Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_B20
         (901 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P04142 Cluster: Cecropin-B precursor; n=16; Obtectomera...   126   8e-28
UniRef50_P01507 Cluster: Cecropin-A precursor; n=17; Ditrysia|Re...    92   2e-17
UniRef50_A6BMG0 Cluster: Cecropin A; n=1; Plutella xylostella|Re...    62   2e-08
UniRef50_Q2WGL2 Cluster: Antibacterial peptide; n=4; Obtectomera...    48   2e-04
UniRef50_P48821 Cluster: Antibacterial peptide enbocin precursor...    48   3e-04
UniRef50_Q5W8G6 Cluster: Cecropin; n=1; Acalolepta luxuriosa|Rep...    41   0.037
UniRef50_P01511 Cluster: Cecropin-D; n=6; Obtectomera|Rep: Cecro...    41   0.037
UniRef50_Q8MUF4 Cluster: Cecropin-B precursor; n=18; Culicidae|R...    40   0.065
UniRef50_Q3R5V3 Cluster: Putative uncharacterized protein precur...    33   7.5  

>UniRef50_P04142 Cluster: Cecropin-B precursor; n=16;
           Obtectomera|Rep: Cecropin-B precursor - Bombyx mori
           (Silk moth)
          Length = 63

 Score =  126 bits (304), Expect = 8e-28
 Identities = 62/63 (98%), Positives = 62/63 (98%)
 Frame = +1

Query: 136 MNFAKILSFVFALVLALSMTSAAPEPRWKIFKXIEKMGRNIRDGIVKAGPAIEVLGSAKA 315
           MNFAKILSFVFALVLALSMTSAAPEPRWKIFK IEKMGRNIRDGIVKAGPAIEVLGSAKA
Sbjct: 1   MNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEKMGRNIRDGIVKAGPAIEVLGSAKA 60

Query: 316 IGK 324
           IGK
Sbjct: 61  IGK 63


>UniRef50_P01507 Cluster: Cecropin-A precursor; n=17; Ditrysia|Rep:
           Cecropin-A precursor - Hyalophora cecropia (Cecropia
           moth)
          Length = 64

 Score = 92.3 bits (219), Expect = 2e-17
 Identities = 40/63 (63%), Positives = 52/63 (82%)
 Frame = +1

Query: 136 MNFAKILSFVFALVLALSMTSAAPEPRWKIFKXIEKMGRNIRDGIVKAGPAIEVLGSAKA 315
           MNF++I  FVFA + AL+M +AAPEP+WK+FK IEK+G+NIRDGI+KAGPA+ V+G A  
Sbjct: 1   MNFSRIFFFVFACLTALAMVNAAPEPKWKLFKKIEKVGQNIRDGIIKAGPAVAVVGQATQ 60

Query: 316 IGK 324
           I K
Sbjct: 61  IAK 63


>UniRef50_A6BMG0 Cluster: Cecropin A; n=1; Plutella xylostella|Rep:
           Cecropin A - Plutella xylostella (Diamondback moth)
          Length = 66

 Score = 61.7 bits (143), Expect = 2e-08
 Identities = 31/64 (48%), Positives = 43/64 (67%), Gaps = 1/64 (1%)
 Frame = +1

Query: 136 MNFAKILSFVFALVLALSMTSAAPEPRWKIFKXIEKMGRNIRDGIVK-AGPAIEVLGSAK 312
           M  + I  FVF    A++  SAAP  RWK FK +EK+GRNIR+GI++  GPA+ V+G A 
Sbjct: 1   MKLSNIFFFVFMAFFAVASVSAAP--RWKPFKKLEKVGRNIRNGIIRYNGPAVAVIGQAT 58

Query: 313 AIGK 324
           +I +
Sbjct: 59  SIAR 62


>UniRef50_Q2WGL2 Cluster: Antibacterial peptide; n=4;
           Obtectomera|Rep: Antibacterial peptide - Bombyx mori
           (Silk moth)
          Length = 66

 Score = 48.4 bits (110), Expect = 2e-04
 Identities = 25/61 (40%), Positives = 36/61 (59%)
 Frame = +1

Query: 136 MNFAKILSFVFALVLALSMTSAAPEPRWKIFKXIEKMGRNIRDGIVKAGPAIEVLGSAKA 315
           M F KI   VF  ++ + + S A    W  FK +E +G+ +RD I+ AGPAI+VL  AK 
Sbjct: 1   MYFTKI---VFVAIICIMIVSCASA--WDFFKELEGVGQRVRDSIISAGPAIDVLQKAKG 55

Query: 316 I 318
           +
Sbjct: 56  L 56


>UniRef50_P48821 Cluster: Antibacterial peptide enbocin precursor;
           n=5; Ditrysia|Rep: Antibacterial peptide enbocin
           precursor - Bombyx mori (Silk moth)
          Length = 59

 Score = 48.0 bits (109), Expect = 3e-04
 Identities = 23/61 (37%), Positives = 38/61 (62%)
 Frame = +1

Query: 136 MNFAKILSFVFALVLALSMTSAAPEPRWKIFKXIEKMGRNIRDGIVKAGPAIEVLGSAKA 315
           MNF +I+ F+F +V A    +A+ +P W IFK IE+     RD ++ AGPA+  + +A +
Sbjct: 1   MNFTRIIFFLFVVVFA----TASGKP-WNIFKEIERAVARTRDAVISAGPAVRTVAAATS 55

Query: 316 I 318
           +
Sbjct: 56  V 56


>UniRef50_Q5W8G6 Cluster: Cecropin; n=1; Acalolepta luxuriosa|Rep:
           Cecropin - Acalolepta luxuriosa (Udo longicorn beetle)
          Length = 60

 Score = 41.1 bits (92), Expect = 0.037
 Identities = 24/56 (42%), Positives = 34/56 (60%), Gaps = 1/56 (1%)
 Frame = +1

Query: 160 FVFALVLALSMTSAAPEPRWKIFKXIEKMGRNIRDGIVKAGP-AIEVLGSAKAIGK 324
           FVFAL + L++T  A    +  FK IEK+G+NIR+   ++ P  +   G AK IGK
Sbjct: 7   FVFALAVLLALTGQAESKNF--FKRIEKVGKNIRNAAERSLPTVVGYAGVAKQIGK 60


>UniRef50_P01511 Cluster: Cecropin-D; n=6; Obtectomera|Rep:
           Cecropin-D - Antheraea pernyi (Chinese oak silk moth)
          Length = 36

 Score = 41.1 bits (92), Expect = 0.037
 Identities = 15/36 (41%), Positives = 23/36 (63%)
 Frame = +1

Query: 217 WKIFKXIEKMGRNIRDGIVKAGPAIEVLGSAKAIGK 324
           W  FK +E+ G+ +RD I+ AGPA+  +  A A+ K
Sbjct: 1   WNPFKELERAGQRVRDAIISAGPAVATVAQATALAK 36


>UniRef50_Q8MUF4 Cluster: Cecropin-B precursor; n=18; Culicidae|Rep:
           Cecropin-B precursor - Anopheles gambiae (African
           malaria mosquito)
          Length = 60

 Score = 40.3 bits (90), Expect = 0.065
 Identities = 23/63 (36%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
 Frame = +1

Query: 136 MNFAKILSFV-FALVLALSMTSAAPEPRWKIFKXIEKMGRNIRDGIVKAGPAIEVLGSAK 312
           MNF K+   V  A+++ + +      PRWK  K +EK+GRN+     KA P   V+   K
Sbjct: 1   MNFTKLFILVAIAVLVVVGVQPVDGAPRWKFGKRLEKLGRNVFRAAKKALP---VIAGYK 57

Query: 313 AIG 321
           A+G
Sbjct: 58  ALG 60


>UniRef50_Q3R5V3 Cluster: Putative uncharacterized protein
           precursor; n=2; Xylella fastidiosa|Rep: Putative
           uncharacterized protein precursor - Xylella fastidiosa
           Ann-1
          Length = 188

 Score = 33.5 bits (73), Expect = 7.5
 Identities = 24/74 (32%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
 Frame = +1

Query: 85  TAPLVS*RPRLNLHKLTMNFAKILSFVFALVLALSMTSAAPEPRWKIFKXIEKMGRNIRD 264
           ++PL S  PR N   L +     L+    L+LA + T+AAP P       + +M R  + 
Sbjct: 13  SSPLTSPTPRNNAMPLPIAAPLTLATALILLLATTPTTAAPSPEQHAAALVAQMTRQEKI 72

Query: 265 G-IVKAGPAIEVLG 303
              + A PAI  LG
Sbjct: 73  AQTMNAAPAIPRLG 86


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 599,607,522
Number of Sequences: 1657284
Number of extensions: 10396441
Number of successful extensions: 18634
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 15765
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17556
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 81571813589
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)