Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_D12
(876 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 239 3e-64
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 134 2e-32
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 126 5e-30
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 105 1e-23
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 60 4e-10
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 58 2e-09
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 57 4e-09
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 43 7e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 36 0.008
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 239 bits (586), Expect = 3e-64
Identities = 112/139 (80%), Positives = 119/139 (85%)
Frame = +3
Query: 243 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 422
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 423 VLLTEAPLNPKGQQGRR*PRSCLRPSTPPPMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 602
LLTEAPLNPK + + + P YVAIQAVLSLYASGRTTGIVLDSGDGV+H
Sbjct: 103 CLLTEAPLNPKSNREKM-TQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTH 161
Query: 603 TVPIYEGYALPHAILRSGL 659
TVPIYEGYALPHAI+R L
Sbjct: 162 TVPIYEGYALPHAIMRLDL 180
Score = 110 bits (265), Expect = 2e-25
Identities = 53/62 (85%), Positives = 57/62 (91%)
Frame = +1
Query: 652 LDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMXTXXASTSLE 831
LDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ T S+SLE
Sbjct: 178 LDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLE 237
Query: 832 KS 837
KS
Sbjct: 238 KS 239
Score = 85.4 bits (202), Expect = 9e-18
Identities = 57/138 (41%), Positives = 70/138 (50%), Gaps = 7/138 (5%)
Frame = +2
Query: 119 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG-------RDGRYGPERLLR 277
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G +D G E +
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 278 G*RGPEQERYPHSEVPHRARYHHQLG*HGEDLAPHLLQXXXXXXXXXXXXXXXXXXQPQG 457
RG +YP + H + E + H
Sbjct: 62 --RGILTLKYP---IEHGIVNNWD---DMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPK 113
Query: 458 PTGXKMTQIMFETFNSPA 511
KMTQI+FETFN+PA
Sbjct: 114 SNREKMTQIIFETFNAPA 131
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 134 bits (324), Expect = 2e-32
Identities = 67/142 (47%), Positives = 96/142 (67%), Gaps = 2/142 (1%)
Frame = +3
Query: 243 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEH 419
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + ++ L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 420 PVLLTEAPL-NPKGQQGRR*PRSCLRPSTPPPMYVAIQAVLSLYASGRTTGIVLDSGDGV 596
P+LLTE PL N + ++ + P + ++Q VL+LYAS RTTGIVL+ GDG+
Sbjct: 107 PLLLTEPPLANIRNKE--KIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGL 164
Query: 597 SHTVPIYEGYALPHAILRSGLG 662
+H+VPIY+G+++P AI + +G
Sbjct: 165 THSVPIYDGFSIPSAIQQEEIG 186
Score = 60.9 bits (141), Expect = 2e-10
Identities = 26/49 (53%), Positives = 36/49 (73%)
Frame = +1
Query: 655 DLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEM 801
++ GRD+TDYL L + G+ ++AE+EIVR+IKEK CYVA DF E+
Sbjct: 184 EIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEI 232
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +2
Query: 125 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 238
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 126 bits (303), Expect = 5e-30
Identities = 59/129 (45%), Positives = 88/129 (68%)
Frame = +3
Query: 264 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 443
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 444 LNPKGQQGRR*PRSCLRPSTPPPMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 623
+NP + + R +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE
Sbjct: 110 MNPVANREKMCETMFERYGF-GGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYES 168
Query: 624 YALPHAILR 650
L H + R
Sbjct: 169 VVLNHLVGR 177
Score = 63.7 bits (148), Expect = 3e-11
Identities = 29/62 (46%), Positives = 42/62 (67%)
Frame = +1
Query: 652 LDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMXTXXASTSLE 831
LD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E + +T L
Sbjct: 178 LDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLM 237
Query: 832 KS 837
++
Sbjct: 238 RN 239
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2
Query: 131 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 229
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 105 bits (251), Expect = 1e-23
Identities = 62/166 (37%), Positives = 89/166 (53%), Gaps = 12/166 (7%)
Frame = +3
Query: 183 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 350
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 351 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PRSCLRPSTPPPMYVAIQ 530
WD ME+ W + + LR PE+H LLTE PLNP + +Y+A+Q
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPP-ENRENTAEIMFESFNCAGLYIAVQ 160
Query: 531 AVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAI 644
AVL+L AS + TG V+DSGDGV+H +P+ EGY + +I
Sbjct: 161 AVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSI 206
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +2
Query: 137 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 220
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
Score = 32.7 bits (71), Expect = 0.071
Identities = 21/51 (41%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = +1
Query: 652 LDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALDFEQE 798
+ LAGRD+T ++ +L +R S TAER IKE+ CYV D +E
Sbjct: 209 MPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPDIVKE 254
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 60.1 bits (139), Expect = 4e-10
Identities = 32/108 (29%), Positives = 53/108 (49%)
Frame = +3
Query: 309 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PRSC 488
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP + + +
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKT-LETM 121
Query: 489 LRPSTPPPMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 632
P Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 122 FESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +2
Query: 122 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 295
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 58.0 bits (134), Expect = 2e-09
Identities = 33/134 (24%), Positives = 64/134 (47%), Gaps = 2/134 (1%)
Frame = +3
Query: 264 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 437
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 438 APLNPKGQQGRR*PRSCLRPSTPPPMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIY 617
NP+ + ++ + P Y+ QAV +A+ ++T +++D G + P+
Sbjct: 109 PSWNPQSVR-QQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVV 167
Query: 618 EGYALPHAILRSGL 659
+G + I + L
Sbjct: 168 DGLIIRKGIFKQSL 181
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +2
Query: 122 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 223
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 56.8 bits (131), Expect = 4e-09
Identities = 37/142 (26%), Positives = 68/142 (47%), Gaps = 3/142 (2%)
Frame = +3
Query: 255 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 431
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 432 TEAPLNPKGQQGRR*PRSCLRPSTPPPMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHT 605
TE NP + P + I + S Y + + ++GIVL+ G+ SH
Sbjct: 121 TEPLANPTYVRSTM-TELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHV 179
Query: 606 VPIYEGYALPHAILRSGLGWSR 671
+P+ G + R G S+
Sbjct: 180 IPVLNGERILSEAKRISWGGSQ 201
Score = 37.5 bits (83), Expect = 0.002
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +2
Query: 59 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 232
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 42.7 bits (96), Expect = 7e-05
Identities = 19/50 (38%), Positives = 35/50 (70%)
Frame = +1
Query: 652 LDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEM 801
+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV+ +F+++M
Sbjct: 186 VDVGGKLLTNYLKEVISYRKYNMME--ETYIVNEIKESVCYVSQNFKEDM 233
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 35.9 bits (79), Expect = 0.008
Identities = 29/117 (24%), Positives = 49/117 (41%), Gaps = 4/117 (3%)
Frame = +3
Query: 324 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKGQQGRR*PRSCL 491
PI+ G + +W+ ++ W H Y+ L P + +PV L P +
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLV-IPTYWSLYDRELATQFFF 170
Query: 492 RPSTPPPMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRSGLG 662
P +A + ++ LYA G G+V+D G + PI +G + A + LG
Sbjct: 171 EECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLG 227
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -2
Query: 347 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 234
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,331,761
Number of Sequences: 5004
Number of extensions: 67611
Number of successful extensions: 249
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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