Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_L02
(847 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast... 155 3e-38
At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic... 152 2e-37
At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic... 136 2e-32
At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic... 135 4e-32
At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast... 115 3e-26
At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast... 115 3e-26
At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondri... 112 2e-25
At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase... 28 9.0
At3g46360.1 68416.m05021 hypothetical protein 28 9.0
>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
transaminase A (ASP3) (YLS4) identical to SP|P46644
Aspartate aminotransferase, chloroplast precursor (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
identical to cDNA YLS4 mRNA for aspartate
aminotransferase (ASP3), partial cds GI:13122285
Length = 449
Score = 155 bits (377), Expect = 3e-38
Identities = 78/182 (42%), Positives = 103/182 (56%)
Frame = +1
Query: 199 VNLGVGAXSAXNGKPWVLPIVRKLEKQLAAXETLLHEYLPVLGLEQFCNASVAMLLGEDS 378
+NLGVGA GKP VL +VRK E+QL T + EYLP++GL +F S ++LG DS
Sbjct: 76 LNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRTRIKEYLPIVGLVEFNKLSAKLILGADS 135
Query: 379 PAIAAGKAFGVQVLSGTGGLRVGAEFLNKHLKYDTFYYSTPTWENHHLVFVNSGFTKPRL 558
PAI + V+ LSGTG LRVG EFL KH T Y + PTW NH +F +G T +
Sbjct: 136 PAIRENRITTVECLSGTGSLRVGGEFLAKHYHQKTIYITQPTWGNHPKIFTLAGLT-VKT 194
Query: 559 VPXLGREDQGHXLRXFHRRXQERSENAVILLHACAHNPTGIXPTXEQWVXIXDVMEERXL 738
+G + + +++LLHACAHNPTG+ PT +QW I +M + L
Sbjct: 195 YRYYDPATRGLNFQGLLEDLGAAAPGSIVLLHACAHNPTGVDPTIQQWEQIRKLMRSKGL 254
Query: 739 XP 744
P
Sbjct: 255 MP 256
Score = 45.6 bits (103), Expect = 4e-05
Identities = 32/88 (36%), Positives = 40/88 (45%), Gaps = 3/88 (3%)
Frame = +2
Query: 533 TLVSRSPXSYRYWDAKTRGIXFXGFIEXLKSARR---MPSYCCTHXXXXXXXXXXXXNSG 703
TL + +YRY+D TRG+ F G +E L +A + + C H
Sbjct: 186 TLAGLTVKTYRYYDPATRGLNFQGLLEDLGAAAPGSIVLLHACAHNPTGVDPTIQQWEQI 245
Query: 704 *XXLMSWRSASCXRFFDXAYQGFASGDL 787
LM RS FFD AYQGFASG L
Sbjct: 246 -RKLM--RSKGLMPFFDSAYQGFASGSL 270
>At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic
isozyme 1 / transaminase A (ASP2) identical to SP|P46645
Aspartate aminotransferase, cytoplasmic isozyme 1 (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana}
Length = 405
Score = 152 bits (369), Expect = 2e-37
Identities = 77/182 (42%), Positives = 98/182 (53%)
Frame = +1
Query: 199 VNLGVGAXSAXNGKPWVLPIVRKLEKQLAAXETLLHEYLPVLGLEQFCNASVAMLLGEDS 378
+NLGVGA GKP VL +VRK E+QL + + EY+P++G+ F S ++LG DS
Sbjct: 32 INLGVGAYRTEEGKPLVLDVVRKAEQQLVNDPSRVKEYIPIVGISDFNKLSAKLILGADS 91
Query: 379 PAIAAGKAFGVQVLSGTGGLRVGAEFLNKHLKYDTFYYSTPTWENHHLVFVNSGFTKPRL 558
PAI + VQ LSGTG LRVGAEFL H Y PTW NH VF +G +
Sbjct: 92 PAITESRVTTVQCLSGTGSLRVGAEFLKTHYHQSVIYIPKPTWGNHPKVFNLAGLS-VEY 150
Query: 559 VPXLGREDQGHXLRXFHRRXQERSENAVILLHACAHNPTGIXPTXEQWVXIXDVMEERXL 738
+G + A++LLHACAHNPTG+ PT EQW I +M + L
Sbjct: 151 FRYYDPATRGLDFKGLLEDLGAAPSGAIVLLHACAHNPTGVDPTSEQWEQIRQLMRSKSL 210
Query: 739 XP 744
P
Sbjct: 211 LP 212
Score = 43.6 bits (98), Expect = 2e-04
Identities = 32/87 (36%), Positives = 39/87 (44%), Gaps = 3/87 (3%)
Frame = +2
Query: 536 LVSRSPXSYRYWDAKTRGIXFXGFIEXLKSARR---MPSYCCTHXXXXXXXXXXXXNSG* 706
L S +RY+D TRG+ F G +E L +A + + C H
Sbjct: 143 LAGLSVEYFRYYDPATRGLDFKGLLEDLGAAPSGAIVLLHACAHNPTGVDPTSEQWEQI- 201
Query: 707 XXLMSWRSASCXRFFDXAYQGFASGDL 787
LM RS S FFD AYQGFASG L
Sbjct: 202 RQLM--RSKSLLPFFDSAYQGFASGSL 226
>At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 405
Score = 136 bits (329), Expect = 2e-32
Identities = 70/182 (38%), Positives = 97/182 (53%)
Frame = +1
Query: 199 VNLGVGAXSAXNGKPWVLPIVRKLEKQLAAXETLLHEYLPVLGLEQFCNASVAMLLGEDS 378
+NL G GKP VL +VR+ E+QLA + EYLP+ GL +F S ++LG+DS
Sbjct: 32 LNLSAGTYRTEEGKPLVLDVVRRAEQQLANDLSRDKEYLPLNGLPEFNKLSTKLILGDDS 91
Query: 379 PAIAAGKAFGVQVLSGTGGLRVGAEFLNKHLKYDTFYYSTPTWENHHLVFVNSGFTKPRL 558
PA+ + Q LSGTG LRVGAEFL H K + PTW NH +F +G + +
Sbjct: 92 PALKENRVVTTQCLSGTGSLRVGAEFLATHNKESVIFVPNPTWGNHPRIFTLAGLS-VQY 150
Query: 559 VPXLGREDQGHXLRXFHRRXQERSENAVILLHACAHNPTGIXPTXEQWVXIXDVMEERXL 738
+ +G + A+++L ACAHNPTG+ PT EQW I ++ + L
Sbjct: 151 FRYYDPKSRGLDFKGMLEDLGAAPPGAIVVLQACAHNPTGVDPTFEQWEKIRRLVRSKSL 210
Query: 739 XP 744
P
Sbjct: 211 LP 212
Score = 44.4 bits (100), Expect = 1e-04
Identities = 33/98 (33%), Positives = 41/98 (41%), Gaps = 3/98 (3%)
Frame = +2
Query: 533 TLVSRSPXSYRYWDAKTRGIXFXGFIEXLKSARR---MPSYCCTHXXXXXXXXXXXXNSG 703
TL S +RY+D K+RG+ F G +E L +A + C H
Sbjct: 142 TLAGLSVQYFRYYDPKSRGLDFKGMLEDLGAAPPGAIVVLQACAHNPTGVDPTFEQWEKI 201
Query: 704 *XXLMSWRSASCXRFFDXAYQGFASGDLXXXPWAXXXF 817
+ RS S FFD AYQGFASG L A F
Sbjct: 202 RRLV---RSKSLLPFFDSAYQGFASGSLDADAQAVRMF 236
>At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 403
Score = 135 bits (326), Expect = 4e-32
Identities = 71/182 (39%), Positives = 97/182 (53%)
Frame = +1
Query: 199 VNLGVGAXSAXNGKPWVLPIVRKLEKQLAAXETLLHEYLPVLGLEQFCNASVAMLLGEDS 378
+NL G GKP VL +VR+ E+QLA L EYLP+ GL +F S ++LG+DS
Sbjct: 32 LNLSAGTYRTEEGKPLVLDVVRRAEQQLA--NDLDKEYLPLNGLPEFNKLSTKLILGDDS 89
Query: 379 PAIAAGKAFGVQVLSGTGGLRVGAEFLNKHLKYDTFYYSTPTWENHHLVFVNSGFTKPRL 558
PA+ + Q LSGTG LRVGAEFL H K + PTW NH +F +G + +
Sbjct: 90 PALKENRVVTTQCLSGTGSLRVGAEFLATHNKESVIFVPNPTWGNHPRIFTLAGLS-VQY 148
Query: 559 VPXLGREDQGHXLRXFHRRXQERSENAVILLHACAHNPTGIXPTXEQWVXIXDVMEERXL 738
+ +G + A+++L ACAHNPTG+ PT EQW I ++ + L
Sbjct: 149 FRYYDPKSRGLDFKGMLEDLGAAPPGAIVVLQACAHNPTGVDPTFEQWEKIRRLVRSKSL 208
Query: 739 XP 744
P
Sbjct: 209 LP 210
Score = 44.4 bits (100), Expect = 1e-04
Identities = 33/98 (33%), Positives = 41/98 (41%), Gaps = 3/98 (3%)
Frame = +2
Query: 533 TLVSRSPXSYRYWDAKTRGIXFXGFIEXLKSARR---MPSYCCTHXXXXXXXXXXXXNSG 703
TL S +RY+D K+RG+ F G +E L +A + C H
Sbjct: 140 TLAGLSVQYFRYYDPKSRGLDFKGMLEDLGAAPPGAIVVLQACAHNPTGVDPTFEQWEKI 199
Query: 704 *XXLMSWRSASCXRFFDXAYQGFASGDLXXXPWAXXXF 817
+ RS S FFD AYQGFASG L A F
Sbjct: 200 RRLV---RSKSLLPFFDSAYQGFASGSLDADAQAVRMF 234
>At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 115 bits (277), Expect = 3e-26
Identities = 68/182 (37%), Positives = 90/182 (49%)
Frame = +1
Query: 199 VNLGVGAXSAXNGKPWVLPIVRKLEKQLAAXETLLHEYLPVLGLEQFCNASVAMLLGEDS 378
+NLGVGA +P+VL +V+K E L EYLP+ GL F A+ +L G
Sbjct: 80 LNLGVGAYRTEELQPYVLNVVKKAEN-LMLERGDNKEYLPIEGLAAFNKATAELLFGAGH 138
Query: 379 PAIAAGKAFGVQVLSGTGGLRVGAEFLNKHLKYDTFYYSTPTWENHHLVFVNSGFTKPRL 558
P I + +Q LSGTG LR+ A + ++ S+PTW NH +F N
Sbjct: 139 PVIKEQRVATIQGLSGTGSLRLAAALIERYFPGAKVVISSPTWGNHKNIF-NDAKVPWSE 197
Query: 559 VPXLGREDQGHXLRXFHRRXQERSENAVILLHACAHNPTGIXPTXEQWVXIXDVMEERXL 738
+ G +E E + ILLH CAHNPTGI PT EQWV I DV++E+
Sbjct: 198 YRYYDPKTIGLDFEGMIADIKEAPEGSFILLHGCAHNPTGIDPTPEQWVKIADVIQEKNH 257
Query: 739 XP 744
P
Sbjct: 258 IP 259
Score = 37.9 bits (84), Expect = 0.008
Identities = 26/77 (33%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
Frame = +2
Query: 560 YRYWDAKTRGIXFXGFIEXLKSARRMPSYCCTHXXXXXXXXXXXXNSG*XXLMSW-RSAS 736
YRY+D KT G+ F G I +K A S+ H + + +
Sbjct: 198 YRYYDPKTIGLDFEGMIADIKEAPE-GSFILLHGCAHNPTGIDPTPEQWVKIADVIQEKN 256
Query: 737 CXRFFDXAYQGFASGDL 787
FFD AYQGFASG L
Sbjct: 257 HIPFFDVAYQGFASGSL 273
>At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 115 bits (277), Expect = 3e-26
Identities = 68/182 (37%), Positives = 90/182 (49%)
Frame = +1
Query: 199 VNLGVGAXSAXNGKPWVLPIVRKLEKQLAAXETLLHEYLPVLGLEQFCNASVAMLLGEDS 378
+NLGVGA +P+VL +V+K E L EYLP+ GL F A+ +L G
Sbjct: 80 LNLGVGAYRTEELQPYVLNVVKKAEN-LMLERGDNKEYLPIEGLAAFNKATAELLFGAGH 138
Query: 379 PAIAAGKAFGVQVLSGTGGLRVGAEFLNKHLKYDTFYYSTPTWENHHLVFVNSGFTKPRL 558
P I + +Q LSGTG LR+ A + ++ S+PTW NH +F N
Sbjct: 139 PVIKEQRVATIQGLSGTGSLRLAAALIERYFPGAKVVISSPTWGNHKNIF-NDAKVPWSE 197
Query: 559 VPXLGREDQGHXLRXFHRRXQERSENAVILLHACAHNPTGIXPTXEQWVXIXDVMEERXL 738
+ G +E E + ILLH CAHNPTGI PT EQWV I DV++E+
Sbjct: 198 YRYYDPKTIGLDFEGMIADIKEAPEGSFILLHGCAHNPTGIDPTPEQWVKIADVIQEKNH 257
Query: 739 XP 744
P
Sbjct: 258 IP 259
Score = 37.9 bits (84), Expect = 0.008
Identities = 26/77 (33%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
Frame = +2
Query: 560 YRYWDAKTRGIXFXGFIEXLKSARRMPSYCCTHXXXXXXXXXXXXNSG*XXLMSW-RSAS 736
YRY+D KT G+ F G I +K A S+ H + + +
Sbjct: 198 YRYYDPKTIGLDFEGMIADIKEAPE-GSFILLHGCAHNPTGIDPTPEQWVKIADVIQEKN 256
Query: 737 CXRFFDXAYQGFASGDL 787
FFD AYQGFASG L
Sbjct: 257 HIPFFDVAYQGFASGSL 273
>At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondrial
/ transaminase A (ASP1) identical to SP|P46643 Aspartate
aminotransferase, mitochondrial precursor (EC 2.6.1.1)
(Transaminase A) {Arabidopsis thaliana}
Length = 430
Score = 112 bits (270), Expect = 2e-25
Identities = 63/178 (35%), Positives = 92/178 (51%)
Frame = +1
Query: 199 VNLGVGAXSAXNGKPWVLPIVRKLEKQLAAXETLLHEYLPVLGLEQFCNASVAMLLGEDS 378
VN+GVGA NGKP VL VR+ EK+LA + EYLP+ G + + ++ + G++S
Sbjct: 60 VNVGVGAYRDDNGKPVVLECVREAEKRLAGSTFM--EYLPMGGSAKMVDLTLKLAYGDNS 117
Query: 379 PAIAAGKAFGVQVLSGTGGLRVGAEFLNKHLKYDTFYYSTPTWENHHLVFVNSGFTKPRL 558
I + VQ LSGTG R+ A+F + Y PTW NHH ++ ++ + +
Sbjct: 118 EFIKDKRIAAVQTLSGTGACRLFADFQKRFSPGSQIYIPVPTWSNHHNIWKDAQVPQ-KT 176
Query: 559 VPXLGREDQGHXLRXFHRRXQERSENAVILLHACAHNPTGIXPTXEQWVXIXDVMEER 732
E +G + E + LLHACAHNPTG+ PT EQW I + + +
Sbjct: 177 YHYYHPETKGLDFSALMDDVKNAPEGSFFLLHACAHNPTGVDPTEEQWREISQLFKAK 234
Score = 35.1 bits (77), Expect = 0.059
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 1/77 (1%)
Frame = +2
Query: 557 SYRYWDAKTRGIXFXGFIEXLKSARRMPSYCCTHXXXXXXXXXXXXNSG*XXLMS-WRSA 733
+Y Y+ +T+G+ F ++ +K+A S+ H + +++
Sbjct: 176 TYHYYHPETKGLDFSALMDDVKNAPE-GSFFLLHACAHNPTGVDPTEEQWREISQLFKAK 234
Query: 734 SCXRFFDXAYQGFASGD 784
FFD AYQGFASGD
Sbjct: 235 KHFAFFDMAYQGFASGD 251
>At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase,
putative / 1-deoxyxylulose-5-phosphate synthase,
putative / DXP-synthase, putative (DEF) (CLA1) identical
to SP|Q38854 Probable 1-deoxy-D-xylulose 5-phosphate
synthase, chloroplast precursor (EC 4.1.3.37)
(1-deoxyxylulose-5-phosphate synthase) (DXP synthase)
(DXPS). [Mouse-ear cress] {Arabidopsis thaliana}, DEF
(deficient in photosynthesis) protein [Arabidopsis
thaliana] GI:1399261
Length = 717
Score = 27.9 bits (59), Expect = 9.0
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -3
Query: 377 LSSPRSIATDALQNCSRPNTGRYSCSKVSXAAS 279
LSS RS+ TD C +PN +S + AS
Sbjct: 28 LSSSRSLVTDLPSPCLKPNNNSHSNRRAKVCAS 60
>At3g46360.1 68416.m05021 hypothetical protein
Length = 197
Score = 27.9 bits (59), Expect = 9.0
Identities = 14/44 (31%), Positives = 20/44 (45%)
Frame = -2
Query: 663 GACVQQYDGILRALLXSSMKPSKXMPLVFASQXRYEXGLRETRV 532
G CV + G+LR + S+M + P VF LR + V
Sbjct: 100 GQCVSSFGGLLRVVASSAMVMASPCPFVFVPDASSPVVLRSSFV 143
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,899,887
Number of Sequences: 28952
Number of extensions: 253951
Number of successful extensions: 735
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 689
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 716
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1960634400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -