BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_C22
(886 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|... 134 2e-32
SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12... 134 2e-32
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 27 4.7
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 26 6.2
>SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein
L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual
Length = 165
Score = 134 bits (323), Expect = 2e-32
Identities = 63/107 (58%), Positives = 83/107 (77%)
Frame = +2
Query: 176 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATSDWKGLKITV 355
MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT DWKGL++TV
Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60
Query: 356 QLTVQNRXAQIAVVPSGSSPYHQSP*GASPVTVKSRKISKHNGNISL 496
+LT+QNR A ++VVPS S+ ++ + K + ++ H+GN+SL
Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVA-HSGNVSL 106
Score = 37.9 bits (84), Expect = 0.002
Identities = 19/39 (48%), Positives = 27/39 (69%)
Frame = +3
Query: 495 LEDVVGIAKIMRNRSXGPGTFLASVKEILGTAQSVGCTV 611
L++++ +A+ MR +S +VKEILGTA SVGCTV
Sbjct: 106 LDEIIEVARTMRFKSLAK-ELSGTVKEILGTAFSVGCTV 143
>SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein
L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual
Length = 165
Score = 134 bits (323), Expect = 2e-32
Identities = 63/107 (58%), Positives = 83/107 (77%)
Frame = +2
Query: 176 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATSDWKGLKITV 355
MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT DWKGL++TV
Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60
Query: 356 QLTVQNRXAQIAVVPSGSSPYHQSP*GASPVTVKSRKISKHNGNISL 496
+LT+QNR A ++VVPS S+ ++ + K + ++ H+GN+SL
Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVA-HSGNVSL 106
Score = 37.9 bits (84), Expect = 0.002
Identities = 19/39 (48%), Positives = 27/39 (69%)
Frame = +3
Query: 495 LEDVVGIAKIMRNRSXGPGTFLASVKEILGTAQSVGCTV 611
L++++ +A+ MR +S +VKEILGTA SVGCTV
Sbjct: 106 LDEIIEVARTMRFKSLAK-ELSGTVKEILGTAFSVGCTV 143
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 26.6 bits (56), Expect = 4.7
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -1
Query: 523 IFAMPTTSSKGDVAVVF*YFSAFYGHRGGSLRALMIRAAARGNNSYL 383
+F PT+SSK D V S+F+ + G + AL + + GN L
Sbjct: 480 VFYNPTSSSKNDAFVA---HSSFFANEGDIITALNVFESFVGNKKDL 523
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/46 (23%), Positives = 24/46 (52%)
Frame = -3
Query: 152 LYYNNVKSKWMSLDSARATA*TFRDVGSSL*KEKQKIKILRIPYSE 15
L+ N + +W SL+ + + +D S+ + IL++PY++
Sbjct: 189 LFSNEIPQEWCSLELSYYKSSPSKDFSSASQPNSEGFSILKLPYNK 234
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,986,249
Number of Sequences: 5004
Number of extensions: 59675
Number of successful extensions: 143
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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