BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_L22
(769 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_A5KRM5 Cluster: Putative uncharacterized protein; n=1; ... 40 0.090
UniRef50_Q8IU42 Cluster: Formin homology protein A; n=2; Dictyos... 39 0.12
UniRef50_Q9FLQ7 Cluster: Gb|AAD23008.1; n=1; Arabidopsis thalian... 37 0.48
UniRef50_Q6C9I8 Cluster: Similar to sp|P41832 Saccharomyces cere... 37 0.48
UniRef50_UPI0000E4896A Cluster: PREDICTED: similar to CG33556-PA... 37 0.63
UniRef50_Q54RW5 Cluster: Putative uncharacterized protein; n=1; ... 35 1.9
UniRef50_Q6CDQ5 Cluster: Similarity; n=2; Saccharomycetales|Rep:... 35 1.9
UniRef50_P14734 Cluster: Protein fork head; n=8; Endopterygota|R... 35 1.9
UniRef50_UPI0000498915 Cluster: hypothetical protein 9.t00033; n... 34 4.5
UniRef50_Q1ZXK2 Cluster: Actin-binding protein; n=2; Dictyosteli... 34 4.5
UniRef50_Q5C3I4 Cluster: SJCHGC03138 protein; n=2; Schistosoma j... 33 5.9
UniRef50_Q7JP75 Cluster: Cytokinesis defect protein 1, isoform b... 33 7.8
UniRef50_A5JUU8 Cluster: Formin B; n=2; Trypanosoma brucei|Rep: ... 33 7.8
UniRef50_A2DM28 Cluster: Diaphanous, putative; n=1; Trichomonas ... 33 7.8
UniRef50_A0CZ14 Cluster: Chromosome undetermined scaffold_31, wh... 33 7.8
UniRef50_Q1E467 Cluster: Putative uncharacterized protein; n=1; ... 33 7.8
>UniRef50_A5KRM5 Cluster: Putative uncharacterized protein; n=1;
Ruminococcus torques ATCC 27756|Rep: Putative
uncharacterized protein - Ruminococcus torques ATCC
27756
Length = 159
Score = 39.5 bits (88), Expect = 0.090
Identities = 36/110 (32%), Positives = 37/110 (33%), Gaps = 10/110 (9%)
Frame = -3
Query: 596 GGGGXAPPXXFFXXXKKK-----XFFFFXXXXXXPXXXXGGGPPXXXXXPPPXXXXGGXX 432
GGGG P FF KKK FFFF GGG P PPP
Sbjct: 24 GGGGGGPLFFFFFFSKKKKKRGCLFFFFFFPRGEKIFFFGGGAP----PPPPPLKKKKKI 79
Query: 431 XXXVFXXKXXGGFF*KXGAXPXXXGXPGXFXF-----FFF*PRGKXGAXP 297
F + GG F P P F F FFF RG G P
Sbjct: 80 FFFFFKKRGGGGGFFFFFPPPQQNPPPFFFFFLGFFLFFFKKRGGGGFSP 129
>UniRef50_Q8IU42 Cluster: Formin homology protein A; n=2;
Dictyostelium discoideum|Rep: Formin homology protein A
- Dictyostelium discoideum (Slime mold)
Length = 1218
Score = 39.1 bits (87), Expect = 0.12
Identities = 21/62 (33%), Positives = 21/62 (33%)
Frame = +2
Query: 476 GGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXXXXXXXXXXGGXP 655
GGGGPPPP P P G G PPPPP G P
Sbjct: 674 GGGGPPPPPPPPPMTGGGPPPPP--PPPPMTGGGPPPPPPPPGGGPPPPPPPPGAKAGGP 731
Query: 656 PP 661
PP
Sbjct: 732 PP 733
Score = 37.9 bits (84), Expect = 0.27
Identities = 21/62 (33%), Positives = 21/62 (33%)
Frame = +2
Query: 476 GGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXXXXXXXXXXGGXP 655
GGG PPPP P P G G PPPPP GG P
Sbjct: 659 GGGAPPPPPPPPPMTGGGGPPPPP-PPPPMTGGGPPPPPPPPPMTGGGPPPPPPPPGGGP 717
Query: 656 PP 661
PP
Sbjct: 718 PP 719
Score = 33.1 bits (72), Expect = 7.8
Identities = 25/86 (29%), Positives = 25/86 (29%), Gaps = 6/86 (6%)
Frame = +2
Query: 422 PXXXXXPPXXGXGGXXXXG------GGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAX 583
P PP G GG GGGPPPP P G G
Sbjct: 664 PPPPPPPPMTGGGGPPPPPPPPPMTGGGPPPPPPPPPMTGGGPPPPP-----PPPGGGPP 718
Query: 584 PPPPPXXXXXXXXXXXXXXXGGXPPP 661
PPPPP G PPP
Sbjct: 719 PPPPPPGAKAGGPPPPPPPFGKGPPP 744
>UniRef50_Q9FLQ7 Cluster: Gb|AAD23008.1; n=1; Arabidopsis
thaliana|Rep: Gb|AAD23008.1 - Arabidopsis thaliana
(Mouse-ear cress)
Length = 1289
Score = 37.1 bits (82), Expect = 0.48
Identities = 33/137 (24%), Positives = 33/137 (24%), Gaps = 4/137 (2%)
Frame = +3
Query: 264 PKKTPPXXKEGXXRXXXXXXXXXXXXXXPRAPPXXXXGPXFLKKPPXXFXXKXXXXXXPP 443
P PP G P PP P PP PP
Sbjct: 957 PPPPPPPPSYGSPPPPPPPPPGYGSPPPPPPPPPSYGSPPPPPPPPFSHVSSIPPPPPPP 1016
Query: 444 XXXGGGGXXXXX----GGAPPXPPXRXXXXXXKKKKXLFFXXXKKXXGGRXPPPPPXXXX 611
GG GGAPP PP GG PPPPP
Sbjct: 1017 PMHGGAPPPPPPPPMHGGAPPPPPPPPMHGGAPPPPP-----PPPMHGGAPPPPPPPMFG 1071
Query: 612 XXXXXXXXXXXGGXPPP 662
GG PPP
Sbjct: 1072 GAQPPPPPPMRGGAPPP 1088
>UniRef50_Q6C9I8 Cluster: Similar to sp|P41832 Saccharomyces
cerevisiae YNL271c BNI1 regulator of budding; n=1;
Yarrowia lipolytica|Rep: Similar to sp|P41832
Saccharomyces cerevisiae YNL271c BNI1 regulator of
budding - Yarrowia lipolytica (Candida lipolytica)
Length = 1851
Score = 37.1 bits (82), Expect = 0.48
Identities = 26/82 (31%), Positives = 26/82 (31%), Gaps = 2/82 (2%)
Frame = +2
Query: 422 PXXXXXPPXXGXGGXXXXGGGGPPPPXXXXXXXFXXKKKXPFF--XPXKKXGXGAXPPPP 595
P PP GG GGPPPP F P F P G PPPP
Sbjct: 1045 PPPPPPPPPMFTGGPPPMFTGGPPPPPPPPPPGFTGGPPPPGFTGGPPPPGFTGGPPPPP 1104
Query: 596 PXXXXXXXXXXXXXXXGGXPPP 661
P GG PPP
Sbjct: 1105 P------PPPLPPGFTGGPPPP 1120
>UniRef50_UPI0000E4896A Cluster: PREDICTED: similar to CG33556-PA;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
similar to CG33556-PA - Strongylocentrotus purpuratus
Length = 1472
Score = 36.7 bits (81), Expect = 0.63
Identities = 22/74 (29%), Positives = 22/74 (29%)
Frame = +2
Query: 440 PPXXGXGGXXXXGGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXX 619
PP G G PPPP P P G G PPPPP
Sbjct: 420 PPPPPPPPPLPPGVGAPPPPPPPPPPPLPGGSCIP--PPPPPPGMGGAPPPPPPPPFPGG 477
Query: 620 XXXXXXXXGGXPPP 661
GG PPP
Sbjct: 478 VPPPPPLPGGAPPP 491
Score = 34.3 bits (75), Expect = 3.4
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = +1
Query: 574 GGAXPPPPPXXXXXXXXXXXXXXGGGPPP 660
GGA PPPPP GGGPPP
Sbjct: 486 GGAPPPPPPPPFPGGGVPPPPFPGGGPPP 514
>UniRef50_Q54RW5 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 952
Score = 35.1 bits (77), Expect = 1.9
Identities = 19/68 (27%), Positives = 24/68 (35%)
Frame = +2
Query: 458 GGXXXXGGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXXXXXXXX 637
G G GGPPPP + + + + G G+ PPPP
Sbjct: 364 GASMRGGRGGPPPPIGMQPEFYSPPMRGGYMG---RGGRGSPFPPPPYGATMSSGRGGYM 420
Query: 638 XXGGXPPP 661
GG PPP
Sbjct: 421 GRGGAPPP 428
>UniRef50_Q6CDQ5 Cluster: Similarity; n=2; Saccharomycetales|Rep:
Similarity - Yarrowia lipolytica (Candida lipolytica)
Length = 664
Score = 35.1 bits (77), Expect = 1.9
Identities = 18/53 (33%), Positives = 18/53 (33%)
Frame = +2
Query: 440 PPXXGXGGXXXXGGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPP 598
PP G G G PPPP F P P G PPPPP
Sbjct: 510 PPPPGPGPAAAGGPPPPPPPPPGAAPSFGSAAPPPPPGPPPAMSGGPPPPPPP 562
>UniRef50_P14734 Cluster: Protein fork head; n=8; Endopterygota|Rep:
Protein fork head - Drosophila melanogaster (Fruit fly)
Length = 510
Score = 35.1 bits (77), Expect = 1.9
Identities = 13/20 (65%), Positives = 13/20 (65%)
Frame = +2
Query: 440 PPXXGXGGXXXXGGGGPPPP 499
PP G GG GGGGPPPP
Sbjct: 25 PPSGGGGGGGGGGGGGPPPP 44
>UniRef50_UPI0000498915 Cluster: hypothetical protein 9.t00033; n=1;
Entamoeba histolytica HM-1:IMSS|Rep: hypothetical
protein 9.t00033 - Entamoeba histolytica HM-1:IMSS
Length = 540
Score = 33.9 bits (74), Expect = 4.5
Identities = 20/62 (32%), Positives = 21/62 (33%)
Frame = +2
Query: 476 GGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXXXXXXXXXXGGXP 655
G G PPPP P P + G GA PPPPP G P
Sbjct: 394 GCGAPPPPPPPAFDTGCGIPPPP--PPARGTGCGAPPPPPPLNAPPPPPPPAHGTGCGVP 451
Query: 656 PP 661
PP
Sbjct: 452 PP 453
>UniRef50_Q1ZXK2 Cluster: Actin-binding protein; n=2; Dictyostelium
discoideum|Rep: Actin-binding protein - Dictyostelium
discoideum AX4
Length = 1074
Score = 33.9 bits (74), Expect = 4.5
Identities = 19/53 (35%), Positives = 19/53 (35%), Gaps = 1/53 (1%)
Frame = +2
Query: 443 PXXGXGGXXXXGGGGPPP-PXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPP 598
P G GGG PPP P P P G GA PPPPP
Sbjct: 554 PDISNDGQPISGGGAPPPSPSPPPPISGGGAPPPPPPPPPPPSGGGAPPPPPP 606
>UniRef50_Q5C3I4 Cluster: SJCHGC03138 protein; n=2; Schistosoma
japonicum|Rep: SJCHGC03138 protein - Schistosoma
japonicum (Blood fluke)
Length = 156
Score = 33.5 bits (73), Expect = 5.9
Identities = 27/77 (35%), Positives = 27/77 (35%), Gaps = 2/77 (2%)
Frame = -3
Query: 485 PPXXXXXPPPXXXXGGXXXXXVFXXKXXGGFF*KXGAXPXXXGXPGXFXFFF--F*PRGK 312
PP PPP GG F GG G P G G F FFF F P GK
Sbjct: 48 PPPPPGGPPPPFGGGGFPPFFHFFPPPGGGVAPPQGGGPPLGG--GGFQFFFKGFLP-GK 104
Query: 311 XGAXPTFFXXGGXFFXG 261
FF G F G
Sbjct: 105 IFLGSFFFFGGNPPFFG 121
>UniRef50_Q7JP75 Cluster: Cytokinesis defect protein 1, isoform b;
n=6; cellular organisms|Rep: Cytokinesis defect protein
1, isoform b - Caenorhabditis elegans
Length = 1437
Score = 33.1 bits (72), Expect = 7.8
Identities = 19/59 (32%), Positives = 19/59 (32%)
Frame = +2
Query: 422 PXXXXXPPXXGXGGXXXXGGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPP 598
P PP GG GG PPPP P P G PPPPP
Sbjct: 721 PITGGPPPPPPPGGLPPITGGPPPPPPPGGLPPISGGPPPP---PPPPGGCPPPPPPPP 776
>UniRef50_A5JUU8 Cluster: Formin B; n=2; Trypanosoma brucei|Rep:
Formin B - Trypanosoma brucei TREU927
Length = 1004
Score = 33.1 bits (72), Expect = 7.8
Identities = 23/77 (29%), Positives = 23/77 (29%)
Frame = +2
Query: 440 PPXXGXGGXXXXGGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXX 619
PP G GG PPPP P P K G G PPPPP
Sbjct: 514 PPPPGKAPPPPPGGKLPPPPPPGGKGA-----PPPPPPPPGKLGPGGGPPPPPPPPPRGL 568
Query: 620 XXXXXXXXGGXPPPXXK 670
GG P K
Sbjct: 569 GSLGVSALGGPKAPVPK 585
>UniRef50_A2DM28 Cluster: Diaphanous, putative; n=1; Trichomonas
vaginalis G3|Rep: Diaphanous, putative - Trichomonas
vaginalis G3
Length = 620
Score = 33.1 bits (72), Expect = 7.8
Identities = 18/58 (31%), Positives = 18/58 (31%)
Frame = +2
Query: 488 PPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXXXXXXXXXXGGXPPP 661
PPPP P G GA PPPPP GG PPP
Sbjct: 502 PPPPSAPGVPPAPPLPGAGVPPPPPPPGAGAPPPPPPPAGGAPPPPPPPPPKGGAPPP 559
>UniRef50_A0CZ14 Cluster: Chromosome undetermined scaffold_31, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_31,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 417
Score = 33.1 bits (72), Expect = 7.8
Identities = 17/58 (29%), Positives = 19/58 (32%)
Frame = +2
Query: 488 PPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPPXXXXXXXXXXXXXXXGGXPPP 661
PPPP ++ P P G A PPPPP G PPP
Sbjct: 327 PPPPPPPPPPPIPGQQNPPPPPPPPLPGQQAPPPPPPLPGGARPPPPPPPPFGNAPPP 384
>UniRef50_Q1E467 Cluster: Putative uncharacterized protein; n=1;
Coccidioides immitis|Rep: Putative uncharacterized
protein - Coccidioides immitis
Length = 1705
Score = 33.1 bits (72), Expect = 7.8
Identities = 16/41 (39%), Positives = 16/41 (39%)
Frame = +2
Query: 476 GGGGPPPPXXXXXXXFXXKKKXPFFXPXKKXGXGAXPPPPP 598
G GPPPP F P P GA PPPPP
Sbjct: 990 GFSGPPPPPPPPLPGFSGGPPPPPPPPLPGFSGGAPPPPPP 1030
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 496,217,865
Number of Sequences: 1657284
Number of extensions: 9460132
Number of successful extensions: 21817
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 8356
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18520
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 64204279620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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