Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_N02
(853 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 67 1e-11
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 67 2e-11
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 65 5e-11
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 50 2e-06
At2g45630.2 68415.m05674 oxidoreductase family protein low simil... 48 1e-05
At1g12550.1 68414.m01455 oxidoreductase family protein similar t... 46 2e-05
At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical t... 41 0.001
At1g72190.1 68414.m08347 oxidoreductase family protein similar t... 38 0.008
At5g25550.1 68418.m03040 leucine-rich repeat family protein / ex... 27 1.3
At1g01510.1 68414.m00067 C-terminal binding protein (ANGUSTIFOLI... 28 6.9
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 67.3 bits (157), Expect = 1e-11
Identities = 40/76 (52%), Positives = 46/76 (60%)
Frame = +1
Query: 286 RGGLIQETDFLXALKSGKVGGAALDVFEXEPPTDPVTLEIIQQPAVIATPHLGASTKEAQ 465
RGG+I E + AL +G V AALDVF EPP+ L IQ V TPHLGASTKEAQ
Sbjct: 312 RGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRL--IQHENVTVTPHLGASTKEAQ 369
Query: 466 VRVGQEIAEQLVNLVK 513
V EIAE + +K
Sbjct: 370 EGVAIEIAEAVAGALK 385
Score = 41.5 bits (93), Expect = 7e-04
Identities = 18/63 (28%), Positives = 30/63 (47%)
Frame = +3
Query: 93 IGFDPFVSADQCAQFHCTKMELEDXWPLADYITLHTPXIESTRNFINADVLKXCXKGVKI 272
I DP+ AD+ + + AD+++LH P +T+ N + KGV++
Sbjct: 248 ISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRL 307
Query: 273 INV 281
INV
Sbjct: 308 INV 310
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 66.9 bits (156), Expect = 2e-11
Identities = 37/76 (48%), Positives = 47/76 (61%)
Frame = +1
Query: 286 RGGLIQETDFLXALKSGKVGGAALDVFEXEPPTDPVTLEIIQQPAVIATPHLGASTKEAQ 465
RGG+I E L AL SG V AALDVF EPP +++ +V ATPHLGAST EAQ
Sbjct: 276 RGGVIDEEALLRALDSGIVAQAALDVFTVEPPVK--DNKLVLHESVTATPHLGASTMEAQ 333
Query: 466 VRVGQEIAEQLVNLVK 513
V E+AE ++ ++
Sbjct: 334 EGVSIEVAEAVIGALR 349
Score = 38.7 bits (86), Expect = 0.005
Identities = 20/63 (31%), Positives = 29/63 (46%)
Frame = +3
Query: 93 IGFDPFVSADQCAQFHCTKMELEDXWPLADYITLHTPXIESTRNFINADVLKXCXKGVKI 272
I DP+ AD+ + E AD+I+LH P +T +N KGV+I
Sbjct: 212 ITHDPYAPADRARAIGVELVSFEVAISTADFISLHLPLTAATSKMMNDVTFAMMKKGVRI 271
Query: 273 INV 281
+NV
Sbjct: 272 VNV 274
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 65.3 bits (152), Expect = 5e-11
Identities = 38/72 (52%), Positives = 43/72 (59%)
Frame = +1
Query: 286 RGGLIQETDFLXALKSGKVGGAALDVFEXEPPTDPVTLEIIQQPAVIATPHLGASTKEAQ 465
RGG+I E + AL +G V AALDVF EPP L +Q V TPHLGAST EAQ
Sbjct: 291 RGGVIDEDALVRALDAGIVAQAALDVFTKEPPAKDSKL--VQHERVTVTPHLGASTMEAQ 348
Query: 466 VRVGQEIAEQLV 501
V EIAE +V
Sbjct: 349 EGVAIEIAEAVV 360
Score = 43.2 bits (97), Expect = 2e-04
Identities = 19/65 (29%), Positives = 32/65 (49%)
Frame = +3
Query: 87 KXIGFDPFVSADQCAQFHCTKMELEDXWPLADYITLHTPXIESTRNFINADVLKXCXKGV 266
+ I DP+ AD+ + ++ AD+I+LH P +T +N + KGV
Sbjct: 225 RVIAHDPYAPADRAHAIGVDLVSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGV 284
Query: 267 KIINV 281
+I+NV
Sbjct: 285 RIVNV 289
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 50.0 bits (114), Expect = 2e-06
Identities = 26/61 (42%), Positives = 36/61 (59%)
Frame = +1
Query: 283 GRGGLIQETDFLXALKSGKVGGAALDVFEXEPPTDPVTLEIIQQPAVIATPHLGASTKEA 462
GRG + E + + AL G++GGAALDVFE EP V E+ V+ PH+G+ T E
Sbjct: 231 GRGPHVDEQELIKALTEGRLGGAALDVFEQEP---HVPEELFGLENVVLLPHVGSGTVET 287
Query: 463 Q 465
+
Sbjct: 288 R 288
>At2g45630.2 68415.m05674 oxidoreductase family protein low
similarity to SP|P36234 Glycerate dehydrogenase (EC
1.1.1.29) (NADH-dependent hydroxypyruvate reductase)
{Hyphomicrobium methylovorum}; contains Pfam profile
PF00389: D-isomer specific 2-hydroxyacid dehydrogenase,
catalytic domain
Length = 338
Score = 47.6 bits (108), Expect = 1e-05
Identities = 24/67 (35%), Positives = 37/67 (55%)
Frame = +1
Query: 286 RGGLIQETDFLXALKSGKVGGAALDVFEXEPPTDPVTLEIIQQPAVIATPHLGASTKEAQ 465
RG +I E + + L+ G++GGA LDVFE EP V E+ + V+ +PH T E
Sbjct: 257 RGAIIDEEEMVRCLREGEIGGAGLDVFEDEP---NVPKELFELDNVVFSPHSAFMTLEGL 313
Query: 466 VRVGQEI 486
+G+ +
Sbjct: 314 EELGKVV 320
>At1g12550.1 68414.m01455 oxidoreductase family protein similar to
glyoxylate reductase from Homo sapiens (gi:6002730);
contains Pfam D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain PF02826
Length = 323
Score = 46.4 bits (105), Expect = 2e-05
Identities = 26/66 (39%), Positives = 34/66 (51%)
Frame = +1
Query: 283 GRGGLIQETDFLXALKSGKVGGAALDVFEXEPPTDPVTLEIIQQPAVIATPHLGASTKEA 462
GRG LI E + + L G +GGA LDVFE EP V E+ V+ +PH +T +
Sbjct: 239 GRGKLIDEKEMVKCLVDGVIGGAGLDVFENEP---AVPQELFGLDNVVLSPHFAVATPGS 295
Query: 463 QVRVGQ 480
V Q
Sbjct: 296 LDNVAQ 301
>At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical to
GI:7677266
Length = 384
Score = 40.7 bits (91), Expect = 0.001
Identities = 23/82 (28%), Positives = 40/82 (48%)
Frame = +1
Query: 286 RGGLIQETDFLXALKSGKVGGAALDVFEXEPPTDPVTLEIIQQPAVIATPHLGASTKEAQ 465
RG +++ + A++SG +GG + DV++ +P + A+ TPH +T +AQ
Sbjct: 290 RGAIMERQAVVDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAM--TPHTSGTTIDAQ 347
Query: 466 VRVGQEIAEQLVNLVKPGTFPT 531
+R + L K FPT
Sbjct: 348 LRYAAGTKDMLERYFKGEDFPT 369
Score = 33.1 bits (72), Expect = 0.24
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +3
Query: 153 ELEDXWPLADYITLHTPXIESTRNFINADVLKXCXKGVKIIN 278
+L + P D I ++ P E TR N +++ KGV I+N
Sbjct: 246 DLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVN 287
>At1g72190.1 68414.m08347 oxidoreductase family protein similar to
D-3-phosphoglycerate dehydrogenase from Arabidopsis
thaliana [SP|O04130], glyoxylate reductase from Homo
sapiens (gi:6002730); contains Pfam D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain PF02826
Length = 373
Score = 37.9 bits (84), Expect = 0.008
Identities = 24/59 (40%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +1
Query: 286 RGGLIQETDFLXALKSGKVGGAALDVFEXEP--PTDPVTLEIIQQPAVIATPHLGASTK 456
RGGLI L+SG +GG +DV EP P DP I++ VI TPH+ T+
Sbjct: 289 RGGLINYESAFQNLESGHLGGLGIDVAWSEPFDPNDP----ILKFKNVIITPHVAGVTE 343
>At5g25550.1 68418.m03040 leucine-rich repeat family protein /
extensin family protein similar to leucine-rich
repeat/extensin 1 (GI:13809918) [Arabidopsis thaliana];
contains Pfam PF00560: Leucine Rich Repeat domains
Length = 433
Score = 27.5 bits (58), Expect(2) = 1.3
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -1
Query: 541 RPVESGMFPASPGSRAAQQSPGQLSP 464
+PV+ F SPGS P Q+SP
Sbjct: 364 KPVDCDSFKCSPGSSCFSPPPSQISP 389
Score = 21.8 bits (44), Expect(2) = 1.3
Identities = 9/23 (39%), Positives = 10/23 (43%)
Frame = -1
Query: 382 SAAPARTRPEQPRPPFQTSEPAR 314
S+ P P PP T PAR
Sbjct: 390 SSQPLAPAPSPTSPPLSTPPPAR 412
>At1g01510.1 68414.m00067 C-terminal binding protein (ANGUSTIFOLIA)
nearly identical to C-terminal binding protein
ANGUSTIFOLIA [Arabidopsis thaliana] GI:15408535;
contains Pfam profile PF02826: D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain
Length = 636
Score = 28.3 bits (60), Expect = 6.9
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = +3
Query: 156 LEDXWPLADYITLHTPXIESTRNFINADVLKXCXKGVKIINVG 284
L D +D I+LH T +NA+ L+ G ++N G
Sbjct: 217 LNDLLAASDVISLHCALTNDTVQILNAECLQHIKPGAFLVNTG 259
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,928,214
Number of Sequences: 28952
Number of extensions: 183645
Number of successful extensions: 478
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 455
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 472
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1980143200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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