Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_E02
(887 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 102 6e-23
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 100 6e-22
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 72 1e-13
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 69 7e-13
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 62 8e-11
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 53 6e-08
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 42 1e-04
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 1.5
SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 27 2.7
SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce... 27 2.7
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.6
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 27 4.7
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 27 4.7
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 26 6.2
SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 26 6.2
SPAC9E9.17c |||dubiuos|Schizosaccharomyces pombe|chr 1|||Manual 26 8.2
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 102 bits (245), Expect = 6e-23
Identities = 53/134 (39%), Positives = 73/134 (54%), Gaps = 8/134 (5%)
Frame = +1
Query: 394 ARTWC------AVP--CRRPWNNLNFQYLDLYLIHWPQAYKEDGSLFPTDQSGKIQFSDV 549
++ WC AVP + +L YLD YLIHWP ++K FP D+ G + +
Sbjct: 84 SKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKFPKDKDGNLIYEKN 143
Query: 550 DYVDTWKAMEPLVKEGLVKSIGVSNFNSKQLERLLXHAXVKPVVNXVECHPYLNXKRLKE 729
+TWKAME L++ G V+ IG+SNFN LER+L A VKP V+ +E HP+L E
Sbjct: 144 PIEETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAKVKPAVHQMELHPFLPQTEFVE 203
Query: 730 FCXXRGVXIXXYSP 771
G+ + YSP
Sbjct: 204 KHKKLGIHVTAYSP 217
Score = 97.5 bits (232), Expect = 2e-21
Identities = 46/92 (50%), Positives = 61/92 (66%)
Frame = +3
Query: 180 PXFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTREDL 359
P GLGTW+S+P + AVK A+ GYRHID A +YGNE EVG+ IKE V R+D+
Sbjct: 25 PGLGLGTWRSEPNQTKNAVKTALQYGYRHIDAAAIYGNEDEVGDG----IKESGVPRKDI 80
Query: 360 FITSKLWNTFHRPDLVRGALQETLEQSELSVL 455
++TSKLW H P+ V AL++TL+ +L L
Sbjct: 81 WVTSKLWCNAHAPEAVPKALEKTLKDLKLDYL 112
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 99.5 bits (237), Expect = 6e-22
Identities = 45/112 (40%), Positives = 68/112 (60%)
Frame = +1
Query: 436 NLNFQYLDLYLIHWPQAYKEDGSLFPTDQSGKIQFSDVDYVDTWKAMEPLVKEGLVKSIG 615
+LN +Y+D YLIHWP A P ++ G++ + DV +TW+AME L++ G V+ IG
Sbjct: 105 DLNLEYIDAYLIHWPFALLSGPEELPRNEKGELIYEDVPIEETWQAMEELLETGKVRYIG 164
Query: 616 VSNFNSKQLERLLXHAXVKPVVNXVECHPYLNXKRLKEFCXXRGVXIXXYSP 771
+SNFN++ L+R+L A VKP ++ +E HPYL E + + YSP
Sbjct: 165 ISNFNNEYLDRVLKIAKVKPTIHQMELHPYLPQTEYLEKHKKLQIHVSAYSP 216
Score = 90.6 bits (215), Expect = 3e-19
Identities = 45/92 (48%), Positives = 58/92 (63%)
Frame = +3
Query: 180 PXFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTREDL 359
P GLGTW+S E AV A+ GYRHID A++YGNEKE+GE I+E V R D+
Sbjct: 24 PSIGLGTWRSGKDETKNAVCAALKAGYRHIDTAHIYGNEKEIGEG----IRESGVPRTDI 79
Query: 360 FITSKLWNTFHRPDLVRGALQETLEQSELSVL 455
++TSKLW HR LV AL++TL+ L +
Sbjct: 80 WVTSKLWCNAHRAGLVPLALEKTLQDLNLEYI 111
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 72.1 bits (169), Expect = 1e-13
Identities = 46/113 (40%), Positives = 57/113 (50%), Gaps = 2/113 (1%)
Frame = +1
Query: 439 LNFQYLDLYLIHWPQAYKEDGSLFPTDQSGKIQFSDVDYVDTWKAMEPLVKEGLVKSIGV 618
L YLDLYL+H P + E KI S+ WKAME + GLV S+GV
Sbjct: 98 LGTDYLDLYLLHSPIPFYEK----------KIPISE-----GWKAMETALGTGLVHSVGV 142
Query: 619 SNFNSKQLERLLXHAXVKPVVNXVECHP--YLNXKRLKEFCXXRGVXIXXYSP 771
SNF LE LL + + P VN +E HP Y K L EFC +G+ + Y P
Sbjct: 143 SNFRIPDLEELLKTSTITPRVNQIEFHPQVYKAAKPLVEFCQSKGIIVEGYGP 195
Score = 65.7 bits (153), Expect = 8e-12
Identities = 41/92 (44%), Positives = 57/92 (61%), Gaps = 6/92 (6%)
Frame = +3
Query: 180 PXFGLGT--WKSKPGEVTQ----AVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGV 341
P +G+GT +K + GE+ + +VK+A+ G+ HIDCA VYGNE+EVG A +KE
Sbjct: 13 PAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAEVYGNEEEVGVA----LKEAN 68
Query: 342 VTREDLFITSKLWNTFHRPDLVRGALQETLEQ 437
V R LFITSK+ H D + AL E+L +
Sbjct: 69 VPRSKLFITSKV---MHNVDNIPEALNESLRK 97
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 69.3 bits (162), Expect = 7e-13
Identities = 27/75 (36%), Positives = 50/75 (66%), Gaps = 1/75 (1%)
Frame = +1
Query: 550 DYVDTWKAMEPLVKEGLVKSIGVSNFNSKQLERLL-XHAXVKPVVNXVECHPYLNXKRLK 726
D +++WKA+E V+EG +++IGVSNF ++ LL H + P VN +E HP+ + +++
Sbjct: 116 DRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVV 175
Query: 727 EFCXXRGVXIXXYSP 771
++C +G+ + Y+P
Sbjct: 176 DYCESKGIQLAAYAP 190
Score = 52.4 bits (120), Expect = 8e-08
Identities = 26/66 (39%), Positives = 37/66 (56%)
Frame = +3
Query: 180 PXFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTREDL 359
P F G++ + +V A+ GYRHID A +Y NE + G AI ++E RED+
Sbjct: 15 PQFAYGSYMVNRTKCFDSVYAALQCGYRHIDSAQMYHNEADCGRAILKFMEETGTKREDI 74
Query: 360 FITSKL 377
+ TSKL
Sbjct: 75 WFTSKL 80
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 62.5 bits (145), Expect = 8e-11
Identities = 32/70 (45%), Positives = 40/70 (57%)
Frame = +3
Query: 174 LFPXFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGVVTRE 353
+ P G G + K E V A+D GYRHID A VYGNE G+AI ++ V R
Sbjct: 18 VIPRIGFGAFMLKYNECYGLVTQALDSGYRHIDTAAVYGNEDICGKAIVDWCEKNNVKRT 77
Query: 354 DLFITSKLWN 383
D+F+TSKL N
Sbjct: 78 DIFLTSKLAN 87
Score = 60.5 bits (140), Expect = 3e-10
Identities = 27/73 (36%), Positives = 42/73 (57%), Gaps = 1/73 (1%)
Frame = +1
Query: 556 VDTWKAMEPLVKEGLVKSIGVSNFNSKQLERL-LXHAXVKPVVNXVECHPYLNXKRLKEF 732
+ +WKAME V G ++S+GVSN+ K L+ L + P VN +E HP+L+ + ++
Sbjct: 125 IASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIELHPFLSQDDIVKY 184
Query: 733 CXXRGVXIXXYSP 771
C + I YSP
Sbjct: 185 CQSHDIAIEAYSP 197
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 52.8 bits (121), Expect = 6e-08
Identities = 22/72 (30%), Positives = 37/72 (51%)
Frame = +1
Query: 556 VDTWKAMEPLVKEGLVKSIGVSNFNSKQLERLLXHAXVKPVVNXVECHPYLNXKRLKEFC 735
+++W+A+ KEG + IGVSN+N LE ++ P +N VE + N +C
Sbjct: 119 IESWRALLQHQKEGRINKIGVSNYNIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYC 178
Query: 736 XXRGVXIXXYSP 771
G+ + +SP
Sbjct: 179 FNHGILVQAFSP 190
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 41.9 bits (94), Expect = 1e-04
Identities = 18/41 (43%), Positives = 26/41 (63%)
Frame = +1
Query: 553 YVDTWKAMEPLVKEGLVKSIGVSNFNSKQLERLLXHAXVKP 675
+VDTWK +E V EG + ++GVS F +L+RL+ V P
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVP 198
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/47 (44%), Positives = 28/47 (59%)
Frame = +2
Query: 500 ALSSRPTNQVKSSSRTSITWTPGRLWSRSSKRVWSRASESPTSTRSS 640
A SS T+ V SSS+ S + + L S SS + S +S SPTS+ SS
Sbjct: 75 ASSSFITSLVSSSSQQSSS-SSASLTSSSSATLTSSSSASPTSSSSS 120
>SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr
3|||Manual
Length = 872
Score = 27.5 bits (58), Expect = 2.7
Identities = 7/23 (30%), Positives = 17/23 (73%)
Frame = +1
Query: 523 SGKIQFSDVDYVDTWKAMEPLVK 591
S +++F+ +DY+ W+A+ P+ +
Sbjct: 162 SQRVEFARLDYIKVWRALAPIYR 184
>SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1261
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 390 HRPDLVRGALQETLEQSELSVLGSVPDTLAPGLQGRRLSLPDRPI 524
HRP L G + ++Q + S+ +T APG RL L + I
Sbjct: 24 HRPPLKLGNMTNWIQQQQAQTNLSLSNTKAPGSLDERLFLTEDDI 68
>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 317
Score = 27.1 bits (57), Expect = 3.6
Identities = 26/88 (29%), Positives = 42/88 (47%)
Frame = +2
Query: 470 YTGPRPTRKTALSSRPTNQVKSSSRTSITWTPGRLWSRSSKRVWSRASESPTSTRSSWRG 649
Y+G + A S T S TS + TP S SS S +S+S +S++SS
Sbjct: 123 YSGTISSTSIAPSMIGTRTSSSYFITSSSSTPSSSSSSSSSSPSSSSSKSSSSSKSSSSS 182
Query: 650 CXXTRXSSRSXIXWNVILTSTXNASRSS 733
++ SS S + +S+ ++S+SS
Sbjct: 183 SSSSKSSSSSSSS-SKSSSSSSSSSKSS 209
Score = 27.1 bits (57), Expect = 3.6
Identities = 17/49 (34%), Positives = 31/49 (63%)
Frame = +2
Query: 494 KTALSSRPTNQVKSSSRTSITWTPGRLWSRSSKRVWSRASESPTSTRSS 640
K++ SS+ ++ SSS++S + + S SS S++S SP+S++SS
Sbjct: 171 KSSSSSKSSSSSSSSSKSSSSSSSSSKSSSSSSSS-SKSSASPSSSKSS 218
Score = 26.6 bits (56), Expect = 4.7
Identities = 24/76 (31%), Positives = 36/76 (47%)
Frame = +2
Query: 506 SSRPTNQVKSSSRTSITWTPGRLWSRSSKRVWSRASESPTSTRSSWRGCXXTRXSSRSXI 685
SS+ ++ KSSS +S + S+SS S + S +S+ SS + S S
Sbjct: 169 SSKSSSSSKSSSSSSSS-------SKSSSSSSSSSKSSSSSSSSSKSSASPSSSKSSSKF 221
Query: 686 XWNVILTSTXNASRSS 733
+ +TST AS SS
Sbjct: 222 SSSSFITSTTPASSSS 237
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 26.6 bits (56), Expect = 4.7
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 506 SSRPTNQVKSSSRTSITWTPGRLWSRSSK 592
SS+ ++ VK +S S TWTP L SK
Sbjct: 321 SSKHSSPVKGNSSISSTWTPSNLKIYHSK 349
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 26.6 bits (56), Expect = 4.7
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = -1
Query: 434 FQGLLQGTAHQVRAMKRVPQF--RSYEQIFPRYDSFLNFA 321
F ++G AH V +F +SY+ +FP D+F+ A
Sbjct: 424 FYNSVKGPAHSFETEDPVLKFVSQSYDDLFPYMDNFIQLA 463
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 26.2 bits (55), Expect = 6.2
Identities = 22/72 (30%), Positives = 31/72 (43%), Gaps = 7/72 (9%)
Frame = +3
Query: 186 FGLGTWKSKPGE------VTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSK-IKEGVV 344
F LG W + E +K A D+G D A +Y N E + K IKE
Sbjct: 28 FSLGGWLTYGNEGYDVEHTKNCLKQAWDLGINTFDTAEIYSNGN--SETVMGKAIKELGW 85
Query: 345 TREDLFITSKLW 380
R + IT+K++
Sbjct: 86 DRSEYVITTKVF 97
>SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 672
Score = 26.2 bits (55), Expect = 6.2
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +2
Query: 479 PRPTRKTALSSRPTNQVKSSSRTSITWTPGRLWSRSSKRVWSRASESPTSTRS 637
P P + T +S +PT + ++ R S++ SR+S R S ++ S S+ S
Sbjct: 528 PSPLQNTVISPQPTRRSRTPIR-SLSGRSSVASSRNSSRTRSYSNVSSASSNS 579
>SPAC9E9.17c |||dubiuos|Schizosaccharomyces pombe|chr 1|||Manual
Length = 72
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = -1
Query: 371 RSYEQIFPRYDSFLNFAGYSFSDFLLVSVNIGTVDVSVANVN 246
RS E + D LN +F+DF ++N+G + +S N
Sbjct: 29 RSREFSVIQKDEELNVLEINFNDFYRTTLNVGELSMSTRKTN 70
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,703,002
Number of Sequences: 5004
Number of extensions: 54068
Number of successful extensions: 211
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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