Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_D08
         (863 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   303   2e-83
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   298   6e-82
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   152   7e-38
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   125   9e-30
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.9  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.9  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  303 bits (744), Expect = 2e-83
 Identities = 132/196 (67%), Positives = 160/196 (81%)
 Frame = +2

Query: 176 HVGQAGVQXGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVF 355
           HVGQAG Q GNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK+VPR+++
Sbjct: 8   HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67

Query: 356 VDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLAD 535
           VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD
Sbjct: 68  VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127

Query: 536 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNS 715
            C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS
Sbjct: 128 NCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNS 187

Query: 716 ILTTHTTLEHSDCAFM 763
           +LTTH TL+ +DC FM
Sbjct: 188 VLTTHATLDLADCTFM 203



 Score = 56.4 bits (130), Expect = 5e-09
 Identities = 25/33 (75%), Positives = 27/33 (81%)
 Frame = +3

Query: 765 VDNEXIYDICRRNLDIERPTXTNLNRLIGXIVS 863
           VDNE  YDICRRNLDIERP+  NLNRLI  +VS
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLIAQVVS 236


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  298 bits (732), Expect = 6e-82
 Identities = 133/201 (66%), Positives = 163/201 (81%), Gaps = 5/201 (2%)
 Frame = +2

Query: 176 HVGQAGVQXGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHV 340
           HVGQAGVQ GNACWELYCLEHGI PDG  PT+     K     +D F TFFSETG GK V
Sbjct: 8   HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66

Query: 341 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 520
           PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67  PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126

Query: 521 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 700
           R++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAPQVST+VV
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV 186

Query: 701 EPYNSILTTHTTLEHSDCAFM 763
           EPYNS+LTTH TL++SDC FM
Sbjct: 187 EPYNSVLTTHATLDNSDCTFM 207



 Score = 58.0 bits (134), Expect = 2e-09
 Identities = 26/33 (78%), Positives = 27/33 (81%)
 Frame = +3

Query: 765 VDNEXIYDICRRNLDIERPTXTNLNRLIGXIVS 863
           VDNE  YDICRRNLDIERPT  NLNRLI  +VS
Sbjct: 208 VDNEACYDICRRNLDIERPTYENLNRLIAQVVS 240


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  152 bits (368), Expect = 7e-38
 Identities = 76/193 (39%), Positives = 107/193 (55%)
 Frame = +2

Query: 182 GQAGVQXGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 361
           GQ G Q G A W     EHG+   G      T     +  N +F+E   GK+VPRAV VD
Sbjct: 10  GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67

Query: 362 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 541
           LEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 68  LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127

Query: 542 TGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSIL 721
             LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  VVEPYN+ L
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187

Query: 722 TTHTTLEHSDCAF 760
           + H  +E+SD  F
Sbjct: 188 SMHQLVENSDETF 200


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  125 bits (301), Expect = 9e-30
 Identities = 68/183 (37%), Positives = 104/183 (56%), Gaps = 3/183 (1%)
 Frame = +2

Query: 182 GQAGVQXGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 361
           GQ G Q G+  W+  CLEHGI PDG + +  T   G D  + FF ++   +++PRA+ +D
Sbjct: 11  GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68

Query: 362 LEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLAD 535
           LEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++D I + AD
Sbjct: 69  LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127

Query: 536 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYN 712
               L+GF + H             L+ERL+  Y KK    ++++P  Q VS  VV+PYN
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187

Query: 713 SIL 721
           S+L
Sbjct: 188 SLL 190


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 234 STASSLMARCPQTRPSGVETILSTLSSARPELAST 338
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 253 WPDAHRQDHRGWRRFFQHFLQR 318
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,134,520
Number of Sequences: 5004
Number of extensions: 60384
Number of successful extensions: 168
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)