Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_G16
(874 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 71 8e-13
At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 64 1e-10
At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 44 1e-04
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 44 1e-04
At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly... 44 1e-04
At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident... 43 3e-04
At2g32410.1 68415.m03960 auxin-resistance protein, putative stro... 38 0.009
At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) id... 37 0.020
At5g37530.1 68418.m04520 thiF family protein similar to SP|P3013... 33 0.19
At4g16160.2 68417.m02453 mitochondrial import inner membrane tra... 30 2.3
At4g16160.1 68417.m02452 mitochondrial import inner membrane tra... 30 2.3
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 30 2.3
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 30 2.3
At1g21430.1 68414.m02680 flavin-containing monooxygenase family ... 29 5.4
>At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1;
identical to gi:1703477
Length = 1077
Score = 71.3 bits (167), Expect = 8e-13
Identities = 53/150 (35%), Positives = 74/150 (49%), Gaps = 3/150 (2%)
Frame = +3
Query: 285 EIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGWCXVGNFA*RQKLH 464
EIDE L+SRQL V G + MR++ +S+VLISG+ GLGVEIAKN+IL +
Sbjct: 69 EIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVTLHDENVVE 128
Query: 465 R-G*SFLAVLPFRDCNWSEQGFNIVRTLSELNHYVPTTAYTGPLTEDFWRKFRVVVLTGA 641
S V D + V L ELN+ V + TG LT++ F+VVV
Sbjct: 129 LWDLSSNFVFTEEDIG-KNRALASVHKLQELNNAVAVSTLTGKLTKEQLSDFQVVVFVDI 187
Query: 642 SWAXXXXXXAFLXPNN--IXWVIADTKXLF 725
S+ + + I ++ AD + LF
Sbjct: 188 SFEKATEIDDYCHSHQPPIAFIKADVRGLF 217
Score = 51.6 bits (118), Expect = 7e-07
Identities = 46/128 (35%), Positives = 58/128 (45%), Gaps = 5/128 (3%)
Frame = +1
Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKALTSCEHF--LN*TTMCPLL- 576
K + L GV SVTLHD+ + DLSS F +E IG+N+AL S LN L
Sbjct: 109 KNIILAGVKSVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLT 168
Query: 577 --LTQDX*LKTFGVSSVLWSSQEHRGLXXXXXLRSSPPITXHGSLLILXXCSHXXFCDFG 750
LT++ L F V + S E PPI + + FCDFG
Sbjct: 169 GKLTKEQ-LSDFQVVVFVDISFEKATEIDDYCHSHQPPIAFIKA--DVRGLFGSLFCDFG 225
Query: 751 PXFTVLNV 774
P FTVL+V
Sbjct: 226 PHFTVLDV 233
>At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1;
identical to GB:U80808
Length = 1080
Score = 64.1 bits (149), Expect = 1e-10
Identities = 49/149 (32%), Positives = 73/149 (48%), Gaps = 2/149 (1%)
Frame = +3
Query: 285 EIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGWCXVGNFA*RQKLH 464
EIDE L+SRQL V G + MRR+ +S+VLISG+ GLG EIAKN+IL + +
Sbjct: 72 EIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGVKSVTLHDERVVE 131
Query: 465 RG*SFLAVLPFRDCNWSEQGFNIVRTLSELNHYVPTTAYTGPLTEDFWRKFRVVVLTGAS 644
+ D + V+ L +LN+ V ++ T L ++ F+VVV + S
Sbjct: 132 LWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNKEDLSGFQVVVFSDIS 191
Query: 645 WAXXXXXXAFLXPNN--IXWVIADTKXLF 725
+ + I +V AD + LF
Sbjct: 192 MERAIEFDDYCHSHQPPIAFVKADVRGLF 220
Score = 50.4 bits (115), Expect = 2e-06
Identities = 43/127 (33%), Positives = 57/127 (44%), Gaps = 4/127 (3%)
Frame = +1
Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKALTSCEHF--LN*TTMCPLLL 579
K L L GV SVTLHD++ + DLSS F SE +G+N+A S + LN + L
Sbjct: 112 KNLILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLT 171
Query: 580 TQDX*LKTFGVSSVLWSS-QEHRGLXXXXXLRS-SPPITXHGSLLILXXCSHXXFCDFGP 753
G V++S R + S PPI + + FCDFGP
Sbjct: 172 KSLNKEDLSGFQVVVFSDISMERAIEFDDYCHSHQPPIAFVKA--DVRGLFGSVFCDFGP 229
Query: 754 XFTVLNV 774
F VL+V
Sbjct: 230 EFAVLDV 236
>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50580;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 44.0 bits (99), Expect = 1e-04
Identities = 20/45 (44%), Positives = 29/45 (64%)
Frame = +3
Query: 285 EIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVIL 419
E + +LY RQ+ V G A RR++ S VL+SG+ G E KN++L
Sbjct: 8 EQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVL 52
>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 44.0 bits (99), Expect = 1e-04
Identities = 20/45 (44%), Positives = 29/45 (64%)
Frame = +3
Query: 285 EIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVIL 419
E + +LY RQ+ V G A RR++ S VL+SG+ G E KN++L
Sbjct: 8 EQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVL 52
>At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 318
Score = 44.0 bits (99), Expect = 1e-04
Identities = 20/45 (44%), Positives = 29/45 (64%)
Frame = +3
Query: 285 EIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVIL 419
E + +LY RQ+ V G A RR++ S VL+SG+ G E KN++L
Sbjct: 8 EQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVL 52
>At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a)
identical to SUMO activating enzyme 1a [Arabidopsis
thaliana] GI:22652850; contains Pfam profile PF00899:
ThiF family
Length = 322
Score = 42.7 bits (96), Expect = 3e-04
Identities = 18/45 (40%), Positives = 29/45 (64%)
Frame = +3
Query: 285 EIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVIL 419
E + +LY RQ+ V G +A RR+ + +L+SG+ G E KN++L
Sbjct: 8 EQETALYDRQIRVWGANAQRRLTKAHILVSGIKGTVAEFCKNIVL 52
>At2g32410.1 68415.m03960 auxin-resistance protein, putative strong
similarity to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family
Length = 523
Score = 37.9 bits (84), Expect = 0.009
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +1
Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKALTSC 537
K L +GG+ S+T+ D + DL + F + +GQ++A T C
Sbjct: 42 KNLVIGGIGSITIVDGSKVEIGDLGNNFMVDAKSVGQSRAKTVC 85
>At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 540
Score = 36.7 bits (81), Expect = 0.020
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +1
Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKALTSC 537
K L LGGV S+T+ D DL + F + +GQ+KA + C
Sbjct: 58 KNLVLGGVGSITVVDGSKVQFGDLGNNFMVDAKSVGQSKAKSVC 101
Score = 27.9 bits (59), Expect = 9.4
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +3
Query: 303 YSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILG 422
Y RQL + G + + + + G G E KN++LG
Sbjct: 24 YDRQLRIWGEVGQAALEEASICLLNCGPTGSEALKNLVLG 63
>At5g37530.1 68418.m04520 thiF family protein similar to SP|P30138
Adenylyltransferase thiF (EC 2.7.7.-) {Escherichia
coli}; contains Pfam profile PF00899: ThiF family
Length = 457
Score = 33.5 bits (73), Expect = 0.19
Identities = 16/62 (25%), Positives = 32/62 (51%)
Frame = +3
Query: 231 EAHSNNSAMANNGARVEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKN 410
E+ + +AN ++DEI +R + G ++ ++ S V++ GLGG+G A
Sbjct: 52 ESETRPDTVANGQDLLKDEIVSEHLTRNIQFFGLESQHKVTGSYVVVIGLGGVGSHAASM 111
Query: 411 VI 416
++
Sbjct: 112 LL 113
>At4g16160.2 68417.m02453 mitochondrial import inner membrane
translocase subunit Tim17/Tim22/Tim23 family protein
contains Pfam PF02466: Mitochondrial import inner
membrane translocase subunit Tim17
Length = 178
Score = 29.9 bits (64), Expect = 2.3
Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = +3
Query: 255 MANNGAR-VEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGL 389
M +G R V DEI S L+ LGH + R+A S V +G+G L
Sbjct: 1 MEKSGGRIVMDEI-RSFEKAHLFDLGHPLLNRIADSFVKAAGVGAL 45
>At4g16160.1 68417.m02452 mitochondrial import inner membrane
translocase subunit Tim17/Tim22/Tim23 family protein
contains Pfam PF02466: Mitochondrial import inner
membrane translocase subunit Tim17
Length = 176
Score = 29.9 bits (64), Expect = 2.3
Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = +3
Query: 255 MANNGAR-VEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGL 389
M +G R V DEI S L+ LGH + R+A S V +G+G L
Sbjct: 1 MEKSGGRIVMDEI-RSFEKAHLFDLGHPLLNRIADSFVKAAGVGAL 45
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 29.9 bits (64), Expect = 2.3
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +1
Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKA 525
KTL+L G + + D V++L+ QF + +GQ+KA
Sbjct: 29 KTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKA 68
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 29.9 bits (64), Expect = 2.3
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +1
Query: 406 KTLSLGGVXSVTLHDDKNCTVADLSSQFYLSETVIGQNKA 525
KTL+L G + + D V++L+ QF + +GQ+KA
Sbjct: 29 KTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKA 68
>At1g21430.1 68414.m02680 flavin-containing monooxygenase family
protein / FMO family protein similar to
flavin-containing monooxygenases YUCCA [gi:16555352],
YUCCA2 [gi:16555354], and YUCCA3 [gi:16555356] from
Arabidopsis thaliana
Length = 391
Score = 28.7 bits (61), Expect = 5.4
Identities = 19/55 (34%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = +3
Query: 249 SAMANNGARVEDEID---ESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIA 404
+A NG V EI ES + L+ + + A DVL+ G G G+EIA
Sbjct: 135 AATGENGEGVIPEIPGLVESFQGKYLHSSEYKNGEKFAGKDVLVVGCGNSGMEIA 189
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,411,632
Number of Sequences: 28952
Number of extensions: 338198
Number of successful extensions: 897
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 861
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 897
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2048424000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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