Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_G16
         (874 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ...    88   2e-18
SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz...    33   0.070
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz...    32   0.093
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ...    29   0.86 
SPCC285.05 |||purine nucleoside transporter |Schizosaccharomyces...    26   8.1  

>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1012

 Score = 87.8 bits (208), Expect = 2e-18
 Identities = 58/150 (38%), Positives = 82/150 (54%), Gaps = 1/150 (0%)
 Frame = +3

Query: 279 EDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVIL-GWCXVGNFA*RQ 455
           ++ IDE LYSRQLYVLGH+AM++M+ S+VLI G  GLGVEIAKNV L G   V  +  + 
Sbjct: 12  QNTIDEGLYSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQP 71

Query: 456 KLHRG*SFLAVLPFRDCNWSEQGFNIVRTLSELNHYVPTTAYTGPLTEDFWRKFRVVVLT 635
                 S    L   D         + + L+ELN YVP +     L+ ++ + F+ VV+T
Sbjct: 72  TRIEDLSSQYFLTEDDIGVPRAKVTVSK-LAELNQYVPVSV-VDELSTEYLKNFKCVVVT 129

Query: 636 GASWAXXXXXXAFLXPNNIXWVIADTKXLF 725
             S         F   N+I ++ AD++ LF
Sbjct: 130 ETSLTKQLEINDFTHKNHIAYIAADSRGLF 159



 Score = 39.5 bits (88), Expect = 6e-04
 Identities = 19/40 (47%), Positives = 27/40 (67%)
 Frame = +1

Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKA 525
           K + L GV SVTL+D +   + DLSSQ++L+E  IG  +A
Sbjct: 54  KNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTEDDIGVPRA 93


>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
           Rad31|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 307

 Score = 32.7 bits (71), Expect = 0.070
 Identities = 21/55 (38%), Positives = 31/55 (56%)
 Frame = +3

Query: 255 MANNGARVEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVIL 419
           M N+    E EI  +LY RQ+ + G +A + +  S VL+     L  EIAKN++L
Sbjct: 1   MGNHNINAE-EI--ALYDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKNLVL 52



 Score = 25.8 bits (54), Expect = 8.1
 Identities = 12/40 (30%), Positives = 20/40 (50%)
 Frame = +1

Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKA 525
           K L L G+  + + D       D+  QF++  + IGQ +A
Sbjct: 48  KNLVLSGIGKLCVLDSMTVYEKDVEEQFFIEASDIGQLRA 87


>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
           Fub2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 628

 Score = 32.3 bits (70), Expect = 0.093
 Identities = 12/29 (41%), Positives = 21/29 (72%)
 Frame = +3

Query: 333 DAMRRMASSDVLISGLGGLGVEIAKNVIL 419
           +A+R   S+ VL+ G GG+G E+ KN+++
Sbjct: 18  EALRNFKSAKVLLVGAGGIGCELLKNLLM 46


>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 485

 Score = 29.1 bits (62), Expect = 0.86
 Identities = 12/44 (27%), Positives = 22/44 (50%)
 Frame = +3

Query: 261 NNGARVEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLG 392
           + G   ++ +     +R     G D M R+ +S V++ G GG+G
Sbjct: 95  SKGVPYDENLIREQLARNYAFFGEDGMERLRNSFVIVVGCGGVG 138


>SPCC285.05 |||purine nucleoside transporter |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 348

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 15/38 (39%), Positives = 20/38 (52%)
 Frame = -2

Query: 165 TSGTNAHNFARILVALTIKKKFSSNCLKQTFVLXQGAA 52
           T+G    N A  ++ALT+  KF    L +TF L  G A
Sbjct: 83  TTGEGKSNAASSIMALTLSPKFD---LTETFFLISGIA 117


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,244,743
Number of Sequences: 5004
Number of extensions: 60790
Number of successful extensions: 168
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)