Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_A24
(914 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19780.1 68418.m02351 tubulin alpha-3/alpha-5 chain (TUA5) ne... 89 5e-18
At5g19770.1 68418.m02350 tubulin alpha-3/alpha-5 chain (TUA3) ne... 89 5e-18
At4g14960.2 68417.m02299 tubulin alpha-6 chain (TUA6) nearly ide... 87 1e-17
At4g14960.1 68417.m02298 tubulin alpha-6 chain (TUA6) nearly ide... 87 1e-17
At1g50010.1 68414.m05612 tubulin alpha-2/alpha-4 chain (TUA2) id... 87 1e-17
At1g04820.1 68414.m00478 tubulin alpha-2/alpha-4 chain (TUA4) ne... 87 1e-17
At1g64740.1 68414.m07340 tubulin alpha-1 chain (TUA1) nearly ide... 79 5e-15
>At5g19780.1 68418.m02351 tubulin alpha-3/alpha-5 chain (TUA5)
nearly identical to SP|P20363 Tubulin alpha-3/alpha-5
chain {Arabidopsis thaliana}
Length = 450
Score = 88.6 bits (210), Expect = 5e-18
Identities = 52/124 (41%), Positives = 56/124 (45%)
Frame = +1
Query: 310 GVEXIXFXXXXXEXGXGKXVGGXVXVDXEXXVVDEVXXGXXRXLFXXEXXIXGKEDXXXX 489
GV F E G GK V V VD E V+DEV G R LF E I GKED
Sbjct: 43 GVAHDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANN 102
Query: 490 YXXGXXXIGKEXVDLVLXRXXKXXXXCXGXXGXXXXXXXGGGXGXGXXXLLMEXXXVXXG 669
+ G +GKE VDL L R K C G G GGG G G LL+E V G
Sbjct: 103 FARGHYTVGKEIVDLCLDRVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYG 162
Query: 670 KKXK 681
KK K
Sbjct: 163 KKSK 166
Score = 37.9 bits (84), Expect = 0.009
Identities = 17/49 (34%), Positives = 20/49 (40%)
Frame = +3
Query: 183 MXEXXXVXVGXXGVXXGNXCWEXXXXEXGXXXDGXMXXXKXXGGGXDXF 329
M E + +G G+ GN CWE E G DG M G D F
Sbjct: 1 MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGMMPSDTTVGVAHDAF 49
>At5g19770.1 68418.m02350 tubulin alpha-3/alpha-5 chain (TUA3)
nearly identical to SP|P20363 Tubulin alpha-3/alpha-5
chain {Arabidopsis thaliana}
Length = 450
Score = 88.6 bits (210), Expect = 5e-18
Identities = 52/124 (41%), Positives = 56/124 (45%)
Frame = +1
Query: 310 GVEXIXFXXXXXEXGXGKXVGGXVXVDXEXXVVDEVXXGXXRXLFXXEXXIXGKEDXXXX 489
GV F E G GK V V VD E V+DEV G R LF E I GKED
Sbjct: 43 GVAHDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANN 102
Query: 490 YXXGXXXIGKEXVDLVLXRXXKXXXXCXGXXGXXXXXXXGGGXGXGXXXLLMEXXXVXXG 669
+ G +GKE VDL L R K C G G GGG G G LL+E V G
Sbjct: 103 FARGHYTVGKEIVDLCLDRVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYG 162
Query: 670 KKXK 681
KK K
Sbjct: 163 KKSK 166
Score = 37.9 bits (84), Expect = 0.009
Identities = 17/49 (34%), Positives = 20/49 (40%)
Frame = +3
Query: 183 MXEXXXVXVGXXGVXXGNXCWEXXXXEXGXXXDGXMXXXKXXGGGXDXF 329
M E + +G G+ GN CWE E G DG M G D F
Sbjct: 1 MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGMMPSDTTVGVAHDAF 49
>At4g14960.2 68417.m02299 tubulin alpha-6 chain (TUA6) nearly
identical to SP|P29511 Tubulin alpha-6 chain
{Arabidopsis thaliana}
Length = 450
Score = 87.4 bits (207), Expect = 1e-17
Identities = 51/118 (43%), Positives = 54/118 (45%)
Frame = +1
Query: 328 FXXXXXEXGXGKXVGGXVXVDXEXXVVDEVXXGXXRXLFXXEXXIXGKEDXXXXYXXGXX 507
F E G GK V V VD E V+DEV G R LF E I GKED + G
Sbjct: 49 FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHY 108
Query: 508 XIGKEXVDLVLXRXXKXXXXCXGXXGXXXXXXXGGGXGXGXXXLLMEXXXVXXGKKXK 681
IGKE VDL L R K C G G GGG G G LL+E V GKK K
Sbjct: 109 TIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSK 166
Score = 46.4 bits (105), Expect = 3e-05
Identities = 20/49 (40%), Positives = 23/49 (46%)
Frame = +3
Query: 183 MXEXXXVXVGXXGVXXGNXCWEXXXXEXGXXXDGXMXXXKXXGGGXDXF 329
M E + +G G+ GN CWE E G DG M K GGG D F
Sbjct: 1 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAF 49
>At4g14960.1 68417.m02298 tubulin alpha-6 chain (TUA6) nearly
identical to SP|P29511 Tubulin alpha-6 chain
{Arabidopsis thaliana}
Length = 427
Score = 87.4 bits (207), Expect = 1e-17
Identities = 51/118 (43%), Positives = 54/118 (45%)
Frame = +1
Query: 328 FXXXXXEXGXGKXVGGXVXVDXEXXVVDEVXXGXXRXLFXXEXXIXGKEDXXXXYXXGXX 507
F E G GK V V VD E V+DEV G R LF E I GKED + G
Sbjct: 49 FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHY 108
Query: 508 XIGKEXVDLVLXRXXKXXXXCXGXXGXXXXXXXGGGXGXGXXXLLMEXXXVXXGKKXK 681
IGKE VDL L R K C G G GGG G G LL+E V GKK K
Sbjct: 109 TIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSK 166
Score = 46.4 bits (105), Expect = 3e-05
Identities = 20/49 (40%), Positives = 23/49 (46%)
Frame = +3
Query: 183 MXEXXXVXVGXXGVXXGNXCWEXXXXEXGXXXDGXMXXXKXXGGGXDXF 329
M E + +G G+ GN CWE E G DG M K GGG D F
Sbjct: 1 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAF 49
>At1g50010.1 68414.m05612 tubulin alpha-2/alpha-4 chain (TUA2)
identical to tubulin alpha-2/alpha-4 chain SP|P29510
GB:P29510 from [Arabidopsis thaliana]
Length = 450
Score = 87.4 bits (207), Expect = 1e-17
Identities = 51/118 (43%), Positives = 54/118 (45%)
Frame = +1
Query: 328 FXXXXXEXGXGKXVGGXVXVDXEXXVVDEVXXGXXRXLFXXEXXIXGKEDXXXXYXXGXX 507
F E G GK V V VD E V+DEV G R LF E I GKED + G
Sbjct: 49 FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHY 108
Query: 508 XIGKEXVDLVLXRXXKXXXXCXGXXGXXXXXXXGGGXGXGXXXLLMEXXXVXXGKKXK 681
IGKE VDL L R K C G G GGG G G LL+E V GKK K
Sbjct: 109 TIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSK 166
Score = 46.4 bits (105), Expect = 3e-05
Identities = 20/49 (40%), Positives = 23/49 (46%)
Frame = +3
Query: 183 MXEXXXVXVGXXGVXXGNXCWEXXXXEXGXXXDGXMXXXKXXGGGXDXF 329
M E + +G G+ GN CWE E G DG M K GGG D F
Sbjct: 1 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAF 49
>At1g04820.1 68414.m00478 tubulin alpha-2/alpha-4 chain (TUA4)
nearly identical to SP:P29510 Tubulin alpha-2/alpha-4
chain from [Arabidopsis thaliana]
Length = 450
Score = 87.4 bits (207), Expect = 1e-17
Identities = 51/118 (43%), Positives = 54/118 (45%)
Frame = +1
Query: 328 FXXXXXEXGXGKXVGGXVXVDXEXXVVDEVXXGXXRXLFXXEXXIXGKEDXXXXYXXGXX 507
F E G GK V V VD E V+DEV G R LF E I GKED + G
Sbjct: 49 FNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHY 108
Query: 508 XIGKEXVDLVLXRXXKXXXXCXGXXGXXXXXXXGGGXGXGXXXLLMEXXXVXXGKKXK 681
IGKE VDL L R K C G G GGG G G LL+E V GKK K
Sbjct: 109 TIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSK 166
Score = 46.4 bits (105), Expect = 3e-05
Identities = 20/49 (40%), Positives = 23/49 (46%)
Frame = +3
Query: 183 MXEXXXVXVGXXGVXXGNXCWEXXXXEXGXXXDGXMXXXKXXGGGXDXF 329
M E + +G G+ GN CWE E G DG M K GGG D F
Sbjct: 1 MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAF 49
>At1g64740.1 68414.m07340 tubulin alpha-1 chain (TUA1) nearly
identical to SP|P11139 Tubulin alpha-1 chain
{Arabidopsis thaliana}
Length = 450
Score = 78.6 bits (185), Expect = 5e-15
Identities = 45/118 (38%), Positives = 52/118 (44%)
Frame = +1
Query: 328 FXXXXXEXGXGKXVGGXVXVDXEXXVVDEVXXGXXRXLFXXEXXIXGKEDXXXXYXXGXX 507
F E G+ V V +D E V+DEV G R LF E I GKED + G
Sbjct: 49 FNTFFSETSSGQHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHY 108
Query: 508 XIGKEXVDLVLXRXXKXXXXCXGXXGXXXXXXXGGGXGXGXXXLLMEXXXVXXGKKXK 681
+G+E VD L R K C G G GGG G G LL+E V GKK K
Sbjct: 109 TVGREIVDTCLERLRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDFGKKSK 166
Score = 38.3 bits (85), Expect = 0.007
Identities = 17/49 (34%), Positives = 20/49 (40%)
Frame = +3
Query: 183 MXEXXXVXVGXXGVXXGNXCWEXXXXEXGXXXDGXMXXXKXXGGGXDXF 329
M E + +G G+ GN CWE E G DG M G D F
Sbjct: 1 MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGTMPSDSTVGACHDAF 49
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,059,546
Number of Sequences: 28952
Number of extensions: 27484
Number of successful extensions: 61
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 46
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 61
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2168774904
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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