BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_C17
(904 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 138 1e-33
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 138 1e-33
SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe... 27 3.6
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 138 bits (334), Expect = 1e-33
Identities = 61/90 (67%), Positives = 69/90 (76%)
Frame = +2
Query: 293 RNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPT 472
+N RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPT
Sbjct: 46 KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPT 105
Query: 473 KPWRRWHRRVNLRTAESGLGSSRCCYRRPS 562
K WR+WH +VN + S+ PS
Sbjct: 106 KTWRKWHVKVNQNEKRYAIASAVAASGVPS 135
Score = 115 bits (277), Expect = 8e-27
Identities = 53/92 (57%), Positives = 70/92 (76%)
Frame = +3
Query: 573 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 752
ARGH IE+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RN
Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRN 198
Query: 753 RRRIQRKGPS*SSTRIRGLTRAFRNIPGVELL 848
RR +QR+GP G+ +AFRNIPGVE++
Sbjct: 199 RRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIV 230
Score = 37.9 bits (84), Expect = 0.002
Identities = 18/34 (52%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
Frame = +1
Query: 232 FVFKAPIRPDLVNDVHVSMSK-KLEAALLREQGG 330
FVFKAPIRPDLV VH +++K K + + E+ G
Sbjct: 26 FVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 138 bits (334), Expect = 1e-33
Identities = 61/90 (67%), Positives = 69/90 (76%)
Frame = +2
Query: 293 RNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPT 472
+N RQPY VS++AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPT
Sbjct: 46 KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPT 105
Query: 473 KPWRRWHRRVNLRTAESGLGSSRCCYRRPS 562
K WR+WH +VN + S+ PS
Sbjct: 106 KTWRKWHVKVNQNEKRYAISSAVAASGVPS 135
Score = 115 bits (276), Expect = 1e-26
Identities = 53/92 (57%), Positives = 70/92 (76%)
Frame = +3
Query: 573 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 752
ARGH IE+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RN
Sbjct: 139 ARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRN 198
Query: 753 RRRIQRKGPS*SSTRIRGLTRAFRNIPGVELL 848
RR +QR+GP G+ +AFRNIPGVE++
Sbjct: 199 RRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIV 230
Score = 37.9 bits (84), Expect = 0.002
Identities = 18/34 (52%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
Frame = +1
Query: 232 FVFKAPIRPDLVNDVHVSMSK-KLEAALLREQGG 330
FVFKAPIRPDLV VH +++K K + + E+ G
Sbjct: 26 FVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59
>SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 27.1 bits (57), Expect = 3.6
Identities = 20/67 (29%), Positives = 27/67 (40%), Gaps = 1/67 (1%)
Frame = +2
Query: 239 SRRPYVRTWSMMFTFLCPRNSRQPYCVSKEAGHQTSAESWGTGRAVARI-PRVRGGGTHR 415
SRR WS MFT P + P+ + A T +G G +AR+ R GG
Sbjct: 342 SRRNIPLVWSYMFTTASPTLTDDPHDI-VSANSTTPMAGFGFGLPLARLYTRYFGGDLEL 400
Query: 416 SGQGAFG 436
+G
Sbjct: 401 ISMEGYG 407
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,288,939
Number of Sequences: 5004
Number of extensions: 61754
Number of successful extensions: 145
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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