Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_I07
(878 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35350.2 68417.m05022 cysteine endopeptidase, papain-type (XC... 47 2e-05
At4g35350.1 68417.m05023 cysteine endopeptidase, papain-type (XC... 47 2e-05
At2g27420.1 68415.m03314 cysteine proteinase, putative contains ... 45 8e-05
At1g29110.1 68414.m03563 cysteine proteinase, putative contains ... 45 8e-05
At4g23520.1 68417.m03390 cysteine proteinase, putative contains ... 44 1e-04
At2g34080.1 68415.m04172 cysteine proteinase, putative contains ... 44 1e-04
At1g29090.1 68414.m03561 peptidase C1A papain family protein con... 44 2e-04
At1g20850.1 68414.m02612 cysteine endopeptidase, papain-type (XC... 42 5e-04
At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP pr... 40 0.002
At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol pro... 39 0.005
At3g43960.1 68416.m04706 cysteine proteinase, putative contains ... 38 0.009
At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol p... 37 0.020
At4g11320.1 68417.m01828 cysteine proteinase, putative contains ... 36 0.036
At3g45310.1 68416.m04892 cysteine proteinase, putative similar t... 36 0.036
At4g16190.1 68417.m02457 cysteine proteinase, putative contains ... 36 0.047
At1g29080.1 68414.m03560 peptidase C1A papain family protein con... 34 0.14
At5g45890.1 68418.m05644 senescence-specific SAG12 protein (SAG1... 33 0.25
At3g48340.1 68416.m05276 cysteine proteinase, putative similar t... 31 1.3
At5g66210.2 68418.m08341 calcium-dependent protein kinase family... 30 1.8
At5g66210.1 68418.m08340 calcium-dependent protein kinase family... 30 1.8
At5g50260.1 68418.m06224 cysteine proteinase, putative similar t... 30 2.3
At2g38120.1 68415.m04679 amino acid permease, putative (AUX1) id... 29 5.4
At3g60290.1 68416.m06739 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 7.2
At3g48350.1 68416.m05277 cysteine proteinase, putative similar t... 28 9.5
>At4g35350.2 68417.m05022 cysteine endopeptidase, papain-type (XCP1)
identical to papain-type cysteine endopeptidase XCP1
GI:6708181 from [Arabidopsis thaliana]
Length = 288
Score = 46.8 bits (106), Expect = 2e-05
Identities = 30/97 (30%), Positives = 48/97 (49%), Gaps = 2/97 (2%)
Frame = +3
Query: 135 ALLIATVVMASSAEPXXPSHYA-LNQAKXLFEIFVKXHNREYKDDADRELHYQSFKKHLA 311
ALL S P H ++ LFE ++ H++ YK ++ ++ F+++L
Sbjct: 21 ALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLM 80
Query: 312 EINQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLP 419
I+Q N + G+N+FAD T EE + R LGL P
Sbjct: 81 HIDQRNNEINSYWLGLNEFADLTHEEFKGRYLGLAKP 117
>At4g35350.1 68417.m05023 cysteine endopeptidase, papain-type (XCP1)
identical to papain-type cysteine endopeptidase XCP1
GI:6708181 from [Arabidopsis thaliana]
Length = 355
Score = 46.8 bits (106), Expect = 2e-05
Identities = 30/97 (30%), Positives = 48/97 (49%), Gaps = 2/97 (2%)
Frame = +3
Query: 135 ALLIATVVMASSAEPXXPSHYA-LNQAKXLFEIFVKXHNREYKDDADRELHYQSFKKHLA 311
ALL S P H ++ LFE ++ H++ YK ++ ++ F+++L
Sbjct: 21 ALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLM 80
Query: 312 EINQLNEKNPYTTFGINKFADYTPEEQQSR-LGLRLP 419
I+Q N + G+N+FAD T EE + R LGL P
Sbjct: 81 HIDQRNNEINSYWLGLNEFADLTHEEFKGRYLGLAKP 117
>At2g27420.1 68415.m03314 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 348
Score = 44.8 bits (101), Expect = 8e-05
Identities = 26/84 (30%), Positives = 42/84 (50%), Gaps = 2/84 (2%)
Frame = +3
Query: 225 EIFVKXHNREYKDDADRELHYQSFKKHLAEINQLNEKNPYT-TFGINKFADYTPEE-QQS 398
E ++ NR Y D+ ++ + FKK+L + N N T IN+F+D T EE + +
Sbjct: 36 EQWMARFNRVYSDETEKRNRFNIFKKNLEFVQNFNMNNKITYKVDINEFSDLTDEEFRAT 95
Query: 399 RLGLRLPAKKT*VLRLQXYRNAIP 470
GL +P T + L +N +P
Sbjct: 96 HTGLVVPEAITRISTLSSGKNTVP 119
>At1g29110.1 68414.m03563 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 334
Score = 44.8 bits (101), Expect = 8e-05
Identities = 33/102 (32%), Positives = 54/102 (52%), Gaps = 5/102 (4%)
Frame = +3
Query: 126 VSVALLIATV-VMASSAEPXXPSHYALNQAKXL--FEIFVKXHNREYKDDADRELHYQSF 296
V VAL I ++ + S A P H LN+ + + ++ +R YKD++++E+ + F
Sbjct: 7 VFVALTILSMDLRISQARP----HVTLNEQSIVDYHQQWMTQFSRVYKDESEKEMRLKVF 62
Query: 297 KKHLAEINQLNEK-NPYTTFGINKFADYTPEE-QQSRLGLRL 416
KK+L I N N T G+N+F D+ EE + GLR+
Sbjct: 63 KKNLKFIENFNNMGNQSYTLGVNEFTDWKTEEFLATHTGLRV 104
>At4g23520.1 68417.m03390 cysteine proteinase, putative contains
similarity to cysteine proteinase (thiol protease) RD21A
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 356
Score = 44.4 bits (100), Expect = 1e-04
Identities = 28/90 (31%), Positives = 49/90 (54%), Gaps = 6/90 (6%)
Frame = +3
Query: 138 LLIATVVMASSAEPXXPS----HYALNQ-AKXLFEIFVKXHNREYKDD-ADRELHYQSFK 299
LLI V+ A S+ P+ H N+ + +F++++ H + Y + ++E +Q+FK
Sbjct: 14 LLIVFVLSAPSSAMDLPATSGGHNRSNEEVEFIFQMWMSKHGKTYTNALGEKERRFQNFK 73
Query: 300 KHLAEINQLNEKNPYTTFGINKFADYTPEE 389
+L I+Q N KN G+ +FAD T +E
Sbjct: 74 DNLRFIDQHNAKNLSYQLGLTRFADLTVQE 103
>At2g34080.1 68415.m04172 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 345
Score = 44.0 bits (99), Expect = 1e-04
Identities = 21/56 (37%), Positives = 34/56 (60%), Gaps = 1/56 (1%)
Frame = +3
Query: 225 EIFVKXHNREYKDDADRELHYQSFKKHLAEINQLNEK-NPYTTFGINKFADYTPEE 389
E ++ +REY+D+ ++ + FKK+L I N+K N G+N+FAD+T EE
Sbjct: 40 EQWMARFSREYRDELEKNMRRDVFKKNLKFIENFNKKGNKSYKLGVNEFADWTNEE 95
>At1g29090.1 68414.m03561 peptidase C1A papain family protein
contains similarity to cysteine protease SPCP1
GI:13491750 from [Ipomoea batatas]; contains Pfam
profile PF00112: Papain family cysteine protease
Length = 355
Score = 43.6 bits (98), Expect = 2e-04
Identities = 19/49 (38%), Positives = 32/49 (65%), Gaps = 1/49 (2%)
Frame = +3
Query: 246 NREYKDDADRELHYQSFKKHLAEINQLNEKNPYT-TFGINKFADYTPEE 389
+R Y D+ ++++ + FKK+L I + N+K T G+N+FAD+T EE
Sbjct: 55 SRVYSDELEKQMRFDVFKKNLKFIEKFNKKGDRTYKLGVNEFADWTREE 103
>At1g20850.1 68414.m02612 cysteine endopeptidase, papain-type (XCP2)
identical to papain-type cysteine endopeptidase XCP2
GI:6708183 from [Arabidopsis thaliana]
Length = 356
Score = 41.9 bits (94), Expect = 5e-04
Identities = 21/66 (31%), Positives = 39/66 (59%), Gaps = 1/66 (1%)
Frame = +3
Query: 219 LFEIFVKXHNREYKDDADRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEE-QQ 395
LFE ++ + Y+ ++ L ++ FK +L I++ N+K G+N+FAD + EE ++
Sbjct: 50 LFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKK 109
Query: 396 SRLGLR 413
LGL+
Sbjct: 110 MYLGLK 115
>At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP
protein (AALP) identical to AALP protein GI:7230640 from
[Arabidopsis thaliana]; similar to barley aleurain
Length = 358
Score = 39.9 bits (89), Expect = 0.002
Identities = 20/63 (31%), Positives = 37/63 (58%), Gaps = 1/63 (1%)
Frame = +3
Query: 222 FEIFVKXHNREYKDDADRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEE-QQS 398
F F + ++Y++ + +L + FK++L I N+K G+N+FAD T +E Q++
Sbjct: 59 FARFTHRYGKKYQNVEEMKLRFSIFKENLDLIRSTNKKGLSYKLGVNQFADLTWQEFQRT 118
Query: 399 RLG 407
+LG
Sbjct: 119 KLG 121
>At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol
protease identical to SP|P43297 Cysteine proteinase
RD21A precursor (EC 3.4.22.-) {Arabidopsis thaliana},
thiol protease RD21A SP:P43297 from [Arabidopsis
thaliana]
Length = 462
Score = 38.7 bits (86), Expect = 0.005
Identities = 19/72 (26%), Positives = 42/72 (58%), Gaps = 3/72 (4%)
Frame = +3
Query: 219 LFEIFVKXHNREYKDDA--DRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEEQ 392
++E ++ H + ++ +++ ++ FK +L +++ NEKN G+ +FAD T +E
Sbjct: 49 IYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEY 108
Query: 393 QSR-LGLRLPAK 425
+S+ LG ++ K
Sbjct: 109 RSKYLGAKMEKK 120
>At3g43960.1 68416.m04706 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 376
Score = 37.9 bits (84), Expect = 0.009
Identities = 34/128 (26%), Positives = 66/128 (51%), Gaps = 8/128 (6%)
Frame = +3
Query: 123 FVSVALLIATVVMAS-SAEPXXPSHYALNQAKXL--FEIFVKXHNREYKDDADRELHYQS 293
F ++ALL +V++ S S + N+ + L +E ++ + + Y ++E ++
Sbjct: 5 FRTLALLTLSVLLISISLGVVTATESQRNEGEVLTMYEQWLVENGKNYNGLGEKERRFKI 64
Query: 294 FKKHLAEINQLN-EKNPYTTFGINKFADYTPEE-QQSRLGLRLPAKK-T*VLRLQXYR-- 458
FK +L I + N + N G+NKF+D T +E Q S LG ++ K + V Y+
Sbjct: 65 FKDNLKRIEEHNSDPNRSYERGLNKFSDLTADEFQASYLGGKMEKKSLSDVAERYQYKEG 124
Query: 459 NAIPNEIN 482
+ +P+E++
Sbjct: 125 DVLPDEVD 132
>At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol
protease, putative similar to cysteine proteinase RD21A
precursor (thiol protease) GI:435619, SP:P43297 from
[Arabidopsis thaliana]
Length = 463
Score = 36.7 bits (81), Expect = 0.020
Identities = 22/80 (27%), Positives = 45/80 (56%), Gaps = 5/80 (6%)
Frame = +3
Query: 204 NQAKXLFEIFVKXHNREYKDD----ADRELHYQSFKKHLAEINQLNEKNPYTTFGINKFA 371
++ + ++E ++ H ++ + A+++ ++ FK +L I++ N KN G+ +FA
Sbjct: 44 SEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFA 103
Query: 372 DYTPEEQQSR-LGLRLPAKK 428
D T EE +S LG + P K+
Sbjct: 104 DLTNEEYRSMYLGAK-PTKR 122
>At4g11320.1 68417.m01828 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 371
Score = 35.9 bits (79), Expect = 0.036
Identities = 18/61 (29%), Positives = 31/61 (50%)
Frame = +3
Query: 207 QAKXLFEIFVKXHNREYKDDADRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPE 386
+A +FE ++ H + Y A++E F+ +L I N +N G+N+FAD +
Sbjct: 51 EATLMFESWMVKHGKVYDSVAEKERRLTIFEDNLRFITNRNAENLSYRLGLNRFADLSLH 110
Query: 387 E 389
E
Sbjct: 111 E 111
>At3g45310.1 68416.m04892 cysteine proteinase, putative similar to
AALP protein GI:7230640 from [Arabidopsis thaliana] and
barley aleurain
Length = 358
Score = 35.9 bits (79), Expect = 0.036
Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
Frame = +3
Query: 222 FEIFVKXHNREYKDDADRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEE-QQS 398
F F + ++Y+ + +L + FK++L I N+K +N+FAD T +E Q+
Sbjct: 59 FSRFTHRYGKKYQSVEEMKLRFSVFKENLDLIRSTNKKGLSYKLSLNQFADLTWQEFQRY 118
Query: 399 RLG 407
+LG
Sbjct: 119 KLG 121
>At4g16190.1 68417.m02457 cysteine proteinase, putative contains
similarity to papain-like cysteine proteinase isoform I
GI:7381219 from [Ipomoea batatas]
Length = 373
Score = 35.5 bits (78), Expect = 0.047
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Frame = +3
Query: 201 LNQAKXLFEIFVKXHNREYKDDADRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYT 380
L A+ F +F + + Y + + ++ FK +L + +P G+ +F+D T
Sbjct: 48 LLNAEHHFTLFKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLT 107
Query: 381 PEE-QQSRLGLR 413
P+E ++ LGL+
Sbjct: 108 PKEFRRKFLGLK 119
>At1g29080.1 68414.m03560 peptidase C1A papain family protein
contains similarity to cysteine protease SPCP1
GI:13491750 from [Ipomoea batatas]; contains Pfam
profile PF00112: Papain family cysteine protease
Length = 346
Score = 33.9 bits (74), Expect = 0.14
Identities = 23/92 (25%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
Frame = +3
Query: 117 MHFVSVALLIATVVMASSAEPXXPSHYALNQAKXLFEIFVKXHNREYKDDADRELHYQSF 296
+ FV V L I + + S + Y + + ++ +R Y D+ +++L Q
Sbjct: 4 VEFVCVVLTIFFMDLKISEATSRVALYKPSSIVDYHQQWMIQFSRVYDDEFEKQLRLQVL 63
Query: 297 KKHLAEINQLNEK-NPYTTFGINKFADYTPEE 389
++L I N N G+N+F D+T EE
Sbjct: 64 TENLKFIESFNNMGNQSYKLGVNEFTDWTKEE 95
>At5g45890.1 68418.m05644 senescence-specific SAG12 protein (SAG12)
/ cysteine proteinase, putative identical to
senescence-specific protein SAG12 GI:1046373 from
[Arabidopsis thaliana]
Length = 346
Score = 33.1 bits (72), Expect = 0.25
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +3
Query: 243 HNREYKDDADRELHYQSFKKHLAEINQLNEKNPYTTF--GINKFADYTPEEQQS 398
H R Y D + Y FK ++ I LN TF +N+FAD T +E +S
Sbjct: 45 HGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRS 98
>At3g48340.1 68416.m05276 cysteine proteinase, putative similar to
cysteine endopeptidase precursor [Ricinus communis]
GI:2944446; contains Pfam profile PF00112: Papain family
cysteine protease
Length = 351
Score = 30.7 bits (66), Expect = 1.3
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +3
Query: 270 DRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEE 389
+RE + F+ ++ ++ N+KN +NKFAD T E
Sbjct: 53 EREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINE 92
>At5g66210.2 68418.m08341 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 523
Score = 30.3 bits (65), Expect = 1.8
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +3
Query: 279 LHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEEQQSRLGLR 413
LH ++H +E QL + + F ++K TPEE + GLR
Sbjct: 432 LHVHQLEEHDSEKWQLRSRAAFEKFDLDKDGYITPEELRMHTGLR 476
>At5g66210.1 68418.m08340 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 523
Score = 30.3 bits (65), Expect = 1.8
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +3
Query: 279 LHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEEQQSRLGLR 413
LH ++H +E QL + + F ++K TPEE + GLR
Sbjct: 432 LHVHQLEEHDSEKWQLRSRAAFEKFDLDKDGYITPEELRMHTGLR 476
>At5g50260.1 68418.m06224 cysteine proteinase, putative similar to
cysteine endopeptidase precursor CysEP GI:2944446 from
[Ricinus communis]
Length = 361
Score = 29.9 bits (64), Expect = 2.3
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = +3
Query: 237 KXHNREYKDDADRELHYQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEE 389
+ H+ + ++ + FK ++ I++ N+K+ +NKF D T EE
Sbjct: 42 RSHHTVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEE 92
>At2g38120.1 68415.m04679 amino acid permease, putative (AUX1)
identical to AUX1 GI:1531758 from [Arabidopsis thaliana]
Length = 485
Score = 28.7 bits (61), Expect = 5.4
Identities = 23/77 (29%), Positives = 38/77 (49%), Gaps = 3/77 (3%)
Frame = -1
Query: 533 LLIIFDKF*TFYKQSQYIYFVWNSIPIXLKSQNLCL--LSRQPQSQPALLFLRSIISKFV 360
+L++ +F TF +YFVW + ++++CL L+R P P + FL I F
Sbjct: 316 ILMLIHQFITFGFACTPLYFVWEKVIGMHDTKSICLRALARLPVVIP-IWFLAIIFPFFG 374
Query: 359 NAKRSIWILLVQL-VYL 312
++ LLV VY+
Sbjct: 375 PINSAVGALLVSFTVYI 391
>At3g60290.1 68416.m06739 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to flavonol synthase 1
[SP|Q96330], gibberellin 20-oxidase [GI:9791186];
contains PF03171 2OG-Fe(II) oxygenase superfamily domain
Length = 316
Score = 28.3 bits (60), Expect = 7.2
Identities = 17/47 (36%), Positives = 22/47 (46%), Gaps = 3/47 (6%)
Frame = +3
Query: 246 NREYKDDADRELHYQSFKKHLAEINQL-NEKNP--YTTFGINKFADY 377
N++YK + LH K ++ QL NE P Y F N F DY
Sbjct: 250 NKDYKRLSFASLHSLPMHKKISPATQLVNENKPAAYGEFSFNDFLDY 296
>At3g48350.1 68416.m05277 cysteine proteinase, putative similar to
cysteine endopeptidase precursor [Ricinus communis]
GI:2944446; contains Pfam profile PF00112: Papain family
cysteine protease
Length = 364
Score = 27.9 bits (59), Expect = 9.5
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +3
Query: 285 YQSFKKHLAEINQLNEKNPYTTFGINKFADYTPEEQQS 398
+ F+ ++ +++ N+KN IN+FAD T E +S
Sbjct: 58 FNVFRHNVLHVHRTNKKNKPYKLKINRFADITHHEFRS 95
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,774,323
Number of Sequences: 28952
Number of extensions: 172711
Number of successful extensions: 353
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 347
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 350
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2067932800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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