BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_G20 (879 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g11090.1 68414.m01270 hydrolase, alpha/beta fold family prote... 31 1.3 At4g00234.1 68417.m00028 expressed protein 29 5.4 At4g00290.1 68417.m00036 mechanosensitive ion channel domain-con... 28 9.5 >At1g11090.1 68414.m01270 hydrolase, alpha/beta fold family protein similar to monoglyceride lipase from [Homo sapiens] GI:14594904, [Mus musculus] GI:2632162; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 324 Score = 30.7 bits (66), Expect = 1.3 Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Frame = +1 Query: 124 VFLVFVFARYVASDGDALLKP-CKLGDMQCLSSATXQFLEKTSKGIPQYDIWPIDPLVVT 300 + L+ FA + DG L+ P CK+ D QFL S+ +P + I P + L+ Sbjct: 144 ICLLIQFADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLIMISRFLPTWAIVPTEDLLEK 203 Query: 301 SLDV 312 S+ V Sbjct: 204 SIKV 207 >At4g00234.1 68417.m00028 expressed protein Length = 263 Score = 28.7 bits (61), Expect = 5.4 Identities = 20/83 (24%), Positives = 36/83 (43%) Frame = -2 Query: 464 MSPTICKSAFVFSSTVLALVSIWKSDICWFFRPVMFKFLKRITTPASFGAXTSNDVTTSG 285 ++PT + + FS TV V + + WF + R+ + SFG + V T Sbjct: 177 VAPTTIAAQY-FSPTVKGAVIL---SVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLD 232 Query: 284 SIGHMSYCGIPLLVFSKNCXVAL 216 + + I L+ ++ C VA+ Sbjct: 233 KVSSVGLFAIGLMASAEACGVAV 255 >At4g00290.1 68417.m00036 mechanosensitive ion channel domain-containing protein / MS ion channel domain-containing protein weak similarity to SP|P77338 Potassium efflux system kefA {Escherichia coli}; contains Pfam profile PF00924: Mechanosensitive ion channel Length = 497 Score = 27.9 bits (59), Expect = 9.5 Identities = 20/83 (24%), Positives = 35/83 (42%) Frame = -2 Query: 464 MSPTICKSAFVFSSTVLALVSIWKSDICWFFRPVMFKFLKRITTPASFGAXTSNDVTTSG 285 ++PT + + FS TV V + + WF + R+ + SFG V T Sbjct: 229 VAPTTIAAQY-FSPTVKGAVIL---SLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLD 284 Query: 284 SIGHMSYCGIPLLVFSKNCXVAL 216 + + I L+ ++ C VA+ Sbjct: 285 KVSSVGLFAIGLMASAEACGVAV 307 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,755,888 Number of Sequences: 28952 Number of extensions: 282417 Number of successful extensions: 540 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 525 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 540 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 2067932800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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