Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_D21
(904 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53880.1 68414.m06133 eukaryotic translation initiation facto... 70 2e-12
At1g72340.1 68414.m08368 eukaryotic translation initiation facto... 69 3e-12
At1g53900.1 68414.m06136 eukaryotic translation initiation facto... 69 3e-12
At3g07300.1 68416.m00869 eukaryotic translation initiation facto... 45 8e-05
At2g05830.2 68415.m00631 eukaryotic translation initiation facto... 42 4e-04
At2g05830.1 68415.m00630 eukaryotic translation initiation facto... 42 4e-04
At5g38640.1 68418.m04673 eukaryotic translation initiation facto... 40 0.003
At2g44070.1 68415.m05481 eukaryotic translation initiation facto... 39 0.005
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 37 0.021
>At1g53880.1 68414.m06133 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|Q64270 Translation initiation factor eIF-2B alpha
subunit {Rattus norvegicus}; contains Pfam profiles
PF04525: Protein of unknown function (DUF567), PF01008:
Initiation factor 2 subunit family
Length = 637
Score = 69.7 bits (163), Expect = 2e-12
Identities = 34/82 (41%), Positives = 49/82 (59%)
Frame = +2
Query: 614 LHHSICTGHSGEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXI 793
L +CTG ++ +L + L++ SAV Y M+ VD+V +GA+GV ESGGIIN +
Sbjct: 489 LFRVLCTG---VLLANELAKLDIPVKLLIDSAVAYSMDEVDMVFVGADGVVESGGIINMM 545
Query: 794 GTYSLGIXXLXLXKPXYVLXES 859
GTY + + + KP YV ES
Sbjct: 546 GTYQIALVAQSMNKPVYVAAES 567
Score = 40.3 bits (90), Expect = 0.002
Identities = 30/93 (32%), Positives = 45/93 (48%), Gaps = 2/93 (2%)
Frame = +3
Query: 351 AISSGCELFMRVYYICQIRHKEF*--RM*KNYVTKR*YISGTSC*KHEGKVAKQALSFIC 524
++++GC+LFMR Y+ + EF K+ V +R G K +A + FI
Sbjct: 405 SLTAGCDLFMR--YVTRTSALEFEDFNSAKSRVLERAEKFGEISCKARTIIAMLSQDFIF 462
Query: 525 DGCKIXXHSRSRVVLQAMIEAIKANKRFEVFIT 623
DGC I H SRVV + + + + K F V T
Sbjct: 463 DGCTILVHGFSRVVFEILKTSAQNKKLFRVLCT 495
>At1g72340.1 68414.m08368 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|Q64270 Translation initiation factor eIF-2B alpha
subunit {Rattus norvegicus}; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 382
Score = 69.3 bits (162), Expect = 3e-12
Identities = 32/73 (43%), Positives = 46/73 (63%)
Frame = +2
Query: 641 SGEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIGTYSLGIXX 820
+G ++ +L + L+L SAV Y M+ VD+V +GA+GV ESGGIIN +GTY + +
Sbjct: 240 TGVLLSSELSKLDIPVKLLLDSAVAYSMDEVDMVFVGADGVVESGGIINMMGTYQIALVA 299
Query: 821 LXLXKPXYVLXES 859
+ KP YV ES
Sbjct: 300 HSMNKPVYVAAES 312
Score = 49.2 bits (112), Expect = 4e-06
Identities = 31/97 (31%), Positives = 48/97 (49%), Gaps = 1/97 (1%)
Frame = +3
Query: 351 AISSGCELFMR-VYYICQIRHKEF*RM*KNYVTKR*YISGTSC*KHEGKVAKQALSFICD 527
++++GC+LF+R V + +++F + + SC K +A + FI D
Sbjct: 143 SLTAGCDLFIRYVTRTSALEYEDFNSAKSRLLERAEKFGEISC-KARRIIAMLSQDFIFD 201
Query: 528 GCKIXXHSRSRVVLQAMIEAIKANKRFEVFITVSAPD 638
GC I H SRVVL+ + A + NK F V T PD
Sbjct: 202 GCTILVHGLSRVVLEILKTAAQNNKLFRVLCTEGRPD 238
>At1g53900.1 68414.m06136 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|Q64270 Translation initiation factor eIF-2B alpha
subunit {Rattus norvegicus}; contains Pfam profiles
PF04525: Protein of unknown function (DUF567), PF01008:
Initiation factor 2 subunit family
Length = 644
Score = 69.3 bits (162), Expect = 3e-12
Identities = 31/73 (42%), Positives = 46/73 (63%)
Frame = +2
Query: 641 SGEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIGTYSLGIXX 820
+G ++ +L + L++ SAV Y M+ VD+V +GA+GV ESGGIIN +GTY + +
Sbjct: 502 TGVLLANELAKLDIPVKLLIDSAVAYSMDEVDMVFVGADGVVESGGIINMMGTYQIALVA 561
Query: 821 LXLXKPXYVLXES 859
+ KP YV ES
Sbjct: 562 QSMNKPVYVAAES 574
Score = 44.4 bits (100), Expect = 1e-04
Identities = 32/98 (32%), Positives = 47/98 (47%), Gaps = 2/98 (2%)
Frame = +3
Query: 351 AISSGCELFMRVYYICQIRHKEF*--RM*KNYVTKR*YISGTSC*KHEGKVAKQALSFIC 524
++++GC+LFMR Y+ + EF K+ V +R G K +A + FI
Sbjct: 405 SLTAGCDLFMR--YVTRTSALEFEDFNSAKSRVLERAEKFGEISCKARTIIAMLSQDFIF 462
Query: 525 DGCKIXXHSRSRVVLQAMIEAIKANKRFEVFITVSAPD 638
DGC I H SRVV + + + + K F V T PD
Sbjct: 463 DGCTILVHGFSRVVFEILKTSAQNKKLFRVLCTEGRPD 500
>At3g07300.1 68416.m00869 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P49770 Translation initiation factor eIF-2B beta
subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF01008: Initiation factor 2
subunit family
Length = 407
Score = 44.8 bits (101), Expect = 8e-05
Identities = 24/74 (32%), Positives = 39/74 (52%)
Frame = +2
Query: 638 HSGEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIGTYSLGIX 817
+ G ++ +LVA G+ T+I SAV ++ V++V+IGA V +GG+I +G +
Sbjct: 246 YQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVGVNMAALA 305
Query: 818 XLXLXKPXYVLXES 859
P VL S
Sbjct: 306 AQKHAVPFVVLAGS 319
>At2g05830.2 68415.m00631 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
CIG2 [Nicotiana tabacum] GI:15216226, IDI2 [Hordeum
vulgare subsp. vulgare] GI:12407304; weak similarity to
SP|Q64270 Translation initiation factor eIF-2B alpha
subunit {Rattus norvegicus}; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 326
Score = 42.3 bits (95), Expect = 4e-04
Identities = 26/64 (40%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
Frame = +2
Query: 662 QLVAAGVDATLILXSAVGYVMEX--VDIVMIGAEGVTESGGIINXIGTYSLGIXXLXLXK 835
+LV + ATLI SA +M+ VD V++GA+ V +G N IGTYSL +
Sbjct: 172 ELVHEKIPATLIADSAAAALMKDGRVDGVIVGADRVASNGDTANKIGTYSLALCAKHHGI 231
Query: 836 PXYV 847
P YV
Sbjct: 232 PFYV 235
>At2g05830.1 68415.m00630 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
CIG2 [Nicotiana tabacum] GI:15216226, IDI2 [Hordeum
vulgare subsp. vulgare] GI:12407304; weak similarity to
SP|Q64270 Translation initiation factor eIF-2B alpha
subunit {Rattus norvegicus}; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 374
Score = 42.3 bits (95), Expect = 4e-04
Identities = 26/64 (40%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
Frame = +2
Query: 662 QLVAAGVDATLILXSAVGYVMEX--VDIVMIGAEGVTESGGIINXIGTYSLGIXXLXLXK 835
+LV + ATLI SA +M+ VD V++GA+ V +G N IGTYSL +
Sbjct: 220 ELVHEKIPATLIADSAAAALMKDGRVDGVIVGADRVASNGDTANKIGTYSLALCAKHHGI 279
Query: 836 PXYV 847
P YV
Sbjct: 280 PFYV 283
>At5g38640.1 68418.m04673 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P41111 Translation initiation factor eIF-2B delta
subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF01008: Initiation
factor 2 subunit family
Length = 642
Score = 39.5 bits (88), Expect = 0.003
Identities = 18/72 (25%), Positives = 36/72 (50%)
Frame = +2
Query: 644 GEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIGTYSLGIXXL 823
G+++ +L+ G++ T +A+ Y+M V V +GA V +G + + +GT + +
Sbjct: 483 GQLLLRRLIKRGINCTYTHINAISYIMHEVTKVFLGASSVLSNGTVYSRVGTACVAMVAN 542
Query: 824 XLXKPXYVLXES 859
P V E+
Sbjct: 543 AFRVPVLVCCEA 554
>At2g44070.1 68415.m05481 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|Q63186 Translation initiation factor eIF-2B delta
subunit (eIF-2B GDP-GTP exchange factor) {Rattus
norvegicus}; contains Pfam profile PF01008: Initiation
factor 2 subunit family
Length = 307
Score = 38.7 bits (86), Expect = 0.005
Identities = 18/72 (25%), Positives = 36/72 (50%)
Frame = +2
Query: 644 GEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIGTYSLGIXXL 823
G+++ +L+ G++ T +A+ Y+M V V +GA V +G + + +GT + +
Sbjct: 141 GKLLLRRLIKRGINCTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVAN 200
Query: 824 XLXKPXYVLXES 859
P V E+
Sbjct: 201 AFRVPVLVCCEA 212
Score = 29.1 bits (62), Expect = 4.2
Identities = 13/49 (26%), Positives = 26/49 (53%)
Frame = +3
Query: 495 VAKQALSFICDGCKIXXHSRSRVVLQAMIEAIKANKRFEVFITVSAPDI 641
+ + A++ I DG + + S V+ ++ A + K+F VF+ S P +
Sbjct: 91 IVEHAVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKL 139
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 36.7 bits (81), Expect = 0.021
Identities = 18/72 (25%), Positives = 33/72 (45%)
Frame = +2
Query: 644 GEMMHXQLVAAGVDATLILXSAVGYVMEXVDIVMIGAEGVTESGGIINXIGTYSLGIXXL 823
G+ + +LV G+D T +A+ Y+M V +GA + +G + +GT + +
Sbjct: 591 GQKLLRRLVTRGLDCTYTHINAISYIMREATRVFLGASSIYSNGTLYARVGTSCIAMVAN 650
Query: 824 XLXKPXYVLXES 859
P V E+
Sbjct: 651 AFSVPVIVCCEA 662
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,761,426
Number of Sequences: 28952
Number of extensions: 322326
Number of successful extensions: 729
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 726
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2129873112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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