Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_G21
(869 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 33 0.040
SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyce... 32 0.12
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 29 0.86
SPBPB21E7.04c ||SPAPB21E7.04c, SPAPB21E7.04c|S-adenosylmethionin... 28 2.0
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 27 4.6
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 26 6.1
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 26 8.0
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 26 8.0
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 8.0
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 33.5 bits (73), Expect = 0.040
Identities = 15/60 (25%), Positives = 34/60 (56%), Gaps = 3/60 (5%)
Frame = +2
Query: 194 EYSTPASDPKNMQEVTQYV---QSLLQNMQDKFQSMSDQIINRIDEMGSRVDELEKNITD 364
EY+ PK ++E+T+ + +S L+ +KF+ S+ + +R+D + + +L+ I +
Sbjct: 335 EYTVDLETPKLVEELTKQLHVAESTLKENSEKFKQNSESLKSRVDNLNDYITKLQNEIDE 394
>SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 210
Score = 31.9 bits (69), Expect = 0.12
Identities = 19/70 (27%), Positives = 39/70 (55%), Gaps = 1/70 (1%)
Frame = +2
Query: 161 NPDNIVKNTENEYSTPASDPKNMQEVTQYVQSLLQNMQ-DKFQSMSDQIINRIDEMGSRV 337
N + TE+ +T A+ + MQE + ++S +N+ +K + + D+I + +D G
Sbjct: 88 NMEQAAMTTESLKNTMAT-VQTMQETARQLKSQSKNVSIEKIEKLQDEIQDYMDAAGELN 146
Query: 338 DELEKNITDL 367
+ L +N+TD+
Sbjct: 147 EVLGQNMTDI 156
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 29.1 bits (62), Expect = 0.86
Identities = 15/68 (22%), Positives = 33/68 (48%)
Frame = +2
Query: 221 KNMQEVTQYVQSLLQNMQDKFQSMSDQIINRIDEMGSRVDELEKNITDLMTQAGVENEK* 400
K +Q ++ LL+ Q F+ + + S+++ LEK ++ L +Q + NE+
Sbjct: 313 KELQNSNGELEKLLEAAQSSFEEQLESHKEAEASLKSQINFLEKEVSSLESQLKLANERL 372
Query: 401 K*FSSINL 424
+ + I +
Sbjct: 373 RHYDEIEI 380
>SPBPB21E7.04c ||SPAPB21E7.04c,
SPAPB21E7.04c|S-adenosylmethionine-dependent
methyltransferase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 281
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +2
Query: 230 QEVTQYVQSLLQNMQDKFQSMSDQIINRIDE 322
QE+ Y+ SL + D+ + D++IN IDE
Sbjct: 37 QELENYIFSLPREKLDQIRGKPDEVINVIDE 67
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 26.6 bits (56), Expect = 4.6
Identities = 15/74 (20%), Positives = 34/74 (45%)
Frame = +2
Query: 176 VKNTENEYSTPASDPKNMQEVTQYVQSLLQNMQDKFQSMSDQIINRIDEMGSRVDELEKN 355
++ + ++ +S + +E+ +S L + QS ++ +++ +V +LEK
Sbjct: 173 IEERKKQHMFASSSSRVKEEILVQEKSALVSDLASLQSDHSKVCEKLEVSSRQVQDLEKK 232
Query: 356 ITDLMTQAGVENEK 397
+ L Q NEK
Sbjct: 233 LAGLAQQNTELNEK 246
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/54 (24%), Positives = 31/54 (57%)
Frame = +2
Query: 233 EVTQYVQSLLQNMQDKFQSMSDQIINRIDEMGSRVDELEKNITDLMTQAGVENE 394
E+ + V++L +++ + Q++ QI N + E + + E KN+ + + ++NE
Sbjct: 551 ELREKVRALECDVEIQKQTVQYQISNAVKENSNTLSEQIKNLESELNSSKIKNE 604
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 26.2 bits (55), Expect = 6.1
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +2
Query: 242 QYVQSLLQNMQDKFQSMSDQIINRIDEMGSRVDELEK 352
+Y L + DK Q+ + NR+DE+ VD K
Sbjct: 483 EYESKNLLELLDKHQTALSSVENRLDEISEIVDSYHK 519
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 25.8 bits (54), Expect = 8.0
Identities = 9/37 (24%), Positives = 24/37 (64%)
Frame = +2
Query: 257 LLQNMQDKFQSMSDQIINRIDEMGSRVDELEKNITDL 367
+L+N+ ++ + +S++ + DE+ +D+LE+ + L
Sbjct: 123 ILENLDEQTRKLSERTEKQQDELDIALDDLEETLNTL 159
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +3
Query: 255 HFYKTCKINFKVCLIKS*IELMKWEAELMNWKRILQI--L*HKL 380
H+ I+ K+ I S + +++ +NWKR+ Q+ + HKL
Sbjct: 154 HYDLNAPIHAKLLTIGSSVSPIRFTLSSLNWKRLYQVQNICHKL 197
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 25.8 bits (54), Expect = 8.0
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +3
Query: 159 RTLTILLKTQKMNTQHQPAI 218
RT TI+ KT+ ++ HQPA+
Sbjct: 135 RTETIVKKTEHADSNHQPAV 154
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,178,600
Number of Sequences: 5004
Number of extensions: 65265
Number of successful extensions: 193
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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