BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_C17 (904 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 138 1e-33 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 138 1e-33 SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe... 27 3.6 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 138 bits (334), Expect = 1e-33 Identities = 61/90 (67%), Positives = 69/90 (76%) Frame = +2 Query: 293 RNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPT 472 +N RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPT Sbjct: 46 KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPT 105 Query: 473 KPWRRWHRRVNLRTAESGLGSSRCCYRRPS 562 K WR+WH +VN + S+ PS Sbjct: 106 KTWRKWHVKVNQNEKRYAIASAVAASGVPS 135 Score = 115 bits (277), Expect = 8e-27 Identities = 53/92 (57%), Positives = 70/92 (76%) Frame = +3 Query: 573 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 752 ARGH IE+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RN Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRN 198 Query: 753 RRRIQRKGPS*SSTRIRGLTRAFRNIPGVELL 848 RR +QR+GP G+ +AFRNIPGVE++ Sbjct: 199 RRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIV 230 Score = 37.9 bits (84), Expect = 0.002 Identities = 18/34 (52%), Positives = 24/34 (70%), Gaps = 1/34 (2%) Frame = +1 Query: 232 FVFKAPIRPDLVNDVHVSMSK-KLEAALLREQGG 330 FVFKAPIRPDLV VH +++K K + + E+ G Sbjct: 26 FVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 138 bits (334), Expect = 1e-33 Identities = 61/90 (67%), Positives = 69/90 (76%) Frame = +2 Query: 293 RNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPT 472 +N RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPT Sbjct: 46 KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPT 105 Query: 473 KPWRRWHRRVNLRTAESGLGSSRCCYRRPS 562 K WR+WH +VN + S+ PS Sbjct: 106 KTWRKWHVKVNQNEKRYAISSAVAASGVPS 135 Score = 115 bits (276), Expect = 1e-26 Identities = 53/92 (57%), Positives = 70/92 (76%) Frame = +3 Query: 573 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 752 ARGH IE+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RN Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRN 198 Query: 753 RRRIQRKGPS*SSTRIRGLTRAFRNIPGVELL 848 RR +QR+GP G+ +AFRNIPGVE++ Sbjct: 199 RRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIV 230 Score = 37.9 bits (84), Expect = 0.002 Identities = 18/34 (52%), Positives = 24/34 (70%), Gaps = 1/34 (2%) Frame = +1 Query: 232 FVFKAPIRPDLVNDVHVSMSK-KLEAALLREQGG 330 FVFKAPIRPDLV VH +++K K + + E+ G Sbjct: 26 FVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59 >SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 425 Score = 27.1 bits (57), Expect = 3.6 Identities = 20/67 (29%), Positives = 27/67 (40%), Gaps = 1/67 (1%) Frame = +2 Query: 239 SRRPYVRTWSMMFTFLCPRNSRQPYCVSKEAGHQTSAESWGTGRAVARI-PRVRGGGTHR 415 SRR WS MFT P + P+ + A T +G G +AR+ R GG Sbjct: 342 SRRNIPLVWSYMFTTASPTLTDDPHDI-VSANSTTPMAGFGFGLPLARLYTRYFGGDLEL 400 Query: 416 SGQGAFG 436 +G Sbjct: 401 ISMEGYG 407 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,288,939 Number of Sequences: 5004 Number of extensions: 61754 Number of successful extensions: 145 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 140 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 456499320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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