Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_E12
(868 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 148 1e-36
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 139 4e-34
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 83 5e-17
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 76 8e-15
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 148 bits (358), Expect = 1e-36
Identities = 63/90 (70%), Positives = 73/90 (81%)
Frame = +2
Query: 143 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 322
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 323 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPE 412
+VPR+++VDLEP V+D+VRTG YR LFHPE
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPE 90
Score = 116 bits (278), Expect = 6e-27
Identities = 65/137 (47%), Positives = 82/137 (59%), Gaps = 2/137 (1%)
Frame = +3
Query: 402 FIQNQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 581
F QLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 87 FHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTG 146
Query: 582 XXXXXLLMERLSG*L--RQEV*TGVRHLXRASGFHXRRRALQXYPHHPHNLEHSDCAFMV 755
LL+ERL+ + ++ V + S H L+ +DC FMV
Sbjct: 147 SGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTH--ATLDLADCTFMV 204
Query: 756 DXEAIYDIXRRNLXIER 806
D E+ YDI RRNL IER
Sbjct: 205 DNESCYDICRRNLDIER 221
Score = 46.0 bits (104), Expect = 7e-06
Identities = 19/27 (70%), Positives = 24/27 (88%)
Frame = +1
Query: 619 VDYGKKSKLEFAIXPAPQVSTXVVEPY 699
++Y KKSKL+F++ PAPQVST VVEPY
Sbjct: 159 MEYTKKSKLQFSVYPAPQVSTSVVEPY 185
Score = 33.1 bits (72), Expect = 0.053
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = +2
Query: 806 PTYTNLNRPIGQIVSSITXSL 868
P+Y NLNR I Q+VSSIT SL
Sbjct: 222 PSYENLNRLIAQVVSSITASL 242
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 139 bits (337), Expect = 4e-34
Identities = 66/95 (69%), Positives = 74/95 (77%), Gaps = 5/95 (5%)
Frame = +2
Query: 143 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 307
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 308 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPE 412
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPE
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPE 94
Score = 120 bits (288), Expect = 4e-28
Identities = 67/140 (47%), Positives = 85/140 (60%), Gaps = 2/140 (1%)
Frame = +3
Query: 402 FIQNQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 581
F Q++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 91 FHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTG 150
Query: 582 XXXXXLLMERLSG*L--RQEV*TGVRHLXRASGFHXRRRALQXYPHHPHNLEHSDCAFMV 755
LL+ERL+ + + V + S H L++SDC FMV
Sbjct: 151 SGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTH--ATLDNSDCTFMV 208
Query: 756 DXEAIYDIXRRNLXIERQPT 815
D EA YDI RRNL IER PT
Sbjct: 209 DNEACYDICRRNLDIER-PT 227
Score = 47.2 bits (107), Expect = 3e-06
Identities = 19/27 (70%), Positives = 24/27 (88%)
Frame = +1
Query: 619 VDYGKKSKLEFAIXPAPQVSTXVVEPY 699
++YGKKS L+F++ PAPQVST VVEPY
Sbjct: 163 MEYGKKSNLQFSVYPAPQVSTSVVEPY 189
Score = 34.7 bits (76), Expect = 0.017
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = +2
Query: 806 PTYTNLNRPIGQIVSSITXSL 868
PTY NLNR I Q+VSSIT SL
Sbjct: 226 PTYENLNRLIAQVVSSITASL 246
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 83.0 bits (196), Expect = 5e-17
Identities = 38/89 (42%), Positives = 57/89 (64%)
Frame = +2
Query: 146 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 325
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 326 VPRAVFVDLEPTVVDEVRTGTYRQLFHPE 412
+PRA+ +DLEP VV+ + + TY L++PE
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPE 89
Score = 35.5 bits (78), Expect = 0.010
Identities = 23/71 (32%), Positives = 35/71 (49%), Gaps = 1/71 (1%)
Frame = +3
Query: 408 QNQLITGKEDAA-NNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXX 584
+N LIT A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 89 ENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGS 147
Query: 585 XXXXLLMERLS 617
L+ERL+
Sbjct: 148 GLGSFLLERLN 158
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 75.8 bits (178), Expect = 8e-15
Identities = 37/94 (39%), Positives = 50/94 (53%)
Frame = +2
Query: 143 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 322
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 323 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEPTYY 424
+VPRAV VDLEP +D V++G + LF P+ Y
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIY 92
Score = 55.6 bits (128), Expect = 9e-09
Identities = 26/71 (36%), Positives = 39/71 (54%)
Frame = +3
Query: 402 FIQNQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 581
F + +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144
Query: 582 XXXXXLLMERL 614
LL+ ++
Sbjct: 145 SGMGTLLLSKI 155
Score = 27.9 bits (59), Expect = 2.0
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1
Query: 622 DYGKKSKLEFAIXPAPQVSTXVVEPY 699
+Y + F++ PAP+ S VVEPY
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPY 183
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.0
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 222 STASSLMARCPQTRPSGVETILSTLSSARPELAST 326
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 241 WPDAHRQDHRGWRRFFQHFLQR 306
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,362,760
Number of Sequences: 5004
Number of extensions: 66716
Number of successful extensions: 178
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 175
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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