BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_F06 (1462 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 38 0.005 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.099 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.70 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 1.2 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 2.1 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.9 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 4.9 SPBC902.04 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 27 6.5 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 8.6 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 37.5 bits (83), Expect = 0.005 Identities = 25/98 (25%), Positives = 27/98 (27%) Frame = +3 Query: 807 PPXXAXXXPPQQXPPXXHAXKDHDRXTXXPTPHXHRPTAGRPPAPDTHQXATXXPXQTPX 986 PP PP H +H P P H P PP P H+ P P Sbjct: 236 PPMHHEPGEHMPPPPMHHEPGEH----MPPPPMHHEPGEHMPPPPMHHEPGEHMP---PP 288 Query: 987 KTXHAXXXPXPPXPHXTTPXRQRSSXPRXRXPXXHAXP 1100 H PP P P P P H P Sbjct: 289 PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPP 326 Score = 34.7 bits (76), Expect = 0.033 Identities = 19/69 (27%), Positives = 21/69 (30%) Frame = +3 Query: 894 PTPHXHRPTAGRPPAPDTHQXATXXPXQTPXKTXHAXXXPXPPXPHXTTPXRQRSSXPRX 1073 P P H+P PP P H+ P P H PP P P P Sbjct: 209 PPPMHHKPGEHMPPPPMHHEPGEHMP---PPPMHHEPGEHMPPPPMHHEPGEHMPPPPMH 265 Query: 1074 RXPXXHAXP 1100 P H P Sbjct: 266 HEPGEHMPP 274 Score = 34.3 bits (75), Expect = 0.043 Identities = 25/98 (25%), Positives = 27/98 (27%) Frame = +3 Query: 807 PPXXAXXXPPQQXPPXXHAXKDHDRXTXXPTPHXHRPTAGRPPAPDTHQXATXXPXQTPX 986 PP PP H +H P P H P PP P H+ P P Sbjct: 210 PPMHHKPGEHMPPPPMHHEPGEH----MPPPPMHHEPGEHMPPPPMHHEPGEHMPPP-PM 264 Query: 987 KTXHAXXXPXPPXPHXTTPXRQRSSXPRXRXPXXHAXP 1100 P PP H P P P H P Sbjct: 265 HHEPGEHMPPPPMHH--EPGEHMPPPPMHHEPGEHMPP 300 Score = 30.7 bits (66), Expect = 0.53 Identities = 20/75 (26%), Positives = 22/75 (29%) Frame = +3 Query: 807 PPXXAXXXPPQQXPPXXHAXKDHDRXTXXPTPHXHRPTAGRPPAPDTHQXATXXPXQTPX 986 PP PP H +H P P H P PP P H+ P P Sbjct: 275 PPMHHEPGEHMPPPPMHHEPGEH----MPPPPMHHEPGEHMPPPPMHHEPGEHMPPP-PM 329 Query: 987 KTXHAXXXPXPPXPH 1031 P PP H Sbjct: 330 HHEPGEHMPPPPFKH 344 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 33.1 bits (72), Expect = 0.099 Identities = 29/139 (20%), Positives = 41/139 (29%), Gaps = 4/139 (2%) Frame = +3 Query: 714 PXTRAXPPPXPXTQXXXXXXXXXXXXETXXRPPXXAXXXPPQQXPPXX--HAXKDHDRXT 887 P PPP T P + PP P A ++ Sbjct: 251 PAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKR 310 Query: 888 XXPTPHXHRPTAGRPPAPDTHQXATXXPXQTPXKTXHAXXXPXPPXPHXTTPXRQRSSXP 1067 P P R G+PP + ++ P P ++ A P PP P S P Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370 Query: 1068 R--XRXPXXHAXPXXTNPP 1118 + P + +NPP Sbjct: 371 STGRQPPPLSSSRAVSNPP 389 Score = 30.3 bits (65), Expect = 0.70 Identities = 18/64 (28%), Positives = 20/64 (31%) Frame = +1 Query: 145 PRPXXKGAGDAGTAPPQRXKAHPNXXXNXPPXXPPXPXTXXQPAAXPXGAGPXPPXGXXX 324 P P + PP + P PP P P PA P A P PP Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMP-AAPPLPPAAPAP 476 Query: 325 XPGP 336 P P Sbjct: 477 PPAP 480 Score = 29.1 bits (62), Expect = 1.6 Identities = 22/100 (22%), Positives = 27/100 (27%) Frame = +3 Query: 723 RAXPPPXPXTQXXXXXXXXXXXXETXXRPPXXAXXXPPQQXPPXXHAXKDHDRXTXXPTP 902 R PPP P + + PP PP+ R P P Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPP----PPRSNAAGSIPLPPQGRSAPPPPP 365 Query: 903 HXHRPTAGRPPAPDTHQXATXXPXQTPXKTXHAXXXPXPP 1022 P+ GR P P + A P P PP Sbjct: 366 PRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPP 405 Score = 27.9 bits (59), Expect = 3.7 Identities = 18/54 (33%), Positives = 21/54 (38%), Gaps = 1/54 (1%) Frame = +1 Query: 181 TAPPQRXKAHPNXXXNXPPXXPPXPXTXXQPAAXPXGAG-PXPPXGXXXXPGPP 339 +APP + P P PP P + P A P AG P P P PP Sbjct: 426 SAPPSLPPSAPPSLPMGAPAAPPLPPS--APIAPPLPAGMPAAPPLPPAAPAPP 477 Score = 27.5 bits (58), Expect = 4.9 Identities = 24/90 (26%), Positives = 31/90 (34%), Gaps = 4/90 (4%) Frame = +1 Query: 187 PPQRXKAHP---NXXXNXPPXXPPXPXTXXQPAAXPXGAGPXPPXG-XXXXPGPPXDTPX 354 P +R + P N N PP P P + G+ P PP G P PP P Sbjct: 317 PSRRNRGKPPIGNGSSNSSLPPPPPP-----PRSNAAGSIPLPPQGRSAPPPPPPRSAPS 371 Query: 355 TXXXAXQXPATAGXPHSTTPXGPXXXXPXK 444 T Q P + + P P P + Sbjct: 372 T---GRQPPPLSSSRAVSNPPAPPPAIPGR 398 Score = 27.5 bits (58), Expect = 4.9 Identities = 18/69 (26%), Positives = 19/69 (27%) Frame = +1 Query: 232 PPXXPPXPXTXXQPAAXPXGAGPXPPXGXXXXPGPPXDTPXTXXXAXQXPATAGXPHSTT 411 PP P P + P A P P G P P P PA P Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLP-PAA 473 Query: 412 PXGPXXXXP 438 P P P Sbjct: 474 PAPPPAPAP 482 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 30.3 bits (65), Expect = 0.70 Identities = 23/96 (23%), Positives = 26/96 (27%), Gaps = 6/96 (6%) Frame = +1 Query: 151 PXXKGAGDAGTAPPQRXKAHPNXXXNXPPXX------PPXPXTXXQPAAXPXGAGPXPPX 312 P K + AP R P PP PP P P + P P Sbjct: 995 PLSKPVSTSPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPK 1054 Query: 313 GXXXXPGPPXDTPXTXXXAXQXPATAGXPHSTTPXG 420 P P P PA +G P P G Sbjct: 1055 SSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSG 1090 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 29.5 bits (63), Expect = 1.2 Identities = 14/36 (38%), Positives = 14/36 (38%) Frame = -3 Query: 338 GGPGXXXXPXGGXGPAPXGXAAGWXXVSGXGGXCGG 231 GGPG GG G P G G G G GG Sbjct: 228 GGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGG 263 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.7 bits (61), Expect = 2.1 Identities = 23/93 (24%), Positives = 27/93 (29%), Gaps = 7/93 (7%) Frame = +1 Query: 166 AGDAGTAPPQRXKAHPNXXXNXP-----PXXPPXPXTXXQPAAXPXGAGPXPPXGXXXXP 330 A + APP P N P P P + PA P P P Sbjct: 1471 APSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQPPSSVAPATAPSSTLPPSQSSFAHVP 1530 Query: 331 GPPXDTPXTXXXAX--QXPATAGXPHSTTPXGP 423 P P A PA PH ++P P Sbjct: 1531 SPAPPAPQHPSAAALSSAPADNSMPHRSSPYAP 1563 Score = 27.1 bits (57), Expect = 6.5 Identities = 16/59 (27%), Positives = 21/59 (35%), Gaps = 2/59 (3%) Frame = +1 Query: 181 TAPPQRXKAHPNXXXNXPPXXPPXPXTXXQPAAXPXGAGP--XPPXGXXXXPGPPXDTP 351 + PP R ++ + P PP P+A P AGP PP P P Sbjct: 1688 STPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.5 bits (58), Expect = 4.9 Identities = 21/69 (30%), Positives = 23/69 (33%) Frame = +1 Query: 145 PRPXXKGAGDAGTAPPQRXKAHPNXXXNXPPXXPPXPXTXXQPAAXPXGAGPXPPXGXXX 324 PRP A +APP KA P PP P P P + P PP Sbjct: 145 PRPSIPPPSPA-SAPPIPSKAPPIPSSLPPPAQPAAP--VKSPPSAPSLPSAVPPMPPKV 201 Query: 325 XPGPPXDTP 351 P P P Sbjct: 202 PPPPLSQAP 210 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 27.5 bits (58), Expect = 4.9 Identities = 13/38 (34%), Positives = 13/38 (34%) Frame = +3 Query: 237 TXPPXPGXXXPAXXXXXGXRPPPPXXXXRXTGPPXXHP 350 T P P P G PPPP GPP P Sbjct: 743 TPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780 Score = 27.1 bits (57), Expect = 6.5 Identities = 13/39 (33%), Positives = 13/39 (33%) Frame = +1 Query: 235 PXXPPXPXTXXQPAAXPXGAGPXPPXGXXXXPGPPXDTP 351 P P P PA G P PP GPP P Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780 >SPBC902.04 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 589 Score = 27.1 bits (57), Expect = 6.5 Identities = 16/45 (35%), Positives = 18/45 (40%) Frame = +1 Query: 175 AGTAPPQRXKAHPNXXXNXPPXXPPXPXTXXQPAAXPXGAGPXPP 309 A TAP + + P P PP P PAA P P PP Sbjct: 146 AATAPSEFMPSIPGFGNLPNPAMPPIPFLPFNPAAQP----PFPP 186 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 26.6 bits (56), Expect = 8.6 Identities = 13/54 (24%), Positives = 18/54 (33%) Frame = +2 Query: 149 APSXKXRATPAPHRPSAXKXTPTXXPTPXHXPPXXRXPXXSXXXXXXXPAPXPP 310 AP+ + + + P+ P P PP P S P P PP Sbjct: 142 APASQEPSASGVNAPTVSAPNSMVSPPPSFQPPSAAAPATSLPSDYNPPPPPPP 195 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.305 0.128 0.414 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,448,202 Number of Sequences: 5004 Number of extensions: 30267 Number of successful extensions: 92 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 69 length of database: 2,362,478 effective HSP length: 75 effective length of database: 1,987,178 effective search space used: 816730158 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 43 (21.9 bits)
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