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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_F23
         (899 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q9V3U6 Cluster: CG8947-PA; n=22; Eumetazoa|Rep: CG8947-...    71   4e-11
UniRef50_UPI00015B60C0 Cluster: PREDICTED: similar to homologue ...    69   2e-10
UniRef50_Q86GZ5 Cluster: Midgut cysteine proteinase 2; n=1; Rhip...    56   9e-07
UniRef50_A7RWG6 Cluster: Predicted protein; n=3; Nematostella ve...    51   3e-05
UniRef50_UPI0000F20A8D Cluster: PREDICTED: hypothetical protein;...    46   0.001
UniRef50_UPI00015A56AC Cluster: hypothetical protein LOC550326; ...    45   0.003
UniRef50_Q6DGW1 Cluster: 26-29kD-proteinase protein; n=23; Danio...    44   0.004
UniRef50_A7SM85 Cluster: Predicted protein; n=2; Nematostella ve...    42   0.021
UniRef50_UPI0000E4978C Cluster: PREDICTED: similar to cathepsin ...    38   0.46 
UniRef50_UPI0000E4A10C Cluster: PREDICTED: similar to Lipase A, ...    36   1.1  
UniRef50_Q54TR1 Cluster: Counting factor associated protein; n=1...    33   7.5  

>UniRef50_Q9V3U6 Cluster: CG8947-PA; n=22; Eumetazoa|Rep: CG8947-PA
           - Drosophila melanogaster (Fruit fly)
          Length = 549

 Score = 70.9 bits (166), Expect = 4e-11
 Identities = 30/50 (60%), Positives = 35/50 (70%)
 Frame = +2

Query: 122 SPPQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQ 271
           +PP+W      + +L IPYAE+ EPFYAWYD     SRIDYYGGMV TYQ
Sbjct: 21  NPPKWDPNYIVKGTLYIPYAEIAEPFYAWYDKNTRRSRIDYYGGMVKTYQ 70


>UniRef50_UPI00015B60C0 Cluster: PREDICTED: similar to homologue of
           Sarcophaga 26,29kDa proteinase; n=1; Nasonia
           vitripennis|Rep: PREDICTED: similar to homologue of
           Sarcophaga 26,29kDa proteinase - Nasonia vitripennis
          Length = 553

 Score = 68.9 bits (161), Expect = 2e-10
 Identities = 30/49 (61%), Positives = 36/49 (73%)
 Frame = +2

Query: 155 RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQFTSAVYPPYG 301
           + +L IPYAE+ EPFYAWYD++   SRIDYYGGMV TYQ +     PYG
Sbjct: 35  KGTLYIPYAEIREPFYAWYDAQSGNSRIDYYGGMVKTYQLSKE--GPYG 81


>UniRef50_Q86GZ5 Cluster: Midgut cysteine proteinase 2; n=1;
           Rhipicephalus appendiculatus|Rep: Midgut cysteine
           proteinase 2 - Rhipicephalus appendiculatus (Brown ear
           tick)
          Length = 564

 Score = 56.4 bits (130), Expect = 9e-07
 Identities = 23/50 (46%), Positives = 32/50 (64%)
 Frame = +2

Query: 128 PQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQFT 277
           P W  + + +  L +PYAE+ EPF  +YD+  + SRIDYY GMV T Q +
Sbjct: 22  PDWGSFYKVKGVLYLPYAEIREPFTGYYDATQNTSRIDYYDGMVQTVQLS 71


>UniRef50_A7RWG6 Cluster: Predicted protein; n=3; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 513

 Score = 51.2 bits (117), Expect = 3e-05
 Identities = 20/36 (55%), Positives = 24/36 (66%)
 Frame = +2

Query: 164 LNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQ 271
           L++PY ++ EPF  WY      SRIDYYGGM  TYQ
Sbjct: 7   LSLPYGDIKEPFEVWYSGLHGMSRIDYYGGMDRTYQ 42


>UniRef50_UPI0000F20A8D Cluster: PREDICTED: hypothetical protein;
           n=1; Danio rerio|Rep: PREDICTED: hypothetical protein -
           Danio rerio
          Length = 150

 Score = 46.4 bits (105), Expect = 0.001
 Identities = 19/40 (47%), Positives = 26/40 (65%)
 Frame = +2

Query: 128 PQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYY 247
           P++ +    +  L++PYAE+ EPF AWYD     SRIDYY
Sbjct: 25  PEFGKTYHVKGLLSLPYAEIKEPFEAWYDLTGKRSRIDYY 64



 Score = 44.4 bits (100), Expect = 0.004
 Identities = 18/48 (37%), Positives = 29/48 (60%)
 Frame = +2

Query: 128 PQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQ 271
           P + +    +  L++P++++ EPF AWYD   + SRIDY    V T+Q
Sbjct: 76  PDFGKMYHVKGVLSLPHSKIEEPFEAWYDLDGNRSRIDYRNSTVRTFQ 123


>UniRef50_UPI00015A56AC Cluster: hypothetical protein LOC550326;
           n=2; Danio rerio|Rep: hypothetical protein LOC550326 -
           Danio rerio
          Length = 531

 Score = 44.8 bits (101), Expect = 0.003
 Identities = 25/82 (30%), Positives = 41/82 (50%), Gaps = 5/82 (6%)
 Frame = +2

Query: 128 PQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQFTS-----AVYP 292
           P + +    +  L++P++++ EPF AWYD   + SRIDY    V T+Q  +     A+Y 
Sbjct: 14  PDFGKMYHVKGVLSLPHSKIEEPFEAWYDLDGNRSRIDYRNSTVRTFQIGNDLDFGAIYK 73

Query: 293 PYGXL*RSLXSXXKPKXTXDXP 358
               +  S+    + K T D P
Sbjct: 74  ITPVIPPSVIKCFQLKGTKDDP 95


>UniRef50_Q6DGW1 Cluster: 26-29kD-proteinase protein; n=23; Danio
           rerio|Rep: 26-29kD-proteinase protein - Danio rerio
           (Zebrafish) (Brachydanio rerio)
          Length = 327

 Score = 44.4 bits (100), Expect = 0.004
 Identities = 18/48 (37%), Positives = 29/48 (60%)
 Frame = +2

Query: 128 PQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQ 271
           P + +    +  L++P++++ EPF AWYD   + SRIDY    V T+Q
Sbjct: 40  PDFGKMYHVKGVLSLPHSKIEEPFEAWYDLDGNRSRIDYRNSTVRTFQ 87


>UniRef50_A7SM85 Cluster: Predicted protein; n=2; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 514

 Score = 41.9 bits (94), Expect = 0.021
 Identities = 15/36 (41%), Positives = 25/36 (69%)
 Frame = +2

Query: 164 LNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQ 271
           L +P++++ EPF  W+ ++ + SRIDYY G   T+Q
Sbjct: 16  LQLPHSKIEEPFEVWFSAQHNRSRIDYYYGTDRTFQ 51


>UniRef50_UPI0000E4978C Cluster: PREDICTED: similar to cathepsin l;
           n=2; Strongylocentrotus purpuratus|Rep: PREDICTED:
           similar to cathepsin l - Strongylocentrotus purpuratus
          Length = 489

 Score = 37.5 bits (83), Expect = 0.46
 Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
 Frame = +2

Query: 77  LCFFVAFASGYVDKGSP--PQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYG 250
           L  F+  AS    + SP  P +  +      + +PYAEL EPF  ++    + SR+D Y 
Sbjct: 8   LVCFILVASLASLQASPVKPVFKDHYHASGQIRLPYAELIEPFEIFFQGPKNRSRMDTYS 67

Query: 251 GMVXTY 268
           GM   Y
Sbjct: 68  GMDKVY 73


>UniRef50_UPI0000E4A10C Cluster: PREDICTED: similar to Lipase A,
           lysosomal acid, cholesterol esterase (Wolman disease);
           n=4; Strongylocentrotus purpuratus|Rep: PREDICTED:
           similar to Lipase A, lysosomal acid, cholesterol
           esterase (Wolman disease) - Strongylocentrotus
           purpuratus
          Length = 525

 Score = 36.3 bits (80), Expect = 1.1
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = +2

Query: 164 LNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTY 268
           + +PYAEL EPF  ++    + SR+D Y GM   Y
Sbjct: 350 IRLPYAELIEPFEIFFQGPKNRSRMDTYSGMDKVY 384


>UniRef50_Q54TR1 Cluster: Counting factor associated protein; n=1;
           Dictyostelium discoideum AX4|Rep: Counting factor
           associated protein - Dictyostelium discoideum AX4
          Length = 531

 Score = 33.5 bits (73), Expect = 7.5
 Identities = 19/49 (38%), Positives = 22/49 (44%)
 Frame = +2

Query: 128 PQWSQYTQ*RDSLNIPYAELHEPFYAWYDSKXSXSRIDYYGGMVXTYQF 274
           P   QY   + S NIPY  + EP    YDS  +   I  Y GM  T  F
Sbjct: 23  PAAQQYYM-KGSFNIPYFNIVEPIELIYDSVNNRQYISVYNGMDITINF 70


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 387,253,087
Number of Sequences: 1657284
Number of extensions: 6087001
Number of successful extensions: 10943
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 10754
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10941
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 81571813589
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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