BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_H24 (936 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.6 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 8.8 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 26.2 bits (55), Expect = 6.6 Identities = 13/37 (35%), Positives = 13/37 (35%) Frame = -2 Query: 332 PPXXXXPXFPFXPXXXXXXXPPXPPXPTXKXXXPPPP 222 PP P P P P PP P PPPP Sbjct: 1699 PPQMSAPTPP-PPPMSVPPPPSAPPMPAGPPSAPPPP 1734 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.8 bits (54), Expect = 8.8 Identities = 9/17 (52%), Positives = 9/17 (52%) Frame = -2 Query: 272 PPXPPXPTXKXXXPPPP 222 PP PP P PPPP Sbjct: 762 PPPPPPPGVAGAGPPPP 778 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 688,561 Number of Sequences: 5004 Number of extensions: 7164 Number of successful extensions: 39 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 35 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 475330268 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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