Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_B10
(873 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 229 1e-60
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 226 1e-59
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 211 4e-55
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 209 2e-54
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 147 7e-36
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 145 4e-35
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 139 2e-33
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 29 4.1
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 29 4.1
At2g44030.1 68415.m05474 kelch repeat-containing F-box family pr... 29 4.1
At4g01330.1 68417.m00173 protein kinase family protein contains ... 29 5.4
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 29 5.4
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 229 bits (561), Expect = 1e-60
Identities = 111/182 (60%), Positives = 130/182 (71%)
Frame = +2
Query: 314 SLXVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPS 493
+L +NG K+ V S+RDP IPW GA+YVVES+GVFTT KA++HL+GGAKKVIISAPS
Sbjct: 150 TLEINGKKVNVVSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKGGAKKVIISAPS 209
Query: 494 ADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXX 673
ADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGLM
Sbjct: 210 ADAPMFVVGVNEHTYQPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGLMTTVHATTATQ 269
Query: 674 XXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPXS*WQADWNGFXVPXANVSXXXS 853
DGPS K WR GRGA QNIIP+STGAAKAVGKV+P + F VP +NVS
Sbjct: 270 KTVDGPSMKDWRGGRGASQNIIPSSTGAAKAVGKVLPELNGKLTGMAFRVPTSNVSVVDL 329
Query: 854 XC 859
C
Sbjct: 330 TC 331
Score = 86.6 bits (205), Expect = 2e-17
Identities = 40/61 (65%), Positives = 47/61 (77%), Gaps = 1/61 (1%)
Frame = +1
Query: 130 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQ 306
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+ KYDSTHG FKGS+ V
Sbjct: 87 TKVGINGFGRIGRLVLRIATSRDDIEVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVI 146
Query: 307 D 309
D
Sbjct: 147 D 147
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 226 bits (553), Expect = 1e-59
Identities = 111/182 (60%), Positives = 129/182 (70%)
Frame = +2
Query: 314 SLXVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPS 493
+L +NG ++ V S+RDP IPW GAEYVVES+GVFTT +AS+HL+GGAKKVIISAPS
Sbjct: 148 TLEINGKQVKVVSKRDPAEIPWADLGAEYVVESSGVFTTVGQASSHLKGGAKKVIISAPS 207
Query: 494 ADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXX 673
ADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGLM
Sbjct: 208 ADAPMFVVGVNEKTYLPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGLMTTVHATTATQ 267
Query: 674 XXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPXS*WQADWNGFXVPXANVSXXXS 853
DGPS K WR GRGA QNIIP+STGAAKAVGKV+P + F VP NVS
Sbjct: 268 KTVDGPSMKDWRGGRGASQNIIPSSTGAAKAVGKVLPELNGKLTGMAFRVPTPNVSVVDL 327
Query: 854 XC 859
C
Sbjct: 328 TC 329
Score = 87.4 bits (207), Expect = 1e-17
Identities = 39/61 (63%), Positives = 48/61 (78%), Gaps = 1/61 (1%)
Frame = +1
Query: 130 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQ 306
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+FKYDSTHG +KG++ V
Sbjct: 85 TKVGINGFGRIGRLVLRIATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVI 144
Query: 307 D 309
D
Sbjct: 145 D 145
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 211 bits (516), Expect = 4e-55
Identities = 108/183 (59%), Positives = 127/183 (69%), Gaps = 1/183 (0%)
Frame = +2
Query: 296 LRFRMDSLXVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKK 472
L+ + D + G K + VF R+P+ IPWG+AGA++VVESTGVFT DKA+AHL+GGAKK
Sbjct: 63 LKVKDDKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 473 VIISAPSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXX 652
V+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGLM
Sbjct: 123 VVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTV 182
Query: 653 XXXXXXXXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPXS*WQADWNGFXVPXA 832
DGPS K WR GR A NIIP+STGAAKAVGKV+P + F VP
Sbjct: 183 HSITATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPSLNGKLTGMSFRVPTV 242
Query: 833 NVS 841
+VS
Sbjct: 243 DVS 245
Score = 78.6 bits (185), Expect = 5e-15
Identities = 34/59 (57%), Positives = 46/59 (77%), Gaps = 1/59 (1%)
Frame = +1
Query: 115 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK 288
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWK 59
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 209 bits (511), Expect = 2e-54
Identities = 107/183 (58%), Positives = 126/183 (68%), Gaps = 1/183 (0%)
Frame = +2
Query: 296 LRFRMDSLXVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKK 472
L+ + + + G K + VF R+P+ IPW +AGA+YVVESTGVFT DKA+AHL+GGAKK
Sbjct: 63 LKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 473 VIISAPSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXX 652
V+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGLM
Sbjct: 123 VVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTV 182
Query: 653 XXXXXXXXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPXS*WQADWNGFXVPXA 832
DGPS K WR GR A NIIP+STGAAKAVGKV+P + F VP
Sbjct: 183 HSITATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTV 242
Query: 833 NVS 841
+VS
Sbjct: 243 DVS 245
Score = 79.0 bits (186), Expect = 4e-15
Identities = 35/67 (52%), Positives = 51/67 (76%), Gaps = 2/67 (2%)
Frame = +1
Query: 115 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK- 288
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 289 GSVEVQD 309
++++D
Sbjct: 61 NELKIKD 67
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 147 bits (357), Expect = 7e-36
Identities = 79/177 (44%), Positives = 100/177 (56%), Gaps = 1/177 (0%)
Frame = +2
Query: 314 SLXVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP- 490
+L V+G I + S+R+P +PWG+ G + V+E TGVF D A HL+ GAKKV+I+AP
Sbjct: 129 ALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQAGAKKVLITAPG 188
Query: 491 SADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 670
D P +VVGVN Y +ISNASCTTNCLAP KV+ F I++G M
Sbjct: 189 KGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKVLDQKFGIIKGTMTTTHSYTGD 248
Query: 671 XXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPXS*WQADWNGFXVPXANVS 841
D L R R A NI+P STGAAKAV V+P + + VP NVS
Sbjct: 249 QRLLDASHRDL-RRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVS 304
Score = 42.3 bits (95), Expect = 4e-04
Identities = 27/59 (45%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
Frame = +1
Query: 133 KIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVE 300
K+ INGFGRIGR LR + VV IND G+ +L KYDST G F V+
Sbjct: 66 KVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTG-GVKQASHLLKYDSTLGIFDADVK 123
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 145 bits (351), Expect = 4e-35
Identities = 79/177 (44%), Positives = 99/177 (55%), Gaps = 1/177 (0%)
Frame = +2
Query: 314 SLXVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPS 493
++ V+G I V S R+P +PW + G + V+E TGVF + A H+E GAKKVII+AP
Sbjct: 126 AISVDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHIEAGAKKVIITAPG 185
Query: 494 -ADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 670
D P +VVGVN AY +ISNASCTTNCLAP KV+ F I++G M
Sbjct: 186 KGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKVLDQKFGIIKGTMTTTHSYTGD 245
Query: 671 XXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPXS*WQADWNGFXVPXANVS 841
D L R R A NI+P STGAAKAV V+P + + VP NVS
Sbjct: 246 QRLLDASHRDL-RRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVS 301
Score = 43.2 bits (97), Expect = 2e-04
Identities = 26/59 (44%), Positives = 33/59 (55%), Gaps = 3/59 (5%)
Frame = +1
Query: 133 KIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVE 300
K+ INGFGRIGR LR + ++AIND G+ +L KYDST G F V+
Sbjct: 63 KVAINGFGRIGRNFLRCWHGRKDSPLDIIAINDTG-GVKQASHLLKYDSTLGIFDADVK 120
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 139 bits (337), Expect = 2e-33
Identities = 79/180 (43%), Positives = 101/180 (56%), Gaps = 3/180 (1%)
Frame = +2
Query: 311 DSLXVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 490
+++ V+G I V S RDP +PW + G + V+E TGVF A H++ GA KVII+AP
Sbjct: 145 ETISVDGKLIKVVSNRDPLKLPWAELGIDIVIEGTGVFVDGPGAGKHIQAGASKVIITAP 204
Query: 491 S--ADAPMFVVGVNLXAYDPSF-KVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXX 661
+ AD P +V+GVN Y +ISNASCTTNCLAP AKV+ + F IV+G M
Sbjct: 205 AKGADIPTYVMGVNEQDYGHDVANIISNASCTTNCLAPFAKVLDEEFGIVKGTMTTTHSY 264
Query: 662 XXXXXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPXS*WQADWNGFXVPXANVS 841
D L R R A NI+P STGAAKAV V+P + + VP NVS
Sbjct: 265 TGDQRLLDASHRDL-RRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVS 323
Score = 45.6 bits (103), Expect = 4e-05
Identities = 27/62 (43%), Positives = 35/62 (56%), Gaps = 3/62 (4%)
Frame = +1
Query: 133 KIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 303
K+ INGFGRIGR LR + +VV +ND G+ +L KYDS G FK V++
Sbjct: 83 KVAINGFGRIGRNFLRCWHGRKDSPLEVVVLNDSG-GVKNASHLLKYDSMLGTFKAEVKI 141
Query: 304 QD 309
D
Sbjct: 142 VD 143
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 29.1 bits (62), Expect = 4.1
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 142 INGFGRIGRLVLRASIEKGAQVVAIND 222
I GFG +G + EKG ++VA++D
Sbjct: 211 IQGFGNVGSWAAKLISEKGGKIVAVSD 237
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 29.1 bits (62), Expect = 4.1
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +1
Query: 142 INGFGRIGRLVLRASIEKGAQVVAIND 222
I GFG +G + EKG +VVA++D
Sbjct: 211 IQGFGNVGTWAAKLIHEKGGKVVAVSD 237
>At2g44030.1 68415.m05474 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 380
Score = 29.1 bits (62), Expect = 4.1
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +2
Query: 167 VWCSVLLLKRELKWSL*MTLSSVLTI 244
+WC+++LL+REL W + +V T+
Sbjct: 331 IWCALILLERELVWGVIEWSENVFTL 356
>At4g01330.1 68417.m00173 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 329
Score = 28.7 bits (61), Expect = 5.4
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -1
Query: 243 IVKTDERVIYSDHLSSLFNRSTEHQTANAAKS 148
I KT+ RV++SD +SS +R T +TA+ + S
Sbjct: 103 IGKTEHRVVFSDRVSSGESRGTVSETASYSGS 134
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 28.7 bits (61), Expect = 5.4
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 133 KIGINGFGRIGRLVLRASIEKGAQVVAIND 222
+ I GFG +G + +KG ++VA++D
Sbjct: 208 RFAIQGFGNVGSWAAKLISDKGGKIVAVSD 237
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,169,764
Number of Sequences: 28952
Number of extensions: 385986
Number of successful extensions: 1119
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1015
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1102
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2048424000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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