Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_I04
         (866 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    28   1.5  
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    27   3.5  
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    27   4.6  
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    27   4.6  
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb...    26   6.0  
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa...    26   6.0  
SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr ...    26   8.0  
SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces pomb...    26   8.0  

>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 28.3 bits (60), Expect = 1.5
 Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
 Frame = +1

Query: 160 IKRPCPN-FDLNCIREYFSRNSQCQLVRGPVPDP 258
           I+ PC + F  NCI+ +   N  C + R PV DP
Sbjct: 410 IQLPCKHYFHENCIKPWLRVNGTCAICRAPV-DP 442


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
           domain|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1811

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 6/90 (6%)
 Frame = +1

Query: 352 IIEFYIN-----SKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLT 516
           +IE Y+N       TE +   A V   ++AS  T    +      + R D L +D     
Sbjct: 527 LIELYLNYDCISGNTENIYERAIVTLSRIASQST----SDPPPSFVFRDDQLVIDKPGF- 581

Query: 517 FTAVFPSISDL-QLSNAEVFSYVHEINPKF 603
              V+ +++D+ QL+++ + SYVH  NP +
Sbjct: 582 ---VYHTLNDIPQLNSSTIGSYVHSHNPPY 608


>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1158

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 25/109 (22%), Positives = 50/109 (45%)
 Frame = +1

Query: 391  VLAAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLTFTAVFPSISDLQLSNAEV 570
            +L   V+S  L +   I++ +R  K    ++ +L+  +G      +F   +++Q+   E 
Sbjct: 930  ILVCAVKSTVLNTTLRIYEADRALKNK--KTQSLKKPFGNQATLKIF---TEVQMP-MEA 983

Query: 571  FSYVHEINPKFILGPLLSFSLDSETQSQLGXLLNTSRLVFKKYLKRQEL 717
             S VH +  K  +G    F + S   +    LLN +   F+   KR+++
Sbjct: 984  LS-VHFLKTKLCVGSFKGFDIISLENAVFQSLLNPADTSFRFLEKREDI 1031


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +1

Query: 565  EVFSYVHEINPKFILGPLLSFSLDSETQSQLGXLLNTSRLVFK 693
            + + ++H IN  F+L  LLS SL +   SQ   LL    L+FK
Sbjct: 878  KTYGHLHYIN--FVLEKLLSSSLLTYNSSQRDKLLYEVSLLFK 918


>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 741

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 11/31 (35%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
 Frame = +1

Query: 163 KRPCPN-FDLNCIREYFSRNSQCQLVRGPVP 252
           K PC + F  NC++++   +  C L R  VP
Sbjct: 124 KMPCGHIFGKNCLQKWLENHCTCPLCRKEVP 154


>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
           Msa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 533

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 11/32 (34%), Positives = 15/32 (46%)
 Frame = +1

Query: 220 SQCQLVRGPVPDPLPLNYYRVYIPNSNLTVEY 315
           S   L  G VP  +P +YY +Y P   +   Y
Sbjct: 488 SMATLPPGAVPTSIPQSYYPIYSPEMAMPQSY 519


>SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 678

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +1

Query: 556 SNAEVFSYVHEINPKFILGPLLSFSLDS 639
           +N  + S V  +NP  ++ PLL  SLD+
Sbjct: 44  TNRIMLSVVSSLNPDSLIAPLLCVSLDN 71


>SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 561

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 10/30 (33%), Positives = 19/30 (63%)
 Frame = +1

Query: 253 DPLPLNYYRVYIPNSNLTVEYHDVKTRGFD 342
           +P  LNY+RV++P+  + +    ++ R FD
Sbjct: 486 EPHELNYFRVFLPSGKILIHRLQLRER-FD 514


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,179,197
Number of Sequences: 5004
Number of extensions: 62683
Number of successful extensions: 177
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)