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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_L20
         (882 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g57290.1 68416.m06377 eukaryotic translation initiation facto...   161   6e-40
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)...    31   1.0  
At3g61140.1 68416.m06842 COP9 signalosome complex subunit 1 / CS...    28   9.5  

>At3g57290.1 68416.m06377 eukaryotic translation initiation factor
           3E / eIF3e (TIF3E1) identical to eukaryotic initiation
           factor 3E subunit [Arabidopsis thaliana]
           gi|12407658|gb|AAG53613
          Length = 441

 Score =  161 bits (391), Expect = 6e-40
 Identities = 77/201 (38%), Positives = 128/201 (63%)
 Frame = +3

Query: 171 SYSKFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKXNMIDYVTDIRR 350
           S   +DLT  I   LDRHLVFP+ EFL  ++ Y   ++L++K+++L++ NM+DY  DI +
Sbjct: 4   SKQNYDLTPLIAPNLDRHLVFPIFEFLQERQLYPDEQILKSKIQLLNQTNMVDYAMDIHK 63

Query: 351 MLYPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDDVMKTIETMRDPKTLINHLS 530
            LY  ED P+E+++RR  V++ L+ L++A  P++  +   +    ++ +R  K   N   
Sbjct: 64  SLYHTEDAPQEMVERRTEVVARLKSLEEAAAPLVSFLLNPN---AVQELRADKQ-YNLQM 119

Query: 531 TNKEYEFKIEMIDSMYRLAKYRYECGNYVESASYLYFCQLVMSPTDKNYLSVLWGKLAXE 710
             + Y+   + I+++Y+ AK+++ECGNY  +A YLY  + + S  +++ LS LWGKLA E
Sbjct: 120 LKERYQIGPDQIEALYQYAKFQFECGNYSGAADYLYQYRTLCSNLERS-LSALWGKLASE 178

Query: 711 ILXXNWDGALDDLTKXREFID 773
           IL  NWD AL++L + +E ID
Sbjct: 179 ILMQNWDIALEELNRLKEIID 199


>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
           Similar to Arabidopsis luminal binding protein
           (gb|D89342); contains Pfam domain PF00012: dnaK protein
          Length = 678

 Score = 31.1 bits (67), Expect = 1.0
 Identities = 28/115 (24%), Positives = 54/115 (46%), Gaps = 5/115 (4%)
 Frame = +3

Query: 255 AKETYDQSELLQAKLEILSKX-----NMIDYVTDIRRMLYPEEDTPEEIIQRRGVVLSEL 419
           A+E  ++ ++++ K++  +K      NM   V D  ++     D  +E ++  GV+   L
Sbjct: 567 AEEFAEEDKIMKEKIDARNKLETYVYNMKSTVADKEKLAKKISDEDKEKME--GVLKEAL 624

Query: 420 QELQDAVEPVLRLMQRDDVMKTIETMRDPKTLINHLSTNKEYEFKIEMIDSMYRL 584
           + L++ V         D+ +K +E + DP     +  T  E E  +EMI   YR+
Sbjct: 625 EWLEENVNAEKE--DYDEKLKEVELVCDPVIKSVYEKTEGENEDTMEMITMSYRI 677


>At3g61140.1 68416.m06842 COP9 signalosome complex subunit 1 / CSN
           complex subunit 1 (CSN1) / COP11 protein (COP11) / FUSCA
           protein (FUS6) FUSCA6, COP11, CSN1; identical to FUS6
           GI:432446, SP:P45432 from [Arabidopsis thaliana];
           contains Pfam profile PF01399: PCI domain; identical to
           cDNA CSN complex subunit 1 (CSN1) GI:18056652
          Length = 441

 Score = 27.9 bits (59), Expect = 9.5
 Identities = 11/36 (30%), Positives = 23/36 (63%)
 Frame = +3

Query: 249 LAAKETYDQSELLQAKLEILSKXNMIDYVTDIRRML 356
           L A  ++D+SEL Q  ++ ++  N ++ V D+R ++
Sbjct: 264 LCALASFDRSELKQKVIDNINFRNFLELVPDVRELI 299


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,575,499
Number of Sequences: 28952
Number of extensions: 273177
Number of successful extensions: 672
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 670
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2077687200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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