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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_B24
         (904 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote...    32   0.45 
At1g22060.1 68414.m02759 expressed protein                             31   1.0  
At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative severa...    29   4.2  
At5g24740.1 68418.m02920 expressed protein                             29   5.6  
At5g24440.1 68418.m02880 RNA-binding protein, putative                 28   7.4  

>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
           protein-like (HSP26.5-P) contains Pfam profile: PF00011
           Hsp20/alpha crystallin family; identified in Scharf,
           K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 232

 Score = 32.3 bits (70), Expect = 0.45
 Identities = 13/79 (16%), Positives = 41/79 (51%)
 Frame = +2

Query: 218 FSPYVRESMLDTHSLWSNLANEMQHLDDMMKELSLNFXSIINEGRVEGDKYQISIHLPGY 397
           F+P + E    T  + + L    ++++ +    ++N   ++ + + + D Y++   +PG 
Sbjct: 88  FTPTLNEFFPPT--IGNTLIQATENMNRIFDNFNVNPFQLMGQVKEQDDCYKLRYEVPGL 145

Query: 398 EQKDINVKAKNGVLMVQAN 454
            ++D+ +   +G+L ++ +
Sbjct: 146 TKEDVKITVNDGILTIKGD 164


>At1g22060.1 68414.m02759 expressed protein
          Length = 1999

 Score = 31.1 bits (67), Expect = 1.0
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = +2

Query: 236  ESMLDTHSLWSNLANEMQHLDDMMKELSLNFXSIINE 346
            E MLDT   +S++  E++ + D   +LSL F  +  E
Sbjct: 1932 EEMLDTKGRYSSMETELREMHDRYSQLSLKFAEVEGE 1968


>At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative several
           putative ATP-dependent helicases
          Length = 1058

 Score = 29.1 bits (62), Expect = 4.2
 Identities = 20/98 (20%), Positives = 48/98 (48%), Gaps = 2/98 (2%)
 Frame = +2

Query: 353 VEGDKYQISIHLPGYEQKDINVKAKNGVLMVQANSAFNHYLKIQNLPWDVNSEGSWVYEK 532
           + G+   + +   G   +D+    K+      ++S F+ Y+K + L  D       + + 
Sbjct: 20  INGESTTVQVSSDG-TIRDLKKALKSCFPPASSSSNFHLYIKGEKLKLDTRVAAITINDG 78

Query: 533 DVLKITFPLKQKQXEDSKRP-VAEPTE-TXPTNVSREE 640
           D+L + FP K+K+   + +P +++P+  +  T+  ++E
Sbjct: 79  DILGL-FPFKKKELRQTPKPDLSKPSSLSSRTSTMKDE 115


>At5g24740.1 68418.m02920 expressed protein
          Length = 3306

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 12/47 (25%), Positives = 24/47 (51%)
 Frame = +2

Query: 503  NSEGSWVYEKDVLKITFPLKQKQXEDSKRPVAEPTETXPTNVSREEM 643
            NS+G W + +D  K + P K++    S +P+ +     P+    E++
Sbjct: 3232 NSDGVWNWRQDQQKKSSPTKKRWNNPSAQPLLQTNLEFPSEEEAEDL 3278


>At5g24440.1 68418.m02880 RNA-binding protein, putative
          Length = 320

 Score = 28.3 bits (60), Expect = 7.4
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = +2

Query: 269 NLANEMQHLDDMMKELSLNFXSIINEGRVEGDKYQISIHLP 391
           NL +E+ HL+ M KE   +F +  +     G+++  + H P
Sbjct: 58  NLKSEISHLNPMAKEFVPSFLAQTHHSEFWGNRFWFTNHFP 98


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,837,438
Number of Sequences: 28952
Number of extensions: 259292
Number of successful extensions: 722
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 694
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 719
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2129873112
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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