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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_C17
         (904 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   138   1e-33
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   138   1e-33
SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe...    27   3.6  

>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  138 bits (334), Expect = 1e-33
 Identities = 61/90 (67%), Positives = 69/90 (76%)
 Frame = +2

Query: 293 RNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPT 472
           +N RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPT
Sbjct: 46  KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPT 105

Query: 473 KPWRRWHRRVNLRTAESGLGSSRCCYRRPS 562
           K WR+WH +VN       + S+      PS
Sbjct: 106 KTWRKWHVKVNQNEKRYAIASAVAASGVPS 135



 Score =  115 bits (277), Expect = 8e-27
 Identities = 53/92 (57%), Positives = 70/92 (76%)
 Frame = +3

Query: 573 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 752
           ARGH IE+IPE+PLVV D VQ   KTK+AV  L+ +KA+ D++KV  S++LRAGKGK+RN
Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRN 198

Query: 753 RRRIQRKGPS*SSTRIRGLTRAFRNIPGVELL 848
           RR +QR+GP        G+ +AFRNIPGVE++
Sbjct: 199 RRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIV 230



 Score = 37.9 bits (84), Expect = 0.002
 Identities = 18/34 (52%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
 Frame = +1

Query: 232 FVFKAPIRPDLVNDVHVSMSK-KLEAALLREQGG 330
           FVFKAPIRPDLV  VH +++K K +   + E+ G
Sbjct: 26  FVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59


>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  138 bits (334), Expect = 1e-33
 Identities = 61/90 (67%), Positives = 69/90 (76%)
 Frame = +2

Query: 293 RNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPT 472
           +N RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPT
Sbjct: 46  KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPT 105

Query: 473 KPWRRWHRRVNLRTAESGLGSSRCCYRRPS 562
           K WR+WH +VN       + S+      PS
Sbjct: 106 KTWRKWHVKVNQNEKRYAISSAVAASGVPS 135



 Score =  115 bits (276), Expect = 1e-26
 Identities = 53/92 (57%), Positives = 70/92 (76%)
 Frame = +3

Query: 573 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 752
           ARGH IE+IPE+PLVV D VQ   KTK+AV  L+ +KA+ D++KV  S++LRAGKGK+RN
Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRN 198

Query: 753 RRRIQRKGPS*SSTRIRGLTRAFRNIPGVELL 848
           RR +QR+GP        G+ +AFRNIPGVE++
Sbjct: 199 RRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIV 230



 Score = 37.9 bits (84), Expect = 0.002
 Identities = 18/34 (52%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
 Frame = +1

Query: 232 FVFKAPIRPDLVNDVHVSMSK-KLEAALLREQGG 330
           FVFKAPIRPDLV  VH +++K K +   + E+ G
Sbjct: 26  FVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59


>SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 425

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 20/67 (29%), Positives = 27/67 (40%), Gaps = 1/67 (1%)
 Frame = +2

Query: 239 SRRPYVRTWSMMFTFLCPRNSRQPYCVSKEAGHQTSAESWGTGRAVARI-PRVRGGGTHR 415
           SRR     WS MFT   P  +  P+ +   A   T    +G G  +AR+  R  GG    
Sbjct: 342 SRRNIPLVWSYMFTTASPTLTDDPHDI-VSANSTTPMAGFGFGLPLARLYTRYFGGDLEL 400

Query: 416 SGQGAFG 436
                +G
Sbjct: 401 ISMEGYG 407


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,288,939
Number of Sequences: 5004
Number of extensions: 61754
Number of successful extensions: 145
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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