BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_L18 (877 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g41530.1 68415.m05132 esterase, putative similar to SP|P10768... 153 2e-37 At1g03890.1 68414.m00373 cupin family protein similar to Arabido... 30 1.8 At5g23575.1 68418.m02766 transmembrane protein, putative similar... 29 4.1 At1g68910.1 68414.m07886 expressed protein similar to Myosin hea... 29 4.1 At5g15070.1 68418.m01766 expressed protein 28 7.1 At2g32230.1 68415.m03938 pentatricopeptide (PPR) repeat-containi... 28 7.1 At1g48390.1 68414.m05405 syntaxin-related family protein contain... 28 9.4 >At2g41530.1 68415.m05132 esterase, putative similar to SP|P10768 Esterase D (EC 3.1.1.1) {Homo sapiens}; contains Pfam profile: PF00756 putative esterase Length = 284 Score = 153 bits (370), Expect = 2e-37 Identities = 86/225 (38%), Positives = 114/225 (50%) Frame = +3 Query: 183 SNKIFGGYQKVYSHASSELKCKMNFSIYLPPQAEGGDVKLPLLYYLSGLTCSEQNFITKS 362 S K+F GY K Y H S L C M FSIY PP A K P+LY+LSGLTC+++NFI KS Sbjct: 10 STKMFDGYNKRYKHFSETLGCSMTFSIYFPPSASSSH-KSPVLYWLSGLTCTDENFIIKS 68 Query: 363 GFQRYAAEHGVIVVGPDTSPRGVKIDGDDSSWDFGVSAGFYLDATNEPWNNNYRMGSYLN 542 G QR A+ HG+ +V PDTSPRG+ ++G+ S+DFGV AGFYL+AT E W N+RM Y+ Sbjct: 69 GAQRAASTHGIALVAPDTSPRGLNVEGEADSYDFGVGAGFYLNATQEKW-KNWRMYDYVV 127 Query: 543 VELYDLILKAFCNVVDPNRXXXXXXXXXXXXCFXFYSEESWPVQVSKRFCCYL*SKCLSM 722 EL L+ + F + +D + Y + F + Sbjct: 128 KELPKLLSENF-SQLDTTKASISGHSMGGHGALTIYLRNLDKYKSVSAFAPITNPINCAW 186 Query: 723 GSEGFXWIFXXXXXXXXXXXCHRAGXKYNGPPLTLLLDQGSGDKF 857 G + F KYN T+L+DQG D+F Sbjct: 187 GQKAFTNYLGDNKAAWEEYDATCLISKYNNLSATILIDQGENDQF 231 Score = 72.9 bits (171), Expect = 3e-13 Identities = 34/57 (59%), Positives = 40/57 (70%) Frame = +1 Query: 628 GMGALXSTLRNPGQYKSVSAFAAICNPSACPWGVKAFXGYLGEDKSKWAEWDATELV 798 G GAL LRN +YKSVSAFA I NP C WG KAF YLG++K+ W E+DAT L+ Sbjct: 155 GHGALTIYLRNLDKYKSVSAFAPITNPINCAWGQKAFTNYLGDNKAAWEEYDATCLI 211 >At1g03890.1 68414.m00373 cupin family protein similar to Arabidopsis thaliana 12S seed storage proteins SP|P15455 [gi|808937] and SP|P15456, Brassica napus cruciferin storage protein, gi|762919, and others; contains Pfam profile PF00190 Cupin; Location of ESTs YAY049-3' end, gb|Z26364 and YAY049-5' end, gb|Z26363 Length = 451 Score = 30.3 bits (65), Expect = 1.8 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +3 Query: 132 FPEAHQKSNMDSLQLESSNKIFGGYQKVYSHASSELKC 245 FP A S ++SL + K G +V+ H S EL+C Sbjct: 32 FPNACHFSQINSLAPAQATKFEAGQMEVWDHMSPELRC 69 >At5g23575.1 68418.m02766 transmembrane protein, putative similar to cleft lip and palate transmembrane protein 1 [Homo sapiens] GI:4039014; contains Pfam profile PF05602: Cleft lip and palate transmembrane protein 1 (CLPTM1) Length = 593 Score = 29.1 bits (62), Expect = 4.1 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = -1 Query: 469 TPKSHDESSPSILTPRGEVSGPTTITPCSAAYLWKPDLVM 350 +PK DES P + + S P P LWKP++ + Sbjct: 182 SPKDSDESEPEVEKVGDKKSDPKEEVPVEWISLWKPNVTI 221 >At1g68910.1 68414.m07886 expressed protein similar to Myosin heavy chain, nonmuscle type B (Cellular myosin heavy chain, type B) (Nonmuscle myosin heavy chain-B) (NMMHC-B) (Swiss-Prot:Q27991) [Bos taurus]; contains 1 transmembrane domain Length = 627 Score = 29.1 bits (62), Expect = 4.1 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = +1 Query: 589 ILIELVLWDTAWEGMGALXSTLRNPGQYKSVSAFAAIC 702 +L+ L+ WD EGMG L S +P ++ F +C Sbjct: 47 LLMHLLAWDNDLEGMGTLDS---SPASFEKALTFDLLC 81 >At5g15070.1 68418.m01766 expressed protein Length = 1049 Score = 28.3 bits (60), Expect = 7.1 Identities = 24/86 (27%), Positives = 36/86 (41%) Frame = +3 Query: 153 SNMDSLQLESSNKIFGGYQKVYSHASSELKCKMNFSIYLPPQAEGGDVKLPLLYYLSGLT 332 S M++ + + + I G + V+ H SSEL M LPP A D LP L L+ Sbjct: 590 SEMEAAKAQLNEIITAGSKMVHDHVSSELPW-MTDGAGLPPHA---DEHLPELVKLAKKV 645 Query: 333 CSEQNFITKSGFQRYAAEHGVIVVGP 410 + + + + A VV P Sbjct: 646 TEQVRLLAQDEHENLAEPSAYDVVPP 671 >At2g32230.1 68415.m03938 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 572 Score = 28.3 bits (60), Expect = 7.1 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Frame = -2 Query: 204 NHQKFYWTILIASCPCLI--SDEPPEKLYSQLXCNHYLPR 91 ++ +YW SC CL+ +DE + L+ QL N + PR Sbjct: 472 SNDDWYWLYAAVSCKCLLVTNDEMRDHLF-QLLGNSFFPR 510 >At1g48390.1 68414.m05405 syntaxin-related family protein contains a novel domain similar to F-box that is shared among other proteins in Arabidopsis; similar to proteins At3g54160, At1g47920 (syntaxin SYP81), At5g41830, At3g44180, At3g58890, At1g56610, At1g48390, At3g59270 [Arabidopsis thaliana] Length = 413 Score = 27.9 bits (59), Expect = 9.4 Identities = 11/21 (52%), Positives = 12/21 (57%) Frame = +3 Query: 438 DGDDSSWDFGVSAGFYLDATN 500 DGD WDF V +Y D TN Sbjct: 235 DGDVYGWDFNVPFDYYGDVTN 255 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,518,260 Number of Sequences: 28952 Number of extensions: 416722 Number of successful extensions: 868 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 843 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 867 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 2058178400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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