BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_D01 (858 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 217 8e-57 At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 47 1e-05 At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 46 3e-05 At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 45 7e-05 At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 45 7e-05 >At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-alanine synthase, putative similar to beta-alanine synthase [Dictyostelium discoideum] GI:14334061; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 408 Score = 217 bits (530), Expect = 8e-57 Identities = 101/165 (61%), Positives = 122/165 (73%) Frame = +2 Query: 278 FPAKDEQTRPPRIVKVGVIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGXEGVNIICFQ 457 F A EQ R PR+V+VG+IQ+SIA+PT P ++Q + IF+K+K IID AG GVNI+C Q Sbjct: 77 FSADKEQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQ 136 Query: 458 ELWNMPFAFCTREKQPWCEFAESGEDGATTTFLRELAIXYAMVIVSSILXRDXXHSDILW 637 E W MPFAFCTRE++ WCEFAE DG +T FL+ELA Y MVIVS IL RD H ++LW Sbjct: 137 EAWTMPFAFCTRERR-WCEFAEP-VDGESTKFLQELAKKYNMVIVSPILERDIDHGEVLW 194 Query: 638 NXXVVIXDXGNVIGKHRXXHIPXXGDFNXSXYYMEXNTGHPVFXT 772 N V+I + GN+IGKHR HIP GDFN S YYME +TGHPVF T Sbjct: 195 NTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFET 239 >At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein low similarity to beta-alanine synthase [Drosophila melanogaster] GI:14334063; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 299 Score = 47.2 bits (107), Expect = 1e-05 Identities = 33/124 (26%), Positives = 60/124 (48%), Gaps = 1/124 (0%) Frame = +2 Query: 404 KKIIDVAGXEGVNIICFQELWNMPFAFCTREKQPWCEFAESGEDGATTTFLRELAIXYAM 583 ++++ A +G NII QEL+ + FC +++ + + A+ ++ T +++LA + Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89 Query: 584 VI-VSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHIPXXGDFNXSXYYMEXNTGHP 760 VI VS + H +N +I G +G +R HIP + Y+ +TG Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 145 Query: 761 VFXT 772 VF T Sbjct: 146 VFQT 149 >At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein low similarity to beta-alanine synthase [Drosophila melanogaster] GI:14334063; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 326 Score = 46.0 bits (104), Expect = 3e-05 Identities = 33/122 (27%), Positives = 58/122 (47%), Gaps = 1/122 (0%) Frame = +2 Query: 410 IIDVAGXEGVNIICFQELWNMPFAFCTREKQPWCEFAESGEDGATTTFLRELAIXYAMVI 589 ++ A +G NII QEL+ + FC +++ + + A+ ++ T +++LA +VI Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118 Query: 590 -VSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHIPXXGDFNXSXYYMEXNTGHPVF 766 VS + H +N +I G +G +R HIP + Y+ +TG VF Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174 Query: 767 XT 772 T Sbjct: 175 QT 176 >At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein similar to Nit protein 2 [Homo sapiens] GI:9367116; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 294 Score = 44.8 bits (101), Expect = 7e-05 Identities = 29/113 (25%), Positives = 51/113 (45%), Gaps = 2/113 (1%) Frame = +2 Query: 368 VNEQKKAIFNKVKKIIDVAGXEGVNIICFQELWNMPFAFCTREKQP-WCEFAESGEDGA- 541 V KK + KK I+ A +G ++ E+WN P+ + + P + E ++G D + Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153 Query: 542 TTTFLRELAIXYAMVIVSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHI 700 +T L E++ + I+ + D L+N V G + KHR H+ Sbjct: 154 STAMLSEVSKRLKITIIGGSIPERV--GDRLYNTCCVFGSDGELKAKHRKIHL 204 >At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein similar to Nit protein 2 [Homo sapiens] GI:9367116; contains Pfam profile PF00795: hydrolase, carbon-nitrogen family Length = 369 Score = 44.8 bits (101), Expect = 7e-05 Identities = 29/113 (25%), Positives = 51/113 (45%), Gaps = 2/113 (1%) Frame = +2 Query: 368 VNEQKKAIFNKVKKIIDVAGXEGVNIICFQELWNMPFAFCTREKQP-WCEFAESGEDGA- 541 V KK + KK I+ A +G ++ E+WN P+ + + P + E ++G D + Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153 Query: 542 TTTFLRELAIXYAMVIVSSILXRDXXHSDILWNXXVVIXDXGNVIGKHRXXHI 700 +T L E++ + I+ + D L+N V G + KHR H+ Sbjct: 154 STAMLSEVSKRLKITIIGGSIPERV--GDRLYNTCCVFGSDGELKAKHRKIHL 204 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,613,992 Number of Sequences: 28952 Number of extensions: 202794 Number of successful extensions: 421 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 408 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 417 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1999652000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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