BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_C22 (886 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|... 134 2e-32 SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12... 134 2e-32 SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 27 4.7 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 26 6.2 >SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 134 bits (323), Expect = 2e-32 Identities = 63/107 (58%), Positives = 83/107 (77%) Frame = +2 Query: 176 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATSDWKGLKITV 355 MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT DWKGL++TV Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60 Query: 356 QLTVQNRXAQIAVVPSGSSPYHQSP*GASPVTVKSRKISKHNGNISL 496 +LT+QNR A ++VVPS S+ ++ + K + ++ H+GN+SL Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVA-HSGNVSL 106 Score = 37.9 bits (84), Expect = 0.002 Identities = 19/39 (48%), Positives = 27/39 (69%) Frame = +3 Query: 495 LEDVVGIAKIMRNRSXGPGTFLASVKEILGTAQSVGCTV 611 L++++ +A+ MR +S +VKEILGTA SVGCTV Sbjct: 106 LDEIIEVARTMRFKSLAK-ELSGTVKEILGTAFSVGCTV 143 >SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 134 bits (323), Expect = 2e-32 Identities = 63/107 (58%), Positives = 83/107 (77%) Frame = +2 Query: 176 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATSDWKGLKITV 355 MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT DWKGL++TV Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60 Query: 356 QLTVQNRXAQIAVVPSGSSPYHQSP*GASPVTVKSRKISKHNGNISL 496 +LT+QNR A ++VVPS S+ ++ + K + ++ H+GN+SL Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVA-HSGNVSL 106 Score = 37.9 bits (84), Expect = 0.002 Identities = 19/39 (48%), Positives = 27/39 (69%) Frame = +3 Query: 495 LEDVVGIAKIMRNRSXGPGTFLASVKEILGTAQSVGCTV 611 L++++ +A+ MR +S +VKEILGTA SVGCTV Sbjct: 106 LDEIIEVARTMRFKSLAK-ELSGTVKEILGTAFSVGCTV 143 >SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 26.6 bits (56), Expect = 4.7 Identities = 16/47 (34%), Positives = 24/47 (51%) Frame = -1 Query: 523 IFAMPTTSSKGDVAVVF*YFSAFYGHRGGSLRALMIRAAARGNNSYL 383 +F PT+SSK D V S+F+ + G + AL + + GN L Sbjct: 480 VFYNPTSSSKNDAFVA---HSSFFANEGDIITALNVFESFVGNKKDL 523 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 26.2 bits (55), Expect = 6.2 Identities = 11/46 (23%), Positives = 24/46 (52%) Frame = -3 Query: 152 LYYNNVKSKWMSLDSARATA*TFRDVGSSL*KEKQKIKILRIPYSE 15 L+ N + +W SL+ + + +D S+ + IL++PY++ Sbjct: 189 LFSNEIPQEWCSLELSYYKSSPSKDFSSASQPNSEGFSILKLPYNK 234 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,986,249 Number of Sequences: 5004 Number of extensions: 59675 Number of successful extensions: 143 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 444486180 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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