Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_M23
(881 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 34 0.031
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 33 0.054
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 31 0.29
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 30 0.38
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.38
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.50
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 29 0.88
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.2
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 28 1.5
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.7
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 2.7
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 4.7
SPBC1709.09 |||mitochondrial translation termination factor|Schi... 26 6.2
SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces... 26 6.2
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 6.2
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 8.2
SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc... 26 8.2
SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyce... 26 8.2
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 33.9 bits (74), Expect = 0.031
Identities = 32/125 (25%), Positives = 57/125 (45%)
Frame = +1
Query: 274 QDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPD 453
++++ WK +SVL N + + L + K EALE+ +Q +E +E K
Sbjct: 1329 KEYNSRWKLRFQSVL---NKYERVDPTQLEELK-KNCEALEKEKQELETKLQETAKETDT 1384
Query: 454 VEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPKIKAAYDDFAKNTQEVI 633
++ +L E+ V+N +E ++ K + NEK K++ FA QE+
Sbjct: 1385 FKQQVNSLNEE----VENLKKEVEQANTKNTRLAAAWNEKCENLKKSSLTRFAHLKQELT 1440
Query: 634 KKIQE 648
K +E
Sbjct: 1441 NKNKE 1445
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 33.1 bits (72), Expect = 0.054
Identities = 21/87 (24%), Positives = 44/87 (50%), Gaps = 3/87 (3%)
Frame = +1
Query: 280 FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPD-- 453
F + ++ + +L N + + L + +A KA+++LEQ+ + E L K H +
Sbjct: 27 FDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQTEARKQNFTELLEKEHEEQA 85
Query: 454 -VEKNATALREKLQAAVQNTVQESQKL 531
E+ + +EKL A ++ + S++L
Sbjct: 86 ITEQEIFSFQEKLDAMLKRKQKLSEEL 112
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 30.7 bits (66), Expect = 0.29
Identities = 25/68 (36%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +1
Query: 283 SKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRK-AHPDVE 459
++ + D S S +L A AK A DA A E +++ +IER+A K A + E
Sbjct: 129 ARQYNDRSTSEALELEARAKK---AAQDAE-LASERAREAQSSIERSASLREKQAREEAE 184
Query: 460 KNATALRE 483
+ ATALRE
Sbjct: 185 RAATALRE 192
>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 633
Score = 30.3 bits (65), Expect = 0.38
Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 8/80 (10%)
Frame = +1
Query: 376 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 531
K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186
Query: 532 AKKVSSNVQETNEKLAPKIK 591
K + T +KL KI+
Sbjct: 187 YKDMEQENASTIQKLTSKIR 206
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 30.3 bits (65), Expect = 0.38
Identities = 17/81 (20%), Positives = 38/81 (46%)
Frame = +1
Query: 328 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 507
N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + +
Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396
Query: 508 TVQESQKLAKKVSSNVQETNE 570
+QE ++ K + Q+ +E
Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.50
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +1
Query: 295 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 432
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 29.1 bits (62), Expect = 0.88
Identities = 17/69 (24%), Positives = 38/69 (55%)
Frame = +1
Query: 376 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 555
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185
Query: 556 QETNEKLAP 582
+ ++ ++P
Sbjct: 186 RSSSRSISP 194
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +1
Query: 274 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 429
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 28.3 bits (60), Expect = 1.5
Identities = 20/85 (23%), Positives = 45/85 (52%)
Frame = +1
Query: 391 LEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNE 570
++ Q+IE T L K D+E++ +++ + V + Q+ ++++ +Q+T E
Sbjct: 496 MKTQEQSIELT--RLYKQLQDIEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKE 553
Query: 571 KLAPKIKAAYDDFAKNTQEVIKKIQ 645
L+ K + DD+ +EV+ K++
Sbjct: 554 VLSKSSKES-DDY----EEVVGKLR 573
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 406 ASTVPKPPWPCRSRLRALPG 347
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 27.5 bits (58), Expect = 2.7
Identities = 27/108 (25%), Positives = 48/108 (44%)
Frame = +1
Query: 304 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 483
S + + A K L GA KAKE ++ R +RTA E+RK +E+ R
Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199
Query: 484 KLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPKIKAAYDDFAKNTQE 627
+ A Q + Q +++ ++ + + L +I+ A + + T E
Sbjct: 200 ERAAEAQRVAGKEQ-----LANILKHSTDLLEARIERANINISAQTSE 242
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 26.6 bits (56), Expect = 4.7
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +1
Query: 343 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 498
S++ L + N + KE +E + RT +E EKN LRE+L+ A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576
>SPBC1709.09 |||mitochondrial translation termination
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 26.2 bits (55), Expect = 6.2
Identities = 18/71 (25%), Positives = 35/71 (49%)
Frame = +1
Query: 406 QNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPK 585
+NI + E+ R + NA+ + K Q + T++ Q+LAK + +++ E+L+
Sbjct: 136 KNILKAIEDSRYPFVANKLNASTIEVKPQ---RTTLESRQQLAKVLEGYAKDSREQLSAM 192
Query: 586 IKAAYDDFAKN 618
+ AKN
Sbjct: 193 RTELKKEIAKN 203
>SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 172
Score = 26.2 bits (55), Expect = 6.2
Identities = 13/86 (15%), Positives = 41/86 (47%)
Frame = +1
Query: 382 KEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQE 561
K ++R+N++ + + P+++ + + +L+ +++++ Q + + V+E
Sbjct: 81 KRRNRKARENLKVNVSSIGEVLPEIDLDISVANSRLKPVIKDSLSSKQTKSSMKRNTVEE 140
Query: 562 TNEKLAPKIKAAYDDFAKNTQEVIKK 639
E+ +K + F N E +++
Sbjct: 141 I-ERFQAILK--HPSFVSNPLETVRE 163
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 6.2
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +1
Query: 391 LEQSRQNIERTAEELRKAHPDVEK 462
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 8.2
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
Frame = +1
Query: 538 KVSSNVQET--NEKLAPKIKAAYD 603
+V N++ET EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322
>SPAC23H4.11c |cnl2||centromere localized protein
Cnl2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 25.8 bits (54), Expect = 8.2
Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +1
Query: 382 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 555
++ L + R NI ++ + K+ D + N L+ A+ + V+E ++
Sbjct: 53 QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112
Query: 556 QETNEKLAPKIKAAY 600
E NE+L+ +++AY
Sbjct: 113 SEINERLSEAVQSAY 127
>SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 406
Score = 25.8 bits (54), Expect = 8.2
Identities = 32/139 (23%), Positives = 61/139 (43%), Gaps = 7/139 (5%)
Frame = +1
Query: 253 LTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 432
+ S D+++ S+ D ++ L+ +NA Q + K+ E L SRQ+ A+E
Sbjct: 28 IASSSDSEEESEL--DTNKQALEHINA-----QKNITHNENKSAEPL--SRQSTILDADE 78
Query: 433 LRKAHPDVEKNATAL--REKLQAAVQNTVQE-----SQKLAKKVSSNVQETNEKLAPKIK 591
+ D NA R ++A+ Q + + A + +N + L+
Sbjct: 79 GNQDVSDTTPNACLNEGRHSPKSAISCVTQPVSPVYNTRAAANLRNNSINSEAALSTTSS 138
Query: 592 AAYDDFAKNTQEVIKKIQE 648
DDFA+ +E+ +++QE
Sbjct: 139 LLDDDFARRLEEIDRQVQE 157
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,330,249
Number of Sequences: 5004
Number of extensions: 36389
Number of successful extensions: 173
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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