Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_E13
         (850 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha...   141   1e-34
SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar...   140   2e-34
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce...    26   5.9  
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc...    26   7.8  

>SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  141 bits (342), Expect = 1e-34
 Identities = 76/153 (49%), Positives = 95/153 (62%)
 Frame = +3

Query: 69  LGLRRAXWXQRXAXGGSXLRPSAXQRXSAXGRPAANTXLGPXRXHSVRSRGGNXKXRALR 248
           +G+ R    +R A G    +    +R    GR  +NT +GP R H VR RGGN K RALR
Sbjct: 1   MGITRDSRHKRSATGAKRAQ-YRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALR 59

Query: 249 LDTGNFSWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTL 428
           LD+GNFSWGSE  ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY +
Sbjct: 60  LDSGNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGI 119

Query: 429 PLGRKKGAKLTEAEEAIINKKRSQKTARKYLAR 527
            +G  KG K T          +S+   RK+ AR
Sbjct: 120 LMG-SKGKKATSTP-----NPKSKHVQRKHSAR 146



 Score = 66.5 bits (155), Expect = 4e-12
 Identities = 31/50 (62%), Positives = 36/50 (72%)
 Frame = +2

Query: 542 KVEGALXEQFHTGRLLACVASRPGQCGRADGYILXGXELEFYLRKIKSKR 691
           KV+ AL  QF  GRL A V+SRPGQ GR DGYIL G EL FYLR++  K+
Sbjct: 151 KVDSALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200


>SPAC521.05 |rps802|rps8-2|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  140 bits (340), Expect = 2e-34
 Identities = 76/153 (49%), Positives = 95/153 (62%)
 Frame = +3

Query: 69  LGLRRAXWXQRXAXGGSXLRPSAXQRXSAXGRPAANTXLGPXRXHSVRSRGGNXKXRALR 248
           +G+ R    +R A G    +    +R    GR  +NT +GP R H VR RGGN K RALR
Sbjct: 1   MGITRDSRHKRSATGAKRAQ-YRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALR 59

Query: 249 LDTGNFSWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTL 428
           LD+GNFSWGSE  ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY +
Sbjct: 60  LDSGNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGI 119

Query: 429 PLGRKKGAKLTEAEEAIINKKRSQKTARKYLAR 527
            +G  KG K T          +S+   RK+ AR
Sbjct: 120 LMG-SKGKKATATP-----TPKSKHVQRKHSAR 146



 Score = 66.5 bits (155), Expect = 4e-12
 Identities = 31/50 (62%), Positives = 36/50 (72%)
 Frame = +2

Query: 542 KVEGALXEQFHTGRLLACVASRPGQCGRADGYILXGXELEFYLRKIKSKR 691
           KV+ AL  QF  GRL A V+SRPGQ GR DGYIL G EL FYLR++  K+
Sbjct: 151 KVDSALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200


>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 817

 Score = 26.2 bits (55), Expect = 5.9
 Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
 Frame = +3

Query: 270 WGSEC--STRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRK 443
           WGS+     +K  + +V+  +++ +  +T  L         AT F  + +S+Y +PL R+
Sbjct: 250 WGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDRE 309

Query: 444 K 446
           +
Sbjct: 310 Q 310


>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
           Prp43|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 735

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = -1

Query: 409 YHCLNGVASTTTIAFLTRVFVRTNS 335
           ++C N V S T +  +  VFVR NS
Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,280,544
Number of Sequences: 5004
Number of extensions: 35804
Number of successful extensions: 97
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)