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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_D01
         (858 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-...   217   8e-57
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei...    47   1e-05
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei...    46   3e-05
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei...    45   7e-05
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei...    45   7e-05

>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
           beta-alanine synthase, putative similar to beta-alanine
           synthase [Dictyostelium discoideum] GI:14334061;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 408

 Score =  217 bits (530), Expect = 8e-57
 Identities = 101/165 (61%), Positives = 122/165 (73%)
 Frame = +2

Query: 278 FPAKDEQTRPPRIVKVGVIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGXEGVNIICFQ 457
           F A  EQ R PR+V+VG+IQ+SIA+PT  P ++Q + IF+K+K IID AG  GVNI+C Q
Sbjct: 77  FSADKEQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQ 136

Query: 458 ELWNMPFAFCTREKQPWCEFAESGEDGATTTFLRELAIXYAMVIVSSILXRDXXHSDILW 637
           E W MPFAFCTRE++ WCEFAE   DG +T FL+ELA  Y MVIVS IL RD  H ++LW
Sbjct: 137 EAWTMPFAFCTRERR-WCEFAEP-VDGESTKFLQELAKKYNMVIVSPILERDIDHGEVLW 194

Query: 638 NXXVVIXDXGNVIGKHRXXHIPXXGDFNXSXYYMEXNTGHPVFXT 772
           N  V+I + GN+IGKHR  HIP  GDFN S YYME +TGHPVF T
Sbjct: 195 NTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFET 239


>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
           low similarity to beta-alanine synthase [Drosophila
           melanogaster] GI:14334063; contains Pfam profile
           PF00795: hydrolase, carbon-nitrogen family
          Length = 299

 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 33/124 (26%), Positives = 60/124 (48%), Gaps = 1/124 (0%)
 Frame = +2

Query: 404 KKIIDVAGXEGVNIICFQELWNMPFAFCTREKQPWCEFAESGEDGATTTFLRELAIXYAM 583
           ++++  A  +G NII  QEL+   + FC  +++ + + A+  ++  T   +++LA    +
Sbjct: 31  ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89

Query: 584 VI-VSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHIPXXGDFNXSXYYMEXNTGHP 760
           VI VS     +  H    +N   +I   G  +G +R  HIP    +    Y+   +TG  
Sbjct: 90  VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 145

Query: 761 VFXT 772
           VF T
Sbjct: 146 VFQT 149


>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
           low similarity to beta-alanine synthase [Drosophila
           melanogaster] GI:14334063; contains Pfam profile
           PF00795: hydrolase, carbon-nitrogen family
          Length = 326

 Score = 46.0 bits (104), Expect = 3e-05
 Identities = 33/122 (27%), Positives = 58/122 (47%), Gaps = 1/122 (0%)
 Frame = +2

Query: 410 IIDVAGXEGVNIICFQELWNMPFAFCTREKQPWCEFAESGEDGATTTFLRELAIXYAMVI 589
           ++  A  +G NII  QEL+   + FC  +++ + + A+  ++  T   +++LA    +VI
Sbjct: 60  LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118

Query: 590 -VSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHIPXXGDFNXSXYYMEXNTGHPVF 766
            VS     +  H    +N   +I   G  +G +R  HIP    +    Y+   +TG  VF
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174

Query: 767 XT 772
            T
Sbjct: 175 QT 176


>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 294

 Score = 44.8 bits (101), Expect = 7e-05
 Identities = 29/113 (25%), Positives = 51/113 (45%), Gaps = 2/113 (1%)
 Frame = +2

Query: 368 VNEQKKAIFNKVKKIIDVAGXEGVNIICFQELWNMPFAFCTREKQP-WCEFAESGEDGA- 541
           V   KK   +  KK I+ A  +G  ++   E+WN P+   + +  P + E  ++G D + 
Sbjct: 97  VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153

Query: 542 TTTFLRELAIXYAMVIVSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHI 700
           +T  L E++    + I+   +       D L+N   V    G +  KHR  H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSIPERV--GDRLYNTCCVFGSDGELKAKHRKIHL 204


>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 369

 Score = 44.8 bits (101), Expect = 7e-05
 Identities = 29/113 (25%), Positives = 51/113 (45%), Gaps = 2/113 (1%)
 Frame = +2

Query: 368 VNEQKKAIFNKVKKIIDVAGXEGVNIICFQELWNMPFAFCTREKQP-WCEFAESGEDGA- 541
           V   KK   +  KK I+ A  +G  ++   E+WN P+   + +  P + E  ++G D + 
Sbjct: 97  VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153

Query: 542 TTTFLRELAIXYAMVIVSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHI 700
           +T  L E++    + I+   +       D L+N   V    G +  KHR  H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSIPERV--GDRLYNTCCVFGSDGELKAKHRKIHL 204


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,613,992
Number of Sequences: 28952
Number of extensions: 202794
Number of successful extensions: 421
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 408
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 417
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1999652000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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