Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_F11
         (922 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q6URH4 Cluster: Juvenile hormone diol kinase; n=2; Obte...   140   6e-32
UniRef50_O16158 Cluster: CG14904-PA; n=9; Endopterygota|Rep: CG1...   136   1e-30
UniRef50_UPI00015B48AE Cluster: PREDICTED: similar to conserved ...   125   1e-27
UniRef50_O76730 Cluster: Calexcitin; n=4; Decapodiformes|Rep: Ca...    80   9e-14
UniRef50_P04570 Cluster: Sarcoplasmic calcium-binding proteins I...    52   2e-05
UniRef50_Q4C9W1 Cluster: Calcium-binding EF-hand; n=1; Crocospha...    48   3e-04
UniRef50_P45961 Cluster: Putative calcium-binding protein cex-2;...    38   0.48 
UniRef50_UPI0000DB6F98 Cluster: PREDICTED: hypothetical protein;...    35   2.5  

>UniRef50_Q6URH4 Cluster: Juvenile hormone diol kinase; n=2;
           Obtectomera|Rep: Juvenile hormone diol kinase - Bombyx
           mori (Silk moth)
          Length = 183

 Score =  140 bits (338), Expect = 6e-32
 Identities = 67/180 (37%), Positives = 101/180 (56%), Gaps = 4/180 (2%)
 Frame = +1

Query: 112 MVXDFRKXKLLHVXXXXFXXNGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTLLK 291
           MV + RK KLLHV    F  +  G+++K+DFELA + I+K RGW+ G   Y  +Q +++ 
Sbjct: 1   MVSEVRKKKLLHVFTVFFDSDKSGVVEKQDFELAAQNIAKLRGWAPGSPAYDILQESMIA 60

Query: 292 VWDGL-SXAXXDXXGQXSKEEWISLWEKFXXXPS---XWXNLXXKFIFQXEDXSNXGXIX 459
           +W GL   A  D  G+ +++EW++LW+++   P+    W NL  K IFQ +D SN G + 
Sbjct: 61  IWLGLQKQADADGDGKVTQDEWLALWDEYAKDPAAAKDWQNLLCKSIFQIQDSSNDGSVD 120

Query: 460 XEEFSXVXAXFGLXKXEXXXXFXKXXKGKXXVXXXEXXELXKEFXXXEDVXXPGXFVXGK 639
             E+  V   FGL K E    F K  KGK  +   +  EL KE+   +D   PG ++ G+
Sbjct: 121 VNEYVTVHESFGLNKEESTEAFKKLAKGKDSISWADFQELWKEYFSSDDPDVPGNYIFGR 180


>UniRef50_O16158 Cluster: CG14904-PA; n=9; Endopterygota|Rep:
           CG14904-PA - Drosophila melanogaster (Fruit fly)
          Length = 184

 Score =  136 bits (328), Expect = 1e-30
 Identities = 70/179 (39%), Positives = 95/179 (53%), Gaps = 4/179 (2%)
 Frame = +1

Query: 115 VXDFRKXKLLHVXXXXFXXNGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTLLKV 294
           + DFRK KLL +    F  N  G ID KDFELAIER+ + RGW     + KE    ++++
Sbjct: 3   ISDFRKKKLLFLFNVFFDVNQSGEIDVKDFELAIERVCQLRGWQKDTPKNKETYDLMMEI 62

Query: 295 WDGL-SXAXXDXXGQXSKEEWISLWEKFXXXPS---XWXNLXXKFIFQXEDXSNXGXIXX 462
           W GL S A  D  GQ S +EW ++W+ +   PS    W N    F+F  ED S+ G I  
Sbjct: 63  WTGLRSKADKDNDGQVSVDEWCNMWDAYAKDPSSVMDWQNAYMNFMFDLEDASHDGGIDV 122

Query: 463 EEFSXVXAXFGLXKXEXXXXFXKXXKGKXXVXXXEXXELXKEFXXXEDVXXPGXFVXGK 639
            EF+ V + +GL K E    F K  +G+  V   +   L KE+   EDV  PG ++ GK
Sbjct: 123 TEFTLVCSSYGLEKTECEEAFAKMSQGQSEVTREQFAALWKEYFAAEDVNAPGNYIFGK 181


>UniRef50_UPI00015B48AE Cluster: PREDICTED: similar to conserved
           hypothetical protein; n=1; Nasonia vitripennis|Rep:
           PREDICTED: similar to conserved hypothetical protein -
           Nasonia vitripennis
          Length = 188

 Score =  125 bits (302), Expect = 1e-27
 Identities = 70/182 (38%), Positives = 95/182 (52%), Gaps = 9/182 (4%)
 Frame = +1

Query: 121 DFRKXKLLHVXXXXFXX-----NGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTL 285
           +FRK KLL V    F       N  G ID KDF+LA+ERI ++RGW+AG  ++K+ + TL
Sbjct: 5   EFRKKKLLFVFNTFFDGIFFNVNQSGSIDIKDFDLAVERICEARGWAAGHPRFKQTKETL 64

Query: 286 LKVWDGL-SXAXXDXXGQXSKEEWISLWEKFXXXPS---XWXNLXXKFIFQXEDXSNXGX 453
            KVWDGL   A  D  GQ S++EW S+WE++   P     W       +F  ED S  G 
Sbjct: 65  NKVWDGLQKRADVDNDGQISRDEWYSMWEEYAKDPEHAVEWQQTYMNLVFDLEDTSGDGS 124

Query: 454 IXXEEFSXVXAXFGLXKXEXXXXFXKXXKGKXXVXXXEXXELXKEFXXXEDVXXPGXFVX 633
           I   EFS V   +G+ + E    F K   G   V   +  +L ++F   +D   PG F+ 
Sbjct: 125 IDEAEFSQVCRSYGVDESESREAFKKLQVGN-EVTRDKFEKLWQQFFSTDDPSTPGNFIF 183

Query: 634 GK 639
           GK
Sbjct: 184 GK 185


>UniRef50_O76730 Cluster: Calexcitin; n=4; Decapodiformes|Rep:
           Calexcitin - Todarodes pacificus (Japanese flying squid)
          Length = 191

 Score = 79.8 bits (188), Expect = 9e-14
 Identities = 52/181 (28%), Positives = 76/181 (41%), Gaps = 9/181 (4%)
 Frame = +1

Query: 121 DFRKXKLLHVXXXXFXXNGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTLLKVWD 300
           DF+K K+L V    +  N  G+I+  DFELAI++I     W     ++ E + TL  +WD
Sbjct: 8   DFQKSKILRVFNTFYDCNHDGVIEWGDFELAIKKICDLHSWPIDGKKHNEARATLKLIWD 67

Query: 301 GL-SXAXXDXXGQXSKEEWISLW-------EKFXXXPSXWXNLXXKFIFQXEDXSNXGXI 456
           GL   A  +   Q ++EEW+ +W       EK    P  W      F+F   D S    I
Sbjct: 68  GLRKYADENEDEQVTQEEWLKMWAECVKSVEKGDSLPE-WLTKYMNFMFDVNDTSGDNII 126

Query: 457 XXEEFSXVXAXFGLXKXEXXXXFXKXXK-GKXXVXXXEXXELXKEFXXXEDVXXPGXFVX 633
              E++ V   +G+ K +    F      GK  V       L  E+    D    G  + 
Sbjct: 127 DKREYTTVYTSYGISKVDCEAAFDTLSDGGKTMVTREIFARLWTEYFVSNDRAAKGNNLF 186

Query: 634 G 636
           G
Sbjct: 187 G 187


>UniRef50_P04570 Cluster: Sarcoplasmic calcium-binding proteins II,
           V, VI, and VII; n=3; Branchiostoma|Rep: Sarcoplasmic
           calcium-binding proteins II, V, VI, and VII -
           Branchiostoma lanceolatum (Common lancelet) (Amphioxus)
          Length = 185

 Score = 52.4 bits (120), Expect = 2e-05
 Identities = 38/133 (28%), Positives = 52/133 (39%), Gaps = 7/133 (5%)
 Frame = +1

Query: 121 DFRKXKLLHVXXXXFXXNGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTLLKVWD 300
           DF+K K+          N  G I   DFE  + R  +    S  DA YK +Q +L   W 
Sbjct: 4   DFQKQKIKFTFDFFLDMNHDGSIQDNDFEDMMTRYKEVNKGSLSDADYKSMQASLEDEWR 63

Query: 301 GL-SXAXXDXXGQXSKEEWISLWEKFXXXPS------XWXNLXXKFIFQXEDXSNXGXIX 459
            L   A  +     S EE++++WEK             W      F+F+  D S  G + 
Sbjct: 64  DLKGRADINKDDVVSWEEYLAMWEKTIATCKSVADLPAWCQNRIPFLFKGMDVSGDGIVD 123

Query: 460 XEEFSXVXAXFGL 498
            EEF      F L
Sbjct: 124 LEEFQNYCKNFQL 136


>UniRef50_Q4C9W1 Cluster: Calcium-binding EF-hand; n=1; Crocosphaera
           watsonii WH 8501|Rep: Calcium-binding EF-hand -
           Crocosphaera watsonii
          Length = 182

 Score = 48.0 bits (109), Expect = 3e-04
 Identities = 39/179 (21%), Positives = 70/179 (39%), Gaps = 4/179 (2%)
 Frame = +1

Query: 112 MVXDFRKXKLLHVXXXXFXXNGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTLLK 291
           M+   R+ KL H+       N  G++ ++DFE  IE I+  R W  G ++Y+E+    + 
Sbjct: 1   MLNPIRERKLSHLFHI-LDRNHDGVLSRQDFEQVIEEITNIRQWKWGTSEYEELHFFWMG 59

Query: 292 VWDGLS-XAXXDXXGQXSKEEWISLWEKF--XXXPSXWXNLXXKFIFQXEDXSNXGXIXX 462
             + L   A  +  G+ ++ EW+   E+       S           +  D S    +  
Sbjct: 60  FCNRLEVWADRNGDGKVTESEWLWYLEQMLDRFSASYIQQAFINISLKVMDFSRDDRVSL 119

Query: 463 EEFSXVXAXFGLXKXEXXXXFXKXXKGK-XXVXXXEXXELXKEFXXXEDVXXPGXFVXG 636
           +EF      + +   E    F      +   +   E   L +EF   E+   PG ++ G
Sbjct: 120 DEFKQFYQIYEIDPQEAAQAFVHLDLNQDGYLTKDELTSLFQEFFYSENPQSPGNWLWG 178


>UniRef50_P45961 Cluster: Putative calcium-binding protein cex-2;
           n=5; Caenorhabditis|Rep: Putative calcium-binding
           protein cex-2 - Caenorhabditis elegans
          Length = 166

 Score = 37.5 bits (83), Expect = 0.48
 Identities = 31/139 (22%), Positives = 58/139 (41%), Gaps = 4/139 (2%)
 Frame = +1

Query: 121 DFRKXKLLHVXXXXFXXNGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTLLKVWD 300
           +F + K  H     F  +  G+I+ KDF+  IE I + RG  +    +   +  L  +W 
Sbjct: 10  EFLESKWKHAFTTFFDLDQNGLIEWKDFKDLIEVIGEVRGRRSD--FFMTARLCLPDIWQ 67

Query: 301 GLSXA-XXDXXGQXSKEEWISLWE---KFXXXPSXWXNLXXKFIFQXEDXSNXGXIXXEE 468
            ++ A   +     +  +WI L +   K    P+ W     +++F+  D S    +   E
Sbjct: 68  KMTEAIGKEEEDIITLSDWIQLCQSSRKSVREPA-WQKAYVEYMFKLLDESGDHLVDQAE 126

Query: 469 FSXVXAXFGLXKXEXXXXF 525
           +  V   FG+ + +    F
Sbjct: 127 YVQVLGYFGVNRKDSSHCF 145


>UniRef50_UPI0000DB6F98 Cluster: PREDICTED: hypothetical protein;
           n=1; Apis mellifera|Rep: PREDICTED: hypothetical protein
           - Apis mellifera
          Length = 343

 Score = 35.1 bits (77), Expect = 2.5
 Identities = 22/90 (24%), Positives = 38/90 (42%), Gaps = 2/90 (2%)
 Frame = +1

Query: 109 KMVXDFRKXKLLHVXXXXFXXNGXGIIDKKDFELAIERISKSRGWSAGDAQYKEVQXTLL 288
           K +   ++ K  H        +  G I++KDF +  ER+ +   WS    +Y      LL
Sbjct: 112 KDLAPIQRDKFSHFFTYLLDYDRDGFINRKDFRMLSERLRRFADWSWNGPEY----LRLL 167

Query: 289 KVWDGLSXAXXD--XXGQXSKEEWISLWEK 372
           ++  GL+          + S EEW+  W +
Sbjct: 168 EIEQGLADLIFQEKKHDRISLEEWLCWWAR 197


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 372,814,869
Number of Sequences: 1657284
Number of extensions: 4074878
Number of successful extensions: 6818
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 6756
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6810
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 84441173866
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)