Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_D21
         (904 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC11E10.07c |||translation initiation factor eIF2B alpha subun...    68   2e-12
SPBC23E6.10c |||methylthioribose-1-phosphate isomerase |Schizosa...    53   6e-08
SPAC343.14c |||translation initiation factor eIF2B|Schizosacchar...    34   0.032
SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe...    27   4.8  
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    26   6.4  
SPAC23H3.05c |swd1||COMPASS complex subunit Swd1|Schizosaccharom...    26   8.4  

>SPCC11E10.07c |||translation initiation factor eIF2B alpha
           subunit|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 341

 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 32/73 (43%), Positives = 45/73 (61%)
 Frame = +2

Query: 641 SGEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIGTYSLGIXX 820
           SG +M   L  A +   ++L SAV + M  VD+V++GAEGV E+GG+IN IGT+ L +  
Sbjct: 174 SGCLMTRTLKNACIPTCMVLDSAVSFTMNRVDLVLVGAEGVVENGGLINQIGTFQLAVFA 233

Query: 821 LXLXKPXYVLXES 859
               KP Y + ES
Sbjct: 234 KHAHKPFYAVAES 246



 Score = 35.5 bits (78), Expect = 0.010
 Identities = 27/98 (27%), Positives = 49/98 (50%), Gaps = 3/98 (3%)
 Frame = +3

Query: 351 AISSGCELFMRVYYICQIRHK--EF*RM*KNYVTK-R*YISGTSC*KHEGKVAKQALSFI 521
           ++S+GC++F R  ++ +  H   +F +  ++ V   + +I      +   ++A      I
Sbjct: 78  SLSAGCDIFQR--FVTRSLHDVGDFEQCKRHLVENGKLFIQRARACRQ--RIAHLGYPLI 133

Query: 522 CDGCKIXXHSRSRVVLQAMIEAIKANKRFEVFITVSAP 635
            DG  I  H  SR V   ++ A K + RF+VF+T S P
Sbjct: 134 RDGSVILTHGFSRGVAAVLLAAAKRHVRFKVFVTESRP 171


>SPBC23E6.10c |||methylthioribose-1-phosphate isomerase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 359

 Score = 52.8 bits (121), Expect = 6e-08
 Identities = 28/84 (33%), Positives = 42/84 (50%), Gaps = 5/84 (5%)
 Frame = +2

Query: 611 SLHHSICT-----GHSGEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESG 775
           +L H+ CT          +   +LV   + ATL+  S V  +M  +D +++GA+ VT +G
Sbjct: 182 NLEHAYCTETRPYNQGSRLTAFELVHDKIPATLVTDSTVASIMHKIDAIVVGADRVTRNG 241

Query: 776 GIINXIGTYSLGIXXLXLXKPXYV 847
              N IGTY+L I      KP  V
Sbjct: 242 DTANKIGTYNLAILAKHFGKPFIV 265


>SPAC343.14c |||translation initiation factor
           eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 393

 Score = 33.9 bits (74), Expect = 0.032
 Identities = 14/48 (29%), Positives = 27/48 (56%)
 Frame = +2

Query: 653 MHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIG 796
           M  +L  AG+D T+I  + +  +M  V+ V++G   +  +GG++   G
Sbjct: 245 MAKRLAQAGIDTTVISDATIFAIMSRVNKVILGTHAILGNGGLVTYSG 292


>SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 727

 Score = 26.6 bits (56), Expect = 4.8
 Identities = 18/46 (39%), Positives = 23/46 (50%)
 Frame = -2

Query: 777 PPLSVTPSAPIITISTCSMT*PTAEXKISVASTPAATSCXCIISPL 640
           PPLSVTPS  I  +S      P +    S AS+ + T    I+ PL
Sbjct: 571 PPLSVTPSTAITNLSI-----PESNRTNSSASSKSFTMNDLILPPL 611


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 26.2 bits (55), Expect = 6.4
 Identities = 9/50 (18%), Positives = 26/50 (52%)
 Frame = +1

Query: 70  PWVRRVIVSILGSWAR*TTKNLNHEKKKESSKLIFHXSILEEEXKRDVSA 219
           P+++R+  +++  W+R   K   + + +      F+  + +   +RD++A
Sbjct: 300 PFIKRIADNLVSEWSRPIIKRSANYRDRAVGVASFNPEVFQTRRRRDLAA 349


>SPAC23H3.05c |swd1||COMPASS complex subunit
           Swd1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 398

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 15/31 (48%), Positives = 17/31 (54%)
 Frame = -1

Query: 352 AGTGLITMSHCIHSQLEIQI*FSHCRYFIIF 260
           A T  I  SH I SQ   QI  S C+ F+IF
Sbjct: 194 ASTTKIRSSHRITSQSIKQIRLSFCKRFLIF 224


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,222,729
Number of Sequences: 5004
Number of extensions: 62106
Number of successful extensions: 136
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)