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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_D09
         (903 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_43840| Best HMM Match : NDK (HMM E-Value=0)                        205   3e-53
SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.)              71   1e-12
SB_49189| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.42 
SB_42687| Best HMM Match : UPF0262 (HMM E-Value=2.1)                   30   2.2  
SB_43132| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.2  

>SB_43840| Best HMM Match : NDK (HMM E-Value=0)
          Length = 786

 Score =  205 bits (501), Expect = 3e-53
 Identities = 94/147 (63%), Positives = 109/147 (74%)
 Frame = +3

Query: 162  ERTXIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRFFFPGL 341
            ERT +M+KPD V RGL+G II RFEKKGFKLV +KFV  SE+  ++HY  LA   F+ GL
Sbjct: 639  ERTFLMIKPDAVSRGLIGEIISRFEKKGFKLVAMKFVKKSEDHFRKHYESLAKLKFYDGL 698

Query: 342  VKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPXDSQPGTIRGDLCIQVGRNIIHGSDSVE 521
             KYMS  PV  MVWEGL VVKT R MLG T+P  S PGTIRGD  I +GRNIIHGSD+VE
Sbjct: 699  CKYMSQTPVCAMVWEGLGVVKTARVMLGETDPAKSLPGTIRGDFSIHIGRNIIHGSDAVE 758

Query: 522  SAKKEIGLWFTDKEVVGWTPANENWVY 602
            +AK+EI LWF D E+V WTP N  W+Y
Sbjct: 759  TAKEEIALWFKDDELVDWTPCNNPWMY 785



 Score =  120 bits (288), Expect = 2e-27
 Identities = 65/109 (59%), Positives = 75/109 (68%)
 Frame = +3

Query: 162 ERTXIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRFFFPGL 341
           ERT IMVKPDGVQRGLVG II+RFE+KGFKLV LK V  SEE L++HY+DLA   F+PGL
Sbjct: 127 ERTFIMVKPDGVQRGLVGEIIKRFEQKGFKLVALKMVQESEEHLKKHYADLAHLPFYPGL 186

Query: 342 VKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPXDSQPGTIRGDLCIQVG 488
           VK+MSSGPVV M                     DS+PGTIRGD C+ +G
Sbjct: 187 VKFMSSGPVVAMA--------------------DSKPGTIRGDFCVHIG 215


>SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1256

 Score = 70.9 bits (166), Expect = 1e-12
 Identities = 45/139 (32%), Positives = 73/139 (52%), Gaps = 3/139 (2%)
 Frame = +3

Query: 162  ERTXIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRFFFPGL 341
            + T  ++KP+ V+      I +R ++ GFK+   K V  S+EL  Q Y +   + FF GL
Sbjct: 673  QSTVAVIKPE-VEPDQRELIKQRIKEAGFKIQLQKEVTLSKELASQFYHEHEGKDFFEGL 731

Query: 342  VKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPXDSQ---PGTIRGDLCIQVGRNIIHGSD 512
              YMSSGP + MV    + V   R ++G  +P  ++   P +IR  L   V +N++HG  
Sbjct: 732  TDYMSSGPTMFMVLSKEDAVSGWRSLMGPVDPEQAKEMAPESIRAALGKDVMKNVVHGPS 791

Query: 513  SVESAKKEIGLWFTDKEVV 569
              E A K I  +F + +++
Sbjct: 792  DPEKAGKVIKEFFPEAKIL 810



 Score = 63.3 bits (147), Expect = 3e-10
 Identities = 40/140 (28%), Positives = 74/140 (52%), Gaps = 4/140 (2%)
 Frame = +3

Query: 162 ERTXIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRFFFPGL 341
           +RT  +++PD + R    +I+ + ++ GF++   K +  + E  ++ YS+   + FF  L
Sbjct: 538 QRTLALIRPDAL-RSRRESIMSKIQEAGFEIAMSKEMHLTREQAEEFYSEHKDQEFFDTL 596

Query: 342 VKYMSSGPVVPMVWEGLNVVKTGRQMLG---ATNPXDSQPGTIRGDLCIQVGR-NIIHGS 509
           V  MSSGP++ +     + ++  R MLG        D  P ++R    ++    N +HGS
Sbjct: 597 VTNMSSGPMMALCLAREDAIEGWRGMLGPKEVEKAKDEAPESLRAQFQVEDSPINPLHGS 656

Query: 510 DSVESAKKEIGLWFTDKEVV 569
           D+ E+A+KEI  +F  +  V
Sbjct: 657 DTAENAEKEIQKFFPMQSTV 676



 Score = 28.7 bits (61), Expect = 6.8
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 492 NIIHGSDSVESAKKEIGLWFTD 557
           N +HG DS ESA +E+  +F D
Sbjct: 501 NAVHGCDSNESAARELAFFFPD 522


>SB_49189| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1373

 Score = 32.7 bits (71), Expect = 0.42
 Identities = 15/31 (48%), Positives = 19/31 (61%)
 Frame = -3

Query: 412 RPVFTTLRPSHTIGTTGPELMYFTRPGKKNR 320
           R VF T+   HT+ T G E +YF RP K+ R
Sbjct: 194 RAVFNTMVVLHTVLTKGEETLYFERPIKRPR 224


>SB_42687| Best HMM Match : UPF0262 (HMM E-Value=2.1)
          Length = 908

 Score = 30.3 bits (65), Expect = 2.2
 Identities = 13/31 (41%), Positives = 19/31 (61%)
 Frame = -3

Query: 412 RPVFTTLRPSHTIGTTGPELMYFTRPGKKNR 320
           R VF T+   HT+ T G + +YF RP ++ R
Sbjct: 759 RAVFNTMVVLHTVLTKGEQTLYFERPIRRPR 789


>SB_43132| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 190

 Score = 30.3 bits (65), Expect = 2.2
 Identities = 13/31 (41%), Positives = 19/31 (61%)
 Frame = -3

Query: 412 RPVFTTLRPSHTIGTTGPELMYFTRPGKKNR 320
           R VF T+   HT+ T G + +YF RP ++ R
Sbjct: 46  RAVFNTMVVLHTVLTKGEQTLYFERPIRRPR 76


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,876,712
Number of Sequences: 59808
Number of extensions: 374118
Number of successful extensions: 710
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 683
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 708
length of database: 16,821,457
effective HSP length: 82
effective length of database: 11,917,201
effective search space used: 2597949818
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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