Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_J03
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 189 4e-49
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 119 5e-28
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 113 3e-26
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 112 5e-26
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 95 1e-20
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 92 1e-19
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 87 3e-18
SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 80 3e-16
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 40 6e-04
SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 29 0.83
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.8
>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
Cct3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 528
Score = 189 bits (461), Expect = 4e-49
Identities = 98/185 (52%), Positives = 131/185 (70%)
Frame = +3
Query: 246 LGPQAMLKMLMDPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVIV 425
LGP+AMLKML+DP+G +++TNDG+AILR I V HPAAKSMIE+ARTQDEEVGDGTTSVI+
Sbjct: 39 LGPRAMLKMLLDPVGSVLLTNDGHAILREIEVAHPAAKSMIELARTQDEEVGDGTTSVII 98
Query: 426 LAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQDKISVPVDLNDRDKMKEVIRSC 605
LAGE+LA A P L + IHP V+IR ++QALEDA+ ++ D+I++PV+++D +M +IR+C
Sbjct: 99 LAGEILAAASPLLDRKIHPVVMIRSFKQALEDALSII-DEITLPVNVDDNAEMFRLIRTC 157
Query: 606 VGTKYIGRWADLXRRHCS*CG*YCYCE**WQN*G*H*KLCKSGXDPGGTVEESRVLSGVM 785
+GTK + RW+DL + + + PGG +E S VL GVM
Sbjct: 158 IGTKLVARWSDLMCHLALRAVRTVASTSNGRMEIDIKRYARVEKVPGGEIESSCVLDGVM 217
Query: 786 FNKDV 800
NKDV
Sbjct: 218 LNKDV 222
Score = 41.9 bits (94), Expect = 1e-04
Identities = 19/41 (46%), Positives = 27/41 (65%)
Frame = +1
Query: 640 LXVDIALDAVNTVTVNDNGRIEVDIKNYAKVEXIQVAQLKS 762
L +AL AV TV NGR+E+DIK YA+VE + +++S
Sbjct: 169 LMCHLALRAVRTVASTSNGRMEIDIKRYARVEKVPGGEIES 209
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/43 (41%), Positives = 22/43 (51%)
Frame = +2
Query: 161 NXKRXSGRKVXLENXSAGKPIADVIXTCLGTSGHVKNVNGPYG 289
N R G K + N A K +ADVI TCLG +K + P G
Sbjct: 11 NGNRQVGHKAQMSNIQAAKAVADVIRTCLGPRAMLKMLLDPVG 53
Score = 30.3 bits (65), Expect = 0.36
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +2
Query: 803 HPKMXRYIENPRIIL 847
HPKM R IENPRI+L
Sbjct: 224 HPKMRRRIENPRIVL 238
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 119 bits (287), Expect = 5e-28
Identities = 55/132 (41%), Positives = 86/132 (65%)
Frame = +3
Query: 243 ALGPQAMLKMLMDPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVI 422
+LGP + KML+D +G + +TNDG IL + V+HPA K ++E+A+ QD+EVGDGTTSV+
Sbjct: 40 SLGPVGLDKMLVDDIGDVTVTNDGATILSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVV 99
Query: 423 VLAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQDKISVPVDLNDRDKMKEVIRS 602
++A E+L A + IHPT II YR A+ +A+ + D +S VD ++ + V ++
Sbjct: 100 IIAAELLRRANELVKNKIHPTTIITGYRLAIREAVKFMTDVLSCSVDSLGKESLINVAKT 159
Query: 603 CVGTKYIGRWAD 638
+ +K IG +D
Sbjct: 160 SMSSKIIGNDSD 171
>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
Cct4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 527
Score = 113 bits (272), Expect = 3e-26
Identities = 50/133 (37%), Positives = 89/133 (66%)
Frame = +3
Query: 243 ALGPQAMLKMLMDPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVI 422
+LGP+ M KM+ G +++TNDG IL+ ++V HPAAK +++++ QD E GDGTTSV+
Sbjct: 38 SLGPKGMDKMIQTGKGEVILTNDGATILKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVV 97
Query: 423 VLAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQDKISVPVDLNDRDKMKEVIRS 602
+LAG MLA AE L + IHPTVI +++A + +++ ++ ++L+DR+ + +
Sbjct: 98 ILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFTVDCMKEN-ALAIELSDRESLLRAATT 156
Query: 603 CVGTKYIGRWADL 641
+ +K + ++++L
Sbjct: 157 SLNSKIVSQYSNL 169
>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
Cct7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 558
Score = 112 bits (270), Expect = 5e-26
Identities = 51/123 (41%), Positives = 85/123 (69%)
Frame = +3
Query: 246 LGPQAMLKMLMDPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVIV 425
LGP K+++D G +V++NDG I++ + + HPAAK++++IAR QD EVGDGTTSV+V
Sbjct: 44 LGPLGADKLMVDDRGEVVISNDGATIMKLLDIVHPAAKTLVDIARAQDAEVGDGTTSVVV 103
Query: 426 LAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQDKISVPVDLNDRDKMKEVIRSC 605
AGE+L A F+ + +IIR YR+A + A+ +++ I++ +DL+D K+++++ C
Sbjct: 104 FAGELLREARTFVEDGVSSHLIIRGYRKAAQLAVNKIKE-IAIHLDLSDEGKLRDLLTKC 162
Query: 606 VGT 614
T
Sbjct: 163 AST 165
>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
Cct5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 546
Score = 94.7 bits (225), Expect = 1e-20
Identities = 60/188 (31%), Positives = 107/188 (56%), Gaps = 2/188 (1%)
Frame = +3
Query: 243 ALGPQAMLKMLMDPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVI 422
+LGP+ + K+L+ P G I +TNDG IL + V+H AK +++++++QD+E+GDGTT V+
Sbjct: 53 SLGPRGLDKILISPDGEITVTNDGATILDQMEVEHQIAKLLVQLSKSQDDEIGDGTTGVV 112
Query: 423 VLAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQDKISVPVDLNDRD--KMKEVI 596
VLAG +L AE + + IHP I Y +A + A+ L D IS VD + + +
Sbjct: 113 VLAGALLEQAEALIDKGIHPIRIADGYEKACQVAVKHL-DAISDVVDFSPENTTNLFRSA 171
Query: 597 RSCVGTKYIGRWADLXRRHCS*CG*YCYCE**WQN*G*H*KLCKSGXDPGGTVEESRVLS 776
++ +G+K + + D + + + Q +L K GG+V++++++
Sbjct: 172 KTSLGSKVVSKAHDHFA-NIAVDAVLSVAD--LQRKDVDFELIKVDGKVGGSVDDTKLVK 228
Query: 777 GVMFNKDV 800
GV+ +KD+
Sbjct: 229 GVVVDKDM 236
>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
Cct2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 91.9 bits (218), Expect = 1e-19
Identities = 55/133 (41%), Positives = 79/133 (59%), Gaps = 5/133 (3%)
Frame = +3
Query: 246 LGPQAMLKMLM-DPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVI 422
LGP+ M K+L + G IV+TNDG IL+ I + + AAK ++ I++ QD+EVGDGTTSV
Sbjct: 40 LGPKGMDKILQSNSSGDIVVTNDGATILKSIALDNAAAKVLVNISKVQDDEVGDGTTSVC 99
Query: 423 VLAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQ----DKISVPVDLNDRDKMKE 590
V A E+L AE + IHP VII YR A + AI L+ D S P R ++
Sbjct: 100 VFAAELLRQAEIMVNAKIHPQVIIDGYRIATKTAIDALRASSIDNSSDPAKF--RSDLEN 157
Query: 591 VIRSCVGTKYIGR 629
+ R+ + +K + +
Sbjct: 158 IARTTLSSKILSQ 170
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 87.0 bits (206), Expect = 3e-18
Identities = 44/124 (35%), Positives = 71/124 (57%)
Frame = +3
Query: 246 LGPQAMLKMLMDPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVIV 425
LGP KML+D G I +T DG +L + +Q+P A + + A QD+ GDGTTSV +
Sbjct: 36 LGPTGTTKMLVDGAGAIKLTKDGKVLLTEMQIQNPTASCIAKAATAQDDATGDGTTSVCL 95
Query: 426 LAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQDKISVPVDLNDRDKMKEVIRSC 605
L GE+L AE ++ + +HP++I + A +A+ L D ++ DR+ + V ++
Sbjct: 96 LVGELLKQAELYIREGLHPSLISDGFNLAKNEALTFL-DSFKTDFEV-DREVLLNVAKTS 153
Query: 606 VGTK 617
+ TK
Sbjct: 154 LSTK 157
>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 546
Score = 80.2 bits (189), Expect = 3e-16
Identities = 39/129 (30%), Positives = 73/129 (56%), Gaps = 1/129 (0%)
Frame = +3
Query: 243 ALGPQAMLKMLMDPMGGIVMTNDGNAILRXITVQHPAAKSMIEIARTQDEEVGDGTTSVI 422
+LGP K++++ + +TND I+R + V HPAAK +++ + Q+ E+GD V+
Sbjct: 45 SLGPNGKNKIVVNHLQQTFLTNDAATIIRELEVIHPAAKLVVDATQQQENELGDAANFVV 104
Query: 423 VLAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQDKISVPVDLNDRDK-MKEVIR 599
V GE+LA AE + + P I + Y AL + +L++ + ++ + +K + + IR
Sbjct: 105 VFTGELLAKAENMIRMGLTPLEIAKGYEMALSHTMEVLEEICADKIETVESEKELIKAIR 164
Query: 600 SCVGTKYIG 626
+C+ +K G
Sbjct: 165 TCISSKQYG 173
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 39.5 bits (88), Expect = 6e-04
Identities = 24/102 (23%), Positives = 50/102 (49%), Gaps = 4/102 (3%)
Frame = +3
Query: 246 LGPQAMLKMLMDPMGGIVMTNDGNAILRXITV----QHPAAKSMIEIARTQDEEVGDGTT 413
LGP+ ++ P G +T DG + R +++ ++ A+ + ++A +E GDGTT
Sbjct: 62 LGPKGRNVLIDQPFGSPKITKDGVTVARSVSLKDKFENLGARLVQDVASKTNEVAGDGTT 121
Query: 414 SVIVLAGEMLAIAEPFLTQNIHPTVIIREYRQALEDAIVLLQ 539
+ VL + + + +P + R + A+++ + LQ
Sbjct: 122 TATVLTRAIFSETVRNVAAGCNPMDLRRGIQLAVDNVVEFLQ 163
>SPCC417.06c |ppk35|mug27|serine/threonine protein kinase
Ppk35|Schizosaccharomyces pombe|chr 3|||Manual
Length = 624
Score = 29.1 bits (62), Expect = 0.83
Identities = 22/87 (25%), Positives = 36/87 (41%), Gaps = 3/87 (3%)
Frame = +2
Query: 266 KNVNGPY---GRHRDDQRW*CYTQXNHSPTSSCKIYDRNRKDPR*RGWRWNHISHSASRR 436
KN NG Y R D+ W T+ PTS + +K P + WN + A
Sbjct: 421 KNENGIYDKKARSVSDEAWSFITKCLTEPTSRFQSTIEIQKHPFFKRLHWNGLRKRAVPP 480
Query: 437 NVGDCRTLLDTEYSSNSHHQRIPPSFR 517
V LDT Y + + +++ +++
Sbjct: 481 FVPRLENQLDTSYFDDFNDEQVLDAYK 507
>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 25.8 bits (54), Expect = 7.8
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +2
Query: 476 SSNSHHQRIPPSFRGCHSAASRQNFSACRF 565
S ++ +PP+F HS R++F C F
Sbjct: 241 SKSTPDNSLPPNFINNHSNVFRRSFHTCNF 270
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,043,933
Number of Sequences: 5004
Number of extensions: 57507
Number of successful extensions: 154
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -