BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_M11
(994 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 33 0.062
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.77
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 24 2.3
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 2.3
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 7.2
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 33.1 bits (72), Expect = 0.062
Identities = 25/72 (34%), Positives = 25/72 (34%), Gaps = 3/72 (4%)
Frame = -3
Query: 854 NXVGXXXXXXGGGGRPPXXXGGGVFVSXFXXG---GGGAXCXKXPPPXXXPFXGXGGXXG 684
N G G GG PP G G F G GG P G GG G
Sbjct: 186 NGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPG 245
Query: 683 GPGGGXXGFCXG 648
G GGG GF G
Sbjct: 246 GFGGGLGGFGGG 257
Score = 27.5 bits (58), Expect = 3.1
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2
Query: 575 GGGXGXPPPXGGGF 616
GGG G PPP GGF
Sbjct: 194 GGGSGGPPPGPGGF 207
Score = 26.2 bits (55), Expect = 7.2
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +3
Query: 711 GGXXGGXGFXTXGPPPPP 764
GG GG G + GPPP P
Sbjct: 187 GGGFGGFGGGSGGPPPGP 204
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.77
Identities = 19/58 (32%), Positives = 19/58 (32%), Gaps = 2/58 (3%)
Frame = -2
Query: 738 KXPXPPXXPLXXXGGXXXGPRGGXXGILRXGAPP--SVXGXXXXPPPXGGGXPXPPPP 571
K P PP P G G L PP S PP G P PPPP
Sbjct: 309 KRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPP 366
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 24.2 bits (50), Expect(2) = 2.3
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +1
Query: 661 PXXPPPGPPXXPP 699
P PPP PP PP
Sbjct: 236 PAPPPPPPPTLPP 248
Score = 21.8 bits (44), Expect(2) = 2.3
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +1
Query: 658 NPXXPPPGPP 687
NP PPP PP
Sbjct: 188 NPPPPPPPPP 197
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.9 bits (59), Expect = 2.3
Identities = 12/25 (48%), Positives = 13/25 (52%), Gaps = 2/25 (8%)
Frame = -2
Query: 639 PSVXGXXXXPPPXG--GGXPXPPPP 571
P + G PPP G G P PPPP
Sbjct: 756 PIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 27.1 bits (57), Expect = 4.1
Identities = 12/28 (42%), Positives = 13/28 (46%), Gaps = 4/28 (14%)
Frame = -2
Query: 642 PPSVXGXXXXPPP----XGGGXPXPPPP 571
P + G PPP G G P PPPP
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 4.1
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +1
Query: 928 PPPPXFPXXGGGGXXPXPXP 987
PPPP P G G P P P
Sbjct: 762 PPPPPPPGVAGAGPPPPPPP 781
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 7.2
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +1
Query: 646 SPXQNPXXPPPGPPXXPPXPXK 711
S P PPP P PP P K
Sbjct: 142 SAPPRPSIPPPSPASAPPIPSK 163
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,903,044
Number of Sequences: 5004
Number of extensions: 25891
Number of successful extensions: 145
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 513276802
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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