Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_A23
(934 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 229 4e-61
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 127 2e-30
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 106 5e-24
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 81 2e-16
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 9e-12
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 64 3e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 56 5e-09
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 28 1.6
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 27 2.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 229 bits (560), Expect = 4e-61
Identities = 100/115 (86%), Positives = 107/115 (93%)
Frame = +2
Query: 188 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXK 367
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEA K
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 368 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPL PK R
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNR 116
Score = 68.9 bits (161), Expect = 9e-13
Identities = 33/44 (75%), Positives = 36/44 (81%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM QI+FE FN+P YV IQAVLSLYASGRT GIVL GDGV+
Sbjct: 117 EKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVT 160
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 127 bits (307), Expect = 2e-30
Identities = 58/108 (53%), Positives = 75/108 (69%), Gaps = 1/108 (0%)
Frame = +2
Query: 194 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXKRG 373
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EA RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 374 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPL 514
+L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL
Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115
Score = 46.4 bits (105), Expect = 6e-06
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EK+ + +E N P + +Q VL+LYAS RT GIVL GDG++
Sbjct: 122 EKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLT 165
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 106 bits (254), Expect = 5e-24
Identities = 50/113 (44%), Positives = 74/113 (65%), Gaps = 2/113 (1%)
Frame = +2
Query: 200 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAHXKRG 373
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 374 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
+L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+ P R
Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANR 116
Score = 51.6 bits (118), Expect = 2e-07
Identities = 24/44 (54%), Positives = 31/44 (70%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM + MFE + +YV IQAVLSLYA G + G+V+ GDGV+
Sbjct: 117 EKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVT 160
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 81.0 bits (191), Expect = 2e-16
Identities = 49/133 (36%), Positives = 67/133 (50%), Gaps = 24/133 (18%)
Frame = +2
Query: 206 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 334
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 335 ------DSYVGDEAHXKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 493
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 494 LLTEAPLXPKXXR 532
LLTE PL P R
Sbjct: 127 LLTEPPLNPPENR 139
Score = 35.5 bits (78), Expect = 0.011
Identities = 21/52 (40%), Positives = 29/52 (55%), Gaps = 8/52 (15%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRT--------XGIVLXXGDGVS 665
E +IMFE FN +Y+ +QAVL+L AS + G V+ GDGV+
Sbjct: 140 ENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVT 191
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 65.7 bits (153), Expect = 9e-12
Identities = 38/120 (31%), Positives = 67/120 (55%), Gaps = 1/120 (0%)
Frame = +2
Query: 176 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 355
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR----KLSRTSTLVGND 72
Query: 356 AHXKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
+ G ++ + P E +I+NWD ME++ +TF +L + EHP+ +TE P R
Sbjct: 73 TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131
Score = 29.1 bits (62), Expect = 0.94
Identities = 14/44 (31%), Positives = 25/44 (56%), Gaps = 2/44 (4%)
Frame = +3
Query: 540 MXQIMFEXFNSPXMYVXIQAVLSLYASGR--TXGIVLXXGDGVS 665
M +++FE +N+P + I + S Y + + + GIVL G+ S
Sbjct: 134 MTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAAS 177
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 64.1 bits (149), Expect = 3e-11
Identities = 33/105 (31%), Positives = 55/105 (52%)
Frame = +2
Query: 191 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXKR 370
D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI----YKSN 60
Query: 371 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 505
+ +K I +G + NWD +W + +L+ P EHP+L+TE
Sbjct: 61 PGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITE 105
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 56.4 bits (130), Expect = 5e-09
Identities = 32/116 (27%), Positives = 59/116 (50%), Gaps = 2/116 (1%)
Frame = +2
Query: 191 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXKR 370
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 371 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
I + +K +GII +W+ W +L+V P E+ +++TE P+ R
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVR 117
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 28.3 bits (60), Expect = 1.6
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +2
Query: 251 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXKRGILTLKYP 394
G+ PRA F ++ P H G+++ M KD G+E +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 27.5 bits (58), Expect = 2.9
Identities = 26/104 (25%), Positives = 46/104 (44%), Gaps = 4/104 (3%)
Frame = +2
Query: 206 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXKRGILTL 385
+V+DNG+ KAGFAG P+ + R + G + ++G+E TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 386 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTE 505
++ H G + +W +W N + P ++ +LLT+
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQ 101
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,053,030
Number of Sequences: 5004
Number of extensions: 32058
Number of successful extensions: 74
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 59
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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