = 2e-10
Identities = 34/110 (30%), Positives = 57/110 (51%), Gaps = 1/110 (0%)
Frame = +1
Query: 268 FKKGQKIEVKAVKMTSIHTQLP-YEYYSLPLCLPKNGTFVYKSENLGEVLRGDRIVNTPY 444
+K G + + A K+ H Y Y+ LP C+P+ K E LGEVL GDR+V+ PY
Sbjct: 31 YKDGDSVPLYANKVGPFHNPSETYRYFDLPFCIPEG--VKDKKEALGEVLNGDRLVSAPY 88
Query: 445 EVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNLPV 594
+++ + ++ C +K S E+ E +E +Y+ + D+LP+
Sbjct: 89 KLNFRDEKDSEVYCKKK-----LSREEVEHFRRAVEKDYYFQMYYDDLPI 133
>At5g35160.1 68418.m04167 endomembrane protein 70, putative p76,
Homo sapiens, EMBL:HSU81006
Length = 627
Score = 53.2 bits (122), Expect(2) = 1e-09
Identities = 35/113 (30%), Positives = 53/113 (46%)
Frame = +1
Query: 391 SENLGEVLRGDRIVNTPYEVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVH 570
+ENLGE+L GDRI N+PY M +N LC + S + + + RI+ Y V+
Sbjct: 43 AENLGELLMGDRIENSPYRFRMFKNESEIFLCQTDKL----SADSLKLLKKRIDEMYQVN 98
Query: 571 LLVDNLPVATKVINVETLERTIEQGYRLGYMSEGKAYINNHXXXXXKYHKHSQ 729
++DNLP + R GY +G + Y+ NH HK+ +
Sbjct: 99 PMLDNLPAIRYTKRDGYVLRW--TGYPVGIKVQDVYYVFNHLKFKVLVHKYEE 149
Score = 27.5 bits (58), Expect(2) = 1e-09
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 229 SYGFYVPGVAPVEFKKGQKIEVK 297
S+GFY+PG P +++ G + VK
Sbjct: 18 SFGFYLPGSYPHKYEVGDYLNVK 40
>At5g37310.1 68418.m04481 endomembrane protein 70, putative
multispanning membrane protein, Homo sapiens,
EMBL:HSU94831
Length = 564
Score = 55.6 bits (128), Expect = 4e-08
Identities = 43/152 (28%), Positives = 68/152 (44%), Gaps = 1/152 (0%)
Frame = +1
Query: 268 FKKGQKIEVKAVKMTSIHTQLP-YEYYSLPLCLPKNGTFVYKSENLGEVLRGDRIVNTPY 444
+K G + + A K+ H Y Y+ LP C + K E LGEVL GDR+V+ PY
Sbjct: 32 YKVGDDVPLYANKVGPFHNPSETYRYFDLPFC--SSAPVKEKKEALGEVLNGDRLVSAPY 89
Query: 445 EVHMAENIXCKLLCHRKNVPINWSVEDSEKVASRIEHEYFVHLLVDNLPVATKVINVETL 624
++ ++ C ++ S ED K I +Y+ + D+LP+ L
Sbjct: 90 KLEFLGEKNSEVACRKR-----LSREDVAKFRDVIAKDYYFQMYYDDLPI------WGFL 138
Query: 625 ERTIEQGYRLGYMSEGKAYINNHXXXXXKYHK 720
+ +++G SE K Y+ NH Y+K
Sbjct: 139 GKVVKEGKT--DPSEYKYYLFNHLQFEIFYNK 168
>At1g10950.1 68414.m01257 endomembrane protein 70, putative
Length = 589
Score = 50.4 bits (115), Expect = 1e-06
Identities = 27/90 (30%), Positives = 45/90 (50%)
Frame = +1
Query: 325 QLPYEYYSLPLCLPKNGTFVYKSENLGEVLRGDRIVNTPYEVHMAENIXCKLLCHRKNVP 504
Q Y YYSLP C P +G V+K LGEVL G+ ++++ + +N+ ++C P
Sbjct: 51 QETYNYYSLPFCRP-SGNNVHKWGGLGEVLGGNELIDSEIAIKFMKNVERSVIC-----P 104
Query: 505 INWSVEDSEKVASRIEHEYFVHLLVDNLPV 594
+ + IE Y+ +D+LP+
Sbjct: 105 LELDEAKVKHFKDAIESSYWFEFFMDDLPL 134
>At2g34180.1 68415.m04183 CBL-interacting protein kinase 13 (CIPK13)
identical to CBL-interacting protein kinase 13
[Arabidopsis thaliana] gi|13249125|gb|AAK16688
Length = 502
Score = 31.1 bits (67), Expect = 0.98
Identities = 31/125 (24%), Positives = 54/125 (43%), Gaps = 4/125 (3%)
Frame = +1
Query: 244 VPGVAPVEFKKGQKIEVKAVKMTSIHTQLPYEYYSLPLCLPKNGTFVYKSENLGEVLRGD 423
+PG P++F G + K T+ T P S P P+ + K E +G++L
Sbjct: 11 IPGPTPIQFMAGLLARI-VTKNTNKETSTPESPRS-PRT-PQGSILMDKYE-IGKLLGHG 66
Query: 424 RI--VNTPYEVHMAENIXCKLLCHRKNVPINWS--VEDSEKVASRIEHEYFVHLLVDNLP 591
V +H E++ K++ K V + ++ + R+ H Y VHLL + +
Sbjct: 67 SFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKREISILRRVRHPYIVHLL-EVMA 125
Query: 592 VATKV 606
TK+
Sbjct: 126 TKTKI 130
>At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor,
putative (AVDE1) similar to EST gb|N37612
Length = 462
Score = 28.3 bits (60), Expect = 6.9
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = +1
Query: 490 RKNVPINWSVEDSEKVASRIEHEYFVHLLVDNLPVATKVINVETLERTIEQGYRL 654
R +V N + + EK A I ++ + DN + VE +E+T+E+G R+
Sbjct: 329 RSSVLPNSIIPELEKAAKSIGRDFSTFIRTDNTCGPEPAL-VERIEKTVEEGERI 382
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,610,816
Number of Sequences: 28952
Number of extensions: 299950
Number of successful extensions: 645
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 600
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 623
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1999652000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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