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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_G20
         (879 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g11090.1 68414.m01270 hydrolase, alpha/beta fold family prote...    31   1.3  
At4g00234.1 68417.m00028 expressed protein                             29   5.4  
At4g00290.1 68417.m00036 mechanosensitive ion channel domain-con...    28   9.5  

>At1g11090.1 68414.m01270 hydrolase, alpha/beta fold family protein
           similar to monoglyceride lipase from [Homo sapiens]
           GI:14594904, [Mus musculus] GI:2632162; contains Pfam
           profile PF00561: hydrolase, alpha/beta fold family
          Length = 324

 Score = 30.7 bits (66), Expect = 1.3
 Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
 Frame = +1

Query: 124 VFLVFVFARYVASDGDALLKP-CKLGDMQCLSSATXQFLEKTSKGIPQYDIWPIDPLVVT 300
           + L+  FA  +  DG  L+ P CK+ D         QFL   S+ +P + I P + L+  
Sbjct: 144 ICLLIQFADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLIMISRFLPTWAIVPTEDLLEK 203

Query: 301 SLDV 312
           S+ V
Sbjct: 204 SIKV 207


>At4g00234.1 68417.m00028 expressed protein
          Length = 263

 Score = 28.7 bits (61), Expect = 5.4
 Identities = 20/83 (24%), Positives = 36/83 (43%)
 Frame = -2

Query: 464 MSPTICKSAFVFSSTVLALVSIWKSDICWFFRPVMFKFLKRITTPASFGAXTSNDVTTSG 285
           ++PT   + + FS TV   V +    + WF        + R+ +  SFG    + V T  
Sbjct: 177 VAPTTIAAQY-FSPTVKGAVIL---SVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLD 232

Query: 284 SIGHMSYCGIPLLVFSKNCXVAL 216
            +  +    I L+  ++ C VA+
Sbjct: 233 KVSSVGLFAIGLMASAEACGVAV 255


>At4g00290.1 68417.m00036 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein weak similarity to SP|P77338
           Potassium efflux system kefA {Escherichia coli};
           contains Pfam profile PF00924: Mechanosensitive ion
           channel
          Length = 497

 Score = 27.9 bits (59), Expect = 9.5
 Identities = 20/83 (24%), Positives = 35/83 (42%)
 Frame = -2

Query: 464 MSPTICKSAFVFSSTVLALVSIWKSDICWFFRPVMFKFLKRITTPASFGAXTSNDVTTSG 285
           ++PT   + + FS TV   V +    + WF        + R+ +  SFG      V T  
Sbjct: 229 VAPTTIAAQY-FSPTVKGAVIL---SLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLD 284

Query: 284 SIGHMSYCGIPLLVFSKNCXVAL 216
            +  +    I L+  ++ C VA+
Sbjct: 285 KVSSVGLFAIGLMASAEACGVAV 307


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,755,888
Number of Sequences: 28952
Number of extensions: 282417
Number of successful extensions: 540
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 525
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 540
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2067932800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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