Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_J17
(901 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 31 1.0
At5g09400.1 68418.m01089 potassium transporter family protein si... 31 1.4
At3g10960.1 68416.m01321 xanthine/uracil permease family protein... 30 1.8
At1g62250.2 68414.m07023 expressed protein 30 1.8
At1g62250.1 68414.m07022 expressed protein 30 1.8
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 29 5.6
At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 29 5.6
At1g64150.1 68414.m07267 expressed protein contains Pfam profile... 29 5.6
At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 28 9.7
At3g17550.1 68416.m02241 NLI interacting factor (NIF) family pro... 28 9.7
At3g10810.1 68416.m01301 zinc finger (C3HC4-type RING finger) fa... 28 9.7
At3g04160.1 68416.m00440 expressed protein ; expression supporte... 28 9.7
At2g39200.1 68415.m04815 seven transmembrane MLO family protein ... 28 9.7
At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain... 28 9.7
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 31.1 bits (67), Expect = 1.0
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
Frame = +1
Query: 211 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 384
+G Y+ A+ S + G I++ K LIE+ + T+D +L T+ G + S
Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557
Query: 385 --PIQFRVIFTEQTVKLINKRDHHA 453
P+ F +F + L++ + +A
Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582
>At5g09400.1 68418.m01089 potassium transporter family protein
similar to K+ transporter HAK5 [Arabidopsis thaliana]
GI:7108597; contains Pfam profile PF02705: K+ potassium
transporter; KUP/HAK/KT Transporter family member,
PMID:11500563; Note: possible sequencing error causes a
frameshift in the 4th exon|15810448|gb|AY056263
Length = 858
Score = 30.7 bits (66), Expect = 1.4
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -3
Query: 293 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 114
F +S+ S F+ + ++A ++ MTT +SC S+ LG L+ HT+R
Sbjct: 411 FFSSVPGSAFWPVLFIANIAALIASRTMTTATFSC-IKQSTALGCFPRLKI-IHTSRKFM 468
Query: 113 NKVXLXLAQWLXL 75
++ + + W L
Sbjct: 469 GQIYIPVLNWFLL 481
>At3g10960.1 68416.m01321 xanthine/uracil permease family protein
contains Pfam profile: PF00860 permease family
Length = 579
Score = 30.3 bits (65), Expect = 1.8
Identities = 16/73 (21%), Positives = 33/73 (45%)
Frame = -1
Query: 811 CPKXSRXSLAAISSXKCHNTVVLSVVDEEHDVAFVHGGLKVPVVFEGVSGAITVDDTVIT 632
CP SR SLA + + +L+ D+ +HG ++ P + G+ G + + ++
Sbjct: 254 CPASSRISLAPVITSANGTVSLLAGGSVSGDIMCIHGRMESPTFWLGIVGFVIIAYCLVK 313
Query: 631 RTFRVIELQVLFV 593
+ ++FV
Sbjct: 314 NVKGAMIYGIVFV 326
>At1g62250.2 68414.m07023 expressed protein
Length = 223
Score = 30.3 bits (65), Expect = 1.8
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +2
Query: 107 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 235
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At1g62250.1 68414.m07022 expressed protein
Length = 267
Score = 30.3 bits (65), Expect = 1.8
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +2
Query: 107 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 235
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase
(ATHIM) identical to SP|P34881 DNA
(cytosine-5)-methyltransferase AthI (EC 2.1.1.37)
{Arabidopsis thaliana}
Length = 1534
Score = 28.7 bits (61), Expect = 5.6
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -1
Query: 355 PLSITGRRSPWCSSCRFRSDASR 287
PLS GR S +C+SC+ R D +
Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891
>At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19)
Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis
thaliana]; similar to cytochrome P450LXXIA1, Persea
americana, M32885
Length = 490
Score = 28.7 bits (61), Expect = 5.6
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -2
Query: 453 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 361
G +P + +D + G+DH + + RFD+FL
Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251
>At1g64150.1 68414.m07267 expressed protein contains Pfam profile
PF01169: Uncharacterized protein family UPF0016
Length = 370
Score = 28.7 bits (61), Expect = 5.6
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -1
Query: 673 GVSGAITVDDTVITRTFRVIELQVLFVLGGHDLEVNSV 560
G G +T+ V+ RTF ++ + F GG DL ++ +
Sbjct: 197 GALGIMTIISVVLGRTFHYVDEVLPFRFGGTDLPIDDI 234
>At3g62000.1 68416.m06963 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 278
Score = 27.9 bits (59), Expect = 9.7
Identities = 26/126 (20%), Positives = 56/126 (44%), Gaps = 2/126 (1%)
Frame = +1
Query: 178 VLAEQLYMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKD 357
V E+ S+ + + +A S + K+G + E++K +I+NG+ + DFA+
Sbjct: 147 VACERDSNSLEVAKRYYELAGVSHKVNVKQG-LAAESLKSMIQNGEGASYDFAF---VDA 202
Query: 358 GKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALK--LIDQQNHNKIAFGDSKDKTSKKVS 531
K + + YF + +++ + + N H + +++ I + K K+VS
Sbjct: 203 DKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSDPMVNDAKTISIRNFNKKLMDDKRVS 262
Query: 532 WKFTPV 549
P+
Sbjct: 263 ISMVPI 268
>At3g17550.1 68416.m02241 NLI interacting factor (NIF) family
protein contains Pfam profile PF03031: NLI interacting
factor
Length = 296
Score = 27.9 bits (59), Expect = 9.7
Identities = 12/52 (23%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
Frame = +2
Query: 536 SLPPCLENNRVYF--KIMSTEDKQYLKLDNTKGSSDDRIIYGDSTADTFKHH 685
SL ++ R+YF ++++ ++ Y+K + + + ++ D T+D + HH
Sbjct: 165 SLLKLIDPKRIYFGDRVITRDESPYVKTLDLVLAEERGVVIVDDTSDVWTHH 216
>At3g10810.1 68416.m01301 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 Zinc
finger, C3HC4 type (RING finger)
Length = 496
Score = 27.9 bits (59), Expect = 9.7
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 5/32 (15%)
Frame = +2
Query: 497 VTPKTKPA-----RKSPGSLPPCLENNRVYFK 577
V+P PA +++P + PPC NRV+FK
Sbjct: 365 VSPVASPAPHRSRKRAPSAPPPCNPGNRVHFK 396
>At3g04160.1 68416.m00440 expressed protein ; expression supported
by MPSS
Length = 712
Score = 27.9 bits (59), Expect = 9.7
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +3
Query: 285 SREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV 395
SR ++E G +P DK WKG + + +P+
Sbjct: 563 SRSPIGNDQKESDFGYSIPSTDKQWKGENRADIEYPI 599
>At2g39200.1 68415.m04815 seven transmembrane MLO family protein /
MLO-like protein 12 (MLO12) identical to SP|O80961
MLO-like protein 12 (AtMlo12) {Arabidopsis thaliana},
membrane protein Mlo12 [Arabidopsis thaliana]
gi|14091594|gb|AAK53805; similar to MLO protein
SWISS-PROT:P93766, NCBI_gi:1877221 [Hordeum
vulgare][Barley]
Length = 576
Score = 27.9 bits (59), Expect = 9.7
Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = +3
Query: 549 VWKTTEFTSRSC-PPRTNST*SSITRKVLVMTVSSTVIAPL 668
VW T EFT ++C +T IT VL+ + S + PL
Sbjct: 383 VWSTYEFTLKNCFHHKTEDIAIRITMGVLIQVLCSYITLPL 423
>At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein low similarity to SP|Q99614
Tetratricopeptide repeat protein 1 {Homo sapiens};
contains Pfam profiles PF00564: PB1 domain, PF00515: TPR
Domain
Length = 697
Score = 27.9 bits (59), Expect = 9.7
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +1
Query: 196 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 342
YM + +GEY AI +C+ L E K +KR N +DFA++
Sbjct: 100 YMQMGLGEYPNAINECNLAL-EASPRFSKALLKRARCYEALNKLDFAFR 147
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,590,463
Number of Sequences: 28952
Number of extensions: 360633
Number of successful extensions: 1217
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1217
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2120147664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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